생식소자극호르몬방출호르몬 類似物質에 의한 메기(Silurus asotus)의 배란유도

권혁추(Hyuk-Chu Kwon),최낙중(Nack-Joong Choi),박홍양(Hong-Yang Park) 한국수산과학회 양식분과 1996 韓國養殖學會誌 Vol.9 No.3

뱀장어의 in vitro Vitellogenin 합성에 대한 Estradiol 과 뇌하수체 호르몬의 영향

권혁추(Hyuk Chu Kwon) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.2

Hepatocytes of Anguilla japonica have been prepared using a collagenase perfusion technique. The isolated cells attached efficiently to fibronectin-coated culture dishes and subsequently formed monolayers in serum-free medium. These cultures maintained in appropriate medium at least for 10 days with minimal cell loss. The effects of estradiol and pituitary hormones on vitellogenin (Vg) synthesis were examined in primary hepatocyte culture of the immature eels. In fish, as in other oviparous vertebrates, estrogen is a major inducer of Vg synthesis. However, estradiol-17β(E₂) alone was insufficient to induce Vg synthesis in cultures of eel hepatocytes. Combination of E₂with growth hormone (GH) and/or prolactin (PRL) markedly stimulated Vg synthesis. Even in cultures exposed to E₂or precultured without hormones for 8 days, E₂alone could not fully induce Vg synthesis. The synthesis of Vg was dramatically increased when hepatocytes were cultured in medium supplemented with E₂+GH+PRL for 6 days. At this point, even though GH and/or PRL were eliminated from the medium, Vg synthesis was not influenced by these factors during culture of further 3 days. These results indicate that pituitary hormones, in particular GH and PRL, play important roles in the regulation of Vg synthesis in primary cultures of eel hepatocytes.

어류혈청이 메기(Silurus asotus) 간세포의 단층배양에 미치는 영향

권혁추 ( Hyuk Chu Kwon ),최성희 ( Seong Hee Choi ),김은희 ( Eun Hee Kim ),한덕우 ( Deug Woo Han ),권준영 ( Joon Yeong Kwon ) 한국수산과학회 2006 한국수산과학회지 Vol.39 No.1

뱀장어 배양 간세포에서의 Cytochrome P4501A (CYP1A) 유전자 발현에 대한 중금속들의 억제효과

권혁추 ( Hyuk Chu Kwon ),맹준호 ( Joon Ho Maeng ),최성희 ( Seong Hee Choi ) 한국어병학회 2010 한국어병학회지 Vol.23 No.2

Effects of heavy metal ions on the gene expression of cytochrome P4501A (CYP1A) were examined in cultured eel hepatocytes. When the expression of CYP1A mRNA was measured by RT-PCR after incubation of eel hepatocytes with benzo[α]pyrene (B[α]P) at concentrations of 10-8∼10-5 M, the CYP1A expression increased with B[α]P treatment in a dose dependent manner, showing significant increase at concentrations more than 10-7 M. When the eel hepatocyte was treated with cadmium (10-6 and 10-5 M), the expression of CYP1A was inhibited and especially at higher concentration (10-5 M). The inhibition of CYP1A expression by cadmium was also observed in cells treated with B[α]P. In another study, effects of heavy metal ions on the expression of CYP1A were examined in cultured hepatocytes isolated from eel which was treated previously with B[α]P in vivo. Hepatocytes isolated from the liver of eel taken at 48 hours after injection of B[α]P (10 mg/kg) were cultured for 2 days with cadmium, copper, lead or zinc (10-6 and 10-5 M). The expression of CYP1A was found to be suppressed by the metal ions compared with the control in which CYP1A was induced with previous treatment of B[α]P in vivo. The present results may provide an important basic information for studying the effects of heavy metal ions on CYP1A expression in other species of fish and studying toxicological mechanisms of heavy metal ions in aquatic livings.

웅성호르몬에 의한 무지개송어의 vitellogenin 유전자 발현

권혁추(Hyuk Chu Kwon),윤종만(Jong Man Yoon),이종영(Jong Young Lee) 한국수산과학회 양식분과 2000 韓國養殖學會誌 Vol.13 No.1

Influences of Parental Pairs on Progeny Sex Ratios of Nile Tilapia Oreochromis niloticus

Joon Yeong Kwon(권준영),Hyuk Chu Kwon(권혁추),David J. Penma 한국수산과학회 양식분과 2006 韓國養殖學會誌 Vol.19 No.2

어류 Vitellogenin의 간단한 측정방법

권혁추 선문대학교 자연과학대학 1999 자연과학대학 논문집 Vol.2 No.-

성숙한 충거리가자미 암컷혈청으로부터 Mg-EDTA침전법 및 Sepharose 6B column chromatography에 의해 Vg를 분리하여, 항혈청을 제작한 후 면역확산법을 이용하여 Vg을 측정하였다. 1996년 12월부터 1997년 4월에 걸쳐 조사한 충거리가자미의 생식소 숙도지수와 혈중 Vg농도는 서로 밀접한 관계를 가지는 것으로 나타났다. 1996년 1월하순까지 혈중 Vg농도는 약 lmg/ml였으며, GSI는 6-10%로 서서히 증가하기 시작했다. 3월초에 혈중 Vg농도는 약 8mg/ml, GSI는 12~19%로 급격한 증가를 보였다. 3월중순에서 하순에 걸쳐 혈중 Vg은 최고치를 나타내었고 (14.48±3.54mg/ml), GSI도 27.3%로 가장 높았다. 간숙도지수는 성숙기간중 2.2~2.5%로 커다란 변화를 보이지 않았으나 4월하순에는 1.7%로 감소하였다. Serum vitellogenin of sand flounder, Limanda punctatissima was purified by precipitation with Mg-EDTA, followed by Sepharose 6B column chromatography. The Vg level in serm during the sexual maturation (December 1996 to April 1997) was measured by the single radial immunodiffusion. This technique is sufficient to quantify circulating serum Vg during the later stages of exogenous vitellogenesis. The Vg level (0.5~1.0mg/ml) and GSI (6~10%) rose slowly but gradually from December 1996 to January 1997. A sharp increase of the Vg level(8mg) was observed in early March and it reached a maximum value (14.48±3.54mg/ml) in mid-March. Maximum GSI (27.3%) were also observed in mid-March, while the HSI (2.2-2.5%) was not changed during the reproductive cycle.

GnRH-analogue와 pimozide에 의한 메기의 배란유도

권혁추 선문대학교 자연과학대학 1998 자연과학대학 논문집 Vol.1 No.-

메기의 배란유도에 대한 GnRH-a, pimozide, HCG 및 PMSG 효과를 조사하였다. GnRH-a를 주사한 개체들의 대부분(64-100%)은 pimozide에 관계없이 배란되었다. GnRH-a 120㎍/kg을 주사한 개체들에서 70% 배란유도 효과를 나타냈으나, GnRH-a 100-120㎍/kg과 pimozide 500㎍/kg 혼합 주사에서는 100%를 보여, pimozide가 GnRH-a 의 활성을 높여주는 것으로 나타났다. pimozide만으로는 배란은 유도되지 않았다. 한편, HCG 단독에 의해서도 배란이 유도되었는데, 높은 HCG 농도(20,00IU/kg)일지라도 GnRH-a와 pimozide 혼합투여에 비해 낮은 배란유도효과를 나타냈다. 또한 HCG 단독보다는 PMSG를 미리 주사한후 HCG사용하는 것이 배란유도 효과가 높았다. Experiments were carried out to investigate the effect of GnRH-a alone or in combination with pimozide, HCG or PMSG on the induction of ovulation in S. asotus. The majoriry (64-100%) of catfish injected with GnRH-a were ovulated regardless of pimozide presence. Ovulation was induced in 70% at a dose of GnRH-a 120㎍/kg of body weight. Pimozide potentiated the activity of GnRH-a, the most effective combination for inducing ovulation (100%) was GnRH-a at 100-120㎍/kg plus pimozide at 500㎍/kg- Pimozide alone or saline was ineffective in inducing ovulation. On the other hand, fish injected with HCG were also ovulated, but the rate was lower than that of injection with GnRH-a plus pimozide although HCG dosages were high (20,000IU/kg). Injection of HCG (10,000-20,00IU/kg) to PMSG-primed (l,000IU/kg) catfish resulted in significant increase in ovulation rate, compared to only HCG-injection.

Induced ovulation in Korean freshwater fish using LHRH-a combined with pimozide

Hyuk, chu-kwon 선문대학교 중소기업기술지원연구소 2001 선문공대 연구/기술 논문집 Vol.6 No.1

한국산 담수어류, 메기, 동자개 및 미꾸라지의 배란 및 산란유도를 촉진하기 위한 효율적이고 경제적인 방법을 개발하기 위해, LHRH-a, HCG, PMSG 및 도파민 길항제인 pimozide의 단독 또는 혼합투여에 의한 효과를 비교 조사하였다. 실험된 모든 어류에서 HCG 또는 HCG+PMSG에서 쉽게 배란이 유도되었다. LHRH-a 단독투여 역시 배란유도에 효과적이었으며, pimozide는 LHRH-a의 효과를 증강시켰다. LHRH-a + pimozide의 혼합투여가 배란유도에 가장 효과적이었다. LHRH-a + pimozide의 혼합투여는 HCG 단독 또는 HCG+PMSG 혼합투여보다 비용면에서 훨씬 경제적인 것으로 나타났다. In order to develop a simple and cost-effective method for accelerating and synchronizing ovulation and spawning in Korean catfish, Silurus asotus, Korean bullhead, Pseudobagrus fulvidraco and loach, Misgurnus mizolepis, the effectiveness of LHRH-a. HCG and PMSG alone or in combination with dopamin antagonists, pimozide was investigated. Ovulation was easily induced by injection of HCG alone or by HCG plus PMSG in experimented fish. A single injection of LHRH-a alone was also effective to induce ovulation in these fish. Pimozide enhanced the activity of LHRH-a, the most effective combination for inducing ovulation was LHRH-a plus pimozide. The technique using LHRH-a combined with pimozide is more economical than HCG alone or HCG+PMSG.

LHRH-a와 pimozide에 의한 동자개의 배란유도

박홍양,이종영,이영직,권혁추 한국양식학회 1998 韓國養殖學會誌 Vol.11 No.2

동자개의 배란유도에 대한 LHRH-a, pimozide 및 HCG의 복강내 투여효과에 대해 조사하였다. 어체중 ㎏당 HCG를 각각 5,000, 10,000, 20,000 IU를 주사하였을 경우 71.4, 80, 100%의 배란유도율을 나타냈다. 또한 pimozide의 존재에 관계없이 LHRH-a를 주사한 개체들 대부분이 배란되었다. LHRH-a 단독투여 실험에서 ㎏당 200, 300, 400 ㎍처리에서 각각 50, 75 및 100%의 배란유도율을 나타냈다. 즉, LHRH-a와 pimozide의 혼합투여에서 어체중 ㎏당 pimozide 1㎎과 LHRH-a 300㎍에서 100% 가장 좋은 배란유도율을 나타내었다. 그러나 pimpzide 단독투여에서는 배란이 유도되지 않았다. 호르몬 처리후의 생식소숙도지수(GSI)와 pseudo-GSI의 변화를 조사한 결과, HCG, LHRH-a 및 LHRH-a + pimozide를 주사한 그룹간의 생식소숙도지수(GSI)와 pseudo-GSI에 있어서 통계적인 유의차는 없었다.(p>0.05) 호르몬 처리후 배란이 일어나기까지의 소요시간은 HCG 처리후 17∼22시간, LHRH-a 단독 또는 pimozide와의 혼합처리에서 대체로 23∼29시간이 걸렸다. Effects of intraperitoneal administration of LHRH-a (luteinizing hormone releasing hormone analogue), pimozide and HCG (human chorionic gonadotropin) were studied on ovulation in the bullhead, Pseudobagrus. fulvidraco. Ovulation was induced in 71.4, 80 and 100% by injection of HCG 5,000, 10,000 and 20,000 IU/㎏ of body weight, respectively. Also, the majority of fish injected with LHRH-a in the presence or absence of pimozide were ovulated. The ovulating rates in fish injected with LHRH-a alone showed 50, 75 and 100% at a dose 200, 300 and 400 ㎍/㎏, respectively. Pimozide enhanced the activity of LHRH-a, the most effective combination for inducing ovulation (100%) was LHRH-a at 300 ㎍/㎏ plus pimozide at 1 ㎎/㎏. Pimozide alone was ineffective in inducing ovulation of P. fulvidraco. There were no significant differences in gonadosomatic index (GSI) and pseudo-GSI values among the groups treated with HCG, LHRH-a and LHRH-a plus pimozide (p>0.05). The latency time of group treated with HCG was 17∼22 hours, and the group injected with LHRHa alone and/or in combination with pimozide was ovulated within 23∼29 hours.