냉동 건조 탈회 동종골 이식후 골막이 골 형성 과정에 미치는 영향에 관한 실험적 연구

권혁도,이동근 원광대학교 치의학연구소 1995 圓光齒醫學 Vol.5 No.2

Periosteum in general is described as a specialized fibrous membrane of mesenchymal origin consisting of two basic layers: outer fibrous layer consists of irregularly arranged dense connective-tissue with fibroblasts, and inner osteogenic or cambial layer is composed of more loosely arranged fibers, greater vascularity and flattened spindle-shaped pre-osteoblasts. This periosteum may serve in controlling bone growth, especially mandibular growth has been emphasized. But, the periosteum enwrapping the facial skeleton have been studied for many years leaving a controversy in opinion regarding the function of these structures. We evaluated the bone formation activity of the periosteum in allogeneic bone grafts which bones are made of freeze-dried preparation preoperatively. We made the calvarial bone defects, 5×7㎜ sized, and grafted with allogeneic bone in rats, which a half of specimens has dissected the overlying periosteum and a rest intacted. After bone grafting, we evaluated the capacity of bone formation of periosteum, 1, 2, 4, 6, 8 weeks postoperatively. There are subtle differences of bone formation during early healing period after demineralized allogeneic bone grafting between control groups with periosteum and experimental groups without periosteum.

오늘, 이 순간이 바로 나의 삶이다

권혁도 샘터사 2003 월간 샘터 Vol.34 No.2

흰가루병 방제용 기능성 미생물 연구

권혁도 춘천바이오산업진흥원 2020 CBF 바이오경제 이슈포커스 Vol.2020 No.8

기능성 미생물을 이용한 폐혈액 자원화 기술개발

권혁도 춘천바이오산업진흥원 2019 CBF 바이오경제 이슈포커스 Vol.2019 No.4

백서 두개골 결손부에 Hydroxylapatitie와 TGF-β 매식 후 치유과정에 관한 연구

권혁도,이동근,김은철,Kwon, Hyuk-Do,Lee, Dong-Kuen,Kim, Eun-Chol 대한악안면성형재건외과학회 1999 Maxillofacial Plastic Reconstructive Surgery Vol.21 No.1

The purpose of this study was to evaluate the healing process of the calvarial defect filled with hydroxylapatite(HA) and $TGF-{\beta}$ in Rat. 72 Sprague-Dawly rats were divided into 3 groups, control and two experimental groups. Bony defect were artificially prepared in the calvaria of all 72 rats and followed by implantation of HA (experimental group of 24 rats) and HA+$TGF-{\beta}$(another experimental group of 24 rats) into the defects. Sequential sacrifice was performed at 1, 2, 4, 6, 8, 12 weeks of experiment. Obtained specimen was stained with Hematoxylin and Eosin, Masson's Trichrome and Immunohistochemistry. The results were as follows, 1. Granulation tissue was prominent on control group in 1 and 2 weeks. Bony defects were filled with dense fibrous tissue through the whole experimental period and osteoinduction could not be observed in all groups. 2. Inflammatory cell infiltration was prominent on control group in 1 and 2 weeks and osteoclastic activity was high in HA implanted experimental group at 1 and 2 weeks. 3. Inflammatory cell infiltration was less and maturation of fibrous tissue could be found on HA+$TGF-{\beta}$ implanted experimental group at 1 and 2 weeks. 4. Osteoconduction activity was high in HA+$TGF-{\beta}$ implanted experimental group at 2 and 4 weeks but there was no difference after 6 weeks among 3 groups. 5. In grafted site of HA+$TGF-{\beta}$ implanted group, osteonectin expression was slightly increased from 1 week to 6 weeks. In the host site, it was increased from 1 to 4weeks. 6. In grafted site of HA+$TGF-{\beta}$ implanted group, osteocalcin expression was high at 4 weeks. In the host site, we could find the difference among 3 groups. From above results, the HA with mixture of $TGF-{\beta}$ has the potentiality of promoting bone formation in the bony defect area in the rat.

Interactions between Indole-3-acetic Acid Producing Acinetobacter sp. SW5 and Growth of Tomato Plant

권혁도,송홍규,Kwon, Hyeok-Do,Song, Hong-Gyu The Microbiological Society of Korea 2014 미생물학회지 Vol.50 No.4

Many rhizobacteria can promote plant growth through various direct or indirect mechanisms, and their production of phytohormones such as indole-3-acetic acid (IAA) may have pronounced effects on growth and development of plants. Rhizobacterial strain isolated from rhizosphere of foxtail (Setaria viridis), Acinetobacter sp. SW5 produced 118.1 mg/L of IAA and 4.5 mg/L of gibberellin ($GA_3$) in brain heart broth medium at 2 and 1 day of incubation, respectively. In a pot test the lengths of stem and root and fresh weight of the germinated tomato seedlings treated with Acinetobacter sp. SW5 significantly increased by 26.3, 33.3, and 105.3%, respectively compared to those of the uninoculated control in 12 weeks of cultivation. When the root exudate secreted from tomato seedlings was analyzed by HPLC, 3.75 ng mg tomato $root^{-1}$ of tryptophan which is an IAA precursor was detected. Acinetobacter sp. SW5 could produce $4.06{\mu}M$ of IAA from root exudate from 8 tomato seedlings. Together with the capability of growth of Acinetobacter sp. SW5 in the tomato root exudates, this IAA secreted by bacteria might contribute to enhance the growth of tomato plants. 많은 근권세균들이 다양한 직간접적인 방법을 통해 식물생장을 촉진할 수 있으며 indole acetic acid (IAA) 같은 식물호르몬의 생산과 분비는 식물의 생장과 발달에 큰 영향을 미친다. 강아지풀의 근권에서 분리된 Acinetobacter sp. SW5는 BHB 배지에서 각각 2일과 1일 배양 시 118.1 mg/L의 IAA와 4.5 mg/L의 지베렐린을 생성하였다. 소규모 재배실험에서 토마토 유묘에 이 균주를 2주 간격으로 처리하면서 12주 재배 시 토마토 식물의 shoot와 뿌리 길이 및 습윤중량이 비접종 대조군과 비교할 때 통계적으로 유의하게 각각 26.3, 33.3과 105.3% 증가하였다. 토마토 유묘의 뿌리로부터 분비되는 삼출물을 HPLC로 분석한 결과 IAA의 전구물질인 아미노산 트립토판이 3.75 ng/mg tomato root 검출되었으며 Acinetobacter sp. SW5는 8개의 토마토 유묘뿌리 삼출물로부터 $4.06{\mu}M$의 IAA를 생성하였다. 토마토 뿌리 삼출물에서 Acinetobacter sp. SW5의 생장능과 더불어 이 세균에 의해 생성된 IAA가 토마토 식물의 생장을 촉진시키는데 관여했을 것으로 추정된다.

경인운하 갑문에 관한 수리모형실험

권혁도,김규한 한국수자원학회 1998 한국수자원학회 학술대회 Vol.1998 No.1

수확시기별 첨가제 수준에 따른 사료용 벼사일리지의 TDN 함량 추정

권혁도,홍석만,성경일,김병완,김민기,최종우,김아정 한국초지조사료학회 2002 한국초지조사료학회 학술대회논문집 Vol.2002 No.1

냉동 건조 탈회 동종골 이식후 골막이 골 형성 과정에 미치는 영향에 관한 실험적 연구

이동근,엄인웅,민승기,권혁도 大韓顎顔面成形再建外科學會 1995 Maxillofacial Plastic Reconstructive Surgery Vol.17 No.4

Periosteum in general is described as a specialized fibrous membrane of mesenchymal origin consisting of two basis layers : outer fibrous layer consists of irregularly arranged dense connective-tissue with fibroblasts, and inner osteogenic or cambial layer is composed of more loosely arranged fibers, greater vascularity and flatted spindle-shaped pre-osteoblasts. This periosteum may serve in controlling bone growth, especially mandibular growth has been emphasized. But, the periosteum enwrapping the facial skeleton have been studied for many years leaving a controversy in opinion regarding the function of these structures. We evaluated the bone formation activity of te periosteum in allogeneic bone grafts which bones are made of freeze-dried preparation preoperatively. We made the calvarial bone defects, 5×7mm sized, amd grafted with allogeneic bone in rats, which a half of specimens has dissected the overlying periosteum and a rest intacted. After bone grafting, we evaluated the capacity ofbone formation of periosteum, 1, 2, 4, 6, 8 weeks postoperatively. There are subtle differences of bone formation during early healing period after demineralized allogeneic bone grafting between control groups with periosteum and experimental groups without periosteum.

수환경에서 분리한 세균 균주들의 식물생장촉진능

권혁도(Hyeok-Do Kwon),송홍규(Hong-Gyu Song) 강원대학교 환경연구소 2010 Journal of the Environment Vol.7 No.-

Environmental concern over conventional agricultural fertilization and disease control measures has led to increased interests in finding the environment friendly alternatives including the plant growth promoting bacteria. In this study some photosynthetic bacteria and heterotrophic bacteria were isolated from the aquatic environments. Phylogenetic analyses based on 16s rDNA gene sequences showed that the selected strains JFR, CR, JJ3 and DB are belonged to Rhodobacter blasticus, Rhodobacter johrii, Klebsiella veriicola and Proteus vulgaris, respectively, sharing 98.224, 98.672, 98.452 and 99.717% of sequence similarity with the closest neighbor of each type strain. They are able to exert the beneficial effects upon plant growth by production of phytohormones. Among the isolates, R. blasticus JFR showed the highest production rate of 1,388.102 ㎍ ㎎ protein-¹ of indole acetic acid, 1,251.375 ㎍ ㎎ protein-¹ of indole butyric acid in the modified Biebl and Pfenning medium. R. johrii JJ3 showed the soluble phosphate production of 536.3 ㎎ L-¹ in 5 days of cultivation in LB medium. The production rates of 5-aminolevulinic acid by R. blasticus JFR and R. johrii CR in LB medium were 2.54 and 0.96 ㎍ ㎎ protein-¹, respectively in 1 day. Dry weight of germinated tomato seedling inoculated with R. blasticus JFR was increased by 85.7% (p<0.005) than that of the control in 21 days. These photosynthetic bacteria and heterotrophic bacteria may be utilized as environment-friendly biofertilizer in the agriculture.