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권오남,박흠기,Kwon O-Nam,Park Heum-Gi 한국양식학회 2005 韓國養殖學會誌 Vol.18 No.4
본 연구는 rotifer, B. rotundiformis를 대상으로 소화효소 실험을 하기 위해 이들이 가지고 있는 소화효소의 최고 활성 조건을 확인하기 위해 수행하였다. rotifer, B. rotundiformis의 $\alpha$-amylase, total alkaline Protease, trypsin 및 TG-lipase는 Tris-HCl buffer 보다 phosphate-NaOH buffer 안정적인 효소활성을 보였다. $\alpha$-amylase, trypsin 및 TG-lipase는 pH 8.0에서, total alkaline proteaset pH 7.0에서 높은 효소 활성을 나타내었다. $\alpha$-amylase 활성은 $40^{\circ}C$에서 가장 높은 활성을 보였으며, total alkaline pretense와 trypsin은 $55{\~}60^{\circ}C$의 온도에서 높은 활성을 나타내었다. 반면 TG-lipase 활성은 $25{\~}30^{\circ}C$의 낮은 온도에서 활성이 높았다. $\alpha$-amylase, total alkaline pretense, trypsin 및 TG-lipase의 활성의 적정 기질 농도는 $3.5\%$ starch, $\0.6%$ azo-casein, $87.5{\mu}M$ BApNA and 81.2 mM olive oil이었다. $\alpha$-amylase, total alkaline protease, trypsin 및 TG-lipase의 활성의 적정 반응시간은 40, 60, 30 and 25 min으로 나타났다. 본 연구 결과에서 얻어진 자료는 rotifer, B. rotundiformis의 소화효소 연구를 위한 기초 자료로 이용될 것이다. This study was investigated the condition of their maximum activity to assay the enzymes of rotifer, Brachionus rotundiformis의 $\alpha$-amylase, total alkaline Protease, trypsin and TG-lipase activities of rotifer were higher and more sensitive in phosphate-NaOH buffer than Tris-HCl buffer. $\alpha$-amylase, trypsin and TG-lipase activities were appeared the maximum at pH 8.0, and total alkaline protease activity showed the maximum activity at pH 7.0. $\alpha$-amylase activity showed the highest activity at $40^{\circ}C$, and total alkaline protease and trypsin activities were assayed the highest at $55{\~}60^{\circ}C$. However, TG-lipase activity was appeared the highest at $25{\~}30^{\circ}C$. The optimum substrate concentration of enzyme activity of a-amylase, total alkaline protease, rypsin and TG-lipase were $3.5\%$ starch, $\0.6%$ azo-casein, $87.5{\mu}M$ BApNA and 81.2 mM olive oil, respectively. The optimum reaction time of enzyme activity of $\alpha$-amylase, total alkaline protease, trypsin and TG-lipase were increased up to 40, 60, 30 and 25 min., respectively. The data obtained in this study could be used for the digestive enzyme research of rotifer, B. rotundiformis.
수온에 따른 명태(Theragra chalcogramma) 수정란의 부화 패턴과 부화 자어의 기형 발생에 미치는 영향
권오남 ( O-nam Kwon ),홍우석 ( Woo-seok Hong ),박진철 ( Jin-chul Park ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.6
To find the optimal eggs to larvae transforming temperature of walleye pollock Theragra chalcogramma, we investigated their hatching pattern, mortality, and larval deformities. Results showed that the hatching quality decreased as the water temperature increased and dropped sharply above 13℃. The highest hatching rate was achieved at 7℃, whereas the lowest was at 15℃. Dead eggs were not observed at 7℃; however, dead egg ratios were significantly high at 4.5℃ and above 13℃ (P<0.05). Mortality of larvae after hatching was not observed at 4.5℃, but was significantly high above 13℃. The DNA content of the larvae did not show any significant difference at all water temperatures; however, RNA content was the highest at 7℃ and was significantly low above 11℃. In addition, RNA/DNA ratio was the highest at 7℃. Larval deformities after hatching were low at 7-11℃ and higher at higher or lower water temperature. Therefore, the hatching index and larval health index of Walleye pollock eggs in this study show that 7℃ to 11℃ seems to be the optimal transforming water temperature range.
물렁가시붉은새우(Pandalopsis japonica) 부화유생 사육을 위한 영양강화 알테미아, 바지락살 및 배합사료의 공급 효과
권오남 ( O Nam Kwon ),임매순 ( Mae Soon Lim ),박기영 ( Kie Young Park ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.5
Larvae of pandalid shrimp Pandalopsis japonica are benthic, and may prefer bottom to planktonic food items. Three diets, enriched Artemia meta-nauplii (AR), clam meat (CM), and crumbled shrimp (CD), were evaluated as food for early larvae in terms of biometric growth R:D ratios. Larvae fed AR and CM had significantly greater total length and dry weight than did larvae fed CD (P<0.05). Survival was significantly greater in larvae fed CM than in those fed AR and CD (P<0.05). R:D ratios were also significantly higher in larvae fed AR and CM than in those fed CD (P<0.05). Planktonic Artemia meta-nauplii containing high levels of fatty acids and amino acids were not suitable for either normal somatic growth or survival at early benthic larval stages. Furthermore, it is necessary to determine the optimal feeding duration to increase digestibility of AD. Thus, we propose that clam meat is the best diet for pandalid shrimp larvae.
Vitamin B12 첨가가 담수산 rotifer , Brachionus calyciflorus Pallas 의 내구란의 생산과 부화율에 미치는 영향
권오남(O Nam Kwon),조수근(Soo Gun Jo),박흠기(Heum Gi Park) 한국수산과학회 2001 한국수산과학회지 Vol.34 No.2
본 연구는 vitamin B_(l2)의 첨가에 따른 담수산 rotifer, Brachionus calyciflorus의 유성생식율 및 내구란 생산을 조사하였다. Vitamin B_(12)의 첨가방법은 rotifer의 먹이생물인 농축 담수산 Chlorella에 첨가하여 공급하였고 실험용기는 250mL 삼각플라스크에서 실시하였다. Rotifer의 최고밀도는 vitamin B-(12)의 첨가량이 높을수록 낮게 나타났고 6 ppm구에서 가장 낮은 138개체/mL를 보였다. 유성생식율과 수정률은 vitamin B_(12)의 첨가량이 많을수록 높아 6ppm구에서 각각 63.0%와 89.6%로 조사되었다. mL당 내구란 생산은 1.5ppm구에서 231개/mL로 가장 높게 나타났으나 다른 vitamin B_(12)의 첨가구와의 유의적인 차이는 없었고, Rotifer 10⁴개체당, Chlorella 건중량 (g) 당 내구란 생산량은 6 ppm구에서 각각 11,289개, 121,252개로 가장 높게 나타났다. Vitamin B_(12)의 첨가량에 따라 형성된 내구란의 부화율은 vitamin B_(12)를 첨가한 실험구에서 67.7∼80.9%의 부화율을 보여 첨가하지 않은 대조구 48.2% 보다 높게 나타났다. 따라서 담수산 rotifer, B. calyciflorus의 내구란 생산과 내구란의 부화율에 vitamin B_(12)가 영향을 미친 것으로 판단된다. This study was performed to investigate the effect of vitamin B_(12) addition on the production and hatching rate of resting egg of freshwater rotifer, Brachionus calyciflorus. Different concentrations of vitamin B_(12) was added into concentrated freshwater Chlorella before fed to rotifer and rotifer was cultured in 250 mL flask. The maximum density of rotifer was 138 inds./mL at 6 ppm and it increased with an increase of vitamin B_(12) addition. Mixis rate and fertilization rate of rotifer were 63.0% and 89.6%, respectively, at 6.0 ppm. The production of resting eggs per mL was 231 eggs/mL at 1.5 ppm, but there was no significant difference among vitamin B_(12) addition levels. The highest production of resting eggs per 10,000 rotifers and Chlorella dry weight (g) were 11,289 eggs and 121,252 eggs, respectively, at the 6 ppm. The hatching rate of resting egg was not significantly different among vitamin B_(12) addition levels, but significantly higher than the control (without addition of vitamin B_(12)). These results showed that vitamin B_(12) addition could affect production and hatching rate of resting egg of freshwater rotifer, B. calyciflorus.