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Canavanine 이 보리종자에서 GA3 에 의해 유도되는 Amylase 활성증가에 미치는 영향
박인호,권영명 ( In Ho Park,Young Myung Kwon ) 생화학분자생물학회 1981 BMB Reports Vol.14 No.3
The effect of L-canavanine on the GA₃ -induced amylase activity was studied in barley half seed (Hordeum vulgare L. cv. Baecdong). Canavanine, 2 mM, inhibited completely the activity of amylase and the content cf soluble protein induced by GA₃. And 2 electrophoretic isozyme binds also disappeared. This inhibition was recavared by the addition of arginine. Inhibitory effect of canavanine on the enzyme activity was altered according to different time cf the addition during 24 hr of incubation of the half seeds with GA₃. The addition of canavanine after 12 hr of incubation did not show any inhibitory effect on the enzyme activity. However, the earlier the time of canavanine addition is, the lower the amylase activity is. These results indicate that inhibition cf GAS induced amylase activity by canavanine is due to aff action of canavanine on certain procedure of transcription including mRNA metabolism rather than on translation of amylase protein itself.
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Canavanine 이 보리종자(種字)에서 $GA_3$에 의해 유도(誘導)되는 Amylase 활성증가(活性增加)에 미치는 영향(影響)
박인호,권영명,Park, In-Ho,Kwon, Young-Myung 생화학분자생물학회 1981 한국생화학회지 Vol.14 No.3
보리(Hordeum, vulgare L.cv. Baecdong) 종자(種子)에서 $GA_3$에 의(依)해 유도(誘導)되는 amylase 활성(活性)에 대(對)한 L-canavanine의 효과를 조사 하였다. Canavanine은 2 mM에서 $GA_3$에 의한 amylase 활성 증가(增加)를 완전(完全)히 조사(阻害)하였고 $GA_3$에 의해 유도되는 amylase isozyme의 전기영동석 pattern에도 영향을 주었으며 이러한 조사(助害) 효과(效果)는 arginine에 의해 감소 되었다. 또 $GA_3$ 처리 후 canavanine 첨가 시간을 달리하여 24시간 배양했을 때 amylase 활성이 3시간 후에 처리한 구(區)와 6시간 후에 처리한 구(區)에서 현저한 차이(差異)가 났으며, 12시간 이후에 첨가한 경우에는 억제작용이 나타나지 않았다. 이와같은 사실은 보리 종자에서 $GA_3$에 의해 유도되는 amylase 활성 증가에 대한 canavanine의 저해작용은 amylase 단백질 자체의 합성 단계 보다는 mRNA 대사를 포함하는 $GA_3$ 처리 12시간 이전의 어떤 단계(transcriptional stage)에서 canavanine이 저해 작용을 나타내는 것으로 추정할 수 있겠다. The effect of L-canavanine on the $GA_3$-induced amylase activity was studied in barley half seed (Hordeum vulgare L. cv. Baecdong). Canavanine, 2 mM, inhibited completely the activity of amylase and the content of soluble protein induced by $GA_3$. And 2 electrophoretic isozyme binds also disappeared. This inhibition was recovered by the addition of arginine. Inhibitory effect of canavanine on the enzyme activity was altered according to different time of the addition during 24 hr of incubation of the half seeds with $GA_3$. The addition of canavanine after 12 hr of incubation did not show any inhibitory effect on the enzyme activity. However, the earlier the time of canavanine addition is, the lower the amylase activity is. These results indicate that inhibition of GAS induced amylase activity by canavanine is due to affection of canavanine on certain procedure of transcription including mRNA metabolism rather than on translation of amylase protein itself.
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암처리한 인삼엽의 Greening 과정에서의 Chlorophyll - protein Complex 형성에 대한 연구
박인호,홍영남,권영명,이순희,조영동 ( In Ho Park,Young Nam Hong,Young Myung Kwon,Soon Hee Lee,Yong Dong Cho ) 생화학분자생물학회 1982 BMB Reports Vol.15 No.1
The formation and chlorophyll composition of chlorophyll-protein complexes were investigated in the etiolated ginseng leaves during greening under continuous white light(2000 lux, 25℃, 66% relative humidity). Six chlorophyll-containing bands (CP1a, CP1, LHCP1, LHCP2, LHCP3, free pigment zone) were separated by SDSPAGE from ginseng leaves(grown under light). Chlorophyll a-rich CP1 complex and chlorophyll a/b-rich LHCP complexes reveal their maximum absorption peaks at 676 ㎚ and 670 ㎚, respectively. And chlorophyll a/b-rich LHCP complexes have another minor peak at 652nm. CPI and LHCP complexes have already formed in the etiolated ginseng leaves after 12 hr light treatment. And chlorophyll a/b ratio of CP1 complex and the percentages of chlorophyll contents of each complexes increased progressively during greening. These results suggest that the light-induced biosynthesis of CP complex molecules and the formation of functional PSI and PS II could occur within 12 hr of illumination in the etiolated ginseng leaves during greening.
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암처리한 인삼엽의 Greening 과정에서의 Chlorophyll-protein Complex 형성에 대한 연구
박인호,홍영남,권영명,이순희,조영동,Park, In-Ho,Hong, Young-Nam,Kwon, Young-Myung,Lee, Soon-Hee,Cho, Yong-Dong 생화학분자생물학회 1982 한국생화학회지 Vol.15 No.1
암처리(暗處理)한 인삼엽(人蔘葉)을 greening시키는 과정에서(광도 ; 2,000 lux연속광 ; 온도 $25^{\circ}C$;상대습도, 66%) 형성되는 chlorophyll-protein complex의 전기영동 패턴, 각(各) complex의 엽록소조성 및 흡수스펙트럼 양상을 조사하였다. 광하(光下)에서 자란 인삼엽(人蔘葉)의 thylakoid membrane을 5% SDS-PAGE로 분리하였을 때 6개의 chlorophyll-containing band를 볼 수 있었다(CP1a, CP1, LHCP1, LHCP2, LHCP3 complex, free pigment zone). Chlorophyll a-rich CP1 complex와 chlorophyll a/b-rich LHCP complex는 676nm와 670nm에서 각각 흡수극대를 나타냈으며, chl. a/b-rich LHCP complex는 652nm에서 minor한 peak를 나타내었다. 암처리 인삼의 greening과정에서 CP1 LHCP complex는 12시간의 광(光)처리후에 이미 형성되어 있었으며, CP1 complex의 chl. a/b ratio와 각 complex의 chlorophyll함량은 greening이 진행됨에 따라 점차 증가하는 경향을 보이고 있다. 이러한 결과는 광(光)에 의해 유도되는 CP complex분자의 생합성 및 기능적으로 활성을 갖는 PSI과 PSII의 형성이 광(光)처리 12시간내에 나타난 가능성을 시사한다. The formation and chlorophyll composition of chlorophyll-protein complexes were investigated in the etiolated ginseng leaves during greening under continuous white light(2000 lux, $25^{\circ}C$, 66% relative humidity). Six chlorophyll-containing bands (CP1a, CP1, LHCP1, LHCP2, LHCP3, free pigment zone) were separated by SDSPAGE from ginseng leaves(grown under light). Chlorophyll a-rich CP1 complex and chlorophyll a/b-rich LHCP complexes reveal their maximum absorption peaks at 676nm and 670nm, respectively. And chlorophyll a/b-rich LHCP complexes have another minor peak at 652nm. CPI and LHCP complexes have already formed in the etiolated ginseng leaves after 12 hr light treatment. And chlorophyll a/b ratio of CP1 complex and the percentages of chlorophyll contents of each complexes increased progressively during greening. These results suggest that the light-induced biosynthesis of CP complex molecules and the formation of functional PSI and PS II could occur within 12 hr of illumination in the etiolated ginseng leaves during greening.
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분리한 배추 엽록체의 엽록소 형광에 미치는 구리이온의 영향
박인호,권영명 생화학분자생물학회 1991 BMB Reports Vol.19 No.3
Copper inhibited chlorophyll fluorescence intensity at room temperature in isolated Chinese cabbage chloroplasts, and copper treatment induced pigment degradation. Fluorescence inhibition and pigment degradation by copper treatment was higher at acidic condition than at alkaline condition. Light illumination after copper treatment increased the copper toxicity upon the pigment content and fluorescence intensity. The pH dependency of copper toxicity on fluorescence and pigment degradation was different from the pH dependency of that on PS-2 mediated O₂ evolution. These results suggest that copper inhibition on fluorescence intensity may be due to the conformational changes of thylakoids.
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