마크로라이드-린코사마이드-스트렙토그라민 B(MLS)계 항생물질에 대한 유도 내성

권애란(Ae Ran Kwon),최성숙(Sung Sook Choi),김숙경(Sook Kyung Kim),정영자(Young Ja Chung),최응칠(Eung Chil Choi),김병각(Byoung Kak Kim) 대한약학회 1994 약학회지 Vol.38 No.3

Forty nine clinical isolates of S. aureus showing resistance to erythromycin(EM) were selected from 83 strains isolated recently in Korea. Fourteen strains of S. aureus showing inducible resistance to MLS antibiotics were selected by disc agar diffusion method. Colony hydridization was executed using two MLS inducible resistance genes, ermA and ermC, identified previously from S. aureus as probes. S. aureus 375 and S. aureus 507 whose genes were not homologous to those probes were finally selected. It was confirmed that the resistance genes of S. aureus 375 and S. aureus 507 had no homology with those probes in southern hybridization test using ermA, ermC and ermAM as probes. It was determined that S. aureus 375 had a plasmid whose size was about 35 kb. To know if the plasmid may have the genes related to inducible resistance to MLS antibiotics, it was attempted to transform Bacillus subtillis BR151 and S. aureus RN4220 with the plasmid isolated from S. aureus 375. It was shown that the gene related to inducible resistance to MLS antibiotics did not exist in this plasmid. These results indicate that two clinical isolates of S. aureus showing inducible resistance to MLS antibiotics have novel genes that have no homology with MLS resistance genes identified so far. It is assumed that these genes may exist in chromosomal DNA.

Activities of Clindamycin, Synercid, Telithromycin, Linezolid, and Mupirocin against Gram-positive Coccal Strains Resistant to Erythromycin in Korea

권애란,Eun-Jeong Yoon,Jong-Min Yoon,Hyunjee Kim,심미자,최응칠 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.7

The antibacterial activities of clindamycin, synercid, telithromycin, linezolid and mupirocin were evaluated against erythromycin-resistant Gram-positive coccal clinical isolates collected in Korean hospitals. In Staphylococcus aureus, synercid, linezolid and mupirocin were the most active agents. Against coagulase-negative staphylococci (CNS), synercid, linezolid and mupirocin were also active. Telithromycin and synercid resistance was common against enterococci, only linezolid and mupirocin were active. The reason of low activity of telithromycin against staphylococci and enterococci is because most of the isolates were constitutively resistant to erythromycin. Synercid, telithromycin, linezolid and mupirocin were active against streptococci.

ermK Leader Peptide : Amino Acid Sequence Critical for Induction by Erythromycin

권애란,민유홍,윤은정,김정아,심미자,최응칠 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.12

The ermK gene from Bacillus lichenformis encodes an inducible rRNA methylase that confers resistance to the macrolide-lincosamide-streptogramin B antibiotics. The ermK mRNA leader sequence has a total length of 357 nucleotides and encodes a 14-amino acid leader peptide together with its ribosome binding site. The secondary structure of ermK leader mRNA and a leader peptide sequence have been reported as the elements that control expression. In this study, the contribution of specific leader peptide amino acid residues to induction of ermK was studied using the PCR-based megaprimer mutation method. ermK methylases with altered leader peptide codons were translationally fused to E. coli β-galactosidase reporter gene. The deletion of the codons for Thr-2 through Ser-4 reduced inducibility by erythromycin, whereas that for Thr-2 and His-3 was not. The replacement of the individual codons for Ser-4, Met-5 and Arg-6 with termination codon led to loss of inducibility, but stop mutation of codon Phe-9 restored inducibility by erythromycin. Collectively, these findings suggest that the codons for residue 4, 5 and 6 comprise the critical region for induction. The stop mutation at Leu-7 expressed constitutively ermK gene. Thus, ribosome stalling at codon 7 appears to be important for ermK induction.

장애인의 사회적 배제와 삶의 질에 대한 직무만족의 조절효과

권애란,이형기 한국사물인터넷학회 2024 한국사물인터넷학회 논문지 Vol.10 No.1

본 연구의 목적은 장애인의 사회적 배제 정도를 객관적으로 수치화하여 도출된 사회적 배제 정도가 장애인의삶의 질에 미치는 영향과 직무만족이 장애인의 사회적 배제와 삶의 질에 미치는 영향을 조절하는지를 검증한 것이다. 연구대상은 장애인 고용 패널조사를 통하여 추출된 1,280명을 대상으로 하였다. 독립변수인 사회적 배제는 경제적 어려움, 고용상태, 정신적 건강, 사회활동이다. 종속변수는 삶의 질 그리고 조절변수는 직무만족으로 하였다. 이 연구 결과는 다음과 같다. 첫째, 삶의 질에 영향을 미치는 사회적 배제의 하위요인은 경제적 어려움, 정신적 건강, 사회활동, 고용상태로 나타났다. 둘째, 사회적 배제와 삶의 질의 영향 관계에서 직무만족은 조절효과가 나타났다. 그리고 삶의 질에조절효과가 있는 사회적 배제의 하위요인은 경제적 어려움, 고용상태, 사회활동으로 확인되었다. 후속 연구는 선진국의장애인고용프로그램을 조사하여 시행 배경, 시행내용, 성과, 한계 등을 분석하여 장애인 고용정책의 수립에 기여해야할 것이다. 이러한 연구를 통하여 장애인의 사회적 배제와 차별의 악순환이 개선되고 장애인의 경제활동을 통하여 삶의질이 향상되는 계기가 마련되길 기대한다. The purpose of this study is to objectively quantify the degree of social exclusion of the disabled and verify the impact of the derived degree of social exclusion on the quality of life of the disabled. And the purpose is to verify whether job satisfaction moderates the impact of social exclusion and quality of life of disabled people. The subjects of the study were 1,280 people extracted through a panel survey on employment of the disabled. The independent variables of social exclusion are economic difficulties, employment status, mental health, and social activities. The dependent variable was quality of life, and the control variable was job satisfaction. The research results are as follows. First, the sub-factors of social exclusion that affect quality of life were economic difficulties, mental health, participation in social activities, and employment status. Second, job satisfaction showed a moderating effect in the relationship between social exclusion and quality of life. And the sub-factors of social exclusion that have a moderating effect on quality of life were identified as economic difficulties, employment status, and social activities. In follow-up research, it will be necessary to investigate employment programs for the disabled in developed countries and analyze the implementation background, implementation details, achievements, and limitations, thereby contributing to the establishment of employment policies for the disabled. It is hoped that through this research, the vicious cycle of social exclusion and discrimination of the disabled will be improved and an opportunity will be provided to improve the quality of life through the economic activities of the disabled.

수생식물을 이용한 수질정화에 관한 연구

권애랑(Ae Rang Kwon),박철휘(Chul Hwi Park) 大韓環境工學會 2003 대한환경공학회지 Vol.25 No.4

주의력 문제를 호소하는 아동, 청소년의 K-WISC-Ⅳ 및 ATA의 특성

권애란 ( Ae Ran Kwon ),홍창희 ( Chang Hee Hong ) 한국정서·행동장애아교육학회(구 한국정서학습장애아교육학회) 2015 정서ㆍ행동장애연구 Vol.31 No.2

주의력 문제를 호소하는 아동, 청소년들을 대상으로 최근 개발된 K-WISC-Ⅳ 및 주의력 검사를 통해 지능과 시·청각 주의력의 관련성을 알아보았다. 90명의 아동, 청소년들에게 K-WISC-Ⅳ와 정밀주의력검사(ATA: Advanced Test of Attention)를 실시하였다. 먼저 K-WISC-Ⅳ와 ATA의 상관관계를 알아보았고, 단계적 회귀분석을 통해 전체 지능과 하위 지표를 잘 설명하는 ATA의 측정치들을 살펴보았으며, 끝으 로 지능 수준에 따른 주의력의 차이를 검증하였다. 상관분석 결과, K-WISC-Ⅳ에서 지각추론, 작업기억, 처리속도 및 전체지능이 ATA의 누락, 오경보, 반응시간 표준편 차 측정치들과 부적 상관을 보였다. 전반적인 상관패턴은 K-WISC-Ⅲ를 사용한 선 행연구들과 일치하였으나, K-WISC-Ⅳ의 4요인 분류 및 개정된 소검사 등으로 인해 언어이해 지표 등에서 선행연구와 차이를 보였다. 또한, 시각 주의력에 비해 청각 주 의력이 지능의 하위 지표들과 상관이 높았다. 단계적 회귀분석에서도 청각 주의력이 전체지능, 작업기억 및 처리속도 지표를 잘 설명하였다. 지능 수준에 따른 주의력의 차이를 보면, 시각 주의력의 누락과 청각 주의력의 오경보 측정치가 지능 수준에 가 장 민감하였다. 시·청각 주의력 검사 모두에서 반응시간 측정치는 지능 수준에 따 른 차이를 보이지 않아 지능의 영향을 가장 적게 받는 것으로 여겨진다. 본 연구의 결과는 교육적, 임상적 평가나 개입시 기초 자료로 활용될 것으로 기대된다. This study was to evaluate the relation between intelligence and attention using K-WISC-Ⅳ and ATA(Advanced Test of Attention). A total of 90 children, adolescents were assessed using the ATA and K-WISC-Ⅳ. They were verified by many statistical methods such as K-WISC-Ⅳ and ATA correlation, stepwise regression analysis and the F test difference of attention in accordance with level of intelligence. As a results, the variables of ATA were shown the negative correlations with Perceptual Reasoning Index, Working Memory Index, Processing Speed Index and Full Scale IQ except for Verbal Comprehension Index in K-WISC-Ⅳ. There were shown the results consistent with the previous studies with K-WISC-Ⅲ, but partial results did not match. It seems to be due K-WISC-Ⅳ classification of the 4 factors and revised subtests. In addition, auditory attention was a significant predictor of the FSIQ, WMI and PSI in K-WISC-Ⅳ, compared to visual attention. According to the levels of intelligence, commission error in auditory attention and ommission error in visual attention were different. Response time in visual-auditory attention were not influenced on intelligence levels. It is expected this study can be applied to educational and clinical assessment and intervention.

아동기 신체학대와 자살의 관계에서 PTSD 증상, 우울 및 사회적 지지의 영향

권애란,홍창희 한국상담심리학회 2022 한국심리학회지 상담 및 심리치료 Vol.34 No.3

The study presented an integrated model that includes childhood physical abuse, PTSD symptoms, depression, suicidal ideation and social support, and examined the relationships between the variables. Two hundred and twenty two healthy individuals (80 males and 142 females) completed the Childhood Trauma Questionnaire, Scale for Suicidal Ideation, PTSD checklist-5, Hospital Anxiety-Depression Scale and DUKE-UNC Functional Social Support Questionnaire. To test the hypothesized model, this study examined the multiple mediation effect, moderation effect and moderated mediation effect using the structural equation model. The results showed that PTSD symptoms and depression partially mediated the relationship between childhood physical abuse and suicidal ideation. Futhermore, the moderation effect of the social support was significant in the relationship between childhood physical abuse and PTSD symptoms. Finally, the moderated mediation effect of social support was significant in the relationship among childhood physical abuse, PTSD symptoms, depression and suicidal ideation. To our knowledge, this study is the first attempt to examine the relationship of childhood physical abuse, PTSD symptoms, depression, and suicidal ideation. The findings highlight the importance of social support as a protective factor. However, it is difficult to establish causality in the relationships between the variables based on cross-sectional data. Providing ongoing social support and expanding positive social networks are important steps in preventing PTSD and suicide in childhood physical abuse victims. 본 연구의 목적은 아동기 신체학대의 심각성에 주목하여, 아동기 신체학대, PTSD 증상, 우울, 자살 및 사회적 지지가 포함된 모형을 제시하고, 관련된 변인 간의 유기적인 관계를 알아보는 데 있다. 성인 남녀 222명(남자 80명, 여자 142명)의 일반인을 대상으로 한국판 아동기 외상 질문지, 자살생각 척도, 사회적 지지 척도, 외상 후 스트레스 장애 체크리스트, 병원 불안-우울 척도를 사용하였다. 연구 가설을 검증하기 위해 구조방정식을 사용하여 이중 매개효과, 조절효과 및 조절된 매개효과를 분석하였다. 연구 결과, PTSD 증상 및 우울은 아동기 신체학대와 자살의 관계를 부분 매개하였다. 다음으로 사회적 지지는 아동기 신체학대와 PTSD 증상 간의 관계를 조절하였다. 마지막으로 사회적 지지는 아동기 신체학대, PTSD 증상, 우울 및 자살의 관계에서 조절된 매개효과를 보였다. 본 연구는 일반인의 아동기 신체학대, PTSD 증상, 우울, 자살 및 사회적 지지의 관계를 통합적으로 살펴본 최초의 시도이며, 자살과 PTSD 증상의 보호 요인으로서 사회적 지지의 중요성을 확인한 데 의의가 있다. 하지만, 횡단 자료를 바탕으로 진행되었기에 변인 간의 인과성을 확정 지을 수 없다는 제한점이 있다. 지속적인 사회적 지지를 제공하며 긍정적인 사회적 관계망을 확장하는 것은 아동기 신체학대 피해자의 PTSD 및 자살 예방에 있어 중요한 시사점이라고 할 수 있다.

연령에 따른 아동의 성역할 고정관념과 직업선택 일치도에 관한 연구

권애란,김수홍,나정원,신혜원,이소영,조선영,최향숙,한윤영 연세대학교 생활과학대학 1990 婦學 Vol.23 No.-

타인의 사회적 행동정보에 따른 아동의 회상 발달

권애란(Ae Ran Kwon) 한국아동학회 1993 兒童學會誌 Vol.14 No.1

The purpose of the present study was to examine age differences in children`s recall for information about aggression, prosociability, and social withdrawal of others and to investigate the impact of likeability on children`s recall for social behavioral information. The subjects were 160 children, 20 boys and 20 girls each at 6, 8, 10, and 12 years of age. The subjects listened to descriptions of the social behavior of a hypothetical boy and girl and subsequently reported their recollections. These descriptions were drawn from Bukowski`s(1990) stydy, the Pupil Evaluation Inventory, and the Pittsburgh Adjustment Scales. The data were analyzed with 2-way ANOVA, paired T-test, and correlation. Differences across the age levels were found in the number of behaviors correctly recalled for the behavioral descriptions of aggression, prosociability, and social withdrawal. Descriptions of aggressive behavior was recalled more when children listened to a hypothetical boy than to a hypothetical girl. Additional analyses revealed that at 6 years of age, children recalled more descriptions of aggressive and prosocial behavior than of withdrawal and at 10 and 12 years of age, children recalled more descriptions of withdrawal behavior than of aggressive and prosocial behaviors. The more negative the children`s affective evaluations of the withdrawal behavior, the better they recalled the withdrawal behavior.

Role of Disulfide Bond of Arylsulfate Sulfotransferase in the Catalytic Activity

권애란,최응칠 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.5

Bacterial arylsulfate sulfotransferase (ASST) catalyzes the transfer of sulfate group from a phenyl sulfate ester to a phenolic acceptor. The promoter region and the transcription start sites of Enterobacter amnigenus astA have been determined by primer extension analysis. Northern blot analysis resolved two mRNA species with lengths of 3.3 and 2.0 kb, which correspond to the distances between the transcriptional initiation sites and the two inverted repeat sequences (IRSs). By length, the 3.3 kb RNA could comprise the three-gene (astA with dsbA and dsbB) operon. ASST has three highly conserved cysteine residues. Reducing and nonreducing SDS-PAGE and activity staining showed that disulfide bond is needed for the activity of the enzyme. To identify the cysteine residues responsible for the disulfide bond formation, a series of Cys to Ser mutants has been constructed and the enzymatic activity was measured. Based on the results, we assumed that the first cysteine (Cys349) might be involved in disulfide bond mainly with the second cysteine (Cys445) and result in active conformation.