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Escherichia coli 에서 전사가 pBR322 DNA in Escherichia coli
김지현,박지수,구현숙 ( Ji Hyun Kim,Jisu Park,Hyeon Sook Koo ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.8
The deoxyribonucleoprotein complex of plasmid pBR322 was isolated from Escherichia coli cells and was treated with micrococcal nuclease. Nuclease-resistant DNA fragments were obtained due to the binding of several histonelike proteins. The relative extents of protein binding on arbitrarily divided regions of pBR322 DNA were measured. The effects of transcripticn on the protein binding to each region of pBR322 DNA were analyzed.
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Effects of Transcription on the Histonelike Proteins Binding to pBR322 DNA in Escherichia coli
김지현,박지수,구현숙,Kim, Ji-Hyun,Park, Ji-Su,Koo, Hyeon-Sook 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.8
Escherichia coli에서 플라즈미드 pBR322 DNA와 단백질의 복합체를 분리하여, micrococcal nuclease로 처리하였다. Histone 유사단백질들의 결합에 의해 nuclease가 작용하지 못한 DNA 부분에 해당하는 DNA 조각들이 생성되었다. pBR322 DNA상에 임의로 정한 구획들에 histone 유사단백질들이 결합하는 정도를 상대적으로 측정하였다. pBR322 DNA의 각 구획에 이 단백질들이 결합하는 정도가 전사에 의해 받는 영향을 분석하였다. The deoxyribonucleoprotein complex of plasmid pBR322 was isolated from Escherichia coli cells and was treated with micrococcal nuclease. Nuclease-resistant DNA fragments were obtained due to the binding of several histonelike proteins. The relative extents of protein binding on arbitrarily divided regions of pBR322 DNA were measured. The effects of transcripticn on the protein binding to each region of pBR322 DNA were analyzed.
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국민건강영양조사 자료를 통한 천식과 비만의 상관성 연구
장규진 ( Kyu Jin Chang ),구현숙 ( Hyun Sook Koo ),이현승 ( Hyeun Seung Lee ),조영아 ( Young A Jo ) 대한천식알레르기학회 2005 천식 및 알레르기 Vol.25 No.4
Background: Many studies have suggested the association between asthma and obesity both of which are important chronic health problems and socioeconomic problems. There are few studies about the association of adults in foreign nations and the association of all ages in Korea, especially based on the representative population. Objective: Authors analyze the association between asthma and indices such as BMI (body mass index), age, gender, smoking, alcohol drinking and diet habit in Korea National Health and Nutrition Survey. Method: National Survey was done from November to December 2001 and this study enrolled 27,331 men and women above 19 years of age. A structured questionnaire, weight and height were used to collect information. Obesity is defined above 25 kg/m2 of BMI. The criterion of asthma is a diagnostic history by medical doctor. Result: The overall prevalence of asthma in this study is 1.54% (male 1.50%, female 1.57%). The prevalence is higher in elderly (≥60), smokers, obese person, someone who has low educational level and does not have current partner. The significant predictors of asthma in multiple logistic regression are BMI (female OR=1.685 (95% CI=1.047∼2.713)), age (male OR=3.250 (95% CI=0.999∼10.567), female OR=6.798 (95% CI=1.583∼29.195)), educational level (male OR=5.381 (95% CI=1.857∼15.598)) and alcohol drinking (OR=0.549 (95% CI=0.323∼0.933)). Conclusion: Obesity is a risk factor of asthma in women. Old age and low educational level are social risk factors in asthma in men. (Korean J Asthma Allergy Clin Immunol 2005;25:262-268)
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권오향(O-Hyang Kwon),구현숙(Hyun-Sook Koo),이미경(Mi Kyoung Lee),김진훈(Jin Hun Kim),장안기(An Gi Chang),장동원(Dong Won Chang) 대한사회정신의학회 2006 사회정신의학 Vol.11 No.2
목 적: 본 연구의 목적은 정신분열병 환자의 일차가족의 기질성격특성을 연령과 성별을 맞춘 일반 인구 대조군과 비교해서 알아보고자 시행되었다. 방 법: 본 연구의 대상자는 DSM-IV에 의해 정신분열병으로 진단된 환자의 일차가족 32명과 이들과 성별과 연령을 맞춘 일반 인구 대조군 32명이었다. 이들에게서 한국어판 성격기질척도를 평가하였으며, 독립변인 t 검증으로 두 군간의 점수를 비교하였다. 결 과: 두 군 간에 유의한 차이를 보이는 기질성격척도 상의 점수는 협조성이었다. 일차가족에서는 29.06±5.21로 일반대조군의 31.75±4.77에 비해서 통계적으로 유의하게 낮았으며(p=.03), 그 외의 다른 기질성격특성의 차이는 없었다. 결 론: 정신분열병 환자의 일차가족은 협조성이 유의하게 낮게 나타났으며, 이러한 점은 일차가족은 자신 위주로 생각하며 다른 이들의 감정이나 권리에는 무관심하다는 경향이 있다는 것을 의미한다. 하지만, 일부 제한점은 추후에 정신분열병 일차 가족을 대상 으로 하는 많은 대상자를 상대로 한 보다 잘 설계된 연구가 필요하다고 하겠다. Objectives:The aim of this study is to compare characteristics of temperament and character between first-degree relatives of schizophrenic patients and age- and sex-matched control probands. Methods:Schizophrenic patients were outpatients and inpatients in Seoul National Hospital diagnosed by DSM-IVTR diagnostic criterion. Their first-degree relatives(n=32) and sex- and age-matched probands(n=32) were enrolled in this study. Cloninger’s Temperament and Character Inventory(TCI) and other demographic characteristics were assessed. Results:There was a significant difference in educational level between two groups. Among character subdivisions in TCI, total score of Cooperativeness(CO) was significantly lower in first-degree relatives of schizophrenic patients than that in healthy probands(29.06±5.21 and 31.75±4.77, respectively, p=.03). There were no other differences in temperament subdivision other than CO. Conclusion:First-degree relatives of schizophrenic patients have lower CO than control groups. This result suggests that first-degree relatives of schizophrenic patients are more intolerant, insensitive, selfish and revengeful. Further well controlled study investigating the characteristics of temperament and character in first-degree relatives of schizophrenia is needed.
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