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소건중탕가 용골,모려의 항산화효과, 세포활성 및 유전자 발현에 미치는 영향
김태중 ( Tae Joong Kim ),임희용 ( Hee Yong Lim ),관민아 ( Min Ah Kwak ),변준석 ( Joon Seok Byun ),서정철 ( Jung Chul Seo ),한상원 ( Sang Won Han ) 대한본초학회 2003 大韓本草學會誌 Vol.18 No.2
N/A Objectives : The purpose of this study was to determine the effect of SG-YM on antioxidant effect, cell viability and the expression of genes in human stomach cancer cells. Methods : Antioxidant effect of SG-YM was compared with that of vitamin C on DPPH radical, respectively. Cell viability was determiend by MTT assay. Finally, gene expression was evaluated by cDNA microarray assay. Results : 1. Scavenging effect of SG-YM on DPPH treated cells were demonstrated by differential antioxidant effect in SG-YM-treated group at the dose of 10 and lmg/ml. 2. Cell viability was increased in SG-YM-treated group at the dose of lmg/ml. 3. Among 8000 unigenes, 208 genes were upregulated while 90 genes were downregulated by SG-YM treatment on human stomach cancer cell line. Conclusions : Collectively, these results can be used as basic data for development of new anticancer drugs or treatment.