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최강주(Kang Ju Choi),고성용(Sung Ryong Ko),전병선(Byeong Seon Jeon) 한국생약학회 1989 생약학회지 Vol.20 No.4
As a part of studies on the quality control of crude drug preparation (So-Shi-Ho-Tang), index components of Glycyrrhizae Radix were identified by TLC and quantified by HPLC. Specific red spot (Rf=0.47) was identifted in acid hydro lysate of glycosidic fraction on silica gel plate with benzene/ethyl acetate (1 : 1, v%v). The content of glycyrrhizin was determined by quantification of glycyrrhetinic acid by HPLC on μ-Bondapak C<sub>18<sub> column with MeOH/H₂O/HAc (78 : 19 : 3, v/v). Its recovery rate in the extract granules, compared to the content in the Glycyrrhizae Radix, was 83.3±0.7%.
인삼의 근 , 엽 및 경(莖)의 사포닌 추출과정중 지용성 용매류의 정제효과
주현규,최강주,김석창,고성용 한국농화학회 1987 Applied Biological Chemistry (Appl Biol Chem) Vol.30 No.4
This study was carried out to investigate effects of fat-soluble solvents on the purification against nan-saponin substances such as chlorophylls and other pigments and on the yields of saponins in separating saponins from ginseng root, leaf and stem. Ginseng root saponins were effectively purified by various fat-soluble solvents while ginseng leaf stem saponins were by chloroform. And alternative extractions of ethyl acetate, ethyl ether, chloroform and benzene there more effective for ginseng leaf stem saponins than that by any single solvent. Contents of crude saponin fractions and total ginsenosides in ginseng leaf were 18.5∼19.5% and 10.8∼1.4%, which were very high compared with 4.6∼5.1% and 2.0∼2.6% in ginseng root or 2.2∼2.5% and 0.63∼0.67% in ginseng stem. Therefore, ginseng leaf is good resources for total saponin or ginsenosides-Rg₁, -Re, -Rc, -Rd, -Rb₂ and -Rf.