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젖소 유방염에서 16S rRNA 파이로시퀀싱을 이용한 우유 내 마이크로바이옴의 동정과 난백의 항균효과
김단일,김은경,성원진,노영혜,고대성,김남형,김재홍,권혁준,Kim, Danil,Kim, Eun-Kyung,Seong, Won-Jin,Ro, Younghye,Ko, Dae-Sung,Kim, Nam-Hyung,Kim, Jae-Hong,Kwon, Hyuk-Joon 대한수의학회 2017 大韓獸醫學會誌 Vol.57 No.2
Bovine mastitis is an important microbial disease in the dairy industry. We investigated the frequencies of bacterial pathogens in 62 farms and pathogen antibiotic resistance from mastitis samples (n = 748). We tested the antimicrobial activity of chicken and duck egg white and lysozyme purified from chicken egg white. Moreover, we compared the microbiomes of normal and mastitic raw milk obtained by 16S rRNA gene pyrosequencing and culture methods. The results showed that the frequencies of Gram-positive pathogens (Enterococcus faecalis 37% and Staphylococcus aureus 36%) were higher than that of a Gram-negative pathogen (Escherichia coli 15%). Resistance frequencies to ampicillin and norfloxacin were lowest in Staphylococcus aureus (21%), Enterococcus faecalis (23%), and Escherichia coli (33%), and the antimicrobial activity of chicken egg white was higher than those of lysozyme and duck egg white. Pyrosequencing results revealed clear differences between the microbiomes of mastitic and normal raw milk samples and revealed a slightly similar, but clearly different, composition of pathogens compared to that from the culture method. Thus, pyrosequencing may be useful for elucidating changes in microbiomes during mastitis progression and treatment. A chicken egg white and antibiotic combination may help with mastitis treatment; however, further studies are needed.
rpoB 염기서열 분석을 이용한 응고효소 음성 포도알세균 분자 동정
성원진,김단일,김은경,고대성,노영혜,김재홍,권혁준,Seong, Won-Jin,Kim, Danil,Kim, Eun-Kyung,Ko, Dae-Sung,Ro, Younghye,Kim, Jae-Hong,Kwon, Hyuk-Joon 대한수의학회 2018 大韓獸醫學會誌 Vol.58 No.1
Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.
HDTV SMPS Aging System을 위한 원격 계측 제어 시스템 개발
김병욱(Byoung-Wook Kim),임승관(Seung-Kwan Lim),고대성(Dae-Sung Ko),노신래(Shin-Rea Roh),김영민(Yeong-Min Kim) 대한전기학회 2009 전기학회논문지 P Vol.58 No.1
In this paper, A telemetering control system for the HDTV SMPS Aging System is developed. Manual Aging System is usually used to measurement of a produced SMPS. But in this system, the respective operator is arranged and monitoring the test cycle visually. According to an excess of the test time and mistakes of an operator, the reliability makes come to be low. Therefore as using the telemetering control system, it improves a productivity and a reliability of the HDTV SMPS. In the developed system, electrical signal is measured from aging system, and converted into the digital signal. Also the telemetering control system is composed of many units - telemetering control units for collecting the data, data relay units for the digital transmission service, data division for the multichannel realization.