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RISS 인기검색어
- 검색키워드
- 저자 : 강현삼 ( Seung Taek Lee (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
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A Computer Program Searching for tRNA Gene from Base Sequences of DNA
이승택,김준형,강현삼,Lee, Seung-Taek,Kim, Jun-Hyung,Kang, Hyen-Sam 생화학분자생물학회 1984 한국생화학회지 Vol.17 No.3
유전자 재조합 기술과 유전자의 염기서열 결정 방법의 진보에 의한 많은 정보의 축적은 유전자 구성과 발현의 연구를 위한 보다 신속한 염기서열분석을 요구하게 되었다. 일차로, DNA 염기서열(일차구조)로부터 tRNA 유전자를 찾는 computer program을 개발하였다. 이 computer program은 tRNA 유전자가 clover 잎 모양의 이차구초를 내포하고 있는 성질을 이용하였으며, intervening sequence(IVS)의 존재유무에 관계 없이 작동하도록 고안되었다. 이 program은 IVS가 존재가능한 yeast의 DNA 염기서열로부터 tRNA 유전자를 성공적으로 찾아내었다. A dramatic increase of data produced by simpler methods of nucleic acid sequencing has needed the fast analysis of sequence data in research of gene organization. A computer program was developed for search of tRNA genes from linear DNA sequences. This program was based on the property that the DNA sequences reflect the tRNA secondary structure that has the clover leaf shape. And this program was designed to be able to work where an intervening sequence (IVS) mayor may not be present. The program has been successfully applied to tRNA genes of yeast where an IVS may or may not be present.
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DNA 염기서열부터 tRNA 유전자를 찻아내는 Computer program 의 개발
이승택,김준형,강현삼 ( Seung Taek Lee,Jun Hyung Kim,Hyen Sam Kang ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.3
A dramatic increase of data produced by simpler methods of nucleic acid sequencing has needed the fast analysis of sequence data in research of gene organization. A computer program was developed for search of tRNA genes from linear DNA sequences. This program was based on the property that the DNA sequences reflect the tRNA secondary structure that has the clover leaf shape. And this program was designed to be able to work where an intervening sequence (IVS) may or may not be present. The program has been successfully applied to tRNA genes of yeast where an IVS may or may not be present.
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김영준,이승택,이병재,강현삼,Kim, Young-Joon,Lee, Seung-Taek,Lee, Byeong-Jae,Kang, Hyen-Sam 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.2
A single clone harboring $tRNA^{Asp}$ gene was isolated by colony hybridization to the total tRN A gene clone of Aspergillus nidulans using the previously sequenced $tRNA^{Asp}$ probe. The gene was localized and sequenced by the method of Maxam and Gilbert. The sequence analysis revealed that the $tRNA^{Asp}$ gene has the anticodon of GUC, and contains an intervening sequence of 15 bases. The nucleotide sequence of this $tRNA^{Asp}$ gene was compared with $tRNA^{Asp}$'s of the other organisms. From this, some structural features in the flanking sequences and the gene were discussed. $tRNA^{Asp}$을 [r-$^{32}P$] ATP로 표지하여 Aspergillus nidulans 총 tRNA 유전자은행 (gene bank)과 colony hybridization 한 결과, pANt12가 양성반응을 나타내었다. pANt12 DNA를 추출하여 제한효소 지도를 작성하고 Southern hybridization하여 $tRNA^{Asp}$ 유전자를 포함하는 부분은 pASPI과 pASPII으로 두 번의 subcloning을 하고 유전자의 위치를 hybridization 방법으로 결정하였다. Maxam과 Gilbert의 방법으로 유전자의 염기순서을 결정하니 앞의 $tRNA^{Asp}$의 RNA 염기순서와 IVS 부분을 제외하고는 일치함을 보였다. $tRNA^{Asp}$ 유전자에는 다른 것과는 달리 15bp의 IVS 가 존재하는 것으로 나타났고 내부조절 부위에 대한 공통 염기순서, 가능한 ACT-TA box와 5'-측쇄부위의 TATTTT염기순서가 나타났다. tRNA 구조 유전자의 일부 염기순서와 같은 염기순서가 -15 부위에서 나타났으며 3'-측쇄부분에 short, discontinuous T-strech type이 존재하였다.
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김영준,강현삼,이병재,이승택 생화학분자생물학회 1991 BMB Reports Vol.19 No.2
A single clone harboring tRNA^(AsP) gene was isolated by colony hybridization to the total tRNA gene clone of Aspergillus nidulans using the previously sequenced tRNA^(AsP)probe. The gene was localized and sequenced by the method of Maxam and Gilbert. The sequence analysis revealed that the tRNA^(AsP) gene has the anticodon of GUC, and contains an intervening sequence of 15 bases. The nucleotide sequence of this tRNA^(AsP) gene was compared with tRNA^(AsP')s of the other organisms. From this, some structural features in the flanking sequences and the gene were discussed.
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