해수에 잠긴 인공기질 표면에 먼저 부착된 박테리아가 규조류의 부착에 미치는 영향

강정훈,조병철,심재형,최중기,Kang, Jung-Hoon,Cho, Byung-Cheol,Shim, Jae-Hyung,Choi, Joong-Ki 한국해양학회 1998 바다 Vol.3 No.4

해수에 잠긴 인공기질 표면에 부착한 박테리아가 규조류의 후속부착에 미치는 영향에 대해 상반된 견해가 있다. 그러나 자연조건의 해수에서 인공기질 표면에 먼저 부착한 해산 박테리아가 규조류의 후속부착에 어떤 영향을 주는지에 대해서는 아직 알려져 있지 않다. 따라서 본 연구에서는 해산 박테리아를 일정 시간별로 미리 부착시킨 여러 재질을 이용하여 실험하였다. 첫번째 실험에서는 유리 슬라이드에 미리 해산 박테리아를 0~133 시간별로 부착시키고 해수에 일정기간 노출시킨 후 규조류의 부착수도를 조사하였다. 규조류의 부착은 먼저 부착된 박테리아의 수도와 관련이 없었으며(p>0.05), 무처리 슬라이드에 가장 많은 수의 규조류가, 가장 높은 수도의 박테리아가 부착된 표면에는 가장 적은 수의 규조류가 부착하였다. 두번째 실험에서는 유리와 아크릴 슬라이드를 이용하여 해산 박테리아를 6 일 동안 먼저 부착시킨 것과 한천(agar)을 입힌 슬라이드 그리고 대조구로서 무처리 슬라이드를 해수 중에 함께 노출시켰다. 박테리아가 가장 높은 수도($5.4{\pm}0.02{\times}10^5\;cells\;cm^{-2}$)로 부착된 아크릴 슬라이드에서는 무처리 슬라이드보다 적은 수의 규조류가 부착하였으나, 박테리아가 $2.5{\pm}1.0{\times}10^5\;cells\;cm^{-2}$로 부착된 유리 슬라이드에서는 무처리 슬라이드와 차이가 없었다. 반면 점액질을 모사한 표면에 부착한 규조류의 수도와 이입계수는 다른 표면들에 비해 평균 2 배 이상 높게 나타났다. 따라서 해산 박테리아의 부착이 규조류 후속부착의 선결요건이 아닌 것으로 판단된다. Controversial observations on the effect of pre-attached bacteria on the attachment of diatoms to artificial surfaces immersed in seawater have been made. Furthermore, it is not known whether or not pre-attached bacteria on artificial surfaces influence attachment of diatoms in natural seawater. In this study, we used various surfaces to which marine bacteria were pre-attached for different incubation periods. In the first experiment, glass slides were initially attached by marine natural bacteria with different exposure time (0-133 hr) and then immersed into seawater for a certain time period. The attachment of diatoms was not affected by the abundance of pre-attached bacteria (p > 0.05). The maximum abundance of attached diatoms was found on control surfaces, and the minimum abundance on surfaces where attached bacterial abundance was highest. In the second experiment, glass slides and acryl slides, either attached by marine natural bacteria for 6 days or coated by agar, were immersed in seawater. Untreated slides were also employed. On the surfaces of acryl slides with the most abundant attached bacteria ($5.4{\pm}0.02{\times}10^5\;cells\;cm^{-2}$), abundances of attached diatoms were less than those on untreated slides. On the surfaces of glass slides with bacterial abundance of $2.5{\pm}1.0{\times}10^5\;cells\;cm^{-2}$, however, abundances of attached diatoms were not different from those of untreated slides. On the agar-coated slides, the immigration rate and immigration coefficient were on average > 2 folds compared to other surfaces, indicating high rates of diatom attachment on mucilage simulated surfaces. Therefore, it seems that pre-attachment of bacteria is not prerequisite for the attachment of diatoms on artificial surfaces.

Purification and Properties of Diamine Oxidae from Soygean(Glycine max)

강정훈,조영동,Kang, Jung-Hoon,Cho, Young-Dong 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.3

대두(Glycine max)축에 존재하는 diamine oxidase (EC 1.4.3.6)를 $(NH_4)_2SO_4$ 침전법, DEAE-cellulose, phosphocellulose, hydroxyapatite, affinity chromatography 등을 이용하여 정제하였다. 정제된 효소의 분자량은 Sephadex G-200 gel filtration에 의해 145,000으로 판명되었다. 정제된 효소는 SDS-PAGE 상에서 단일 띠를 나타냈다. 효소활성에 대한 최적 pH는 7.5였다. Putrescine은 이 효소에 대해 가장 좋은 기질이었으며 spermidine과 spermine도 기질로 작용하였다. Putrescine, spermidine, spermine에 대한 효소의 Km 값은 각각 $5.3{\times}10^{-6}M$, $2.8{\times}10^{-5}M$, $6.5{\times}10^{-5}$이었다. Diamine oxidase (EC 1.4.3.6) present in extracts of axes of soybean (Glycine max) seedling has been purified to homogeneity using series of ammonium sulfate fractionation, DEAE-cellulose, phosphocellulose, hydroxyapatite and 1,12-diaminododecane agarose column chromatography. The molecular weight estimated by a Sephadex G-200 gel filtration was about 145,000. Sodium dodecyl sulfate polyacrylamide gel electrophoresis gave a single band at the molecular weight of 70,000. The optimum pH for activity was 7.5. Putrescine was the best substrate, and spermidine and spermine also served as substrates in decreasing order. The $K_m$ values for putrescine, spermidine and spermine were $5.3{\times}10^{-4}M$, $2.8{\times}10^{-5}M$ and $6.5{\times}10^5M$, respectively.

Longitudinal Distribution of Zooplankton at 10.5^°N in the Northeastern Pacific

강정훈,조규희,손주원,김웅서,Kang, Jung-Hoon,Cho, Kyu-Hee,Son, Ju-Won,Kim, Woong-Seo Korea Institute of Ocean Science Technology 2007 Ocean and Polar Research Vol.29 No.4

We investigated the longitudinal variations in zooplankton abundances and their related physicochemical properties at nine stations located between $136^{\circ}W$ and $128^{\circ}W$ at $10.5^{\circ}N$ in the northeastern Pacific in summer 2004. Temperature, salinity, inorganic nutrients, chlorophyll-a (hereafter chl-a) and zooplankton ($>200\;{\mu}m$) were sampled within the depth from the surface to 200 m depth at $1^{\circ}$ longitude intervals. Zooplankton($>200\;{\mu}m$) samples were vertically collected at two depth intervals from surface to 200 m, consisting of surface mixed and lower layers (thermocline$\sim$200 m). Longitudinal distributional pattern of hydrological parameters (especially salinity) was physically influenced by the intensity of westward geostrophic current passage relating to the NEC (North Equatorial Current). Data from the longitudinal survey showed clear zonal distributions in the hydrological parameters(temperature, salinity and nutrients). However, spatial patterns of the chl-a concentrations and zooplankton abundances were mostly independent of the zonal distributions of hydrological parameters. The two peaks of zooplankton abundance in the surface mixed layer were characterized by different controlling factors such as bottom-up control from nutrients to zooplankton ($129^{\circ}W$) and accumulation by increment of friction force and taxonomic interrelationship ($133^{\circ}$ and $134^{\circ}W$). Divergence-related upwelling caused introduction of nutrients into surface waters leading to the increment of chl-a concentration and zooplankton abundances ($129^{\circ}W$). Increased friction force in relation to reduced flow rates of geostrophic currents caused accumulation of zooplankton drifting from eastern stations of study area($133^{\circ}$ and $134^{\circ}W$). Besides, high correlation between immature copepods and carnivorous groups such as chaetognaths and cyclopoids also possibly contributed to the enhanced total abundance of zooplankton in the surface mixed layer (p<0.05). Zooplankton community was divided into three groups (A, B, C) which consecutively included the eastern peak of zooplankton($129^{\circ}W$), the western peak($133^{\circ}$ and $134^{\circ}W$) and high nutrient but low chl-a concentration and zooplankton abundance ($136^{\circ}W$). Moreover, Group B corresponded to the westward movement of low saline waters(<33.6 psu) from 128 to $132^{\circ}W$. In summary, longitudinal distributions of zooplankton community was characterized by three different controlling factors: bottom-up control ($129^{\circ}W$), accumulation by increased friction force and relationships among zooplankton groups ($133^{\circ}$ and $134^{\circ}W$), and mismatch between hydrological parameters and zooplankton in the high nutrient low chlorophyll area ($136^{\circ}W$) during the study period.

춘계 독도 주변해역 야광충의 먹이생물

강정훈,KANG, JUNG-HOON 한국해양학회 2020 바다 Vol.25 No.4

To understand on-site feeding traits of Noctiluca scintillans with unprecedented high abundances around Dokdo in spring, 2014, the composition and abundance of food items in the digestive vacuole of the species were analyzed. Abundances of N. scintillans ranged from 4,328~17,791 inds.m^-3 around Dokdo during the study. The incidence of preyed items in the vacuole of N. scintillans ranged by averaged 32% (24~50%) in the surface waters around Dokdo. Diverse preyed items consisted of fecal pellet, protozoans, pollen, diatoms, dinoflagellates, copepods and ichtyoplankton, while fecal pellets were dominantly fed by N. scintillans (43%) and next by protozoans (19%), pollen (18%), diatoms (7%) and dinoflagellates (4%). Fecal pellet, protozoans and pollen were relatively preferred by N. scintillans compared to diatoms, dinoflagellates and ichtyoplankton based on the incidence in the food vacuoles. Present results indicated that high abundance of N. scintillans found around Dokdo was likely associated with non-phytoplankton items rather than previously well-known diatoms as prey during the study.

컴퓨터 프로그램 복잡도와 구조도

강정훈(Jung Hoon Kang),박영석(Young Seak Park),박용진(Yong Jin Park) 한국정보과학회 1985 정보과학회논문지 Vol.12 No.2

본 논문에서는 세어 흐름 복잡도 척도(control flow complexity measures)들에 대한 주요한 특성과 문제점을 지적하고, 단일 성분 척도(single component measures)의 결점을 보완해 주는 복합척도 (hybrid measures)와 구조도(structuredness)를 제안하며 제안된 척도의 타당성을 평가한다. In this paper, we discuss major properties and weaknesses of control complexity measures, propose hybrid measures and structuredness which complement the demerits of single component measures, and evaluate the validity of the proposed measures.

풍상차분법을 이용한 winglet 부착 날개 주위의 천음속 유동장 수치해석

강정훈(Jung-Hoon Kang),노오현(O-Hyun Rho),이동호(Dong-Ho Lee) 한국항공우주학회 1993 韓國航空宇宙學會誌 Vol.21 No.1

자엽을 제거한 대두 유식물에서 Polyamine 과 Methylglyoxal bis ( guanylhydrazone ) 가 Diamine Oxidase 의 활성에 미치는 영향

강정훈(Jung Hoon Kang),조영동(Young Dong Cho) 한국식물학회 1991 Journal of Plant Biology Vol.34 No.1

The effect of polyamines and methylglyoxal bis(guanylhydrazone)(NGBG) on the activity of diamine oxidase was studied in soybean (Glycine max) seedlings. 10^-2 M of putrescine, spermidine, and spermine inhibited diamine oxidase activity, whereas 10^-6 M putrescine increased enzyme activity. These results suggest that diamine oxidase can be induced by a specific subastrate, putrescine. The content of putrescine was increased in response to the increase in concentratioin of MGBG. In vitro, 40% of the diamine oxidase activity was inhibited by 10^-3 M MGBG. In vivo, the diamine oxidase activity was increased by a low concentrration of MGBG. It was suggested that MGBG inhibited the formation of spermidine and that the accumulated putrescine induced diamine oxidase, whereas the diamine oxidase acitivity was inhibited by a high concentration of MGBG. It is suggested that a high cocentration of MGBG increases the putrescine content by inhibiting diamine oxidase activity which is responsible for putrescine degradation.

재활 치료 환경의 개선을 위한 근전도 시스템 구현 및 어플리케이션

강정훈(Jung-Hoon Kang),김주영(Ju-Young Kim),박재현(Jae-Hyun Park),김진수(Jin-Su Kim),김강수(Kang-Soo Kim),문치웅(Chi-Woong Mun),민홍기(Hong-Ki Min),최홍호(Heung-Ho Choi) 대한전자공학회 2010 대한전자공학회 학술대회 Vol.2010 No.6

Many studies on electromyogram (EMG) system have been reported for the sake of persons with disabilities (PWDs) in rehabilitation training. Previously, almost all rehabilitation treatments made PWDs feel somewhat uncomfortable, which prompted them to consider these treatments repulsive. Therefore, this study provides some useful applications of EMG signals. The application module The developed GUI has display and save functions. The graph on the GUI windows assists the rehabilitation treatment by showing the condition of the muscles. The data saved from the GUI shows the conditions of the muscles of the PWDs and helps in the latter’s training. Because the application module provides visual effects, patients could relieve boredom, when participate in their treatment. In this paper, it is considered that the proposed systems can improve the rehabilitation treatment environment by using the EMG system and applications.

버섯균사체 발효 뽕잎 추출물의 항산화 활성

강정훈(Jung Hoon Kang) 한국유화학회 2017 한국응용과학기술학회지 Vol.34 No.4

본 연구에서는 뽕잎(Morus alba)을 노루궁뎅이 버섯균사체(Hericium erinaceum)로 발효시킨 열수 추출물(MA-HE)의 항산화 활성을 확인하고자 하였다. MA-HE의 항산화 활성을 DPPH radical, ABTS radical 소거활성 측정을 통해 알아보았다. 그 결과 DPPH radical 소거활성은 500 μg/mL 농도 에서 61.73%, ABTS radical 소거활성은 250 μg/mL 농도에서 97.39%로 나타났다. MA-HE은 DNA 의 산화적 손상을 효과적으로 억제하였다. 또한 생체고분자물질인 사람의 혈청단백질의 산화적 손상을 억제하였다. 세포에 H 2 O 2 를 처리하였을 때 세포생존율에 비하여 발효물을 100 μg/mL 농도로 전 처리한 세포생존율은 8% 증가했으며, 발효물을 50 μg/mL 농도로 처리했을 경우 세포 내 활성산소의 축적이 유의적으로 감소되었다. 본 연구 결과는 MA-HE이 항산화 활성을 가지고 있으며 산화적 스트레스에 의해 야기되는 세포 독성에 대한 보호 작용도 뛰어난 것으로 사료되었다. In this study, the hot water extract from Mulberry (Morus alba) Leaves fermented with Hericium erinaceum mycelium (MA-HE) was assessed for antioxidant activity. Radical scavenging activity of MA-HE evaluated using 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical and 2,2 -azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)(ABTS) radical. MA-HE showed 63% DPPH radical scavenging activity at 500 μg/mL and 98.27% ABTS radical scavenging activity at 250 μ g/mL. MA-HE was shown to significantly inhibited DNA strand breakage induced by free radical. MA-HE also inhibited free radical-mediated human serum albumin modification. MA-HE effectively inhibited H 2 O 2 induced cell death and significantly increased of the 8% cell survival at 100 μg/mL. MA-HE decreased intracellular reactive oxygen species (ROS) levels in H 2 O 2 -treated cells. The results suggested that MA-HE can contribute to antioxidant and protected cells from oxidative stress-induced cell injury.

풍상차분법을 이용한 3차원 날개 주위의 천음속 유동장 해석

강정훈(Jung-Hoon Kang),노오현(O-Hyun Rho),이동호(Dong-Ho Lee) 한국항공우주학회 1991 韓國航空宇宙學會誌 Vol.19 No.4