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Microfluidic Single-cell Trapping and Cultivation for the Analysis of Host-viral Interactions
레야 강굴리,이병진,강소립,김용식,정성근,김재성,박소영,Yamauchi Yohei,이창수 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.2
The isolation of single cells and their further cultivation in confined chambers are essential to the collection of statistically reliable temporal information in cell-based biological experiments. In this work, we present a hydrodynamic single-cell trapping and culturing platform that facilitates biological analysis and experimentation of virus infection into host cells. To find the optimum design of the cell trap at the microscale, we evaluated hook traps with different widths and trap intervals to obtain a high trapping efficiency of a single cell. The proposed design leverages the stochastic position of the cells as they flow into the structured microfluidic channels, where hundreds of single cells are then arrayed in nanoliter chambers for simultaneous cell-specific data collection. Optimum design is used to devise and implement a hydrodynamic celltrapping mechanism that is minimally detrimental to the cell viability and retains a high trapping efficiency (90%), with the capability of reaching high fill factors (90%) in short loading times (10 min) in a 450-trap device. Finally, we perform an analysis of host-viral interactions under the treatment of a drug concentration gradient as a proof of concept.
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2LO-9 Multiplexed digital assay in a three-dimensional paper-based microfluidic analytical device
정성근,진시형,이병진,심규락,김동영,강소립,이창수 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1
Here, we demonstrate 3-dimensional (3D) paper-based microfluidic channels to control 3D flow for automatic multiplexed digital assay. The 3D channels were fabricated by double-sided printing and lamination process. We print and laminate asymmetrical and symmetrical wax patterns on the paper to form 3D channels, and to create 3D fluidic flow for multiplexed digital assay. The 3D channels contains lateral and vertical channel. The asymmetrical wax-patterns form lateral and vertical channels. The symmetrical wax-patterns form vertical channels when the melted wax make contact with each other. We injected red dye into inlet of channels to track 3D capillary flow in the 3D channel. We conformed that the 3D fluid flow in a thickness of a paper. Based on this concept, we created 3D devices for automatic digital assay to detect glucose and albumin. Therefore, we expect that this method could be an instrument-free and multiplexed assay format for use in developing countries.
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