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VAE 에멀션과 수계수지 바인더의 프리코팅층 적용이 도공지 물성에 미치는 영향
김선구,유성종,김용식,이용규 한국펄프·종이공학회 2023 펄프.종이기술 Vol.55 No.1
When binders with different physical properties were applied to the pre-coat layer, coating layers having different properties were formed. This was because the characteristics of the top-coating vary depending on the top-coating layer. In particular, the properties of this top-coating layer affected the final printability. Vinyl acetate ethylene (VAE) emulsion binders offer advantages as an alternative resource because natural gas provides the base substance for polymer production. The Tg of VAE emulsion binders can be easily adjusted, so the desired physical properties can be adjusted. Therefore, in this study, SB-latex and VAE emulsion binders with different Tg were applied to the pre-coating layer. In addition, poly vinyl acohol (PVA) was applied as a co-binder, and the printability was evaluated by observing changes in physical properties of the pre-coating layer and the top-coating layer. As a result, as the Tg increased, the surface roughness of the coated paper increased while the paper gloss decreased. In addition, when PVA was blended, a tendency simillar to paper gloss was showed. Therefore, It is considered that this is because the voids formed by the pigment and the binder form different structures. .
정자 미세주입에 의한 생쥐 난포난의 체외수정과 발달 : 미세 주입정자수의 효과 The effect of microinjected sperm numbers
조병욱,신택순,이길왕,강한석,김선구 密陽産業大學校 農業技術開發硏究所 1999 農業技術開發硏究所報 Vol.3 No.2
정자의 미세주입이 생쥐 난자의 체외수정과 배발달에 미치는 영향을 조사하기 위하여 수정능 획득 후 첨체반응시킨 정자를 1~3개씩 무작위로 난자의 위난강내 주입하였다. 난자는 PMSG 와 HCG로 과배란 시킨 생쥐의 난관에서 채취하였고 정자는 lactate와 albumin이 함유된 Ham's F-10 배양액에서 2시간 배양후 12mM 의 abc GMP와 10mM 의 imidazole 포함된 배양액에서 10분간 배양하였다. 난자의 수정율은 31.4%이었고, 2세포기 28.5%, 3~6세포기 11.9%, 8세포기 5.3%로 발달하였다. 난자에 주입된 정자수는 체외수정과 배발달에 영향을 미치지 않았다. This study was conducted to investigate the in vitro fertilization of mouse oocytes by microinjection of sperm and in vitro development of mouse embryos. Capacitated and acrosome reacted sperm were injected into the perivitelline space of a mouse oocyte. Oocytes were collected from the oviduct of the mouse treated with PMSG and HCG. Sperm were incubated for 2 hrs at 37℃ under an atmosphere of 5% CO₂in Ham's F-10 medium containing lactate and albumin for capacitation and kept for 10 minutes in culture medium containing 12mM of dbeGMP and 10mM of immidazol for acrosome reaction. Single or multiple sperm were injected into the perivitelline space of each occyte. Fertilization was recognized by presence of second polar body or two prounclei. The rat of fertilization was 31.4%. The rates of embroys developed into 2-cell, 3~6cell and 8 cell stage were 28.5%, 11.9% and 5.3% respectively. The number of sperm injected into perivitelline space of occytes did not affect in vitro fertilization of oocytes and development of embryos.