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한탄바이러스 감염 제대정맥내피세포에 대한 단핵구 매개성 손상
정상인,김기우,문언수,강응택,유석희,최철순,양용태 중앙대학교 의과대학 의과학연구소 1992 中央醫大誌 Vol.17 No.4
Studies on the pathogenesis of hemorrhagic fever with syndrome(HFRS) in patients have been seriously hindered by the abscence of appropriate animal model. The major pathologic findings in patient with HFRS are generalized vasculopathy. However, the pathogenesis of disseminated intravascular coagulation (DIC) and bleeding tendency in patients with HFRS are not well understood. It has been reported that the specific viral antigens are usually demonstrated in the endothelium of glomerular capillaries, and resulted in application of primary cultured human umbilical vein endothelial of glomerular capillaries, and resulted in application of primary cultured human umbilical vein endothelial cells (HUVEC) to study on the pathogenesis of HFRS. In this study, HUVEC were infected with Hantaan virus. The outcome Hantaan virus infection of HUVEC were assessed by flurescence activated cell scanning(FACS). The result of FACS assay indicated that 11% of the cells contained veiral antigens on 3 days postinfection. The proportion of virus-containing cells increased to 85% by the fifth days, adn 96% by the seventh, respectively. These observed no CPE development in the HUVEC cells infected with Hantaan virus and observed up to 13 days. The sensitivity of immunohistochemical method used for the viral antigens appeared similar to that of indirect immunofluorescent antibody technique. The monocyte adherence rate of infected HUVEC was much higher that than that of uninfected HUVEC, The monocyte adherence rate of infected HUVEC was increased by addition of convalescent serum of patient with HFRS. Infection with Hantaan virus HUVEC stimulated the production of superoxide radical.
살무사(Agkistrodon blomhoffi brevicaudus)뱀독의 면역학적 성상
전우규,정상인,최철순,양용태 중앙대학교 의과대학 의과학연구소 1991 中央醫大誌 Vol.16 No.3
This study was undertaken to find out main toxic components in venom of Agkistrodon b. brevicaudus and to prepare antiserum to venom. Through the experiment, venom of A. b. brevicaudus was fractionated into 7 fractions through DEAE-Sephadex A-50 and observation were made with toxic reaction of fractioned venom of A. b. brevicaudus, neutralizing effect of antiserum for highly toxic fraction and finally, comparison of neutralizing effect of antisera for fractionated venom and original crude venom. The results ara summarized follows: 1. Fractionation of A. b. brevicaudus venom by DEAE sephadex A-50 column chromatograhpy resulted in seven peaks. The amount of total protein were the highest in 4th fraction. 2. To investigate the toxicity of each fraction, hemorrhagic activity of each fraction were compared. As a result, most strong hemorrhagic activity was shown in 6th fraction. Then 4th, 7th fraction were in that order. 3. Neutralizing effect of rat antisera for 2nd, 4th and 6th fraction were compared. Most effective neutralizing(antilethal) activity was shown in antiserum for 4th fraction and neutralizing effect of antiserum for 4th fraction was similar to that of crude venom.
Propionibacterium acnes Serotype Ⅰ 및 Ⅱ의 Sarcoma 180 복수암세포의 성장억제에 대한 비특이면역 효과
강영태,정상인,최철순,양용태 중앙대학교 의과대학 의과학연구소 1987 中央醫大誌 Vol.12 No.2
Although the anti-tumor effects of nonspecific active immunization with bacterial adjuvant producys may be limited, diverse biological response modifiers(BRM) including BCG, Corynebacterium parvum and Brucella abortus have been employed to enhance the immune response through the activation of macrophages and matural killer cells and to restore the T cell function of cancer patients. Propionibacterium acnes serotypeⅡ that is a predominant resident microorganism of the skin is most similar to previous descriotions of Corynebacterium parvum. In our previous study on the quantitative bacteriology of P. acnes isolated from the skin and serology with sera obtained from healthy persons, it was found that the distribution of serotypeⅠ was much higher than that of serotypeⅡ. However, the physiological role and significance of immunological function of two serotypes as BRM's were not elucidated. In this study, nonspecific tumor immunity of the saline extracts of cell wall components and ether extractedbacilli of P.acnes serotypeⅠand Ⅱ, and Brucella aboturs against the growth of Sarcoma 180 ascites tumor cells in ICR-JCR mice was assessed. The saline extracts of cell wall components appeared to be more effective than the ether extracted bacilli to inhibit the growth of ascites tumor cells. Mean survival days of mice administered with either the saline exyracts of cell wall components of P.acnes serotypeⅠor the ether extracted intact bacilli of P. acnes serotypeⅡ were significantly extended in comparison with that of control group. These results indicated that the enhancement of nonspecific immune response by P. acnes was associated with cell wall components, but there was no significant difference between two serotypes of P. acnes in antitumor immunization aganist Sarcoma 180 ascites cells in mice.
Genus Bacillus의 병원성과 Superoxide Dismutase활성도와의 관계
김환수,정상인,양용태,최철순 중앙대학교 의과대학 의과학연구소 1991 中央醫大誌 Vol.16 No.2
This study was undertaken to examine the possibility of association between pathogenicity of Genus Bacillus and the induction of superoxide dismutase(SOD) activity. In this study, pathogenic B. anthracis ATCC 14578, vaccine strains; Army No.2 and Sterne, and other 4 strains of Bacillus species were included. The induction of SOD of Genus Bacillus was tested after cultivation by candle jar method. The results are summarized as follows: 1. Induction of total SOD activities was observed in pathogenic B. anthracis ATCC 14578 when cultured under reduced oxygen tension(candle jar method). (p>0.05) 2. Nonpathogenic strains, B. anthracis Army No. 2 and Sterne, and other 4 strains of related Bacillus species, were not observed the induction of SOD activity. 3. In the induction experiments, B. anthracis ATCC 14578 showed significant increment of Mn-SOD activity. But, other nonpathogenic strains failed to show statistically significant increase of Mn-SOD activity. These results suggest that the pathogenicity of Genus Bacillus may be associated with inducible activity of SOD.
Mycobacterium scrofulaceum에 의한 마우스 폐의 Superoxide Dismutase유도
이봉진,정상인,최철순,양용태 중앙대학교 간호과학연구소 1991 中央醫大誌 Vol.16 No.3
This study was undertaken to investigate whether the induction of superoxide dismutase in the lung was affected by infection with M. scrofulaceum. In addition, association between the plasmidbearing and induction of superoxide dismutase(SOD) were investigated with various strains of M. scrofulaceum. When M. scrofulaceum were injected to ICR-JCL mouse intravenously, type 42 CDC 1198 and type 43 Brooks strains induced superoxide dismutase in the lungs, which no significant changes in the activity of superoxide dismutase in the spleen were observed. Strains of M. scrofulaceum which possessed a large plasmid(>80 Kb)induced much oxygen metabolites in the host than the plasmidless strains. This result suggests that more virulent strains of M. scrofulaceum induces the activity of superoxide dismutase in the host to protect the injuries from the reactive oxygen species during the infection.