植物잎의 電氣的 性質에 관한 硏究

허홍욱,장민수,이경화,허만규 부산대학교 1991 자연과학논문집 Vol.51 No.-

The purpose of the present work was to show the relation between photosynthesis and electrical resistivity of plant leaves. Electrical resistivity of leaves were measured under variation of retaining rate of water, temperature, seasons and light wavelength obtained various color filters; green, blue and red, respectively. Electrical resistivity of Camellia japonica L. and Aucuba japonica T. increased as retaining rate of water decreased. Electrical resistively of Camellia japonica L., Aucuba japonica T., Tradescantia reflexa R., Mirabillis jalapa L., Glycine max M. and Fagopyrum esculentum M. decreased as temperature increased. Especially, in case of Camellia japonica L. and Aucuba japonica T., the resistivity obtained in the summer that photosynthesis is active and temperature is high was lower than other seasons. The resistivity for red light that shows active photosynthesis was the lowest, comparatively. Under the same light the resistivity also decreased as temperature increased. Electrical resistivity of Fagopyrum esculentum M. showed anomaly phenomena near 31℃.

[논문]핵 내 rDNA의 내부 전사 부위에 의한 재배 홍화 품종의 관계와 유전적 다양성

허홍욱,허만규 釜山大學校 師範大學 科學敎育硏究所 2010 科學敎育硏究報 Vol.37 No.-

핵 내 rDNA의 내부 전사 부위에 의한 재배 홍화 품종의 관계와 유전적 다양성

[논문]부산시 영도의 세 지역내 식물 집단 구조

허홍욱,이송진,최금자,허만규 부산대학교 환경문제연구소 2004 環境硏究報 Vol.22 No.-

Plant Population Structure of three areas in Young-do of Busan

메밀 재배집단의 유전적변이와 지역분화에 관한 연구

허홍욱 부산대학교 사범대학 과학교육연구소 1986 科學敎育硏究報 Vol.13 No.-

The genetic variation in the Korean populations of common buckwheat, Fagopyrum esculentum Moench, was examined for the frequency of detrimental genes and allozyme variation. The frequency of detrimental genes per gamete was estimated by performing sull-sib matings and subsequent observation of F_2 segregation. The frequency of sib lines which segregated chlorophyll-deficient mutants was 18.4% at the cotyledon stage, and 7.1% at the foliage leaf stage. Following types of abnormalities were observed in the cotyledons; yellow (3.6%), pale yellow(5.2%), pale green (6.4%), variegated (1.4%) and morphological (1.8%). Each type was also found in the foliage leaves at about half of the frequencies in the cotyledons. The frequency of mutants affecting the cotyledons and foliage leaves does not vary among the Hong-cheon, Jeon-gok and Che-ju populations. By estimating the frequency of mutant allele at each locus, it was concluded that the equilibrium frequency at each locus is very low simlar regardless of locality, and that the chlorophyll-deficient mutants are maintained by mutation-selection balance. This may be expected if the Korean populations without systematic breeding. The frequency of recessive male and female sterility mutants was also investigated by examining the segregation of such mutants in the same full-sib families, as used for the detection of chorphyll-deficient mutants. The aberage frequency of male sterility mutant was 14.1% per gamete and that of female sterility was much lower than male sterility, and it was the same among the three populations. By the sta tistical analysis, it was revealed that male and female sterilities were mostly caused by different genes, and no geographical variation was found in the Korean populations. The allozyme variation for alcohol dehydrogenase (ADH), NAD dependent malate dehydrogenase (MDH), phosphoglucomutase(PGM) and 6-phosphogluconate dehydrogenase (6-PGDH) was assayed by mans of starch gel electrophoresis. ADH was monomorphic in all of the the Korean populations examined, but several seeds were apparently heterozygous for rare mutant alleles. The MDH-1 locus was shown polymorphic and the frequency of minor fast allele was 25.5% on the aberage. However, the Dun-nae population showed a low frequency of the minor allele(14.7%). The genetic distances between the Dun-nae population and the other Korean populations are slightly larger than others reflecting the significantly lower frequency of the fast allele at the MDH-1 locus in Dun-nae. At the 6-PGDH locus, the frequency of minor slow allele is only 1.5%. Furthermore, another minor fast allele was occasionally observed in three populations, but its frequency is extremely low, ranging from 0.3 to 0.8%. For the PGM locus, all of the six populations showed triallelic polymorphism. The frequencies of fast and slow minor alleles were approximately equal, being 4.7% and 6.0%, respectively. As the result of the allozyme variation, the gene frequency of the Korean populations was similar to that of the western population of Japan. This may lead to the conclusion that the Korean populations are closely related to the western populations of Japan.

Gene diversity and population structure in Korean populations of scilla scilloides druce

허홍욱 부산대학교 사범대학 1998 교사교육연구 Vol.36 No.-

한국의 무릇 (Scilla scilloides Druce)은 백합과 식물로 Scilla속으로는 우리나라에서는 유일한 종이다. 이 무릇의 유전적 다양성과 집단구조를 밝히기 위해 전분전기영동 실험을 실시하였다. ADH를 비롯한 8개의 효소시스템으로 12집단을 분석하였다. 분석한 16 대립유전자 좌위당 11개가 다형 현상을 나타내었다. 집단 내 다양도는 0201로 나타났는데. 무릇과 유사한 생활양식을 가진 온대지역 식물, 타가수분, 다년생 초본에 비해 높았다. 조사된 유전자 좌위에서 통계적으로 중요한 대립인자의 빈도가 나타났으며, 고정지수 분석에서 하아디-바인베르그 평형에 비해 이형접합자의 결핍이 현저하였다. 또한 집단 간 분화는 단지 6%에 불과 하였다. 이는 화분과 종자분산에 의한 높은 gene flow (Nm=3.53), 높은 타가수분율 (88%), patchy 분포 등이 반영된 소산일 것이다.

ISSR을 이용한 고추나물 집단의 유전적 다양성과 계통학적 연구

허홍욱,허만규,강동호,Huh, Hong-Wook,Huh, Man-Kyu,Kang, Dong-Ho 한국생명과학회 2007 생명과학회지 Vol.17 No.6

Inter simple sequence repeat (ISSR) markers were performed in order to analyse the phylogenetic relationships of eight Hypericum electum populations in Korea. The six primers were produced 37 reproducible ISSR bands. Analysis of ISSR from individual plants of Korean H. erectum resulted in 22 polymorphic bands with 59.5%. Across populations, the mean number of alleles per locus was 1.348 and Shannon's information index was 0.203.Population Mt. Gyeryong had the highest expected genetic diversity (0.175) among all populations. When species were grouped by eight populations, within group diversity was 0.140 (Hs), while among group diversity was 0.472 (G$_{ST}$) on a per locus basis. The estimated gene flow (Nm) for H. erectum was very low (0.561). It is suggested that reproductive isolation by the isolation of geographical distance among H. electum populations and genetic drift may have played roles in shaping the population structure of this species. In phonetic tree, all populations were well separated from each other. Thus, ISSR markers are very effective in classifying natural population levels of genus Hypericum in Korea.

한국 메밀재배 집단의 Isozyme 의 분석에 관한 연구

허홍욱 한국유전학회 1984 Genes & Genomics Vol.6 No.3

N/A

과학전람회 작품 중 중학교 과학분야의 분석

허홍욱,손수옥,허만규 한국과학교육학회 2003 한국과학교육학회지 Vol.23 No.2

The study is to analyze the middle school-science parts among the products of nation-wide science exhibition for eleven years recently and the results of compared to the content of science-textbooks of middle school. The number of observation in the student parts among the products of nation-textbooks of middle school. The number of observations in the student parts among the products of nation-wide science exhibition was the highest in all methods studied, whereas, most teachers surveyed experiments in their products. The mean Shannon's diversity index (DI) was same trend for students and teachers. There was a non-significant difference of the methods in students and teacher groups according to years and the association between students and teachers were closely connected with methods. The correlation between the content of students' products and texts of middle-school was highly positive for earth science, chemistry, and biology, whereas physics showed negative correlation.

Genetic Diversity and Population Structure of Liriope platyphylla (Liliaceae) in Korea

허홍욱,최주수,이복규,허만규,Huh, Hong-Wook,Choi, Joo-Soo,Lee, Bok-Kyu,Huh, Man-Kyu Korean Society of Life Science 2007 생명과학회지 Vol.17 No.3

한국내 분포하는 맥문동(Liriope platyphylla) 11집단에 대한 20 알로자임 대립유전자좌위에서 유전적 다양성과 집단구조를 조사하였다. 효소내 다형성을 나타내는 빈도는 55.9%였다. 종과 집단 수준에서 유전적 다양도는 각각 0.178, 0.168로 높았으며, 집단간 분화 정도는 낮았다($G_{ST}$ = 0.064). 전체 11 집단에서 임의교배에 의한 편차는 0.311이였다. 전체 유전적 다양성는 $0{\sim}0.535$였다. 유전적 다양도 중 집단내 변이는 높았다($H_S$ = 0.305). 세대간 이주하는 개체수는 약 3.66으로 이 종의 한국내 집단간 유전자 흐름이 높음을 시사한다. 또한 라이트의 고정지수 분석 결과 많은 대립유전자좌위와 집단에서 이형접합자의 결핍이 존재하고 있었다. 집단간 유전적 동질성은 0.988이였다. 이는 맥문동의 분포지가 한국내 유사한 환경에 놓여 있고 집단이 방향적 동질성을 가지고 있음을 시사한다. Genetic diversity and population structure of eleven Liriope platyphylla (Liliaceae) populations in Korea were determined using genetic variation at 20 allozyme loci. The percent of polymorphic loci within the enzymes was 55.9%. Genetic diversity at the species level and at the population level was high(Hes = 0.178; Hep = 0.168, respectively), whereas the extent of the population divergence was relatively low ($G_{ST}$ = 0.064). $F_{IS}$, a measure of the deviation from random mating within the 11 populations, was 0.311. Total genetic diversity values ($H_T$) varied between 0.0 and 0.535, giving an average over all polymorphic loci of 0.323. The interlocus variation in within population genetic diversity ($H_S$) was high (0.305). An indirect estimate of the number of migrants per generation (Nm = 3.66) indicates that gene flow is high among Korean populations of the species. In addition, analysis of fixation indices revealed a substantial heterozygosity deficiency in some populations and at some loci. Mean genetic identity between populations was 0.988. It is highly probable that directional toward genetic uniformity in a relatively the homogenous habitat is thought to be operated among Korean populations of L. platyphylla.

과학전람회 고등학교 작품의 분석 및 교과서 내용과의 비교

허홍욱,허만규 韓國生物敎育學會 2003 생물교육 Vol.31 No.1

The study is to analyze the works at national science exhibitions for high school teachers and students for recent eleven years. The results were compared with the contents of high school science textbooks. The works by students were studied by 'observation' and 'investigation' activities as scientific methods, while teacher's works were researched by various scientific methods. The mean Shannon's diversity index (DJ) showed same trend for students and teachers. There was non-significant difference of the methods in student and teacher groups according to years, and the association between students and teachers were closely connected with methods. The correlation between the content of students' works and texts of high-school was highly positive for earth science and chemistry, whereas physics showed negative correlation. The correlation between students' works and the content of textbooks was positive in terms of the methods of study, but the correlation between teachers' works and the content of textbooks did not show similar pattern.