Chlorella vulgaris의 세포막에서 일어나는 Glycine 흡수과정에 관한 연구

權五溶,辛昌男,吳炅煥,李錦洙 충남대학교 자연과학연구소 1981 忠南科學硏究誌 Vol.8 No.1

In order to datemine that glycine uptake in the cell membrane of Chlorella vulgaris is depend on the specificity of substrate and energy required, it has been investigated on the reaction velocity of glycine and certain carrier. Some effects on the transmural fluxes of glycine across the cell membrane were also detemined. 1. The time course of glycine uptake was a linear function of time to early 10 min, and increased in the steady state slowly. 2. Glycine influx had shown apparent affinity in the range of 0.001 mM to 10 mM solution of glycine and followed Michaelis-Menten Kinetics(Km-0.16 mM, Vmax=0.31 nM2.0x10^7 cells min). 3. Glycine influx with 1 mA IAA was reduced about 50 percentage, suggesting that glycine uptake process is energy-dependent. 4. Glycine uptake was inhibited by glutamic acid, alanine, phenylalanine, proline, and methionine with the exception of histidine. It was suggested that the inhibition was competeted with some amino acids described above.

당근 種子 發芽 促進에 관한 硏究(4) : 당근 종자 발아에 있어서 몇가지 有機酸의 영향

권오용 安東敎育大學 1974 論文集 Vol.7 No.1

1. 0.001 M citric acid, 0.001 M tartaric acid. 0,001 M succinic acid, 0.001 M fumaric acid, 0.001 M malic acid 용액의 첨가처리에서 0,001 M citric acid, 0.001 M tartaric acid, 0.001 M succinic acid의 順으로 發芽率이 비교군보다 높았음을 알 수 있었다. 2. 이러한 發芽의 生理的 機作은 Kreb's cycle에 의하여 活動에너지의 流轉이 계속되고 있음을 보여 주는 것으로 0.001 M citric acid 첨가처리구에서 더 높은 發芽率이 나타났으며 또한 알맞는 미량의 유기산 첨가처리는 種子의 發芽率은 높이고 발아를 촉진시킬 수 있음을 나타내는 것이었다. The germination rate and growth rate of carrot seeds are obviously promoted by treatment with 0.001M tartaric acid and 0.001M succinic acid in the medium of various organic acids, and especially so by treatment with 0.001M citric acid. It is suggested by the author that the physiological mechanisms involved in this promotion are related to the functioning of the Kreb's citric acid cycle. For this reason, the germination and growth rate of carrot seeds treated with 0.001M citric acid are more accelerated than the other group and control group.

Pinus koraiensis와 Pinus parviflora의 樹高에 따른 氣孔數 變異에 關하여

權五容,辛昌男,吳炅煥,方在旭 충남대학교 자연과학연구소 1980 學術硏究誌 Vol.7 No.2

Stomata1 distribution in relation to the height of Pinus parviflora and Pinus koraiensis was studied 1. Stomata1 bands per needle of P parviflora and P koraiensis were 8.60±0.62 - 11.47±0.74, 14.07±1.03 - 16.60±0.99 and the number of stomata per band were 382.0±8.80 404.27±26.16, 1,326. 67±87.78 - 2,286.58±214.57, respectively 2. The number of stomatal band was significantly different between 3, 4m and 5, 6m in P parviflora and between 5, 6m and 7m in P. koraiensis Consequently, it is suggested that stomatal frequency gradient associated with increasing height above the ground is correlated with the greater degree of exposure to light.

밀(Triticum aestivum L.) 유식물이 질산 환원효소 활량에 대한 Sodium Azide의 영향

권오용,박흥순 충남대학교 자연과학연구소 1989 忠南科學硏究誌 Vol.16 No.1

These work was accomplished with 6 day-old wheat(Triticum aestivum L.)seedlings to determine the effect of the inhibitors by sodium azide on in vivo nitrate reductase activity. When the sodium azide was treated according to each concentration (0.01∼100mM), the inhibition occurred greatly in the shoots and it occurred slightly in the roots of wheat seedlings. After treating with 1.0mM sodium azide, optimum pH was 7.0 both in shoots and roots. Both the shoots and roots of wheat seedlings was greatly inhibited in the second hours, when 1.0mM sodium azide was treated as a function of time. The inhibition in 10mM KNO_3 concentration appeared greatly, when 1.0mM sodium azide was treated with each concentration of KNO_3.

호밀 (Secale cereale L.)유식물의 질산환원효소의 활량과 단백질함량에 관한 Cycloheximide의 영향

권오용,이영하,오희목,박흥순,이진철 충남대학교 자연과학연구소 1988 忠南科學硏究誌 Vol.15 No.2

This work was accomplished with 6 day-old rye (Secale cereale L.) seedlings to determine the effect of the antibiotics by cycloheximide on in vivo nitrate reductase activity, protein and RNA content. The nitrate reductase activity by treating 0.1μM and 0.1 mM cycloheximide was lower than that of the control group. The optimum pH for the nitrate reductase of the control group was 7.6 in the range of pH 5.6-8.0, but, in the case of 0.1mM cycloheximide treatment group, the optimum pH was decreased to 6.8 within the weak the acid region. The protein content of shoot and root in the 0.1μM and 0.1 mM cycloheximide treatment groups were respectively found at the 96%, 82% level of that in the control group. In the higher cycloheximide treatment (0.1 mM CH), not only nitrate reductase activity and protein content but also RNA content was greatly decreased. In this fact, it was suggested that the 0.1 mM cycloheximide had affected on the RNA polymerase. On the basis of there results, it was concluded that the decreased nitrate reductase activity by treating cycloheximide occurred primarily according to the decreased nitrate uptake, and the decreased protein and RNA content depended upon the results of the direct and indirect effect of cycloheximide on the protein synthesis.

몇가지 金屬이온이 밀 幼植物의 室酸還元 酵素 活量에 미치는 效果

權五溶,李天培,姜在善 충남대학교 자연과학연구소 1983 忠南科學硏究誌 Vol.10 No.2

The initial metabolism of nitrate to nitrite can be readily demonstrated in vivo by incubating plant tissue in the presence of nitrate in the dark under aerobic condition. All wheat seedlings during germination were in a dark incubator, and eight days-old seedlings were used for these experiments. Few other dater on nitrate reductase inhibition by metal ions can be found and no detailed studies of some inhibition types have been reported. For this reason, the authors have carried out some experiments on the inhibition types of nitrate reductase by some metal ions. 1mM CuCl and ZnCl_2 have inhibited nitrate reductase activity to a range of great degree (20-40%), while 1mM MnCl_2 and CoCl_2 have acted as less potent nitrate reductase inhibitors under 10% decreased condition. Relatively, 1mM KCl, MgCl_2 and CaCl_2 have shown the result acting as an activator of nitrate reductase. On the other hand, nitrate reductase levels have shown a phenomenon of fluctuation in response to changes depending upon 0.001-0.1mM concentration of some chloride salts, and shown constant value to a high concentration(1mM) of inhibited chloride salts. Consequently, it is assumed that the results of inhibition are belong to mixed type among various inhibitions, especially with respect to the nitrate reductase activity of wheat seedlings treated with CuCl, ZnCl_2, MnCl_2 and CoCl_2.

ESS Program의 인지적 영역에 대한 평가방법의 고찰(1) : 건전지와 꼬마전구의 단원을 중심으로 하여

권오용,손연규 安東敎育大學 1972 論文集 Vol.5 No.1

(1) ESS Program의 評價方法에 있어서 認知的 領域에 對한 評價基準을 세워 適用하고 實驗하였다. (2) 科學의 槪念은 아동스스로 科學的 方法에 依하여 效果的으로 發見할 수 있음을 알 수 있었다. (3) 敎授目標 卽 學習目標가 行動用語로 陳述되어 學習을 이끌어 나가므로써 認知的 領域에 대한 學習의 成就度가 높았다. (4) 이러한 이유에 依하여 새 自然科 學習 運營에서도 學習目標를 行動目標로 陳述하고 따라서 認知的 領域에 대한 行動用語를 中心으로 하는 評價가 效果的이라는 結論을 얻었다.

鷄龍山 陸松群落 및 리기다松群落의 林床植生에 關하여

權五溶,辛昌男,吳炅煥 충남대학교 자연과학연구소 1979 學術硏究誌 Vol.6 No.2

A study was carried out to ascertain the standing crops, species composition, and relationships between the standing crops and soil nutrients in the Pine forest and the Pitch pine forest of Gyeryong Mt. Species compositions of the Pine forest and the Pitch pine forest were 36 and 20 species, and dominant species of them was Festuca ovina . Total live standing crops of ground vegetation, 215.28-631.67g/㎡ and 188.62-446.13g/㎡, were highest in September, and net productivities were 13.02-169.95g/㎡ and 12.62-109.58g/㎡, respectively. Total live standing crops were significantly correlated with the water content and the organic matter of soil in each forest, and comparatively with the total nitrogen and the exchangeable phosphorus in the Pitch pine forest.

Bacillus cereus에 있어서 Phospholipase C활량에 관한 몇가지 계면활성제의 영향

권오용,윤희주,한수자 충남대학교 자연과학연구소 1987 忠南科學硏究誌 Vol.14 No.2

This study demonstrates the effects of four detergents and three on the activity change of phospholipase C which is prepared from the cultural broth of Bacillus cereus. This assay of phospholipase C activity was carried out with phosphatidylcholine micelles which were mixed with the detergents and sterols, with increase a molar ratio of these compounds to phosphatidylcholine. Phospholipase C was highly activated at 0.5 molar ratio of Tween 80-mixed micelles. In the case of Triton X-100, it was activated at higher molar ratio indicated 1.0. Sodium dodecyl sulfate was more effective than that of the other detergents at lower molar ratio. However sodium deoxycholate-mixed micelles increased the activity more 7 times than that of the control group. This maximum activation was occurred at 2.5 molar ratio and the turbidity was gradually decreased. Cholesterol and ergosterol increased the activity until molar ratio reached up to 0.9. But the activity was higher at 0.3 molar ratio for stigmasterol. Consequently, it was indicated that phospholipase C was activated with the supplement of detergents until the mixed micelles made a number of small and spherical shape.

Studies on the Acceleration of Germtnation in Carrot Seed (1) : Effect of some reagents, antibiotics, phytohormone and agricultural chemichals on the germination of carrot seed

권오용 安東敎育大學 1968 論文集 Vol.1 No.1

農作物中 가장 發芽가지연되고 있는 당근 種子예 대하여, 거기에 따르는 發芽 促進에 관한 硏究가 거의 없어서 이 硏究를 爲하여 數種의 試樂, 抗生物質, 植物性 홀몬, 種子消毒 處理에 의한 發芽에 미치는 影響을 우선 實驗 硏究하였다. 試藥處理區에서 有機溶劑인 Acetone, 抗生物質 處理區에서 Penicillin, 植物性 홀몬 處理區에서 Gibberellic acid, 種子消毒 處理區에서 Mercron 등의 處理에 依란 發芽가 促進됨을 알 수 있었다. 따라서 위의 實驗예서 植物性 홀몬과 有機溶劑가 種子發芽를 뚜렷하게 促進하여 줌음 알 수 있었다. 이것은 곧 당근 種子 속에 들어 있는 알지 못하는 抑制物質이 黃色의 sterol에 가까운 物質임을 推測케 할 수 있고 또한 植物性畏索로서는 Gibberellic acid에 依한 影響이 가장 큰 것임을 證明해 주는 것이다.