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葛根解酒湯 合 斷酒丸과 解酒枳葛湯이 實驗的 Alcoholism 생쥐의 行動에 미치는 影響
방혜연,黃義完 대한한방신경정신과학회 1994 동의신경정신과학회지 Vol.5 No.1
This experiment was designed to investigate the effects of Galgunhaeiutang + Danjuhwan and Haejujigaltang on the experimental behaviors of alcohol-inticxicated mice. ICR mice were used as subjects for alcohol intoxication in this experiment and sample group received Herb Medicine. The foot-step and rearing score were measured in mice. Also Rota-Rod treadmill & number of successful trials swimming time in the T-maze were done. And the following results were observed; 1. The food-step score was decreased in Galgunhaejutang + Danjuhwan group at the 8th hour, the difference was statistically significant. But Haejujigaltang group showed no significant difference. 2. The number of rearing was decreased in Galgunhaejutang + Danjuhwan group at the 3rd and 8th hour, the difference was statistical significant, while Haejujigaltang group showed no significant differnce. 3. The motor incoordination measered by the Rota-Rod treadmill was decreased in Galgunhaejutang + Danjuhwan group at the 8th hour, but Haejujigaltang group showed no significant difference. 4. The number of successful trials in T-maze showed no significant difference in both groups. 5. The passing time in T-maze was increased in Galgunhaejutang +Danjuhwan group at the 8th hour, but Haejujigaltang group showed no significant difference.
브로콜리에 있어서 突然變異劑 處理前과 處理後의 꽃봉오리 및 小花梗 培養
李洙聖,金辰熺,方惠蓮 中央大學校 食糧資源硏究所 1993 食糧資源硏究所 論文集 Vol.5 No.1
브로콜리의 꽃봉오리와 小花梗 培養法을 확립하였다. MS의 基本培地에 BA와 β-NAA를 각각 1㎎/l, 그리고 sucrose가 3% 첨가된 固體培地에 이들 조직을 치상하면 약 30일 후에 80%이상의 조직에서 그리고 組織當 평균 적어도 5개 이상의 순을 얻을 수 있다. 이 순은 植物生長調整劑가 첨가되지 않은 MS 基本培地에 sucrose가 2% 첨가된 固體培地로 옮겨주면 뿌리가 分化하여 완전한 식물체로 발달하였다. 한편 이들 조직을 突然變異劑 EMS(ethyle methane sulphonate)의 10mM또는 20M 용액에 각지 60분 또는 30분간 침지 처리한 후 배양함으로서 꽃봉오리에서는 무처리에 비해 약 50%의 순을 얻을 수 있다. 그리고 NMU(n-methyle-n-nitroso-urea)의 0.2mM용액에 20분정도 침지처리한 후 배양하면 꽃봉오리에서는 置床組織數의 약 60%에서, 그리고 小花梗에서는 약 30%에서 식물체를 얻을 수 있었다. In general, more than 80% of flower bud and pedicels of broccoli produced shoots when they were cultured on the solid medium of Murashinge and Skoog(MS) supplemented with 3% of sucrose and 1㎎/l of each of benzyl adenine(BA) and 2-naphthalene acetic acid(NAA). Shoots subcultured on the MS basic medium containing only 2% of sucrose developed enough roots to transplant for acclimatization. On the other hand, doses of chemical mutagenes, ethyl methane sulphonate(EMS) and n-methyl n-nitroso urea(MNU), were investigated for treatment to the flower bud and the pedicel. Flower buds treated with EMS either 10mM for 60 minutes or 20mM for 30 minutes produced shoots about 50% in the number compared to the non-treated one. In the case of MNU treatment, about 60% and 20% of the cultured buds and pedicels, respectively, produced shoots in the dose of 0.2mM for 20 minutes.