http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
생식선자극(生殖腺刺戟) 호르몬 처리가 산양의 배란난자(排卵卵子) 및 난포란(卵胞卵) 채취에 미치는 영향
송해범,입곡명 한국낙농학회 1986 韓國酪農學會誌 Vol.8 No.4
本 硏究는 成熱 및 未成熱 雌山羊에 生植腺剌載호르몬 처리가 排卵卵子 및 卵胞卵 채취에 미치는 영향을 검토하기 위하여 18 頭의 成熱雌山羊과 21 頭의 未成熱雌山羊을 公試하여 실시하였다. 實驗結果를 要約하면 다음과 같다. 1. 穎粒膜細胞에 둘러 쌓인 卵胞卵은 未成熱雌山羊에서 호르몬 무처리구 22.7개, PMSG처리구 54.2개, PMSG와 HCG 처리구 25.8개였으며, 成熱雌山羊에서는 PMSG 처리구 16.3개, PMSG와 HCG 처리구 7.8개였다. 2. 擴散된 穎粒膜細胞에 둘러 쌓인 卵胞卵은 PMSG와 HCG 처리구에서 成熱雌山羊은 1.7개, 未成熱雌山羊은 8.0개로 未成熱雌山羊이 成熱雌山羊보다 生植腺剌載호르몬 처리에 더욱 민감한 반응을 보였다. 3. PMSG와 HCG 처리구에서 排卵點은 成熱雌山羊은 7.4개, 未成熱雌山羊은 3.9개였으며, 採卵率은 각각 57%와 51%였다.
체외 배양시 과립막세포와 공배양된 돼지 난포란의 성숙과 수정
박수봉,박항균,입곡명 ( S . B . Park,H . K . Park,Akira Iritani ) 한국축산학회 1990 한국축산학회지 Vol.32 No.1
The present study was undertaken to examine the maturation and fertilization of porcine oocytes cocultured with granulosa cells. The results obtained were as follows. The percentages of oocytes reaching metaphase Ⅱ by GC -free culture and coculture with MGC and LGC were 32, 28 and 24% at 33h, respectively. In the culture from 33h to 42h, rates of oocyte maturation showed no difference among experimental groups. The fertilization rates of oocytes matured by GC-free culture and coculture with MGC and LGC were 92, 97 and 94%, respectively, Male pronucleus formation rates of oocytes matured by GC-free culture and coculture with MGC and LGC were 49, 55 and 78% respectively and markedly increased by coculture with LGC. The present results suggest that coculture with the membrana granulosa cells does not affect the incidence of maturation, and that pronucleus formation was promoted through coculture with LGC.
돼지 난포란의 성숙과 수정에 대한 성숙배지에 첨가된 난포액의 영향
박수봉,박항균,입곡명 ( S . B . Park,H . K . Park,Akira Iritani ) 한국축산학회 1990 한국축산학회지 Vol.32 No.2
The purpose of these experiments were to investigate the effect of follicular fluid supplemented to maturation medium on the maturation and fertilization of porcine follicular oocytes. The results obtained were as follows. In the media with MFF and LFF, the rates of maturation division of porcine oocytes in vitro were 82% and 80%, respectively, and the proportion of oocytes matured to metaphase Ⅱ become nearly constant after 39h of culture. However, the rates of maturation of porcine oocytes in medium with FCS 10% were 60% and 79% at 39 and 42h, respectively. The fertilization rate of oocyte cultured in the medium with FCS 10% is 99%. When the oocytes matured in medium with MFF and LFF were fertilized, fertilization rates were 42% and 54,%, respectively. The male pronucleus formation rates of oocytes cultured in the media with FCS 10% MFF 10% and LFF 10% concentrations were 54, 74 and 84%, respectively and were increased through culture in media supplemented with follicular fluid. The present results clearly demonstrate that follicular fluid promoted the maturation of oocytes and male pronucleus formation.
박수봉,박항균,입곡명 ( S . B . Park,H . K . Park,Akira Iritani ) 한국축산학회 1990 한국축산학회지 Vol.32 No.1
The present study was conducted to investigate the optimum conditions of in vitro fertilization by ejaculated boar spermatiozoa capacitated in a defined medium. When fresh ejaculated boar spermatozoa were preincubated for 4h with elevated sperm concentration fertilization rates were markedly increased. The fertilization rates by spermatozoa preincubated at the concentrations of 2, 4, 10, 20 and 40×10^8 cells/ml were 12, 30, 50, 76 and 82%, respectively, However, when spermatozoa preserved for 10-12h at 20℃ were preincubated for 4h with elevated sperm concentration fertilization rates were markedly decreased. The fertilization rates by spermatozoa preincubated at the concentrations of 2, 4, 10, 20 and 40×10^8 cells/ml at preincubation were 91, 77, 68, 44 and 8%, respectively. When oocytes matured in vitro were inseminated with spermatozoa at the concentration 1 × I0^6 cells/ml, rates of fertilization and polyspermy were 97 and 87%, respectively, after culture for 20h without changing medium. When oocytes were inseminated with spermatozoa at the different concentrations of 1, 0.5, 0.25 and 0.1×10^6 cells /ml and the medium was changed 5h after insemination, the fertilization rates were 75, 73, 61 and 30%, respectively. The rates of polyspermic fertilization were 58, 47, 40 and 47%, respectively, when oocytes were inseminated with different concentrations of spermatozoa, and there was no difference in the rates of polyspermy fertilization among groups with changing medium. The present results suggested that both sperm preincubation with high concentration and sperm preservation at 20℃ for 10-12h were effective for capacitation ejaculated boar spermatozoa and that a complete block of polyspermy was not achieved in this in vitro fertilization conditions.
송해범(H . B . Song),입곡명(A . Iritani) 한국축산학회 1988 한국축산학회지 Vol.30 No.11
This study was to develop a system by which goat follicular oocytes matured in culture can be fertilized in vitro with epididymal spermatozoa capacitated in a chemically defined medium, and to examine the effects of the following factors on fertilization in vitro; preservation before preincubation and concentration during preincubation of spermatozoa, presence of cumulus cells and culture periods of follicular oocytes. When freshly collected and preserved epididymal spermatozoa were preincubated at a concentration of 40-50 x 10^7 /ml, the fertilization rates were 51 and 57%, respectively. After further culture with spermatozoa for 18-24 h, 66, 89 and 26% of the oocytes with intact, with dispersed and without cumulus cells had matured to the 2nd metaphase. The highest fertilization rates (57%) were obtained when the oocytes with intact cumulus cells were cultured for 25 h and then inseminated with spermatozoa preincubated for 6 h in m-KRB solution. These results suggested that about half of the oocytes matured in culture could be normally fertilized with epididymal spermatozoa preincubated at a concentration of 40-50 x 10^7 /ml in m-KRB solution.
송해범(H . B . Song),입곡명(A . Iritani) 한국축산학회 1987 한국축산학회지 Vol.29 No.7
A total of 471 follicular oocytes obtained from the immature goats treated with PMSG alone, with PMSG and hCG or without gonadotropins was used in this experiments to study the maturation of follicular oocytes in vitro and the effects of the presence of cumulus cells on their maturation. There was no difference in the proportion of oocytes at the germinal vesicle stage between oocytes having intact cumulus cells and those without cumulus cells, when they were fixed and examined immediately after collection. The oocytes with dispersed cumulus cells obtained from goats treated with PMSG and hCG were almost at the 2nd metaphase stage at the time of collection, and they could be used for in vitro fertilization immediately after collection. After cultivation for 25-35 h, the overall maturation rate to the 2nd metaphase was 62% and 57% in the oocytes with intact cumulus cells from goat treated with PMSG alone and without gonadotropins, and it was 67% in those from goats treated with PMSG and hCG after cultivation for 25-30 h. The results of this experiment suggest that the follicular oocytes with intact cumulus cells could be normally matured in vitro, while the oocytes without cumulus cells could not.