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Plant regeneration via somatic embryogenesis from soybean cotyledon culture
Jong Lak Won(元鍾樂),Gyu Hwa Chung(鄭圭和) 한국육종학회 1988 한국육종학회지 Vol.20 No.3
The experiment was attempted to regenerate whole plant from somatic embryo using immature cotyledon cultures of five soybean varieties under different media. Normal shape somatic embryos were formed with 53.3% under MS medium supplemented with 1.0mg 2,4-D and 0.2mg/ℓ kinetin, and 32.2% on MS medium added with 8.0mg/ℓ NAA. Root and shoot was developed from the embryos under supplement with 0.1mg IBA and 0.25 mg ℓ│2-iP│of│ B5 medium, and whole plants were regenerated under further culture on hormoneless MS medium. Ulsan variety showed a good producer for somatic embryo among the lines applied. From subcultured somatic embryo callus, embryo formation is possible although their germination and organ differentiation is not easy.
韓昶烈,元鍾樂,崔光泰 韓國作物學會 1971 Korean journal of crop science Vol.- No.10
방사선조사시 열처리의 효과를 알기 위하여 수도종자에 방사선조사시 열전후처리를 행하였던바M1 세대의 생물학적인 반응 및 M2 세대의 변이율에 대해 몇가지 결과를 얻었다. 1. 방사선선량 20kR에서 열전처리는 방사선단독조사에 비해 생물학적인 장해를 적게하고 변이율을 증가시켰다. 2. 방사선단독조사 및 조사전열처리에 비해 heat shocking으로서 열후처리하는 것은 변이율을 증가시켰다. 3. 열전처리에 의해 M1 세대의 장해가 적고 변이율이증가되는 가장 효과적인 열처리온도 및 시간은 60℃ 에서 30분간이었다. 4. 열처리는 방사선단독조사에 비해 mutation spectrum을 달리했다. For the purpose of finding out the effect of heat treatment on biological response and mutation rate, rice seeds were heat treated before and after gamma irradiation. 1. At a dose of 20 KR, pre-irradiation heat treatment showed reduced biological damage and increased mutation rate as compared with non-heat treatment. 2. Mutation frequency was increased in post-treatment of heat shock than in pre-irradiation heat treatment and non-heat treatment. 3. Pre-irradiation heat treatment at 60~circC for 30 minutes markedly reduced the biological damage and increased the mutation rate. 4. Mutation spectrum in heat treatment was different from non-treatment.
Kim, Joon Chul,Won, Jong Lak,Seong, Min Woong 경상대학교 유전공학연구소 1984 遺傳工學硏究所報 Vol.3 No.-
Anatomical experiments on shoot formation from the zone near the tracheary elements of the habituated tobacco cells, its relation with peroxidase isozyme patterns and their activities were examined. Organ development was initiated with the change of a single vaculoated parenchyma cell from a mass of cells at 14 days after culture. The cells formed cell files, a region of meristemoid and clumps oriented from tracheery elements. The peroxidase activity was increased at the time of shoot initiation. The activity of shoot forming callus, especially in the region of meristemoid and shoot primordia was higher as compared to that of non-shoot forming callus, and the activity ratio of fast migrating isozyme group to the total activity was also high.
手稻高蛋白 突然變異系統과 母品種과의 交雜에 있어서 多發性遺傳因子의 發現에 關한 硏究
C. HARN(韓昶烈),J. L. WON(元鍾樂) 한국육종학회 1976 한국육종학회지 Vol.8 No.2
High protein rice mutant (M₁₂ plant generation in 1976) was obtained from the progeny of X-rayed Hokwang variety. Besides high protein, the mutant accompanied two other mutated characters of reduced culm length and earlier heading date. Crossing between the mutant and its mother variety was made (1) to examine the gene(s) controlling high protein, (2) to verify the pleiotropic (or linkage) relation among the three changed characters and (3) to test the possibility of intro-ducing the high protein gene(s) into mother variety. The assumption that a single pleiotropic recessive gene might be responsible for the three mutated characters was evidenced in F₂ by the segregation of mother and mutant types in the ratio of 3 : 1. Besides these parental types there were numbers of segregant groups in F₂. Among them were types which were mother-like phenoty-pically but had as high protein content as the mutant. These segregants were placed under further experiment for the confirmation of the character manifestation or fixation in later generations.
放射線處理한 벼 M₁이삭의 變異 sector에 關한 硏究
C. Harn(韓昶烈),J. L. Won(元鍾樂) 한국육종학회 1975 한국육종학회지 Vol.7 No.2
When mature seeds of cereal crops are treated with mutagenes, there occurs frequently the deficit of homo-recessive mutant in the M₁ generation, thus the segregation of recessiveness deviating from the expected ratio of 0.25. This has been considered caused partly by the function of the mutated gene and partly by the chimaeric nature of the M₁ panicle. Studies on the M₂ segregation inevitably led to the assumption of the size of mutated sector in the M₁ panicle and to the speculation on the number of initial cells (in the seed embryo) which would later form the sporogenous tissue of the M₁ panicle. So far the views concerning the initial cell have been conflicting and controvertial. X-rayed rice seeds were sown directly in the pots and all the tillers including the lower ones were induced to full growth to examine whether there exists any difference in the segregation ratio among the tillers. No definite tendencies or regularities in the segregation ratio among the tillers were observed. If the number of initial cells varies with the order of tiller emergence, i.e., main culm having several and the upper tillers having only one cell, there would be certain definite tendency in the segregation ratio among the tillers, with the upper tillers approaching the expected ratio, which was not proved in the present experiment. As it is assumed that the mutated cell, mutated gamete, and homo-recessive mutant might be affected by many factors, it does’nt seem to be correct to speculate the sector size in the M₁ panicle and the number of initial cells in the seed embryo simply by the M₂ sgregation ratio.