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Plant regeneration via somatic embryogenesis from soybean cotyledon culture
Jong Lak Won(元鍾樂),Gyu Hwa Chung(鄭圭和) 한국육종학회 1988 한국육종학회지 Vol.20 No.3
The experiment was attempted to regenerate whole plant from somatic embryo using immature cotyledon cultures of five soybean varieties under different media. Normal shape somatic embryos were formed with 53.3% under MS medium supplemented with 1.0mg 2,4-D and 0.2mg/ℓ kinetin, and 32.2% on MS medium added with 8.0mg/ℓ NAA. Root and shoot was developed from the embryos under supplement with 0.1mg IBA and 0.25 mg ℓ│2-iP│of│ B5 medium, and whole plants were regenerated under further culture on hormoneless MS medium. Ulsan variety showed a good producer for somatic embryo among the lines applied. From subcultured somatic embryo callus, embryo formation is possible although their germination and organ differentiation is not easy.
Organ differentiation from callus of immature cotyledon in soybean, glycine max(L.) merr
Kwon Yawl Chang(張權烈),Jong Lak Won(元鍾樂),Gyu Hwa Chung(鄭圭和) 한국육종학회 1987 한국육종학회지 Vol.19 No.2
The experiments were conducted to investigate organ differentiation from callus-derived embryoid under various media using immature cotyledon of soybean line Baemkong. 1.0mg 2,4-D plus 2.0 mg kinetin or 0.2mg BAP per litre on MS basal medium were effective growth regulators for callus induction. Meristematic structure was observed from the callus induced by 1.0mg 2,4-D plus 0.2mg kinetin per litre on MS basal medium in which callus growth showed best responses. Embryoid from yellowish green callus and leaflet from the embryoid were differentiated on the nutritional condition subcultured with 0.005 mg pieloram plus 2.0mg BAP per litre on MS basal medium after induction of callus oil the medium with 1.0 mg 2,4-D plus 2.0 mg kinetin per litre on MS in immature cotyledon.
Kim, Joon Chul,Won, Jong Lak,Seong, Min Woong 경상대학교 유전공학연구소 1984 遺傳工學硏究所報 Vol.3 No.-
Anatomical experiments on shoot formation from the zone near the tracheary elements of the habituated tobacco cells, its relation with peroxidase isozyme patterns and their activities were examined. Organ development was initiated with the change of a single vaculoated parenchyma cell from a mass of cells at 14 days after culture. The cells formed cell files, a region of meristemoid and clumps oriented from tracheery elements. The peroxidase activity was increased at the time of shoot initiation. The activity of shoot forming callus, especially in the region of meristemoid and shoot primordia was higher as compared to that of non-shoot forming callus, and the activity ratio of fast migrating isozyme group to the total activity was also high.