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RISS 인기검색어
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- 저자 : (Norlin J . Benevenga) (검색결과 2 건)
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쥐에 있어서 카제인 , 건조우유 , 건조요구르트 또는 유산균 함유 사료 급여가 장내 유당의 소화와 발효에 미치는 영향
김규일(Kyu Il Kim),(Norlin J . Benevenga),(Robert L . Bradley) 한국축산학회 1988 한국축산학회지 Vol.30 No.12
Growing adult male rats (6 /diet) were fed the following diets for 3 weeks; casein (20 % casein, control), milk (55 % dried milk) and yogurt (55 % dried yogurt), each with or without a supplement of 2 % of a Lactobacillus acidophilus culture. The diets containing milk and yogurt had 25 % lactose. The fraction of lactose which was not digested in the small intestine was determined by using a lactose /marker ratio in the terminal ileum. The rate of lactose fermentation and the rate of accumulation of fermentation products in the contents of cecum -[ -colon were measured by incubating the contents at 37℃ in 0.1 M phosphate buffer (pH 6.5) containing 25 mM lactose and 2 uCi of [U-^14C]lactose. The fraction of lactose which was not digested in the small intestine was not (P$gt;0.05) different among the dietary treatments (0.24-0,32). Lactase activity in the cecum+colon contents of rats fed diets containing milk or yogurt was more than twice that of rats fed the casein diet. The amount of galactose fermented during the 30-minute incubation in the contents of rats fed diets containing milk or yogurt was 3 times that found in rats fed the casein diet. Radioactivity recovered in 15,00O× g supernatants of the contents incubated for 30 minutes and inactivated with sulfuric acid was lower(P$lt;0.01) in rats fed diets containing milk or yogurt than in rats fed the casein diet, while that recovered in the pellet or CO₂ was higher (P$lt;0.01). The rate of lactic acid and short-chain fatty acid production from lactose in the large intestinal contents of rats fed diets containing milk or yogurt was approximately 3 times higher than that in rats fed the casein diet, reflecting the lactase activity and the galactose fermentation rate. No significant effects of the lactobacillus culture on the lactose digestion and fermentation were noted. These results indicate that dietary milk or yogurt (or lactose) increases the fermentation of lactose and galactose in the large intestine, but there is no difference between dried yogurt and dried milk in increasing lactose fermentation in the large intestine.
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Kim, KYU-lI,Lee, Wang-SIK,Benevenga, Norlin J. 濟州大學校 農科大學 動物科學硏究所 1998 動物科學論叢 Vol.13 No.1
Three studies were done to determine the effect of feeding diets containing high levels of a readily fermentable carbohydrate (lactose in milk or yogurt, or pure lactose) or an undigestible. unfermentable diluent (α-cellulose) on urease (EC 3.5.1 5) activity and net mmonia production in the rat gastrointestinal (GI) contents. Rats (170-200 g) were fed a control diet or diets containing 55% dried milk or 55% dried yogurt. 25% lactose or 10% a-cellulose. Feeding diets containing milk or yogurt decreased urease activrty to -1 1 % of the control value in the small intestine (on the basis of grams of collected contents or total contents), and to 50% in the large intestine (only on the basis of grams of collected contents). Feeding the diet containing 25% lactose also decreased urease activity (on the basis of grams of collected contents or total contents) to about 20% of the control value in the small intestine, but not (P > 0.05) in the large intestine. Net ammonia production rate was correlated (r^(2) = 0.98) with urease activity in the large intestinal contents, and the rate of ammonia production from ureolysis represented about two thirds of the total. Feeding the cellulose diet decreased (P < 0.05) both urease activity and net ammonia production in the large intestine to -30% of the control value. Weights of tissue and contents of the large intestine were much higher (P < 0.01) in rats fed diets containing milk products or lactose than in the control rats, but were not affected by consumption of the cellulose diet. Results of our studies indicate that feeding diets containing high levels of milk products (lactose) or cellulose reduces urease activity and net ammonia production in the rat intestine, and thus may be beneficial for improving animal and human health. J. Nutr. 128: 1186-1191, 1998.
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