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A Fermentation Strategy for Anti-MUC1 C595 Diabody Expression in Recombinant Escherichia coli
John Chi-Wei Lan,Tau Chuan Ling,Grant Hamilton,Andrew Lyddiatt 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.5
The development of fermentation conditions for the production of C595 diabody fragment (dbFv) in E. coli HB2151 clone has been explored. Investigations were carried out to study the effect of carbon supplements over the expression period, the comparison of C595 dbfv production in synthetic and complex media, the influence of acetic acid upon antibody production, and comparison of one-stage and two-stage processes operated at batch or fed-batch modes in bioreactor. Yeast extract supplied during expression yielded more antibody fragment than any other carbon supply. The synthetic medium presented higher specific productivity (0.066 mg dbFv g-1 dry cell weight) when compared to the complex medium (0.044 mg dbFv g-1 DCW). The comparison of fermentation strategies demonstrated that (1) one-stage fed-batch fermentation performed higher C595 dbFv production than that operated in batch mode which was significantly affected by acetate concentration; (2) a two-stage batch operation could enhance C595 dbFv production. It was found that a concentration of 12.3 mg L-1 broth of C595 dbFv and a cell concentration of 10.8 g L-1 broth were achieved at the end of two-stage operation in 5-L fermentation.
Conversion of waste textile cellulose fibers into heavy metal adsorbents
John Kwame Bediako,Wei Wei,윤영상 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.43 No.-
Fibrous adsorbents were prepared by carboxymethylation of waste textilefibers and were used for Cd(II)sorption in comparison with Dowex Mac-3 (DM-3). The prepared sorbents were designatedcarboxymethyl cellulose-Lyocell strands (CMC-LS) and carboxymethyl cellulose-Lyocell fabrics (CMCLF). Kinetic and isotherm studies indicated that CMC-LS had the fastest sorption rate with high affinityconstants and good column application potential. For the same packed column volume, CMC-LS reached70% of its capacity whilst DM-3 reached57%. 100% desorption was achieved using 0.1 M EDTAor HCl. This study therefore suggests a way to recycle wastefibers into adsorbents for treating heavymetal-polluted waters.
Removal of Cd(II) by poly(styrenesulfonic acid)-impregnated alginate capsule
Wei, Wei,Bediako, John Kwame,Kim, Sok,Yun, Yeoung-Sang ELSEVIER SCIENCE B.V.; AMSTERDAM 2016 JOURNAL- TAIWAN INSTITUTE OF CHEMICAL ENGINEERS Vol.61 No.-
<P><B>Abstract</B></P> <P>In this study, a capsule type sorbent of poly(styrenesulfonic acid)-impregnated alginate capsule (PSSA-AC) was developed by using a simple and moderate method. PSSA was used as a model encapsulated material because of its large number of sulfonic acid groups which have high affinity toward Cd(II). PSSA-AC was prepared by dropping solution A (mixture of 10% (w/v) PSSA + 1% CaCl<SUB>2</SUB>) into B solution (0.6% (w/v) sodium alginate). Microscope images revealed that PSSA was well encapsulated inside of the alginate shell. The pH edge experiment indicated that Cd(II) sorption was influenced marginally by the experimental pH range. The maximum Cd(II) uptake by PSSA-AC was as high as 184.12 ± 6.30 mg/g. The kinetic and FTIR studies demonstrated that ion-exchange was the main sorption mechanism. The PSSA-AC could be regenerated by using 2% CaCl<SUB>2</SUB> as a desorbing agent.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PSSA-AC was successfully developed using a simple and moderate method. </LI> <LI> The maximum Cd(II) uptake was 184.12 ± 6.30 mg/g using Langmuir isotherm model. </LI> <LI> The Cd(II)-loaded PSSA-AC was easily regenerated by using 2% CaCl<SUB>2</SUB> solution. </LI> <LI> The overall sorption/desorption mechanism was cation ion-exchange between Cd(II) and Ca(II). </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Chien Wei Ooi,Beng Ti Tey,Siew Ling Hii,Arbakariya Ariff,Ho Shing Wu,John Chi Wei Lan,Ruey Shin Juang,Siti Mazlina Mustapa Kamal,Tau Chuan Ling 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.6
An aqueous two-phase purification process was employed for the recovery of Burkholderia pseudomallei from fermentation broth. The partition behavior of B.pseudmallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts. Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study An aqueous two-phase purification process was employed for the recovery of Burkholderia pseudomallei from fermentation broth. The partition behavior of B.pseudmallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts. Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study
A Fermentation Strategy for Anti-MUC1 C595 Diabody Expression in Recombinant Escherichia Coli
Lan, John Chi-Wei,Ling, Tau Chuan,Hamilton, Grant,Lyddiatt, Andrew The Korean Society for Biotechnology and Bioengine 2006 Biotechnology and Bioprocess Engineering Vol.11 No.5
The development of fermentation conditions for the production of C595 diabody fragment (dbFv) in E. coli HB2151 clone has been explored. Investigations were carried out to study the effect of carbon supplements over the expression period, the comparison of C595 dbfv production in synthetic and complex media, the influence of acetic acid upon antibody production, and comparison of one-stage and two-stage processes operated at batch or fed-batch modes in bioreactor. Yeast extract supplied during expression yielded more antibody fragment than any other carbon supply. The synthetic medium presented higher specific productivity (0.066 mg dbFv $g^{-1}$ dry cell weight) when compared to the complex medium (0.044 mg dbFv $g^{-1}$ DCW). The comparison of fermentation strategies demonstrated that (1) one-stage fed-batch fermentation performed higher C595 dbFv production than that operated in batch mode which was significantly affected by acetate concentration; (2) a two-stage batch operation could enhance C595 dbFv production. It was found that a concentration of 12.3 mg $L^{-1}$ broth of C595 dbFv and a cell concentration of 10.8g $L^{-1}$ broth were achieved at the end of two-stage operation in 5-L fermentation.
Bediako, John Kwame,Reddy, D. Harikishore Kumar,Song, Myung-Hee,Wei, Wei,Lin, Shuo,Yun, Yeoung-Sang Elsevier 2017 Journal of environmental chemical engineering Vol.5 No.1
<P><B>Abstract</B></P> <P>A facile modification of waste textile cellulose Lyocell fibers with tripolyphosphate and application of the adsorbent thereof to the treatment of Pb(II)-contaminated wastewater was investigated. Characterization and batch adsorption studies were conducted to understand the characteristics of the prepared adsorbent and its metal binding mechanisms. The adsorbent showed 100% adsorptive removal efficiency of Pb(II) at initial concentrations up to 300mg/L, and the adsorption performance was significant even at low pH ranges. The adsorption isotherm followed the Langmuir model, and the kinetic was described by the pseudo-first and pseudo-second order models. By comparison, the adsorbent showed strong competitiveness to existing ones especially in terms of the adsorption capacity, pH, and kinetics of adsorption, and could be reused. Considering its cheap source and simplicity of preparation, the adsorbent could be applied as a low-cost material for heavy metal scavenging from wastewater streams.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A facile method for preparing cellulosic heavy metal adsorbent with phosphate chelating groups was presented. </LI> <LI> High selectivity of Pb(II) was found in multi-metal systems. </LI> <LI> Hundred percent adsorption efficiency of Pb(II) at initial concentrations up to 300mg/L was achieved. </LI> <LI> Adsorption capacity was very high even at low pH. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Tamgue, Ousman,Chai, Cheng-Sen,Hao, Lin,Zambe, John-Clotaire Daguia,Huang, Wei-Wei,Zhang, Bin,Lei, Ming,Wei, Yan-Ming Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10
The histone methyltransferase EZH2 (enhancer of zeste homolog 2) plays critical roles in prostate cancer (PCa) development and is a potential target for PCa treatment. Triptolide possesses anti-tumor activity, but it is unknown whether its therapeutic effect relates with EZH2 in PCa. Here we described EZH2 as a target for Triptolide in PCa cells. Our data showed that Triptolide suppressed PCa cell growth and reduced the expression of EZH2. Overexpression of EZH2 attenuated the Triptolide induced cell growth inhibition. Moreover, Triptolide treatment of PC-3 cells resulted in elevated mRNA levels of target genes (ADRB2, CDH1, CDKN2A and DAB2IP) negatively regulated by EZH2 as well as reduced mRNA levelsan of EZH2 positively regulated gene (cyclin D1). Our findings suggest the PCa cell growth inhibition mediated by Triptolide might be associated with downregulation of EZH2 expression and the subsequent modulation of target genes.