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      • KCI등재

        Genetic diversity analysis based on sequence-related amplified polymorphism (SRAP) reveals the genetic background of Xinan tree peony group cultivars from different geographical provenances

        Zongyan Li,Kunmei Yang,Yanrong Zhao,Ping Lin 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.4

        The Xinan tree peony cultivar group, one of four Chinese peony cultivar groups, is distributed sparsely throughout Southwest China. This study aimed to characterize its genetic diversity and its genetic relationships with other peony groups. A total of 96 specimens from seven groups of different geographical provenances were collected and their genetic similarity was analyzed based on sequence-related amplified polymorphism molecular markers. Twenty primer pairs were selected to detect DNA polymorphisms. A total of 868 bands were generated with an average of 43.4 bands per primer pair and 98.39% polymorphic ratio. The genetic similarity coefficient varied from 0.5081 to 0.9173. The closest phylogenetic relationship was found between hybrids 2 and 3 of Paeonia rockii, while the farthest genetic relationship was between P. lutea and P. suffruticosa ‘Bangningzi’. Among the 20 Xinan samples the genetic similarity coefficient ranged from 0.6351 to 0.8710, revealing slight genetic variation. The 96 cultivars clustered into five branches at the genetic similarity coefficient of 0.6419. The results revealed that the Xinan group has a closer relationship with Japanese, French and American groups. Additionally, these results show that the phylogenetic relationships of different tree peony germplasms correspond to their origins and are primarily related with flower color.

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        Generalized Joint Channel-Network Coding in Asymmetric Two-Way Relay Channels

        Shen, Shengqiang,Li, Shiyin,Li, Zongyan Korean Society for Internet Information 2016 KSII Transactions on Internet and Information Syst Vol.10 No.12

        Combining channel coding and network coding in a physical layer in a fading channel, generalized joint channel-network coding (G-JCNC) is proved to highly perform in a two-way relay channel (TWRC). However, most relevant discussions are restricted to symmetric networks. This paper investigates the G-JCNC protocols in an asymmetric TWRC (A-TWRC). A newly designed encoder used by source nodes that is dedicated to correlate codewords with different orders is presented. Moreover, the capability of a simple common non-binary decoder at a relay node is verified. The effects of a power match under various numbers of iteration and code lengths are also analyzed. The simulation results give the optimum power match ratio and demonstrate that the designed scheme based on G-JCNC in an A-TWRC has excellent bit error rate performance under an appropriate power match ratio.

      • KCI등재

        Generalized Joint Channel-Network Coding in Asymmetric Two-Way Relay Channels

        ( Shengqiang Shen ),( Shiyin Li ),( Zongyan Li ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.12

        Combining channel coding and network coding in a physical layer in a fading channel, generalized joint channel-network coding (G-JCNC) is proved to highly perform in a two-way relay channel (TWRC). However, most relevant discussions are restricted to symmetric networks. This paper investigates the G-JCNC protocols in an asymmetric TWRC (A-TWRC). A newly designed encoder used by source nodes that is dedicated to correlate codewords with different orders is presented. Moreover, the capability of a simple common non-binary decoder at a relay node is verified. The effects of a power match under various numbers of iteration and code lengths are also analyzed. The simulation results give the optimum power match ratio and demonstrate that the designed scheme based on G-JCNC in an A-TWRC has excellent bit error rate performance under an appropriate power match ratio.

      • KCI등재

        Codon optimization of the rabbit hemorrhagic disease virus (RHDV)capsid gene leads to increased gene expression in Spodoptera frugiperda 9 (Sf9) cells

        Jingpeng Gao,Chunchun Meng,Zongyan Chen,Chuanfeng Li,Guangqing Liu 대한수의학회 2013 Journal of Veterinary Science Vol.14 No.4

        Rabbit hemorrhagic disease (RHD) is contagious and highly lethal. Commercial vaccines against RHD are produced from the livers of experimentally infected rabbits. Although several groups have reported that recombinant subunit vaccines against rabbit hemorrhagic disease virus (RHDV) are promising, application of the vaccines has been restricted due to high production costs or low yield. In the present study, we performed codon optimization of the capsid gene to increase the number of preference codons and eliminate rare codons in Spodoptera frugiperda 9 (Sf9) cells. The capsid gene was then subcloned into the pFastBac plasmid, and the recombinant baculoviruses were identified with a plaque assay. As expected,expression of the optimized capsid protein was markedly increased in the Sf9 cells, and the recombinant capsid proteins self-assembled into virus-like particles (VLPs) that were released into the cell supernatant. Rabbits inoculated with the supernatant and the purified VLPs were protected against RHDV challenge. A rapid, specific antibody response against RHDV was detected by an ELISA in all of the experimental groups. In conclusion, this strategy of producing a recombinant subunit vaccine antigen can be used to develop a low-cost, insect cell-derived recombinant subunit vaccine against RHDV.

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