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Joint Deployment and Trajectory Optimization in UAV-Assisted Vehicular Edge Computing Networks
Zhiwei Wu,Zilin Yang,Chao Yang,Jixu Lin,Yi Liu,Xin Chen 한국통신학회 2022 Journal of communications and networks Vol.24 No.1
As the general mobile edge computing (MEC)scheme cannot adequately handle the emergency communicationrequirements in vehicular networks, unmanned aerial vehicle(UAV)-assisted vehicular edge computing networks (VECNs) areenvisioned as the reliable and cost-efficient paradigm for themobility and flexibility of UAVs. UAVs can perform as thetemporary base stations to provide edge services for road vehicleswith heavy traffic. However, it takes a long time and huge energyconsumption for the UAV to fly from the stay charging stationto the mission areas disorderly. In this paper, we design a predispatchUAV-assisted VECNs system to cope with the demandof vehicles in multiple traffic jams. We propose an optimalUAV flight trajectory algorithm based on the traffic situationawareness. The cloud computing center (CCC) server predictsthe real-time traffic conditions, and assigns UAVs to differentmission areas periodically. Then, a flight trajectory optimizationproblem is formulated to minimize the cost of UAVs, while boththe UAV flying and turning energy costs are mainly considered. Inaddition, we propose a deep reinforcement learning(DRL)-basedenergy efficiency autonomous deployment strategy, to obtain theoptimal hovering position of UAV at each assigned mission area. Simulation results demonstrate that our proposed method canobtain an optimal flight path and deployment of UAV with lowerenergy consumption.
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Zhuhua Chan,Tianhua Zhong,Zhiwei Yi,Jing Xiao,Runying Zeng 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.5
The aim of this work is to enhance enduracidin production by Streptomyces sp. MC079. Based on the time course analysis of the specific cell growth rate and specific enduracidin formation rate, a two–stage pH control strategy was proposed to improve enduracidin production by shifting the culture pH from 5.5 to 5.8 after 112 h of cultivation. By applying this pH control strategy, enduracidin concentration and productivity was 51.2 and 65.0% higher than results with uncontrolled pH batch fermentation. For further enhancement of enduracidin production, the effects of constant–rate feeding and pH-shift feeding strategy were investigated. The results indicated that the pH-shift feeding strategy increased the maximum concentration and productivity of enduracidin to 61.37 mg/L and 0.697 mg/L/h in the constant–rate feeding fermentation process. This is 73.3 and 88.9% higher than results with uncontrolled pH batch fermentation, respectively. The obtained optimal pH shift feeding strategy may be useful for the industrial–scale microbial production of enduracidin.
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( Chao Gao ),( Min Jin ),( Zhiwei Yi ),( Runying Zeng ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.11
A novel sulfatase gene, ary423 (1,536 bp ORF), encoding a protein of 511 amino acids with a calculated molecular mass of 56 kDa, was identified from Flammeovirga pacifica, which was isolated from deep-sea sediments of west Pacific Ocean. Amino acid sequence analysis revealed that Ary423 possessed a conserved C-X-A-X-R motif, which was recognized as the sulfatase signature. Phylogenetic analysis suggested that Ary423 belonged to arylsulfatases. After heterologous expression in Escherichia coli cells, the recombinant Ary423 was purified with a N i+ affinity column, and was shown to be highly active at a broad range of temperatures from 30° to 70°C, with maximum activity at 40°C. Furthermore, recombinant Ary423 retained more than 70% and 40% of its maximum activity after 12 h of incubation at 50°C and 60°C, respectively, exhibiting good thermostability at high temperatures. The optimal pH for Ary423 was determined to be 8.0 and the activity of Ary423 could be slightly enhanced by Mg2+. The recombinant enzyme could hydrolyze sulfate ester bonds in pnitrophenyl sulfate (NPS) and Asparagus crude polysaccharides with a specific activity of 64.8 U/mg and 25.4 U/mg, respectively. These favorable properties could make Ary423 attractive for application in the desulfating process of agar production.
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Zhang, Jinglan,Shi, Xiaomin,Li, Yehua,Kim, Beom-Jun,Jia, Junling,Huang, Zhiwei,Yang, Tao,Fu, Xiaoyong,Jung, Sung Yun,Wang, Yi,Zhang, Pumin,Kim, Seong-Tae,Pan, Xuewen,Qin, Jun Elsevier 2008 Molecular cell Vol.31 No.1
<P><B>Summary</B></P><P>Sister chromatid cohesion is normally established in S phase in a process that depends on the cohesion establishment factor Eco1, a conserved acetyltransferase. However, due to the lack of known in vivo substrates, how Eco1 regulates cohesion is not understood. Here we report that yeast Eco1 and its human ortholog, ESCO1, both acetylate Smc3, a component of the cohesin complex that physically holds the sister chromatid together, at two conserved lysine residues. Mutating these lysine residues to a nonacetylatable form leads to increased loss of sister chromatid cohesion and genome instability in both yeast and human. In addition, we clarified that the acetyltransferase activity of Eco1 is essential for its function. Our study thus identified a molecular target for the acetyltransferase Eco1 and revealed that Smc3 acetylation is a conserved mechanism in regulating sister chromatid cohesion.</P>
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