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Heejeong Kim,Daekil Song,Yeonji Kook,Yihyung Chung 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
This study was carried out to identify and compare the antioxidant capacity of three different extraction methods of Cranberry extract (i.e. HWE; hot water extraction; AUE, ultrasonic extraction; EE, 70% ethanol extraction;) and the effect of the human kidney epithelial cell(293T cell) proliferation was examined. Analyses of phenolic compounds were carried out by measuring the total polyphenol, total anthocyanin and proanthocyanidin content. Cell proliferation was determined using WST assay. The results showed a significant increase in the level of phenolic compounds in the Cranberry extract using 70% ethanol(EEE) compared to those using hot water extraction(HWE) or ultrasonic extraction(AUE). The 70% ethanol extraction(EEE) showed proliferation of human kidney epithelial cells compared to those using hot water extraction(HWE) or ultrasonic extraction(AUE). In conclusion, compared to other two extracts, usage of 70% ethanol could improve the nutritional value of Cranberry by enhancing the phenolic compounds and human kidney epithelial cell proliferation. These results suggest that Cranberry extract would have Anti-oxidant effects which needs to be further studied in rodents.
( Juyeon Lee ),( Minkyoung Kang ),( Jiseon Yoo ),( Sujeong Lee ),( Minji Kang ),( Bohyun Yun ),( Jong Nam Kim ),( Hyoungsun Moon ),( Yihyung Chung ),( Sangnam Oh ) 한국미생물생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.7
Sarcopenia is defined as loss of muscle mass and strength due to aging. Recent studies show that sarcopenia may improve via the gut-muscle axis, suggesting that gut health may affect muscle phenotypes. In this study, we aimed to investigate the ability of Lactobacillus rhamnosus JY02 as a probiotic strain isolated from kimchi to alleviate sarcopenia. L. rhamnosus JY02-conditioned medium (CM) reduced dexamethasone (DEX)-induced myotube diameter atrophy and expression of muscle degradation markers (MuRF1 and atrogin-1) in C2C12 cells. The amelioration of sarcopenia was investigated by measuring body composition (lean mass), hand grip strength, myofibril size (using histological analysis), and mRNA and protein expression of muscle-related factors in a DEX-induced mouse model. The results of these analyses showed that L. rhamnosus JY02 supplementation promoted the production of muscle-enhancement markers (MHC Iβ, MHC IIα, and Myo-D) and reduced both the production of muscle degradation markers and the symptoms of muscle atrophy (loss of lean mass and muscle strength). We also found decreased levels of pro-inflammatory cytokines (IL-6, IFN- γ) and increased levels of anti-inflammatory cytokines (IL-10) in the serum of DEX+JY02-administered mice compared to those in DEX-treated mice. Overall, these results suggest that L. rhamnosus JY02 is a potent probiotic supplement that prevents sarcopenia by suppressing muscle atrophy.