Arctigenin Increases Hemeoxygenase-1 Gene Expression by Modulating PI3K/AKT Signaling Pathway in Rat Primary Astrocytes

( Yeon Hui Jeong ),( Jin Sun Park ),( Dong Hyun Kim ),( Hee Sun Kim ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.6

In the present study, we found that the natural compound arctigenin inhibited hydrogen peroxide-induced reactive oxygen species (ROS) production in rat primary astrocytes. Since hemeoxygenase-1 (HO-1) plays a critical role as an antioxidant defense factor in the brain, we examined the effect of arctigenin on HO-1 expression in rat primary astrocytes. We found that arctigenin increased HO-1 mRNA and protein levels. Arctigenin also increases the nuclear translocation and DNA binding of Nrf2/c-Jun to the antioxidant response element (ARE) on HO-1 promoter. In addition, arctigenin increased ARE-mediated transcriptional activities in rat primary astrocytes. Further mechanistic studies revealed that arctigenin increased the phosphorylation of AKT, a downstream substrate of phosphatidylinositol 3-kinase (PI3K). Treatment of cells with a PI3K-specific inhibitor, LY294002, suppressed the HO-1 expression, Nrf2 DNA binding and ARE-mediated transcriptional activities in arctigenin-treated astrocyte cells. The results collectively suggest that PI3K/AKT signaling pathway is at least partly involved in HO-1 expression by arctigenin via modulation of Nrf2/ARE axis in rat primary astrocytes.

Kalopanaxsaponin A Exerts Anti-Inflammatory Effects in Lipopolysaccharide-Stimulated Microglia via Inhibition of JNK and NF-κB/AP-1 Pathways

( Yeon Hui Jeong ),( Jin Won Hyun ),( Tien Kim Van Le ),( Dong Hyun Kim ),( Hee Sun Kim ) 한국응용약물학회 2013 Biomolecules & Therapeutics(구 응용약물학회지) Vol.21 No.5

Microglial activation plays an important role in the development and progression of various neurological disorders such as cerebral ischemia, multiple sclerosis, and Alzheimer`s disease. Thus, controlling microglial activation can serve as a promising therapeutic strategy for such brain diseases. In the present study, we showed that kalopanaxsaponin A, a triterpenoid saponin isolated from Kalopanax pictus, inhibited inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and tumor necrosis factor (TNF)-α expression in lipopolysaccharide (LPS)-stimulated microglia, while kalopanaxsaponin A increased anti-infl ammatory cytokine interleukin (IL)-10 expression. Subsequent mechanistic studies revealed that kalopanaxsaponin A inhibited LPS-induced DNA binding activities of NF-κB and AP-1, and the phosphorylation of JNK without affecting other MAP kinases. Furthermore, kalopanaxsaponin A inhibited the intracellular ROS production with upregulation of anti-infl ammatory hemeoxygenase-1 (HO-1) expression. Based on the previous reports that JNK pathway is largely involved in iNOS and proinfl ammatory cytokine gene expression via modulating NF-κB/AP-1 and ROS, our data collectively suggest that inhibition of JNK pathway plays a key role in anti-infl ammatory effects of kalopanaxsaponin A in LPS-stimulated microglia.

Lonchocarpine Increases Nrf2/ARE-Mediated Antioxidant Enzyme Expression by Modulating AMPK and MAPK Signaling in Brain Astrocytes

( Yeon-hui Jeong ),( Jin-sun Park ),( Dong-hyun Kim ),( Hee-sun Kim ) 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.6

Lonchocarpine is a phenylpropanoid compound isolated from Abrus precatorius that has anti-bacterial, anti-inflammatory, antiproliferative, and antiepileptic activities. In the present study, we investigated the antioxidant effects of lonchocarpine in brain glial cells and analyzed its molecular mechanisms. We found that lonchocarpine suppressed reactive oxygen species (ROS) production and cell death in hydrogen peroxide-treated primary astrocytes. In addition, lonchocarpine increased the expression of antioxidant enzymes, such as heme oxygenase-1 (HO-1), NAD(P)H:quinone oxidoreductase 1 (NQO1), and manganese superoxide dismutase (MnSOD), which are all under the control of Nrf2/antioxidant response element (ARE) signaling. Further, mechanistic studies showed that lonchocarpine increases the nuclear translocation and DNA binding of Nrf2 to ARE as well as ARE-mediated transcriptional activities. Moreover, lonchocarpine increased the phosphorylation of AMP-activated protein kinase (AMPK) and three types of mitogen-activated protein kinases (MAPKs). By treating astrocytes with each signaling pathway-specific inhibitor, AMPK, c-jun N-terminal protein kinase (JNK), and p38 MAPK were identified to be involved in lonchocarpine-induced HO-1 expression and ARE-mediated transcriptional activities. Therefore, lonchocarpine may be a potential therapeutic agent for neurodegenerative diseases that are associated with oxidative stress.

Lonchocarpine Increases Nrf2/ARE-Mediated Antioxidant Enzyme Expression by Modulating AMPK and MAPK Signaling in Brain Astrocytes

Jeong, Yeon-Hui,Park, Jin-Sun,Kim, Dong-Hyun,Kim, Hee-Sun The Korean Society of Applied Pharmacology 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.6

Lonchocarpine is a phenylpropanoid compound isolated from Abrus precatorius that has anti-bacterial, anti-inflammatory, antiproliferative, and antiepileptic activities. In the present study, we investigated the antioxidant effects of lonchocarpine in brain glial cells and analyzed its molecular mechanisms. We found that lonchocarpine suppressed reactive oxygen species (ROS) production and cell death in hydrogen peroxide-treated primary astrocytes. In addition, lonchocarpine increased the expression of anti-oxidant enzymes, such as heme oxygenase-1 (HO-1), NAD(P)H:quinone oxidoreductase 1 (NQO1), and manganese superoxide dismutase (MnSOD), which are all under the control of Nrf2/antioxidant response element (ARE) signaling. Further, mechanistic studies showed that lonchocarpine increases the nuclear translocation and DNA binding of Nrf2 to ARE as well as ARE-mediated transcriptional activities. Moreover, lonchocarpine increased the phosphorylation of AMP-activated protein kinase (AMPK) and three types of mitogen-activated protein kinases (MAPKs). By treating astrocytes with each signaling pathway-specific inhibitor, AMPK, c-jun N-terminal protein kinase (JNK), and p38 MAPK were identified to be involved in lonchocarpine-induced HO-1 expression and ARE-mediated transcriptional activities. Therefore, lonchocarpine may be a potential therapeutic agent for neurode-generative diseases that are associated with oxidative stress.

Anti-inflammatory mechanism of lonchocarpine in LPS- or poly(I:C)-induced neuroinflammation

Jeong, Yeon-Hui,Park, Jin-Sun,Kim, Dong-Hyun,Kang, Jihee Lee,Kim, Hee-Sun Elsevier 2017 PHARMACOLOGICAL RESEARCH Vol.119 No.-

<P><B>Abstract</B></P> <P>Neuroinflammation plays an important role in the progression of various neurodegenerative diseases. In this study, we investigated the anti-inflammatory effects of lonchocarpine, a natural compound isolated from <I>Abrus precatorius</I>, under <I>in vitro</I> and <I>in vivo</I> neuroinflammatory conditions induced by challenge with lipopolysaccharide (LPS)- or polyinosinic-polycytidylic acid (poly(I:C)). Lonchocarpine suppressed the expression of iNOS and proinflammatory cytokines in LPS or poly(I:C)-stimulated BV2 microglial cells. These anti-inflammatory effects were verified in brains of mice with systemic inflammation induced by administration of LPS or poly(I:C). Lonchocarpine reduced the number of Iba-1-positive activated microglia, and suppressed the mRNA expression of various proinflammatory markers in the cortex of LPS- or poly(I:C)-injected mice. Molecular mechanistic experiments showed that lonchocarpine inhibited NF-κB activity by reducing the phosphorylation and degradation of IκBα in LPS- or poly(I:C)-stimulated BV2 cells. Analysis of further upstream signaling pathways in LPS-stimulated microglia showed that lonchocarpine inhibited the phosphorylation of IκB kinase and TGFβ-activated kinase 1 (TAK1). Moreover, lonchocarpine suppressed the interaction of myeloid differentiation factor 88 (MyD88) and intereleukin-1 receptor-associated kinase 4 (IRAK4). These data suggest that toll-like receptor 4 downstream signals such as MyD88/IRAK4-TAK1-NF-κB are at least partly involved in the anti-inflammatory mechanism of lonchocarpine in LPS-stimulated microglia. Its strong anti-inflammatory effects may make lonchocarpine an effective preventative drug for neuroinflammatory disorders that are associated with systemic inflammation.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

정상 성인 호흡기능에 대한 들숨 근 강화훈련과 날숨 근 강화 훈련의 효과 비교

이연섭 ( Yeon Seop Lee ),오민영 ( Min Yeong Oh ),박주연 ( Ju Yeon Park ),이대희 ( Dae Hui Lee ),이예진 ( Ye Jin Lee ),정다혜 ( Da Hye Jeong ),홍지연 ( Ji Yeon Hong ),홍하연 ( Ha Yeon Hong ),김현수(교신저자) ( Hyeon Su Kim ) 대한통합의학회 2016 대한통합의학회지 Vol.4 No.1

Purpose : The purpose of this study was to examine the Compare the effects of inspiratory muscle strengthening training and expiratory muscle strengthening training of normal adult respiratory function. Method : In this study, we want to compare the effect of inspiratory muscle strengthening training(n=8) and expiratory muscle strengthening training(n=8) to target the normal adult 16 people. expiratory muscle strengthening training, was 25 minutes of training on the basis of the breathing image program that has been pre-recorded. inspiratory muscle strengthening training, use the power-breathe plus on the measured resistance value, was carried out for 25 minutes. Using the spirometer in order to examine the ability to breathe, FVC, FEV1, FEV1 / FVC, MVV was measured. Result : The results showd that in the breath muscle strengthening training FVC, FEV1, MVV increased statistically significantly. The inspiration muscle strength training FVC, FEV1, MVV was a statistically significant increase, FEV1/FVC decreased. There was no statistically significant difference between. Conclusion : In conclusion, both methods give the result of increasing the effective respiratory function. Inspiratory muscle strengthening training, the function of the lung is very limited to be used when and by us effectively and expiratory muscle strengthening training to increase the capacity of the lung is an effective way that will increase the volume.

MEL-18 loss mediates estrogen receptor-α downregulation and hormone independence

Lee, Jeong-Yeon,Won, Hee-Young,Park, Ji-Hye,Kim, Hye-Yeon,Choi, Hee-Joo,Shin, Dong-Hui,Kang, Ju-Hee,Woo, Jong-Kyu,Oh, Seung-Hyun,Son, Taekwon American Society for Clinical Investigation 2015 The Journal of clinical investigation Vol.125 No.5

지역 농산물의 조단백질과 아미노산 조성 비교

설희경 ( Hui-gyeong Seol ),고희숙 ( Hui-suk Ko ),제희정 ( Hui-jeong Je ),김낙구 ( Nak-ku Kim ),최달연 ( Dal-yeon Choi ),하기정 ( Gi-jeong Ha ) 한국식품영양학회 2017 韓國食品營養學會誌 Vol.30 No.4

This study investigated the crude protein and amino acid contents of local agricultural products widely and specifically grown in Korea, including 25 vegetables and 13 fruits. The crude protein content of vegetables and fruits ranged from 0.46 to 6.53% and 0.29 to 2.23%, respectively. Totally, 17 types of amino acids were found in most samples. The total amino acid content of vegetables and fruits ranged from 457.38 to 9,303.18 mg% and 368.82 to 3,118.75 mg%, respectively. The total amino acid contents of garlic and passion fruit was higher compared to other vagetables and fruits. The calibration curves of the standard components showed good linearity (r2>0.99), except Met (r2=0.989). The limits of LOD and LOQ were in the range 0.034 to 0.991 μg/mL and 0.009 to 0.474 μg/mL, respectively. The results of the study can serve as a fundamental source of information regarding crude protein and amino acids contents in food, for diet planning.

4500 Class Soot Blower PCV 개발

정연호(Jeong Yeon-ho),김영범(Kim Young-bum),정호열(Jeong ho-youl),심영권(Shim young-kwon),최승욱(Choi seung-wook),문재휘(Moon Jae-Hui) 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.11

Steam soot-blower control valves for thermal power plants operate in a uniquely severe service and environment. They must not only frequently modulate flow over a wide range but must also maintain downstream pressure between very close limits at high differential. In addition, they also must function as block valves with tight shutoff every time they close to prevent soot-blower header pressure safety relief valve operation due to system overpressure. Satisfactory operation in these two separate functions is a problem often found in the power industry. Because of poor valve operating experience the soot-blower control valves were recently replaced with valves specifically designed for soot-blower service.

고성 이씨 소장 『해도교거사』의 국어학적 가치

정연정 ( Yeon Jeong Jeong ),천명희 ( Myeong Hui Cheon ) 한국문학언어학회(구 경북어문학회) 2016 어문론총 Vol.68 No.-

이 연구는 『해도교거사』를 소개하고 창작자와 창작시기를 고증하며, 내용에 드러난 국어학적 특성을 고찰하는 데 목적이 있다. 『해도교거사』는 한글로 표기된 내방가사로서 대한민국임시정부 초대 국무령을 지낸 석주 이상룡의 부인인 김우락이 만주로 망명하여 창작한 작품이다. 가사의 말미 부분이 훼손되어 창작자와 창작 시기에 관한 기록이 없지만 가 사의 내용을 토대로 추정이 가능하다. 『해도교거사』는 1911년(辛亥年) 가을 유 하현(柳河縣) 영춘원(永春源)에 정착한 직후에 창작되었으며 따라서 현재까지 알려진 만주 망명지 가사 중 최초의 작품이 된다. 안동에서 출발하여 압록강을 건너는 망명의 경로를 비롯하여 만주 영춘원에 이르는 정착의 과정이 매우 세밀하게 나타나 있어 중요한 사료적 가치가 있다. 또한 작가가 1913~1914년에 창작한 『간운□』, 『조손별서』와 동일하게 망명지에서 독립운동을 함께 한 여성들의 역경과 고난이 잘 드러나 있다. 『해도교거사』에는 개화기 국어의 국어학적 특징이 반영된 표기가 드러나며, 특히 국내외의 지명과 시대 상황을 반영한 어휘는 물론 작가가 60여 년을 생활한 안동지역의 여러 방언이 나타난다. ‘Haedogyogeosa”, which is a ‘gasa’, a form of prose during Joseon dynasty. The summary of the study is as following. Haedogyogeosa is a ‘gasa’ written in Hangeul by a lady. The lady is Kim U-rak, who was the wife of Seokju Yi Sang-ryong. Yi Sang-ryong was the first Gukmuryeong, the head, of the Provisional Government of the Republic of Korea in Shanghai. Kim U-rak wrote this gasa when she was in Manchuria as an exile. The gasa was written immediately after Kim U-rak settled in Yeongchunwon (永春源) in Yuhahyeon (柳河縣), when was the fall of the year 1911 (辛亥年). Therefore, the Haedogyogeosa is the earliest one among the gasa’s known to have been written by Koreans in Manchuria in exile. Kim U-rak also wrote other gasa’s such as Ganwunsa and Jyosonbyeolseo in 1913 and 1914. Like these two gasa’s, the Haedogyogeosa describes the hardship and adversity suffered by the ladies, who participated in independence movement in exile. The gasa is an important one because it has historical value by describing the route of exile and the process of settlement until they arrived at Yeongchunwon in very minute detail. There are Hangeul writings which reflect the characteristics of Korean linguistics at the late 19th century. The geographical names of Korean and overseas and the vocabularies reflected the time also include various dialects of Andong area, where Kim U-rak lived more than 60 years.