Correlation between DNA methylation and Thymic Stromal Lymphopoietin expression in asthmatic airway epithelial cells

Yan‑Li Li,Xi‑Qian Xing,Yi Xiao,Yan‑Hong Liu,Yu‑Shan Zhou,Min Zhuang,Chao‑Qian Li 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: The overexpression of TSLP and DNA methylation in asthma were both risk factors the relationship was not clear. Objective: This study aimed to investigate the relationship between methylation status of TSLP promoter and mRNA/protein expression in asthmatic airway epithelial cells. Methods: Human bronchial epithelial cells were cultured in vitro and divided into: Control group, treated with PBS, model group, sensitized with LPS (10 μg/mL) for 12 h (37 °C, 5% CO2). Other groups were cultured with the pCMV3 plasmid (M + NC/pCMV), pGPH1 plasmid (M + NC/pGPH), DNMT1/pCMV3 plasmid (M + DNMT1/pCMV), and DNMT1/pGPH1 plasmid (M + DNMT1/pGPH) for 48 h. The expression of DNA methyltransferase 1 and TSLP were measured using real-time PCR and western blotting. Results: Compared with the control group, TSLP mRNA (1.00 ± 0.00 vs. 2.82 ± 0.81 vs. 1, P < 0.001) and protein (1.07 ± 0.04 vs. 1.46 ± 0.11, P < 0.01) were significantly greater, and the methylation of promoter was lower (92.75 ± 1.26 vs. 58.57 ± 3.34, P < 0.05) in the model group. Compared with the model group, TSLP mRNA (2.82 ± 0.81 vs. 1.17 ± 0.10, P < 0.001) decreased, but TSLP promoter methylation increased (58.57 ± 3.34 vs. 92.58 ± 7.30, P < 0.05) in M + DNMT1/pCMV. TSLP mRNA and protein were higher (2.82 ± 0.81 vs. 5.32 ± 0.21, P < 0.001; 1.46 ± 0.11 vs. 1.94 ± 0.11, respectively, P < 0.01), TSLP promoter methylation was lower (58.57 ± 3.34 vs. 33.57 ± 4.29, P < 0.05) in M + DNMT1/pGPH. Conclusions: Overexpression of TSLP in asthmatic airway epithelial cells may be regulated by DNA demethylation.

LPL gene Pvu II polymorphism and hypertriglycer-idemia: a meta-analysis involving 1,640 subjects

( Yan-yan Li ),( Yan-hong Zhou ),( Ge Gong ),( Hong-yu Geng ),( Xin-xing Yang ),( Xiang-ming Wang ),( Chuan-wei Zhou ),( Jian Xu ),( Yun Qian ) 대한내과학회 2017 The Korean Journal of Internal Medicine Vol.32 No.6

Background/Aims: Although lipoprotein lipase (LPL) gene Pvu II polymorphism has been associated with an increased risk of hypertriglyceridemia (HT), there is no clear consensus within the scientific community. Methods: A meta-analysis of 1,640 subjects from six individual studies was conducted to better elucidate the potential relationship between the LPL gene Pvu II polymorphism and HT within the Chinese population. Pooled odds ratios (ORs) and their corresponding 95% confidence intervals (CIs) were evaluated by using fixed effect models. Results: Our analysis indicated a significant association between LPL gene Pvu II polymorphism and HT within the Chinese population under allelic (OR, 1.550; 95% CI, 1.320 to 1.830; p = 1.158 × 10^-7), recessive (OR, 0.540; 95% CI, 0.390 to 0.750; p = 0.0002), dominant (OR, 1.889; 95% CI, 1.501 to 2.377; p = 5.960 × 10^-8), homozygous (OR, 2.167; 95% CI, 1.531 to 3.067; p = 1.242 × 10^-5), heterozygous (OR, 1.810; 95% CI, 1.419 to 2.309; p = 1.842 × 10^-6), and additive genetic models (OR, 1.553; 95% CI, 1.320 to 1.828; p = 1.158 × 10^-7). Conclusions: Because LPL gene Pvu II restriction fragment length polymorphism polymorphism was associated with an elevated risk of HT, the P+ allele carriers of the LPL gene might be predisposed to HT.

Electrically conductive thin-film color filters made of single-material indium-tin-oxide

Yan, Xing,Mont, Frank W.,Poxson, David J.,Cho, Jaehee,Schubert, E. Fred,Kim, Min-Ho,Sone, Cheolsoo American Institute of Physics 2011 JOURNAL OF APPLIED PHYSICS - Vol.109 No.10

NP+好-V與NP+容易-V的對比分析

( Xing-yan Guo ) 중국어문연구회 2015 中國語文論叢 Vol.0 No.72

In modern Chinese, “Hao” of “NP+hao-V” means “easy”, and “NP+hao-V” is used more frequently than “NP+rongyi-V”. “NP+hao-V” and “NP+rongyi-V” have many differences in the sentence meaning, syntactic component, the degree of construction. On the one hand, the construction degree of “NP+hao-V is very high but it has certain restrictions in“V . NP” choice. On the other hand, the construction degree of “NP+rongyi-V” is not high but it doesn’t have any restrictions to “V” or “V-NP”. There are many sentences that can``t be expressed with “NP+hao-V”, but could be used “NP+rongyi-V” instead, at the meantime the term “NP+rongyi-V” doesn``t express “it``s easy to do something”, which means “high possibility”, the meaning of the sentence is changed. The term “NP+hao-V” is mainly used in spoken English while “NP+rongyi-V” can be used both in spoken language and written language.

ON THE RECURSIVE SEQUENCE X_{n+1} = $\alpha$ - (X_n/X_n-1)

YAN, XING XUE,LI, WAN TONG,ZHAO, ZHU 한국전산응용수학회 2005 Journal of applied mathematics & informatics Vol.17 No.1

We study the global asymptotic stability, global attractivity, boundedness character, and periodic nature of all positive solutions and all negative solutions of the difference equation $$x_{n+1}\;=\;{\alpha}-{\frac{x_{n-1}}{x_{n}},\;n=0,1,\;{\cdots}$$, where ${\alpha}\;\in\; R$ is a real number, and the initial conditions $x_{-1},\;x_0$ are arbitrary real numbers.

Recyclable Fe3O4/Au Nanocomopsites for Oxidation Degradation of Methylene Blue in Near Neutral Solution

Yan Xing,Xiao-Hui Bai,Ming-Li Peng,Xiang-Rong Ma,Norbert Buske,Ya-Li Cui 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.10

Fe3O4/Au nanocomopsites (Fe3O4/Au NPs) with much improved catalytic activity were successfully fabricated through a simple seed growth method in aqueous solution. The petal-like structure, high saturation magnetization, the negatively charged sodium citrate-stabilized Fe3O4/Au NPs was characterized by transmission electron microscopy (TEM), X-ray diffractometer (XRD), dynamic light scattering (DLS) and vibrating sample magnetometry (VSM). The activated-H2O2 ability of Fe3O4/Au NPs was evaluated by using methylene blue (MB) as a cationic phenothiazines dye to be degraded in near neutral solution. The results showed Fe3O4/Au NPs removed over 95% MB from an aqueous solution within 60 min under the optimum conditions. The apparent rate constant of Fe3O4/Au NPs was 10.8 x 10 -2 min -1 which was 43.2 and 8.3 times higher than pure Fe3O4 (2.5 x 10 -3 min -1) and Au (1.3 x 10 -2 min -1) NPs. The enhanced catalytic activity and increased oxidation rate constant probably owing to the synergistic effect between Fe3O4 and Au NPs to activate H2O2 generate a large amount of strong oxidizing species, such as ·OH. In addition, nanocrystalline structure of Fe3O4/Au NPs was also very important to the peroxidase-like effect, especially the interaction interface between Fe3O4 and Au NPs. Moreover, Fe3O4/Au NPs was stable and could be regenerated and reused for at least five cycles.

Enhanced Omnidirectional Photovoltaic Performance of Solar Cells Using Multiple‐Discrete‐Layer Tailored‐ and Low‐Refractive Index Anti‐Reflection Coatings

Yan, Xing,Poxson, David J.,Cho, Jaehee,Welser, Roger E.,Sood, Ashok K.,Kim, Jong Kyu,Schubert, E. Fred WILEY‐VCH Verlag 2013 Advanced Functional Materials Vol.23 No.5

<P><B>Abstract</B></P><P>An optimized four‐layer tailored‐ and low‐refractive index anti‐reflection (AR) coating on an inverted metamorphic (IMM) triple‐junction solar cell device is demonstrated. Due to an excellent refractive index matching with the ambient air by using tailored‐ and low‐refractive index nanoporous SiO<SUB>2</SUB> layers and owing to a multiple‐discrete‐layer design of the AR coating optimized by a genetic algorithm, such a four‐layer AR coating shows excellent broadband and omnidirectional AR characteristics and significantly enhances the omnidirectional photovoltaic performance of IMM solar cell devices. Comparing the photovoltaic performance of an IMM solar cell device with the four‐layer AR coating and an IMM solar cell with the conventional SiO<SUB>2</SUB>/TiO<SUB>2</SUB> double layer AR coating, the four‐layer AR coating achieves an angle‐of‐incidence (AOI) averaged short‐circuit current density, <I>J</I><SUB>SC</SUB>, enhancement of 34.4%, whereas the conventional double layer AR coating only achieves an AOI‐averaged <I>J</I><SUB>SC</SUB> enhancement of 25.3%. The measured reflectance reduction and omnidirectional photovoltaic performance enhancement of the four‐layer AR coating are to our knowledge, the largest ever reported in the literature of solar cell devices.</P>

Analysis on Electric Field Based on Three Dimensional Atmospheric Electric Field Apparatus

Xing, Hong-yan,He, Gui-xian,Ji, Xin-yuan The Korean Institute of Electrical Engineers 2018 Journal of Electrical Engineering & Technology Vol.13 No.4

As a key component of lighting location system (LLS) for lightning warning, the atmospheric electric field measuring is required to have high accuracy. The Conventional methods of the existent electric field measurement meter can only detect the vertical component of the atmospheric electric field, which cannot acquire the realistic electric field in the thunderstorm. This paper proposed a three dimensional (3D) electric field system for atmospheric electric field measurement, which is capable of three orthogonal directions in X, Y, Z, measuring. By analyzing the relationship between the electric field and the relative permittivity of ground surface, the permittivity is calculated, and an efficiency 3D measurement model is derived. On this basis, a three-dimensional electric field sensor and a permittivity sensor are adopted to detect the spatial electric field. Moreover, the elevation and azimuth of the detected target are calculated, which reveal the location information of the target. Experimental results show that the proposed 3D electric field meter has satisfactory sensitivity to the three components of electric field. Additionally, several observation results in the fair and thunderstorm weather have been presented.

On the recursive sequence$x_{n+1}=\alpha-(x_n/x_{n-1})$

Xing-Xue Yan,Wan-Tong Li,Zhu Zhao 한국전산응용수학회 2005 Journal of applied mathematics & informatics Vol.17 No.1-2

We study the global asymptotic stability, global attractivity, boundedness character, and periodic nature of all positive solutions and all negative solutions of the difference equation xn+1 = − xn xn−1 , n = 0, 1, · · · , where 2 R is a real number, and the initial conditions x−1, x0 are arbitrary real numbers

Effect of a Novel Intracycle Motion Correction Algorithm on Dual-Energy Spectral Coronary CT Angiography: A Study with Pulsating Coronary Artery Phantom at High Heart Rates

Yan Xing,Yuan Zhao,Ning Guo,Cun-Xue Pan,Gulina Azati,Yan-Wei Wang,Wen-Ya Liu 대한영상의학회 2017 Korean Journal of Radiology Vol.18 No.6

Objective: Using a pulsating coronary artery phantom at high heart rate settings, we investigated the efficacy of a motion correction algorithm (MCA) to improve the image quality in dual-energy spectral coronary CT angiography (CCTA). Materials and Methods: Coronary flow phantoms were scanned at heart rates of 60−100 beats/min at 10-beats/min increments, using dual-energy spectral CT mode. Virtual monochromatic images were reconstructed from 50 to 90 keV at 10-keV increments. Two blinded observers assessed image quality using a 4-point Likert Scale (1 = non-diagnostic, 4 = excellent) and the fraction of interpretable segments using MCA versus conventional algorithm (CA). Comparison of variables was performed with the Wilcoxon rank sum test and McNemar test. Results: At heart rates of 70, 80, 90, and 100 beats/min, images with MCA were rated as higher image scores compared to those with CA on monochromatic levels of 50, 60, and 70 keV (each p < 0.05). Meanwhile, at a heart rate of 90 beats/min, image interpretability was improved by MCA at a monochromatic level of 60 keV (p < 0.05) and 70 keV (p < 0.05). At a heart rate of 100 beats/min, image interpretability was improved by MCA at monochromatic levels of 50 keV (from 69.4% to 86.1%, p < 0.05), 60 keV (from 55.6% to 83.3%, p < 0.05) and 70 keV (from 33.3% to 69.3%, p < 0.05). Conclusion: Low-keV monochromatic images combined with MCA improves image quality and image interpretability in CCTAs at high heart rates.