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겨울철 자연(통풍)냉각과 상부 쿨링시스템을 이용한 남부 지역의 벼 고품질 유지를 위한 저온저장기술 개발
녕효봉,사공문,함택모,조성찬 忠北大學校 農業科學硏究所 2012 農業科學硏究 Vol.28 No.2
The purpose of this study is to investigate the storage characteristics of rough rice in a low temperature storage and dry bin using cooling system. The results were compared with the storage method of ambient temperature bin. The rough rice storage characteristics were evaluated from February to August 2010, using a storage and dry bin of 500 ton capacity. The results were as followings: Storage and dry bin using cooling system maintained the rice temperature less than 15oC over the storage period. The rough rice stored in the ambient temperature bin showed higher moisture content and lower germination rate than the rough rice stored in the bin using cooling system. The creaked rate and acid value of brown rice in the cooling bin storage increased less than the ambient temperature bin storage. The result indicates that storage bin using winter cold air and cooling system showed better maintaining germination of rice, less change of physiological activities and cracked kernel, and better maintaining of rice quality, comparing the ambient temperature bin
( He Nan Li ),( Xiao Huan Guo ),( Lu Ning Shao ),( Markus Plate ),( Xiao Ning Mo ),( Yu Wang ),( Wen Ling Han ) 한국생화학분자생물학회 (구 한국생화학회) 2010 BMB Reports Vol.43 No.3
The CKLF-like MARVEL transmembrane domain-containing family (CMTM) is a novel family of proteins linking classical chemokines and the transmembrane 4 superfamily (TM4SF). Our earlier studies indicated several CMTM members (such as CKLF1 and CMTM2) have a secreted form. This is the first report of the secreted form of CMTM5-v1, the major RNA splicing form of CMTM5, which is produced as small vesicles (<100 nm diameter) and floats at a peak density of 1.19 g/ml on continuous sucrose gradients. CMTM5-v1 has no obvious co-localization with CD63 or Golgi complex. In addition, brefeldin A but not wortmannin can inhibit the secretion of CMTM5-v1. Our results suggest that CMTM5-v1 might be secreted via a different vesicle-mediated secretory pathway, which will be helpful for the studies of vesicle-mediated secretion and MARVEL domain-containing proteins. [BMB reports 2010; 43(3): 182-187]
Geft is dispensable for the development of the second heart field
( Xiong Wei Fan ),( Ning Hou ),( Kai Ji Fan ),( Jia Jia Yuan ),( Xiao Yang Mo ),( Yun Deng ),( Yong Qi Wan ),( Yan Teng ),( Xiao Yang ),( Xius Han Wu ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.3
Geft is a guanine nucleotide exchange factor, which can specifically activate Rho family of small GTPase by catalyzing the exchange of bound GDP for GTP. Geft is highly expressed in the excitable tissue as heart and skeletal muscle and plays important roles in many cellular processes, such as cell proliferation, migration, and cell fate decision. However, the in vivo role of Geft remains unknown. Here, we generated a Geft conditional knockout mouse by flanking exons 5-17 of Geft with loxP sites. Cre-mediated deletion of the Geft gene in heart using Mef2c-Cre transgenic mice resulted in a dramatic decrease of Geft expression. Geft knockout mice develop normally and exhibit no discernable phenotype, suggesting Geft is dispensable for the development of the second heart field in mouse. The Geft conditional knockout mouse will be a valuable genetic tool for uncovering the in vivo roles of Geft during development and in adult homeostasis. (BMB reports 2012; 45(3): 153-158)