http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Sequential Polyadenylation to Enable Alternative mRNA 3’ End Formation
Xiang-Dong Fu,Yajing Hao,Ting Cai,Chang Liu,Xuan Zhang 한국분자세포생물학회 2023 Molecules and cells Vol.46 No.1
In eukaryotic cells, a key RNA processing step to generate mature mRNA is the coupled reaction for cleavage and polyadenylation (CPA) at the 3′ end of individual transcripts. Many transcripts are alternatively polyadenylated (APA) to produce mRNAs with different 3′ ends that may either alter protein coding sequence (CDS-APA) or create different lengths of 3′UTR (tandem-APA). As the CPA reaction is intimately associated with transcriptional termination, it has been widely assumed that APA is regulated cotranscriptionally. Isoforms terminated at different regions may have distinct RNA stability under different conditions, thus altering the ratio of APA isoforms. Such differential impacts on different isoforms have been considered as post-transcriptional APA, but strictly speaking, this can only be considered “apparent” APA, as the choice is not made during the CPA reaction. Interestingly, a recent study reveals sequential APA as a new mechanism for post-transcriptional APA. This minireview will focus on this new mechanism to provide insights into various documented regulatory paradigms.
Fu Li,Yongchun Dong,Weimin Kang,Bo-wen Cheng,Xiang Qu,Guixin Cui 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.12
Two transition metal ions (Fe3+ and Cu2+) and a rare earth metal ion (Ce3+) were selected to coordinate with amidoximated polyacrylonitrile (PAN) nanofibrous membrane for preparing three metal modified PAN nanofibrous membrane complexes (M-AO-n-PANs, M = Fe, Cu, or Ce) as the heterogeneous Fenton catalysts for the dye degradation in water under visible irradiation. The coordination kinetics of three metal ions with modified PAN nanofibrous membranes was studied and the catalytic properties of the resulting complexes were also compared. The results indicated that increasing metal ion concentrations in solution or higher coordination temperature led to a significant increase in metal content, particularly in Fe and Cu contents of the complexes. Their coordination process could be described using Langmuir isotherm and pseudo-second-order kinetic equations. Moreover, Fe-AO-n-PAN had the best photocatalytic efficiency for the dye degradation in acidic medium, but a lower photocatalytic activity than Cu-AO-n-PAN in alkali medium.
Mineralization of calcium phosphate in reverse microemulsion
Xiang-Dong Kong,Xiao-Dan Sun,Jun-Biao Lu,Fu-Zhai Cui 한국물리학회 2005 Current Applied Physics Vol.5 No.5
In the present study, reverse microemulsion was prepared to regulate the mineralization of calcium phosphate. Calcium chlorideand sodium hydrogen phosphate aqueous solution were, respectively, dropped into the mixture of Span 80, Tween 80, andn-heptaneto get two kinds of emulsions.n-Buranol was used to adjust the emulsions to transparent state. Calcium phosphate was prepared byadding phosphate microemulsion to Calcium microemulsion. Heating method was used to induce the phase separation. After beingwashed with acetone and ethanol, the mineralized deposition were studied with scanning electron microscopy (SEM), X-ray dirac-tion (XRD) and transmission electron microscopy (TEM). The results indicated that the minerals in the deposition were mainlymonetite (DCPA) and the nanosized needle-like DCPA exhibited the preferential orientation along 200 direction..
Liuxin Xiang,Yuxian Xia,Ying-Fan Cai,Jijun Liu,Xiaohong He,Quan Sun,Xiaoyan Wang,Yuyin Fu,Yonghong Fan,Daiwen Dong,Guanfan Zhou,Jinjuan Shen,Yihua Liu 한국식물학회 2013 Journal of Plant Biology Vol.56 No.3
The first tuber mustard calmodulin-like (CML) gene BjAAR1 (Brassica juncea var. tumida Tsen et Lee Abiotic stress and Abscisic acid (ABA) Responsive gene 1) was cloned and characterized. The protein encoded by BjAAR1 contains four predicted Ca2+ binding sites (EF-hand motif) and its recombinant protein can bind Ca2+ in vitro. qRT-PCR showed that the expression level of BjAAR1 was rather high in non-swollen stem of tuber mustard and largely reduced in swollen stem. Expression of BjAAR1 enhanced ABA- and stress-induced gene expression in Arabidopsis (Arabidopsis thaliana). Transgenic plants also exhibited hypersensitivity to NaCl, mannitol, and ABA during the seed germination and post-germination stages. ABA biosynthesis inhibitor, norflurazon (NF), rescued hypersensitivity phenotype of transgenic plants to NaCl and mannitol, indicating that BjAAR1 functions in multiple abiotic stresses response through ABA-dependent process.
Zhi-Fu Guo,Xiang-Yu Long,Pan Dong,Yu-Ming Wei,Li-Ping Bai,Xiao-Xuan Dang,Hao-Lei Wan,Li-Jun Zhang,You-Liang Zheng 한국유전학회 2011 Genes & Genomics Vol.33 No.2
The α-gliadins from Crithopsis delileana (Schult) Roshev (2n=2x=14, KK) were investigated by Acid polyacrylamide gel electrophoresis (A-PAGE) analysis. It was indicated that the electrophoresis mobility of gliadins from C.delileana had obvious difference with those from common wheat in α, γand ω region. Using primers designed from published sequences of α-gliadin genes, three α-gliadin genes were isolated from C. delileana, which were designated as gli-ka1,gli-ka2 and gli-ka3, respectively. Two in-frame stop codons were found in the coding sequences of gli-ka3, indicating that gli-ka3 could be a pseudogene. The gli-ka2 was a gliadin with an odd number of cysteines, resulting from a non-synonymous mutation. This change might lead to the interactive behavior of gli-ka2. Three α-gliadin genes of C. delileana had the similar but not identical primary structures to the corresponding gene sequences from other wheat related species. By the alignment of α-gliadin genes from Triticeae,phylogenetic analysis indicated that three α-gliadin genes of C. delileana clustered together with all α-gliadin genes from Ee genome of Lophopyrum elongatum by an interior paralleled branch.
MicroRNA Expression Profile Analysis Reveals Diagnostic Biomarker for Human Prostate Cancer
Liu, Dong-Fu,Wu, Ji-Tao,Wang, Jian-Ming,Liu, Qing-Zuo,Gao, Zhen-Li,Liu, Yun-Xiang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7
Prostate cancer is a highly prevalent disease in older men of the western world. MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression via posttranscriptional inhibition of protein synthesis. To identify the diagnostic potential of miRNAs in prostate cancer, we downloaded the miRNA expression profile of prostate cancer from the GEO database and analysed the differentially expressed miRNAs (DE-miRNAs) in prostate cancerous tissue compared to non-cancerous tissue. Then, the targets of these DE-miRNAs were extracted from the database and mapped to the STRING and KEGG databases for network construction and pathway enrichment analysis. We identified a total of 16 miRNAs that showed a significant differential expression in cancer samples. A total of 9 target genes corresponding to 3 DE-miRNAs were obtained. After network and pathway enrichment analysis, we finally demonstrated that miR-20 appears to play an important role in the regulation of prostate cancer onset. MiR-20 as single biomarker or in combination could be useful in the diagnosis of prostate cancer. We anticipate our study could provide the groundwork for further experiments.
Tian-Liang Fu,Xiang-Tao Deng,Guo-Huai Liu,Zhao-Dong Wang,Guo-Dong Wang 한국정밀공학회 2016 International Journal of Precision Engineering and Vol.17 No.11
The quenching temperature drop curve for Q345B steel plate with 84 mm and 170 mm thickness was tested to analyze the distributing regularities and influencing factors of cooling speed for ultra-thick steel plate during the jet impinging and quenching process. The influences for temperature drop, temperature gradient and cooling speed were analyzed under the conditions of 60~100 m3/h water amount, 0.4~1.0 MPa water pressure, transient switching of quenching mode and the distribution of heat exchanger. Threedimensional heat anti transfer model, surface heat transfer coefficient model and thermal physical parameter model were built up by finite element and optimization. The results showed that the deviation of calculated and measured values was less than 4% for temperature drop curve model. The cooling speed of vertical section for 84 mm-thick steel plate was approximately proportional to surface heat transfer coefficient. The influence of surface heat transfer to cooling speed became weak when the thickness was increased. The influences of temperature effect when switching different quenching modes and temperature gradient of vertical section to cooling speed were stronger. The minimum value of cooling speed was about 1.0~1.8oC/s, between H/6 and H/3 region. These data provide the key information for increasing the cooling speed and uniformity.
Wang, Chang-Dong,Yuan, Cheng-Fu,Bu, You-Quan,Wu, Xiang-Mei,Wan, Jin-Yuan,Zhang, Li,Hu, Ning,Liu, Xian-Jun,Zu, Yong,Liu, Ge-Li,Song, Fang-Zhou Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2
Fangchinoline (Fan) inhibits cell proliferation and induces apoptosis in several cancer cell lines. The effects of Fan on cell growth and proliferation in breast cancer cells remain to be elucidated. Here, we show that Fan inhibited cell proliferation in the MDA-MB-231 breast cancer cell line through suppression of the AKT/Gsk-3beta/cyclin D1 signaling pathway. Furthermore, Fan induced apoptosis by increasing the expression of Bax (relative to Bcl-2), active caspase 3 and cytochrome-c. Fan significantly inhibited cell proliferation of MDA-MB-231 cells in a concentration and time dependent manner as determined by MTT assay. Flow cytometry analysis demonstrated that Fan treatment of MDA-MB-231 cells resulted in cell cycle arrest at the G1 phase, which correlated with apparent downregulation of both mRNA and protein levels of both PCNA and cyclin D1. Further analysis demonstrated that Fan decreased the phosphorylation of AKT and GSK-3beta. In addition, Fan up-regulated active caspase3, cytochrome-c protein levels and the ratio of Bax/Bcl-2, accompanied by apoptosis. Taken together, these results suggest that Fan is a potential natural product for the treatment of breast cancer.