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간세포암과 하대정맥 막성폐쇄의 치료 후 호전된 백혈구파괴혈관염 1예
이준엽 ( Jun Yeob Lee1 ),이주원 ( Ju Won Lee1 ),이진욱 ( Jin Wook Lee ),박현준 ( Hyun Joon Park ),장국환 ( Gook Hwan Jang ),김다정 ( Da Jung Kim ),김선민 ( Sun Min Kim ),한병훈 ( Byung Hoon Han ),정규식 ( Gyoo Sik Jung ),김근태 ( 대한류마티스학회 2015 대한류마티스학회지 Vol.22 No.5
저자들은 하지 자색반과 고환 통증으로 내원한 알코올성 간경화증 환자에서 LCV의 원인 감별하는 과정에서 HCC와 하대정맥 막성폐쇄를 진단하고 이에 대한 치료 후 LCV의 호전된 증례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Vasculitis is a heterogeneous group of diseases that destroy blood vessel walls by inflammation. Approximately half of vasculitis cases are idiopathic, but sometimes associated with genetic factors, medicines, chronic infection, autoimmune diseases, and malignancies. Although the mechanism remains unclear, vasculitis secondary to malignancy, also known as paraneoplastic vasculitis, has been reported. It is generally associated with hematologic malignancies rather than solid malignancies and commonly presents as leukocytoclastic vasculitis or polyarteritis nodosa. We experienced a case of leukocytoclastic vasculitis in a patient with hepatocellular carcinoma and membranous obstruction of the inferior vena cava. Here, we report this case with a brief review of literature. (J Rheum Dis 2015;22:322-326)
동절기 단열갱폼으로 인한 경제성 효과 및 투입 원가 분석 연구
원준연,이영도,남경용,Won, Joon-Yuen,Lee, Young-Do,Nam, Kyung-Yong 한국건축시공학회 2018 한국건축시공학회지 Vol.18 No.4
This paper verifies the superiority of warming work in winter by applying the insulation gang-form to the apartment housing site and analyzes the economic feasibility of the application. According to the experimental results, the actual cost of warming work was about 52 million won less than planned, and 160 million won less than the existing average.(Note - The cost of gang form material increased from 260 million won to 310 million won after the change) As a result, the construction cost could be reduced by about 110 million won. As the costs of warming work can change depending on the number of floors, the building number, and the area of each site, it is deemed necessary to conduct a thorough review in advance at the site where the cost of warming work is to be applied. 본 논문은 단열갱폼의 공동주택현장 적용을 통해 단열갱폼 동절기 보양우수성을 확인하고, 현장 적용에 따른 경제적 타당성을 분석하고 있다. 실험결과에 따르면 실제 보양투입 비용은 사전계획대비 약 5천 2백만 원을 절감하였으며, 기존 일반갱폼대비 약 1억 6천만 원을 절감하였다.(참고 - 변경전 갱폼 자재비는 2억 6천만 원에서 변경 후 3억 1천만 원으로 약 5천만 원 증가함) 결과적으로 단열갱폼으로 변경하여 약 1.1억 원 가량의 공사비를 절감할 수 있었다. 현장마다 층수, 동수, 면적이 달라짐에 따라 보양공사비가 변경될 수 있으니 단열갱폼 적용 예정인 현장에서는 사전에 충분한 검토가 이루어져야 할 것으로 판단된다.
Down-regulation of survivin suppresses uro-plasminogen activator through transcription factor JunB
Lee, Kyung-Hee,Choi, Eun-Young,Koh, Sung-Ae,Kim, Min-Kyoung,Kim, Kyeong-Ok,Lee, Si-Hyung,Jang, Byung-Ik,Kim, Se-Won,Kim, Sang-Woon,Song, Sun-Kyo,Choi, Joon-Hyuk,Kim, Jae-Ryong Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.9
Survivin, a member of the inhibitors of apoptosis protein family, is expressed during development and in various human cancers. However, the clinical relevance of survivin in cancer is still a matter of debate. Genes induced by hepatocyte growth factor (HGF) were screened using cDNA microarray technology in the stomach cancer cell lines, NUGC3 and MKN28. The levels of JunB, survivin, and uro-plasminogen activator (uPA) were up-regulated in cells treated with HGF in a dose-dependent manner. HGF-induced up regulation of JunB, survivin, and uPA was inhibited by pre-treatment with a MEK inhibitor (PD 98059). HGF-induced up-regulation of uPA was repressed by survivin knockdown. HGF enhanced the binding activity of JunB to the survivin promoter in control cells, but not in the JunB-shRNA cells. Transfection with survivin- shRNA resulted in a decrement of cell proliferation, as determined with MTT assays. In an $in$ $vitro$ invasion assay, significantly fewer cells transfected with survivin shRNA than control cells were able to invade across a Matrigel membrane barrier. In conclusion, survivin appeared to play an important role in the up-regulation of uPA induced by HGF $via$ JunB and might contribute to HGF-mediated tumor invasion and metastasis, which may serve as a promising target for gastric cancer therapy.
Efficacy of Intracytoplasmic Sperm Injection for Leukocytospermia
Lee, Hyang-Heun,Lee, Hoi-Chang,Ko, Duck-Sung,Park, Won-Il,Kim, Seung-Samuel,Lim, Hee-Joung,Bae, Hyung-Joon,Moon, Hi-Joo,Kang, Hee-Gyoo The Korean Society for Biomedical Laboratory Scien 2004 Journal of biomedical laboratory sciences Vol.10 No.1
White blood cells (WBCs) are present in most human ejaculates, but abnormally high concentration of seminal leukocytes may reflect an underlying pathological condition. The World Health Organization (WHO) has defined leukocytospermia as status of more than $10^6$ WBC/mL of semen. The purpose of this study was firstly, to compare the outcomes between conventional IVF and ICSI in leukocytospermia, and secondly, to investigate whether ICSI may be an alternation treatment for patients with leukocytospermia. Total 346 cycles of conventional IVF and ICSI candidates underwent IVF cycles at Eulji Hospital Infertility Clinic. Semen Parameters including concentration, motility, morphology of spermatozoa and concentration of leukocytes were assessed from the raw ejaculates. There was no difference in sperm concentration, motility and morphology. The rates of fertilization and good embryo development from ICSI were significantly higher than those from conventional IVF in leukocytospermia (60.4% & 32.5%, respectively for ICSI group and 44.4% & 28.5%, respectively for IVF group, P<0.00l). The pregnancy rate after ICSI was also higher than that from conventional IVF (34.0% vs 29.1 %, P<0.05). These results indicate that the presence of seminal leukocyes (> 1$\times10^6$ WBC/mL of semen) is adversely related with fertilization, embryo development and pregnancy rate. Therfore the measurement of seminal leukocytes in routine semen analysis appears to be of prognostic value with regard to male fertilizing potential. In conclusion, it is suggested that ICSI is an alternative choice of treatment for patients with leukocytospermia.
Lee, Dong-Kyu,Long, Nguyen Phuoc,Jung, Juwon,Kim, Tae Joon,Na, Euiyeon,Kang, Yun Pyo,Kwon, Sung Won,Jang, Jiho Elsevier 2019 Biochemical and biophysical research communication Vol.508 No.2
<P><B>Abstract</B></P> <P>Precise pathophysiology with respect to the phenotypic variations and severity of X-ALD, specifically between adrenomyeloneuropathy (AMN) and childhood cerebral adrenoleukodystrophy (CCALD), has not been fully discovered. Herein, a systematic analysis using multi-layered lipidomics and transcriptomics was conducted to elucidate distinctive metabolic biosignatures among healthy control, AMN, and CCALD. Significant alterations regarding the accumulation of very long chain fatty acids were found in various lipid species such as phospholipids, glycerolipids, and sphingolipids. Remarkably, TG and CER that are physiologically essential were markedly down-regulated in CCALD than AMN. Transcriptomic analysis further supported the robustness of our findings by providing valuable information on the gene expressions of the regulatory factors. For instance, regulators of sphingolipid catabolism (SMPD1, CERK, and SPHK1) and TG anabolism (GPAM, GPAT2, and MBOAT2) were more up-regulated in AMN than in CCALD. These observations, among others, were in line with the recognized alterations of the associated lipidomes. In conclusion, the homeostatic imbalance of the complex lipid networks may be pathogenically important in X-ALD and the particular dysregulations of TG and CER may further influence the severity of CCALD among X-ALD patients.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lipid profiling reveals distinctive biosignatures of X-linked adrenoleukodystrophy. </LI> <LI> Adrenomyeloneuropathy, mild type, activates the anabolism of sphingolipid pathway. </LI> <LI> Childhood cerebral adrenoleukodystrophy lacks the synthesis of triacylglycerol. </LI> <LI> The collapse of the lipid homeostasis is related to the severity of disease. </LI> </UL> </P>
Intragenic CpG islands play important roles in bivalent chromatin assembly of developmental genes
Lee, Sun-Min,Lee, Jungwoo,Noh, Kyung-Min,Choi, Won-Young,Jeon, Sejin,Oh, Goo Taeg,Kim-Ha, Jeongsil,Jin, Yoonhee,Cho, Seung-Woo,Kim, Young-Joon National Academy of Sciences 2017 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.114 No.10
<P>CpG, 5'-C-phosphate-G-3', islands (CGIs) have long been known for their association with enhancers, silencers, and promoters, and for their epigenetic signatures. They are maintained in embryonic stem cells (ESCs) in a poised but inactive state via the formation of bivalent chromatin containing both active and repressive marks. CGIs also occur within coding sequences, where their functional role has remained obscure. Intragenic CGIs (iCGIs) are largely absent from housekeeping genes, but they are found in all genes associated with organ development and cell lineage control. In this paper, we investigated the epigenetic status of iCGIs and found that they too reside in bivalent chromatin in ESCs. Cell type-specific DNA methylation of iCGIs in differentiated cells was linked to the loss of both the H3K4me3 and H3K27me3 marks, and disruption of physical interaction with promoter regions, resulting in transcriptional activation of key regulators of differentiation such as PAXs, HOXs, and WNTs. The differential epigenetic modification of iCGIs appears to be mediated by cell type-specific transcription factors distinct from those bound by promoter, and these transcription factors may be involved in the hypermethylation of iCGIs upon cell differentiation. iCGIs thus play a key role in the cell type-specific regulation of transcription.</P>
Lee, Ho-Joon,Son, Myung Jin,Ahn, Jiwon,Oh, Soo Jin,Lee, Mihee,Kim, Ansoon,Jeung, Yun-Ji,Kim, Han-Gyeul,Won, Misun,Lim, Jung Hwa,Kim, Nam-Soon,Jung, Cho-Rock,Chung, Kyung-Sook Elsevier 2017 Acta Biomaterialia: structure-property-function re Vol.64 No.-
<P><B>Abstract</B></P> <P>Current <I>in vitro</I> liver models provide three-dimensional (3-D) microenvironments in combination with tissue engineering technology and can perform more accurate <I>in vivo</I> mimicry than two-dimensional models. However, a human cell-based, functionally mature liver model is still desired, which would provide an alternative to animal experiments and resolve low-prediction issues on species differences. Here, we prepared hybrid hydrogels of varying elasticity and compared them with a normal liver, to develop a more mature liver model that preserves liver properties <I>in vitro</I>. We encapsulated HepaRG cells, either alone or with supporting cells, in a biodegradable hybrid hydrogel. The elastic modulus of the 3D liver dynamically changed during culture due to the combined effects of prolonged degradation of hydrogel and extracellular matrix formation provided by the supporting cells. As a result, when the elastic modulus of the 3D liver model converges close to that of the <I>in vivo</I> liver (≅ 2.3 to 5.9 kPa), both phenotypic and functional maturation of the 3D liver were realized, while hepatic gene expression, albumin secretion, cytochrome p450-3A4 activity, and drug metabolism were enhanced. Finally, the 3D liver model was expanded to applications with embryonic stem cell-derived hepatocytes and primary human hepatocytes, and it supported prolonged hepatocyte survival and functionality in long-term culture. Our model represents critical progress in developing a biomimetic liver system to simulate liver tissue remodeling, and provides a versatile platform in drug development and disease modeling, ranging from physiology to pathology.</P> <P><B>Statement of Significance</B></P> <P>We provide a functionally improved 3D liver model that recapitulates <I>in vivo</I> liver stiffness. We have experimentally addressed the issues of orchestrated effects of mechanical compliance, controlled matrix formation by stromal cells in conjunction with hepatic differentiation, and functional maturation of hepatocytes in a dynamic 3D microenvironment. Our model represents critical progress in developing a biomimetic liver system to simulate liver tissue remodeling, and provides a versatile platform in drug development and disease modeling, ranging from physiology to pathology. Additionally, recent advances in the stem-cell technologies have made the development of 3D organoid possible, and thus, our study also provides further contribution to the development of physiologically relevant stem-cell-based 3D tissues that provide an elasticity-based predefined biomimetic 3D microenvironment.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>