VP-16으로 성공적으로 치료된 위 Kaposi 육종 1예

양웅석,송근암,조몽,이재승,송철수,김형욱,김영민,이수봉,김태오,이성훈 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.4

The skin is an uncommon site of distant metastasis from any internal malignancy, and the incidence of metastatic skin lesions as the first symptom of disease is only 0.8% in patients with all systemic malignancies. Furthermore, cutaneous metastasis from adenocarcinoma of the stomach has been found to be extremely rare. A 35-year-old female patient was admitted due to multiple cutaneous nodules in her chest, abdomen, and back. A gastroendoscopic examination and biopsy was made according to the results of skin pathologic findings. Stomach and skin biopsy results revealed a signet ring cell type of adenocarcinoma. A case of gastric adenocarcinoma in which metastatic skin nodules appeared as the first sign of disease, is here in reported with a review of related literature.

人體重心과 體格 및 體力에 關한 硏究(Ⅰ) : 國民學生 5 ·6學年을 中心으로

李永雄,朴秀一 慶尙大學校 1980 論文集 Vol.19 No.1

1. The purpose of this study is to investigate the height of the center of gravity of a body according to physique, and is also to investigate the difference of physical fitness by the physique according to the height of the center of gravity of a body. 2. Physique was measured by the height and weight of the subjects and classified into five ranks of physical progressions, and then the subjects were tested in five items; 100m sprint, standing broad jump, flexed arm-hamging(female) and pull-ups(male), ball throwing and sit-up. Next, the position of the center of gravity of a body was measured by "the method fo Reynolds-Lovett". 3. In the height of the center of gravity of a body, the ranks of the height were as follows; A class was the lst, B class was the 2nd, C class was the 3rd, D class was the 4th, and E class was the 5th. 4. The average height of the center of gravity of a body by age and sex might be summarized as follows. The center of 10 year-old girls was located in 56.21% point of the standing height. That of 11 year-old girls was located in 57.51/%point. That of 10 year-old boys was located in 57.24% point. That of 11 year-old boys was located in 57.93% point. Therefore, the center of gravity of a body in case of girls was situated in almost the same height as in case of boys and was higher than in case of grown-up women. 5. In physical fitness, E class was a little inferior to the other classes, but any difference among the other classes was not found out.

장내세균에 의한 Trichothecene Mycotoxins의 대사 : (I) T-2 toxin의 대사

이웅수 忠州大學校 2008 한국교통대학교 논문집 Vol.43 No.-

Trichothecene mycotoxins are a chemically related group of toxic fungal metabolites produced by a number of species of the fungi such as Fusarium, Cephalosporium, Trichothecium, Myrothecium, Stachybotrys and Trichoderma, and are responsible for mycotoxicoses as causative agents in a wide variety of animal and human health problems. Especially, T-2 toxin is a secondary metabolite produced by Fusarium spp. such as F. tricinctum, F. poae and F. sporotrichioides etc., and is often found in agricultural products and feeds including cereals, and is a potent cytotoxic and immunodepressive trichothecene mycotoxin, and causative agent of moldy corn toxicosis and alimentary toxic aleukia(ATA), and induces acute toxicity such as leukocytosis, hemorrhage and dermal edema, followed by death in animals. In order to elucidate the possible metabolism of T-2 toxin by rat and human intestinal bacteria, this research was carried out. T-2 toxin transformed into HT-2 toxin and unknown metabolite by cultured intestinal bacteria in the anaerobic condition. The amount of HT-2 toxin was decreased, but unknown metabolite was increased by subsequent incubation. This metabolite was elucidated to deepoxy HT-2 toxin by NMR and mass spectra data. It was suggested that T-2 toxin was deepoxidized after deacetylation to HT-2 toxin by the intestinal bacteria. This deepoxy HT-2 toxin was transformed into HT-2 toxin and 15-deacetyl-deepoxy HT-2 toxin by liver microsome of PCB-treated rat in the presence of NADPH and oxygen. These results suggest that the epoxide group of trichothecene mycotoxins is reduced by intestinal bacteria and re-oxidized by hepatic microsomal enzyme of rat.

集團給食所 및 家庭의 廚房에서 使用하는 행주의 衛生學的 處理에 관한 硏究

李雄洙,崔錫南 충주대 2003 産業科學論文集 Vol.11 No.-

This study was carried out to find out the method on hygienic treatment of dishcloth used at the cookroom of mass meal service center and household in order to prevent food poisoning and oral infectious disease. This research was examined with three methods(washing, boiling and chlorine solution treatment) based on materials for the questionnaire by 28 persons who engaged in mass meal service center and telephone interview by 100 housewives. The indicator bacteria on the fecal contamination in food hygiene such as Escherichia coli, Staphylococcus aureus, Brochothrix thermosphacta, Salmonella typhimurium, vibrio parahae-molyticus and pseudomonas aeruginosa, and Shigella sonnei, one of oral epidemic bacteria, were used in this study. The results of this study related to food hygienic and micro-biological tests were the following : 1) By washing treatment, the indicator bacteria and Shigella sonnei were detected less 1/2 to 1/4 than initial viable cell counts. 2) After boiling treatment for 10min, E. coli, Pseudo. aeruginosa and Brocho. thermo-sphacta were found 63-340 CFU/㎖, and Staphy. aureus and Sal. typhimurium were detected 1-2CFU/℃, but Vibrio parahaemolyticus and Shigella sonnei not detected. 3) After boiling treatment for 20min, E. coli, Brocho. thermosphacta and Pseudo. aeru-ginosa were detected 4-130CFU/℃, but Staphy. aureus, Sal. typhimurium, Vibrio par-ahaemolyticus and Shigella sonnei died out completely. 4) After boiling treatment for 30min, only Pseudo. aeruginosa was found, and the other bacteria were not detected. 5) In case of soaking treatment in chlorine solution, all tested bacteria were detected to 1-2500 CFU/℃ in 80ppm solution. In 160ppm chlorine solution, Brocho. thermosphacta, Sal. typhimurium, Vibrio parahaemolyticus, Pseudo. aeruginosa and Shigella sonnei were existed to 2-32CFU/℃, but E. coli and Staphy. aureus were died out. In 320ppm chlorine solution, only Pseudo. aeruginosa was found to 13CFU/℃, and the other bacteria were not detected. In 640ppm chlorine solution, all tested bacteria were not detected. In conclusion, this study suggested that boiling over 30min and soaking in 640ppm chlorine solution are very good methods as the hygienic treatment of dishcloth contaminated with food poisoning and oral infectious disease bacteria.

설사환자의 대변에서 분리한 세균성 병원체의 특성 조사 II. Staphylococcus aureus의 분리·동정 및 항생제에 대한 내성 조사

이웅수 忠州大學校 2010 한국교통대학교 논문집 Vol.45 No.-

I investigated the characterization of bacterial pathogens on 2,270 stool specimens, which was collected from dysentery patients for one year, 2007 Detection of enteric bacteria in stool of diarrheal patient was performed according to the procedure of routine protocol such as culture and multiplex PCR method. Biochemical test for the identification of Staphylococcus aureus(S. aureus) was performed by API Staphy kit. In order to cofirm of toxin type, I also carried out serological test. Among 456 isolates of bacterial pathogen isolated from the collected stool, S. aureus was 74 isolates(toxin A type 16, C type 9, D type 1, non-toxin type 48). Detection rate of S. aureus in dysentery patients was the higher in April, August and December, and in children below 10 years old(66.2%). In addition, S. aureus in male was isolated higher rate than their in female. Antibiotics resistance rate on the each toxin type S. aureus showed that type A is AM > PC > EM, OC > TC > CP > CT > CM > GM, IP > CI > ChP > TM/SM, RP, and type C is AM, PC, EM, OC > CP, CM, TC > CT, CI, IP > GM > TM/SM, RP, and type D is only 100% on AM, PC and TC. In the present study, I focused to investigate the incidence and antibiotics resistance patterns in each toxin type S. aureus related diarrheal patients. These results were very helpful to make preventive method for infectious disease causing bacterial gastroenteritis, especially, food poisoning by enteric bacterial infection.

강박증 환자에서의 인격장애 공존병리에 관한 연구

이동우,강웅구,류인균,정도언,조맹제,우종인,김용식,권준수 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.3

연구목적 : 강박증의 동반인격장애에 관한 연구들은 기존의 개념과 달리 강박성 인격장애와 강박증 간에는 특이적인 관계가 없으며, 강박증 환자들은 다양한 인격장애를 동반한다는 결과를 보여주었다. 그러나 이러한 연구들은 동반 인격장애에 관한 기술적인 분석에 그쳤고, 강박증 환자군의 임상 증상과 동반 인격장애 양상간의 연관성에 관한 분석을 시행하지 않았다. 본 연구의 목적은 강박증 환자군에서의 동반 인격장애를 평가하고 강박증 환자의 다양한 증상과 동반 인격특성 간의 연관성에 대해 연구하는 것이다. 방 법 : DSM-IV의 강박증의 진단기준을 만족하는 59명의 환자군과 32명의 정상대조군을 대상으로 인격장애 질문지를 적용한 후 4∼6점의 절단점을 기준으로 인격장애의 진단이 내려졌다. 먼저 환자군과 정상대조군 간의 특정 인격장애 빈도의 차이를 알아보기 위하여 Fisher's exact test를 시행한 후, 두집단 간에 유의한 차이가 있는 것으로 밝혀진 인격장애 척도 점수에 미치는 Y-BOCS,BDI,BAI점수의 영향을 알아보기 위하여 다중선형회귀 분석을 시행하였다. 결 과 : 1) Fisher's exact test 결과 강박증 환자군은 정상 대조군에 비해 경계성 인격장애(18.6%vs 0%, p=0.007). 회피성 인격장애(55.9% vs 18.8%, p=0.001), 우울성 인격장애(40.7% vs 0%, p=0.000)의 비율이 유의하게 높았다. 2) 다중선형회귀분석 결과 Y-BOCS 점수 (t=2.10, p=0.043)와 BAI점수(t=2.60, p=0.014)는 경계성 인격장애 점수에 유의한 영향을 주는 것으로 나타났다. 또한 BAI점수(t=3.23, p<0.003)는 회피성 인격장애 저수에, BDI점수 (t=3.08, p=0.004)는 우울성 인격장애 점수에 유의한 영향을 주는 것으로 나타났다. 결 론 : 강박증 환자군은 정상 대조군에 비해 회피성 인격장애, 우울성 인격장애, 경계성 인격장애의 동반율이 높고, 강박증이 경계성 인격장애의 심각도에 유의한 영향을 미치는 것으로 사료된다. The purpose of this study was to assess the comorbid axis-Ⅱ disorders of obsessive compulsive disorder(OCD) patients and to investigate the relationship between symptoms of OCD and the comorbid personality traits. The subjects were 59 patients who met DSM-IV criteria for obsessive-compulsive disorder and 32 normal controls. All subjects completed personality Disorder Questionnaire-IV(PDQ-IV). The patients completed Beck Depression Inventory(BDI) and Beck Anxiety Inventory(BAI), and were rated with Yale-Brown Obsessive Compulsive Scale(YBOCS). The results were as follows. 1) The OCD patients showed significantly higher prevalence of avoidant, depresssive and borderline personality disorder(p<0.01) compared to controls. 2) The BAI score had significant effect on the avoidant personality score(t=3,23, p<0.003). The BDI score had significant effect on the depressive personality score(t=3.08, p=0.004). The YBOCS(t=2.10, p=0.043) and BAI(t=2.60, p=0.014) scores had significant effects on the borderline personality score. We found that OCD patients had higher prevalence of avoidant, depressive, and borderline personality disorders. We also found that obsessive-compulsive symptoms have significant effect on the severity of borderline personalty traits. We suggest that it would be very helpful to consider Axis-Ⅱ disorders for managing patients with obsessive-compulsive disorder.

수입 중국산 개나리 열매(연교)의 소염효과에 관한 연구

이응식,조재민,민윤식 충주대 산업과학기술연구소 1996 産業科學論文集 Vol.4 No.-

In order to determine whether methanol extract of Chinese Forsythiae fructus inhibite acute and chronic inflammatory responses induced with histamine and carrageenin in rat, this research was carried out. Inhibitory effect on the pigment leakage induced with histamine 50㎍ by methanol extract 0.15g per 150g body weight was shown positive result significantly. In the experiment by carrageenin paw edema method, maximum inhibitory effects were shown 1hr and 30min after orally administrated with 0.03g and 0.3g methanol extract per 150g body weight, respectively. In the inhibitory effect by methanol extract on the acute inflammation induced by granuloma pouch method, it was shown that inflammatory exudate of granuloma pouch and wet weight of granulation tissue reduced. In the chronic inflammation by granuloma pouch method, inhibitory effect was observed but significant inhibition was not observed statistically. The results of this research were shown that methanol extract of Chinese Forsythiae fructus has inhibitory effects on the acute and chronic inflammation, and were similar to those reported on Korean Forsythiae fructus.

Aflatoxin B₁의 간발암성에 대한 nivalenol의 영향

李雄洙 충주대 산업과학기술연구소 1999 産業科學論文集 Vol.7 No.2

This research was carried out to investigate the toxicological effect of long-term intake of nivalenol(NIV), a trichothecene mycotoxin produced by Fusarium nivale Fn 2B, and the combined toxicity of NIV and aflatoxin Bl(Af-Bl), a potent hepatocarcinogenic mycotoxin, in mice. In male mice, liver tumors developed to all Af-Bl treated groups, and most of them were hepatocellular carcinomas. The incidence of Af-Bl induced liver tumors was not altered by feeding of NIV containing diet. In female mice, however, the incidences of liver tumors were 31%, 20% and 0% in Af-Bl alone group, Af-Bl plus NIV 6ppm group and Af-Bl plus NIV 12ppm group, respectively. NIV dose dependent pattern on hepatocarcinogenesis by Af-Bl showed a decreasing tendency. Incidence of liver tumors in NIV 12ppm group was significantly lower than in Af-Bl alone group. Most of liver tumors in female mice were hepatocellular adenomas, and the malignancy of Af-Bl induced liver tumors was lower than that of male mice. The remarkable change of biochemical factor on serum was not observed by administration of NIV. In these results, NIV has no tumorous effect in mice, and increased significantly life span. And the inhibition effect of NIV on Af-Bl induced hepatocarcinogenesis was suggested in mice.

Rat의 간세포 항산화계 및 지질 과산화에 미치는 Mycotoxins의 영향 : I. Effects of Fumonisin B₁, a Mycotoxin produced by Fusarium moniliforme I. Fumonisin B₁의 영향

李雄洙 忠州大學校 1994 한국교통대학교 논문집 Vol.29 No.2

The purpose of this study was to determine if fumonisin B₁(FB₁), a promoter for hepa tocarcinogenesis in rat, induces lipid peroxidation and alters the concentrations of antioxidant systems in rat liver cell. The hepatocytes were isolated from male Harlan Sprague-Dawley rat by using aseptic procedures, and treated with varying concentrations of FB₁(1μM,10μM and 50μM). The levels of malondialdehyde(MDA), conjugated diene(CD), vitamin A(V-A), vitamin E(V-E),γ-tocopheryl quinone(γ-TQ), vitaminC(V-C), glutathione(GSH), total lipids(TL), and protein were determined to assess oxidative damage and antioxidant status on the hepatic cell. FB₁ increased the levels of MDA and CD, oxidative products of cellular lipid. And FB₁ decreased the levels of cellular antioxdants such as V-E, and V-A, and V-C, but increased GSH level. This study shows that the adiministration of FB₁, a mycotoxin produced by Fusarium moniliforme, did increase indices of lipid peroxidation, but that cellular antioxidant defences were inhibited by FB₁.

Anthraquinone계 화합물의 변이원성 및 유전독성 발현에 관한 연구(Ⅱ) : On the strand scission ability of plasmid DNA chain by emodin Emodin의 plasmid DNA chain 절단능에 관한 연구

李雄洙 忠州大學校 1999 한국교통대학교 논문집 Vol.34 No.2

It has been reported that several anthraquinone compounds possess mutagenicity and carcinogenicity. Emodin(1, 3, 8-trihydroxy-6-methylanthraquinone)is one of the anthraquinone mycotoxins, and a constituent of several herb medince used as laxative such as Rhei Rhizoma(rhubarb), Cassiae Semen(cassia seed) and Aloe. Emodin was selected as a model compound to solve a carcinogenic mechanism of the anthraquinone mycotoxins and to evaluate the safety of herb medicine. This research was carried out on the genotoxic effects of emodin activated by cytochrome P-450 and on the strand scission ability of plasmid DNA chain by emodin. To investigate the correlation between mutagenicity and DNA strand scission, plasmid DNA pUC19 was treated with emodin and 2-OH-emodin under the various conditionas, and followed the analysis of strand breaks by agarose gel electrophoresis. When emodin and 2-OH-emodin incubated for 1hr at 37℃ , the form Ⅱ(nicked) DNA was slightly increased. In 30min at 65℃, that was remarkably increased. In the presence of reducing agents such as NaBH₄and dithiothreitol(DDT), the formation of the form Ⅱ was slightly accelerated by 2-OH-emodin, and was remakably increased by emodin. Form these results, it was suggested that active oxygens have been generated by the reduction-oxidation cycle such as guinone ?? hydroquinone. The formation of formⅡ induced with emodin decreased by catalase ae H₂O₂quencher, and DMSO and ethanol as hydroxyl radical scavenger in the presence of NaBH₄. To clarify the site of DNA strand sicssion, the nick translation method was introduced, and the radioactivity of[α-³²P] dCMP incorporated into 3'OH terminal of the double strand DNA was counted. The incorporation of [α-³²P] dCMP into emodin-and 2-OH-emodin-treated DNA decreased to 60% and 73% of the controls, respectively. In the presence of NaBH₄, the incorporations were almost same as the control. From these results, it was presumed that the nick site possesses not 3'OH terminal but 3'P. The incorporated radioactivity was increased by treatment of alkaline phospatase in order to dephosphorylate the terminal phosphate residue. It was suggested that active oxygens generated from emodin caused DNA strand scission, and the nick site was presumed to 3'P terminal, not 3'OH. In conclusion, DNA damages by emodin were caused by the fllowing two pathways, i.e emodin is biotransformed into 2-OH-emodin, a direct-acting mutagen by cytochrome P-450, and activated emodin and hydroxyl radical by the reductase. Such approaches are expected to solve the basic mechanism of emodin-induced genotoxicity.