http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : ( Sunhyae Jang ) (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
Role of Arachidonic Acid in Promoting Hair Growth
( Semchin Munkhbayar ),( Sunhyae Jang ),( A Ri Cho ),( Soon Jin Choi ),( Chang Yup Shin ),( Hee Chul Eun ),( Kyu Han Kim ),( Ohsang Kwon ) 대한피부과학회 2016 Annals of Dermatology Vol.28 No.1
Background: Arachidonic acid (AA) is an omega-6 polyunsaturated fatty acid present in all mammalian cell membranes, and involved in the regulation of many cellular processes, including cell survival, angiogenesis, and mitogenesis. The dermal papilla, composed of specialized fibroblasts located in the bulb of the hair follicle, contributes to the control of hair growth and the hair cycle. Objective: This study investigated the effect of AA on hair growth by using in vivo and in vitro models. Methods: The effect of AA on human dermal papilla cells (hDPCs) and hair shaft elongation was evaluated by MTT assay and hair follicle organ culture, respectively. The expression of various growth and survival factors in hDPCs were investigated by western blot or immunohistochemistry. The ability of AA to induce and prolong anagen phase in C57BL/6 mice was analyzed. Results:AA was found to enhance the viability of hDPCs and promote the expression of several factors responsible for hair growth, including fibroblast growth factor-7 (FGF-7) and FGF-10. Western blotting identified the role of AA in the phosphorylation of various transcription factors (ERK, CREB, and AKT) and increased expression of Bcl-2 in hDPCs. In addition, AA significantly promoted hair shaft elongation, with increased proliferation of matrix keratinocytes, during ex vivo hair follicle culture. It was also found to promote hair growth by induction and prolongation of anagen phase in telogen-stage C57BL/6 mice. Conclusion: This study concludes that AA plays a role in promoting hair growth by increasing the expression of growth factors in hDPCs and enhancing follicle proliferation and survival.
-
Lee, Woong-Hee,Jang, Sunhyae,Lee, Jung-Suk,Lee, Young,Seo, Eun-Young,You, Kwan-Hee,Lee, Seung-Chul,Nam, Kwang-Il,Kim, Jin-Man,Kee, Sun-Ho,Yang, Jun-Mo,Seo, Young- Joon,Park, Jang-Kyu,Kim, Chang Deok,L The Society for Investigative Dermatology, Inc 2005 The Journal of investigative dermatology Vol.125 No.5
We isolated a human gene encoding keratinocyte proline-rich protein (hKPRP). hKPRP gene is located in the region of epidermal differentiation complex on chromosome 1q21, and its ∼2.5 kb mRNA encodes 579 amino acid protein with high proline content (18%). The mRNA level of hKPRP was markedly increased at both 7 and 14 d after treatment with 1.2 mM calcium in cultured normal human epidermal keratinocytes. In situ hybridization demonstrated that hKPRP was expressed in upper granular layer of normal epidermis with characteristic intermittent pattern. In psoriatic lesion, hKPRP expression was increased as compared with normal skin and showed continuous pattern. Immunohistochemical analysis also confirmed the expression of hKPRP at the protein level. Western blot analysis showed that hKPRP protein of ∼70 kDa size was significantly increased by calcium in a time-dependent manner. In mouse tissue blot assays, the expression of KPRP was detected in stomach and skin tissues, and began at 17.5 embryonic days. Additionally, hKPRP expression was detected in the periderm of human fetal skin from 16 wk estimated gestational age. Together, these results suggest that hKPRP is an epidermal marker expressed in stratified squamous epithelia and has a potential role in keratinocytes differentiation.Journal of Investigative Dermatology (2005) 125, 995–1000; doi:10.1111/j.0022-202X.2005.23887.x
-
( Ji Su Lee ),( Youngae Lee ),( Sunhyae Jang ),( Dong Hun Lee ),( Soyun Cho ) 한국피부장벽학회 2022 한국피부장벽학회지 Vol.24 No.2
Background: Epidemiological studies have demonstrated that particulate matter (PM) exposure can cause the development and exacerbation of atopic dermatitis (AD). However, the mechanisms of how PM affects AD are still unclear. Recently, pregnane X receptor (PXR), one of xenobiotic receptors, has been reported to be related to skin inflammation in AD. Objective: This study aimed to investigate PM-induced effects on AD and the role of PXR in vivo and in vitro. Methods: Standard reference material of PM (SRM 2786) was treated in mouse and human keratinocyte (HaCaT) with AD-like inflammation, which was made by treating 2, 4-dinitrochlorobenzene (DNCB) in BALB/C mice and treating TNF-α and IFN-γ in HaCaTs, respectively. The role of PXR was investigated with PXR siRNA-transfected and rifampicin (a potent activator of human PXR) treated HaCaTs. Results: PM significantly increased dermatitis score and skin thickness in mice with AD-like inflammation. After PM application, mRNA and protein levels of Th17-related cytokines and chemokines (IL-1β, IL-6, IL-17A, IL-23A, and CCL20) increased both in mice and HaCaTs with AD-like inflammation. In parallel, PXR was activated after PM application, and PXR activation repressed NF-κB expression. Of note, the PM-induced Th17 inflammation was exaggerated in PXR siRNA-transfected HaCaTs. In contrast, Th17 inflammation was decreased after rifampicin treatment. Conclusions: PM exposure induces Th17 inflammation and PXR activation in AD. PXR seems to have a protective role in the PM-induced Th17 inflammation in AD by being activated by PM and suppressing NF-κ B. These results suggest that PM triggers AD progression to chronic disease and PXR could be a treatment target to attenuate the progression to chronic disease.
-
Enhancement of Human Hair Growth Using Ecklonia cava Polyphenols
( Hyoseung Shin ),( A Ri Cho ),( Dong Young Kim ),( Semchin Munkhbayer ),( Soon Jin Choi ),( Sunhyae Jang ),( Seong Ho Kim ),( Hyeon Cheol Shin ),( Oh Sang Kwon ) 대한피부과학회 2016 Annals of Dermatology Vol.28 No.1
Background: Ecklonia cava is a brown alga that contains various compounds, including carotenoids, fucoidans, and phlorotannins. E. cava polyphenols (ECPs) are known to increase fibroblast survival. The human dermal papilla cell (hDPC) has the properties of mesenchymal-origin fibroblasts. Objective: This study aims to investigate the effect of ECPs on human hair growth promotion in vitro and ex vivo. Methods:MTT assays were conducted to examine the effect of ECPs on hDPC proliferation. Hair growth was measured using ex-vivo hair follicle cultures. Real-time polymerase chain reaction was performed to evaluate the mRNA expression of various growth factors in ECP-treated hDPCs. Results: Treatment with 10 μg/ml purified polyphenols from E. cava (PPE) enhanced the proliferation of hDPCs 30. 3% more than in the negative control (p<0. 001). Furthermore, 0. 1 μg/ml PPE extended the human hair shaft 30. 8% longer than the negative control over 9 days (p<0. 05). Insulin-like growth factor-1 (IGF-1) mRNA expression increased 3. 2-fold in hDPCs following treatment with 6 μg/ml PPE (p<0. 05). Vascular endothelial growth factor (VEGF) mRNA expression was also increased 2. 0-fold by 3 μg/ml PPE (p<0. 05). Treatment with 10 μg/ml PPE reduced oxidative stress in hDPCs (p<0. 05). Conclusion: These results suggest that PPE could enhance human hair growth. This can be explained by hDPC proliferation coupled with increases in growth factors such as IGF-1 and VEGF. Reducing oxidative stress is also thought to help increase hDPCs. These favorable results suggest that PPE is a promising therapeutic candidate for hair loss.
ScienceON
KISS연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
