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DNA barcoding of the stem borer, Stathmopoda spp. (Lepidoptera: Stathmopodidae)
Sora Kim,Wonhoon Lee,Yerim Lee,Seunghwan Lee 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10
The mitochondrial COI gene has proven successful for identifying the lepidopteran species. In addition, this gene has been applied to recognize cryptic species and confirm a polyphagous species as one species. However, it has been also reported misidentification in the COI gene. As a result, some researchers have suggested the use of additional genes in species identification, especially, nuclear genes. In this study, we observed that a nuclear EF1agene is better than the mitochondrial COI gene in recognizing Stathmopoda species. For example, among the all species, 47 individuals of nine species, a large intraspecific pairwise difference were detected, up to 15.0% in universal barcoding regions of the COI gene, 22.0% in other part of the COI gene; however, 2.0% in EF1a. In contrast, Stathmopoda sp2 and S. commoda were separated into several clusters in each different COI regions, and some individuals of S. auriferella and S. commoda, were closer to each other separating from the cluster of the same species. But, we get clear results from the EF1a, Stathmopoda species well clustered and could be distinguished.
Lepidopteran Insect Pests on Grape, Vitis vinifera L., from Korea
Sora Kim,Wonhoon Lee,Sun-Kook Kim,Seunghwan Lee 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.05
Until now, seven species, Acosmeryx nega, Herpetogramma luctuosalis, Ostrinia furnacalis, Endoclyta excrescens, Spodoptera litura, Paranthrene regalis, and Nippoptilia vitis, have been known as lepidopteran grape pests in Korea (Woo, 1980; Lee, 1981; Kim, 1991; NIAST, 2002; Korea grape community, 2009). We discovered thirteen additional species belonging to eight families from Gwangwon and Chungbuk provinces, Korea. Additionally, we corrected the previous records of Deuterocopus albipunctatus and Nppoptilia vitis. They were identified by adult external or genitalic characters first, and also confirmed by the COI marker, compared with two public databases, NCBI and BOLD.
Lee, Choong-kun,Kim, Sora,Lee, Jae Seok,Lee, Jeong Eun,Kim, Sung-moo,Yang, In Seok,Kim, Hye Ryun,Lee, Jeong Ho,Kim, Sangwoo,Cho, Byoung Chul Elsevier 2017 Lung cancer Vol.113 No.-
<P><B>Abstract</B></P> <P><B>Objectives</B></P> <P>Despite initial responses to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) in EGFR mutant non-small cell lung cancer, patients invariably develop acquired resistance. In this study, we performed next-generation sequencing in pre- and post-EGFR-TKI tumor samples to identify novel resistance mechanisms to EGFR-TKIs.</P> <P><B>Material and methods</B></P> <P>We collected tumor tissues before EGFR-TKI treatment and after progression from 19 NSCLC patients to analyze genomic alterations in 409 cancer related genes. Bioinformatics analyses were used to identify mutations in which the allele frequencies are significantly changed, or newly appeared after progression.</P> <P><B>Results</B></P> <P>Overall, mutation rates and compositions were similar between pre- and post-EGFR-TKI tumors. We identified EGFR T790M as the most common mechanism of acquired resistance (63.2%). No pre-EGFR-TKI tumor had a preexisting T790M mutation, suggesting that tumors acquired T790M mutations following progression on EGFR-TKIs. Compared to T790M-positive tumors, T790M-negative tumors showed relatively high tumor mutation burden and shorter survival, suggesting T790M-negative patients as a potential candidate for immune checkpoint inhibitors. TP53 mutation was also significantly enriched in the T790M-negative tumors. Finally, we described here for the first time a novel missense mutation (T263P), which occurred concurrently with an activating G719A mutation, in the extracellular domain II of EGFR in a patient with poor response to erlotinib. Ba/F3 cells harboring EGFR T263P/G719A mutation showed higher sensitivity to afatinib, compared to gefitinib due to inhibition of EGFR/HER2 heterodimerization.</P> <P><B>Conclusion</B></P> <P>Comprehensive genomic analysis of post-EGFR-TKI tumors can provide novel insight into the complex molecular mechanisms of acquired resistance to EGFR-TKIs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> NGS in paired NSCLC before and after EGFR-TKIs was performed. </LI> <LI> EGFR-TKI resistant T790M (+) NSCLC patients had significantly longer survival. </LI> <LI> Hypermutation/TP53 mutation seem enriched in EGFR-TKI resistant T790M (−) patients. </LI> <LI> Tumor mutation burden seem to be higher in EGFR-TKI resistant T790M (−) patients. </LI> <LI> T263P of EGFR was identified as the de novo mechanism of resistance to EGFR-TKIs. </LI> </UL> </P>
Evaluation of Circulating MicroRNA Biomarkers in the Acute Pancreatic Injury Dog Model
Lee, Han-Byul,Park, Hyun-Kyu,Choi, Hyun-Ji,Lee, Sora,Lee, Sang-Joon,Lee, Ji-Young,Cho, Eun-Ho,Han, Hyo-Jeong,Seok, Ju-Hyung,Son, Woo-Chan MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.10
<P>This study aimed to evaluate the usefulness of four microRNAs (miRNAs) in an acute pancreatic injury dog model. Acute pancreatitis was induced by infusion of cerulein for 2 h (7.5 μg/kg/h). The levels of well-known miRNAs, microRNA-216a (miR-216a) and microRNA-375 (miR-375), and new candidates microRNA-551b (miR-551b), and microRNA-7 (miR-7), were measured at 0, 0.5, 1, 2, 6, 12, and 24 h with serum amylase and lipase, and histopathological examination was performed. Among the four miRNAs, miR-216a and miR-375, and serum enzymes were significantly increased by cerulein treatment. The expression levels of miRNAs and serum enzymes peaked at 2–6 h with a similar pattern; however, the overall increases in miR-216a and miR-375 levels were much higher than those of the serum enzyme biomarkers. Increased levels of miR-216a and miR-375 were most highly correlated to the degree of individual histopathological injuries of the pancreas, and showed much greater dynamic response than serum enzyme biomarkers. Twenty-four-hour time-course analysis in this study revealed time-dependent changes of miRNA expression levels, from initial increase to decrease by predose level in acute pancreatitis. Our findings demonstrate that, in dogs, miR-216a and miR-375 have the potential to sensitively detect pancreatitis and reflect well the degree of pancreatic injury, whereas miR-551b and miR-7 do not.</P>
Family Pterophoridae (Lepidoptera: Pterophoroidea) in Korea
Sora Kim,Kyu-Tek Park,Bong-Kyu Byun,Bong-Woo Lee,Hyojoong Kim,Sunghoon Jung,Wonhoon Lee,Seunghwan Lee 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10
The family Pterophoridae (Lepidoptera), plume moths, consist of 1,136 species listed in the ‘world catalogue’ by Gielis (2003), and a few more described since then. Approximately 250 species are known in the Palaearctic Regions (Arenberger, 1995). In this study, 35 species of 21 genera have been reviewed from Korea (Matsumura 1931, 1938; Zoological Society of Korea 1968; Park 1983; Arenberger 1991; Kim et al., 2010a, 2010b, 2012a, 2012b). We suggest the problematic species, Platyptilia ignifera, is morphologically and genetically separated from congeneric species supporting the possibility as a new genus, and employ the classification system of Arenberger (1995) rather than that of Gielis (1993) using parsimony based on morphological characters.
Sora Kim,Wonhoon Lee,Sunghoon Jung,Bong-Kyu Byun,Bong-Woo Lee,Seunghwan Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10
Systematic studies within the family Pterophoridae have been determined by external morpholgical characteristics (Yano 1963, Spuler 1910, Meyrick 1910). However, it is sometimes to be the result of species misplacement by the variation, depending on geographic isolation and genetic drift (Moran, 1986; Shufran et al., 2000; Anstead et al., 2002; Margaritopoulos et al., 2006). The genus Platyptilia Hübner [1925] is one of the largest genera of the family comprising more than 100 species according to the World’s Catalog by Gielis (2003) mainly distributed from Palaearctic and Afrotropical regions. Within the genus, Platyptilia ignifera has been suspected as very differentiated from other congeneric species in the morphological characters. In this study, to ascertain a placement of the species, we performed molecular analysis with one mitochondrial gene, COI and one nuclear gene, 28S, and morphometric analysis based on six ratios characters using principal components analysis (PCA). The sequences of the two genes, COI, 28S, implied that Platyptilia ignifera separated from Platyptilia spp. In addition, P. ignifera exhibited morphological characteristics distinct from other congeneric species. Based on these results, we propose that Neoplatyptilia gen. nov., for Platyptilia ignifera.
A thermally and mechanically stable solar cell made of a small-molecule donor and a polymer acceptor
Oh, Sora,Badgujar, Sachin,Kim, Da Hun,Lee, Wang-Eun,Khan, Nasir,Jahandar, Muhammad,Rasool, Shafket,Song, Chang Eun,Lee, Hang Ken,Shin, Won Suk,Lee, Jong-Cheol,Moon, Sang-Jin,Lee, Sang Kyu The Royal Society of Chemistry 2017 Journal of materials chemistry. A, Materials for e Vol.5 No.30
<▼1><P>Highly efficient and stable BDT2TR:PNDI-2T organic solar cells are investigated. Although this system shows a PCE of 4.43%, significant enhancements are observed in the thermal stability, high thickness tolerance, and flexibility as compared with the PC71BM-based organic solar cells.</P></▼1><▼2><P>We performed systematic experiments based on a small molecule donor and a polymer acceptor containing a naphthalene diimide (NDI)-based polymer as compared with fullerene-based acceptor (PC71BM) solar cells. Among polymer acceptors, the NDI-based polymer (PNDI-2T) shows good properties such as broad light absorbance with a strong absorption co-efficient and a well oriented crystalline structure leading to high electron mobility. We monitored the photovoltaic properties of both PNDI-2T and PC71BM acceptors with a BDT2TR donor. Although the BDT2TR:PC71BM device showed a higher PCE of 8.20%, the BDT2TR:PNDI-2T device also showed remarkable photovoltaic results with a PCE of 4.43%, <I>V</I>OC of 0.86 V, <I>J</I>SC of 7.26 mA cm<SUP>−2</SUP>, and FF of 71% indicating one of the highest efficiencies for small molecule donor and non-fullerene polymer acceptor systems. In particular, the PNDI-2T acceptor showed excellent thermal stability and intrinsic mechanical performance as compared with the PC71BM acceptor. To demonstrate the potential of the polymer acceptor for solar devices, we fabricated a device for testing thermal stability, high thickness tolerance, and the flexibility of the solar cell with bending stress. As a result, the PNDI-2T-based solar cell exhibited excellent thermal stability at 150 °C for 15 h and the PCE of the BDT2TR:PNDI-2T device with a thick active layer (around 610 nm) maintained 80% of its initial value. Moreover, the flexible device with the BDT2TR:PNDI-2T system retained its homogeneous morphology and showed maintained photovoltaic performance even after 100 bending cycles. Therefore, PNDI-2T based organic solar cells have good potential for application as flexible and portable real energy generators.</P></▼2>