Cloning and DNA Sequence of Carboxymethylcellulase(CMCase) Gene from Cellulomonas sp. YE-5

HER, SONG,KIM, DONG SEOB,CHOI, SUN JIN,OH, DOO HWAN 한국미생물 · 생명공학회 1993 Journal of microbiology and biotechnology Vol.3 No.2

CMCase positive clones were screened from Cellulomonas sp. YE-5 and named pCE1, pCE2 and pCE3. Among the positive clones pCE1 was used for this study, because it has the smallest insert and the highest CMCase activity among the 3 clones, and its nucleotide sequence was determined. The CMCase gene in pCE1 was composed of 1071 by of nucleotides coding 357 amino acids. Computer analysis showed that the pCE1 has 65% sequence homology with the endoglucanase from Cellulomonas fimi.

Trichostatin A stimulates steroid 5alpha-reductase gene expression in rat C6 glioma cells via a mechanism involving Sp1 and Sp3 transcription factors.

Her, Song,Lee, Mi-Sook,Morita, Kyoji Birkhäuser Boston 2010 Journal of molecular neuroscience Vol.41 No.2

<P>The adrenergic and serotonergic stimulations of rat C6 glioma cells have previously been shown to induce the activation of steroid 5alpha-reductase (5alpha-R) gene expression, resulting in their differentiation through the production of neuroactive 5alpha-reduced steroid metabolites. In addition, progesterone and histone deacetylase (HDAC) inhibitors have also been reported to promote the glial cell differentiation with the enhancement of serotonin-stimulated brain-derived neurotrophic factor gene transcription through the production of 5alpha-reduced neurosteroids, thus suggesting that glial cell differentiation is probably implicated in the protection and survival of neuronal cells in the brain. Therefore, the expression of 5alpha-R gene in glial cells seems physiologically important in maintaining the neural function in the brain, but little is known about the mechanism underlying the regulation of 5alpha-R gene transcription. In the present study, the effect of a HDAC inhibitor trichostatin A (TSA) on 5alpha-R gene transcription in the glioma cells was examined, and TSA was shown to induce the elevation of 5alpha-R mRNA levels through the activation of the 5alpha-R promoter via a mechanism involving Sp1 and Sp3 transcription factors in a time- and concentration-dependent manner. Thus, both Sp1 and Sp3 are considered to play a physiological role in the regulation of 5alpha-R gene expression, and hence the production of 5alpha-reduced neurosteroids in glial cells.</P>

Poster Session : Translocation and transactivation of glucocorticoid receptor by mifepristone

( Song Her ) 한국생화학분자생물학회 (구 한국생화학회) 2006 생화학분자생물학회 춘계학술발표논문집 Vol.2006 No.-

Delayed recovery of a patient with obstructive azoospermia and a history of acute epididymitis

Song, Seung-Hun,Shim, Jeong Yun,Sung, Suye,Her, Young Sun,Oh, Mihee,Shin, Dong Hyuk,Lee, Jinil,Baek, Jeoungwon,Lee, Woo Sik,Kim, Dong Suk The Korean Society for Reproductive Medicine 2019 Clinical and Experimental Reproductive Medicine Vol.46 No.2

Obstructive azoospermia caused by acute epididymitis is usually permanent, and microsurgical vasoepididymostomy is the only reconstructive treatment option. There have been no reports of delayed recovery of sperm count after over 1 year in a patient with obstructive azoospermia related to history of acute epididymitis. We present a young male patient who had azoospermia and a history of acute epididymitis who experienced delayed recovery, with complete restoration of sperm production and the ability to conceive naturally.

창원지역 일부 초등학교 고학년 학생의 전통발효식품에 대한 기호도와 영양지식 조사연구

송양순,허은실,이경화 창원대학교 생활과학연구소 2000 생활과학연구 Vol.4 No.-

The purpose of this study was to investigate Food Preference and Nutritional Knowledge for Traditional Fermented foods that was basic data to develope nutrition education program and to increase consumption of traditional fermented foods. The questionnaires have been collected from 229 fourth, fifth and sixth grade students of one elementary in Changwon. The results of this study were summarized as follows. 70.1% of students answered that they like ganjang, and that was higher in male compared to female(p<0.05). 82.2% of students answered that they like kochujang, and the hot taste of the kochujang was main reason of their like of kochujang and it was the most important reason for those also dislike of kochujang. 57.4% of students like deonjang, and that was higher in male than female same as ganjang(p<0.05). The reason for favorite deonjang was 'superior taste', 'great nutrient' and 'frequent consumption', and that was significantly defferent between gender. 84.2% of students answered that they like kimchi. The main reason for favorite kimchi was same as that of deonjang, and that dislike kimchi was 'hot taste' and 'inferior taste'. The student's most favorite food cooked with kimchi were stir fried kimchi rice and kimchi stew. The Student wanted taste of kimchi which was mild hot, hot and mild sweet, 85.6% of students prefer sikhe. The main reason for like sikhe was 'superior taste' and that dislike sikhe was 'inferior taste'. Most parents used to traditional fermented foods in meal preparation. The mean of nutrition knowledge was 6.03, and that was not significantly defferent between gender. Consequently these date suggest that education program to increase of nutrition knowledge and consumption of traditional fermented food is required for elementary school students.

<i>Oenanthe Javanica</i> Extract Protects Mouse Skin from UVB Radiation via Attenuating Collagen Disruption and Inflammation

Her, Young,Shin, Bich-Na,Lee, Yun Lyul,Park, Joon Ha,Kim, Dae Won,Kim, Ki Seob,Kim, Hyunjung,Song, Minah,Kim, Jong-Dai,Won, Moo-Ho,Ahn, Ji Hyeon MDPI 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.6

<P>In recent years, the use of botanical agents to prevent skin damage from solar ultraviolet (UV) irradiation has received considerable attention. <I>Oenanthe javanica</I> is known to exert anti-inflammatory and antioxidant activities. This study investigated photoprotective properties of an <I>Oenanthe javanica</I> extract (OJE) against UVB-induced skin damage in ICR mice. The extent of skin damage was evaluated in three groups: control mice with no UVB, UVB-exposed mice treated with vehicle (saline), and UVB-exposed mice treated with 1% extract. Photoprotective properties were assessed in the dorsal skin using hematoxylin and eosin staining, Masson trichrome staining, immunohistochemical staining, quantitative real-time polymerase chain reaction, and western blotting to analyze the epidermal thickness, collagen expression, and mRNA and protein levels of type I collagen, type III collagen, and interstitial collagenases, including matrix metalloproteinase (MMP)-1 and MMP-3. In addition, tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-2 protein levels were also assessed. In the UVB-exposed mice treated with extract, UV-induced epidermal damage was significantly ameliorated. In this group, productions of collagen types I and III were increased, and expressions of MMP-1 and MMP-3 were decreased. In addition, TNF-α and COX-2 expressions were reduced. Based on these findings, we conclude that OJE displays photoprotective effects against UVB-induced collagen disruption and inflammation and suggest that <I>Oenanthe javanica</I> can be used as a natural product for the treatment of photodamaged skin.</P>

흔적 자궁각 임신 1예

허광현,남상욱,박일영,배두진,김경홍,송재경,이종학 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.2

Pregnancy in rudimentary uterine horn is very rate. We experienced a case of rudimentary uterine horn pregnancy with 15 week gestation. she was cured by excision of rudimentary uterine born and pertinent reference were reviewed briefly.

Inhibition of tumor progression by oral piceatannol in mouse 4T1 mammary cancer is associated with decreased angiogenesis and macrophage infiltration

Song, H.,Jung, J.I.,Cho, H.J.,Her, S.,Kwon, S.H.,Yu, R.,Kang, Y.H.,Lee, K.W.,Park, J.H.Y. Butterworths ; Elsevier Science Ltd 2015 The Journal of nutritional biochemistry Vol.26 No.11

Piceatannol, a polyphenol which exhibits anticancer activities, is found in grapes, red wine and berries. It has been shown to inhibit several transcription factor pathways. The present study was conducted to determine whether oral administration of piceatannol inhibits mammary tumor progression. 4T1 mammary carcinoma cells were injected into the mammary fat pad of syngeneic female BALB/c mice. Starting 1 day later, piceatannol (10- or 20-mg/kg body weight/day) was administered by oral gavage for 30 days. Piceatannol treatment reduced tumor growth. In tumor tissues, piceatannol treatment reduced the expression of transcription factors P-NFκB p65, P-STAT3 and HIF-1α and multiple proteins involved in regulation of cell cycle progression (Ki67, cyclin D1, cyclin A, CDK2, CDK4), angiogenesis (VEGF-A, VEGFR-2, VE-cadherin, CD31) and lymphangiogenesis (VEGF-C, LYVE-1), as well as macrophage infiltration. Piceatannol significantly increased apoptotic cells and expression of both Bax and cleaved caspase-3 but reduced Bcl-2 expression in tumor tissues. In addition, piceatannol reduced the number and volume of pulmonary tumor nodules and expression of MMP-9 in both lung and tumor. It also reduced tissue levels of cytokines/chemokines, including M-CSF and MCP-1. In vitro results revealed that piceatannol inhibited migration of 4T1 cells and monocytes, as well as secretion of MCP-1 and M-CSF by 4T1 cells. 4T1 cell-conditioned medium stimulated monocyte migration, which was suppressed by a CCR2 antibody. These results indicate that alteration in tumor microenvironment (macrophages, transcription factors, etc.) is an important mechanism by which piceatannol inhibits tumor proliferation, angiogenesis and lymphangiogenesis, leading to suppression of mammary tumor growth and metastasis.

A Clinical Risk Score to Predict In-hospital Mortality from COVID-19 in South Korea

Her Ae-Young,Bhak Youngjune,Jun Eun Jung,Yuan Song Lin,Garg Scot,Lee Semin,Bhak Jong,Shin Eun-Seok 대한의학회 2021 Journal of Korean medical science Vol.36 No.15

Background: Early identification of patients with coronavirus disease 2019 (COVID-19) who are at high risk of mortality is of vital importance for appropriate clinical decision making and delivering optimal treatment. We aimed to develop and validate a clinical risk score for predicting mortality at the time of admission of patients hospitalized with COVID-19. Methods: Collaborating with the Korea Centers for Disease Control and Prevention (KCDC), we established a prospective consecutive cohort of 5,628 patients with confirmed COVID-19 infection who were admitted to 120 hospitals in Korea between January 20, 2020, and April 30, 2020. The cohort was randomly divided using a 7:3 ratio into a development (n = 3,940) and validation (n = 1,688) set. Clinical information and complete blood count (CBC) detected at admission were investigated using Least Absolute Shrinkage and Selection Operator (LASSO) and logistic regression to construct a predictive risk score (COVID-Mortality Score). The discriminative power of the risk model was assessed by calculating the area under the curve (AUC) of the receiver operating characteristic curves. Results: The incidence of mortality was 4.3% in both the development and validation set. A COVID-Mortality Score consisting of age, sex, body mass index, combined comorbidity, clinical symptoms, and CBC was developed. AUCs of the scoring system were 0.96 (95% confidence interval [CI], 0.85–0.91) and 0.97 (95% CI, 0.84–0.93) in the development and validation set, respectively. If the model was optimized for > 90% sensitivity, accuracies were 81.0% and 80.2% with sensitivities of 91.7% and 86.1% in the development and validation set, respectively. The optimized scoring system has been applied to the public online risk calculator (https://www.diseaseriskscore.com). Conclusion: This clinically developed and validated COVID-Mortality Score, using clinical data available at the time of admission, will aid clinicians in predicting in-hospital mortality.

Misuse of testosterone replacement therapy in men in infertile couples and its influence on infertility treatment

Song, Seung-Hun,Sung, Suye,Her, Young Sun,Oh, Mihee,Shin, Dong Hyuk,Lee, Jinil,Baek, Jeongwon,Lee, Woo Sik,Kim, Dong Suk The Korean Society for Reproductive Medicine 2019 Clinical and Experimental Reproductive Medicine Vol.46 No.4

Objective: We investigated the clinical characteristics of men with testosterone replacement therapy (TRT)-induced hypogonadism and its effect on assisted reproductive technology (ART) in infertile couples. Methods: This study examined the records of 20 consecutive male patients diagnosed with azoospermia or severe oligozoospermia (< 5 × 10⁶/mL) who visited a single infertility center from January 2008 to July 2018. All patients were treated at a primary clinic for erectile dysfunction or androgen deficiency symptoms combined with low serum testosterone. All men received a phosphodiesterase 5 inhibitor and TRT with testosterone undecanoate (Nebido^®) or testosterone enanthate (Jenasteron^®). Patients older than 50 years or with a chronic medical disease such as diabetes were excluded. Results: The mean age of patients was 37 years and the mean duration of infertility was 16.3 ± 11.6 months. At the initial presentation, eight patients had azoospermia, nine had cryptozoospermia, and three had severe oligozoospermia. Serum follicle-stimulating hormone levels were below 1.0 mIU/mL in most patients. Three ongoing ART programs with female factor infertility were cancelled due to male spermatogenic dysfunction; two of these men had normal semen parameters in the previous cycle. After withholding TRT, serum hormone levels and sperm concentrations returned to normal range after a median duration of 8 months. Conclusion: TRT with high-dose testosterone can cause spermatogenic dysfunction due to suppression of the hypothalamic-pituitary-testicular axis, with adverse effects on infertility treatment programs. TRT is therefore contraindicated for infertile couples attempting to conceive, and the patient's desire for fertility must be considered before initiation of TRT in a hypogonadal man.