The m6A reader IGF2BP3 promotes acute myeloid leukemia progression by enhancing RCC2 stability

Zhang Nan,Shen Yan,Li Huan,Chen Ying,Zhang Ping,Lou Shifeng,Deng Jianchuan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

N6-methyladenosine (m6A) is the most abundant posttranscriptional modification of mRNA in eukaryotes. Recent evidence suggests that dysregulated m6A-associated proteins and m6A modifications play a pivotal role in the initiation and progression of diseases such as cancer. Here, we identified that IGF2BP3 is specifically overexpressed in acute myeloid leukemia (AML), a subtype of leukemia associated with poor prognosis and high genetic risk. IGF2BP3 is required for maintaining AML cell survival in an m6A-dependent manner, and knockdown of IGF2BP3 dramatically suppresses the apoptosis, reduces the proliferation, and impairs the leukemic capacity of AML cells in vitro and in vivo. Mechanistically, IGF2BP3 interacts with RCC2 mRNA and stabilizes the expression of m6A-modified RNA. Thus, we provided compelling evidence demonstrating that the m6A reader IGF2BP3 contributes to tumorigenesis and poor prognosis in AML and can serve as a target for the development of cancer therapeutics.

Novel Organic/Inorganic Hybrid Star Polymer Surface-Crosslinked with Polyhedral Oligomeric Silsesquioxane

Jingyan Zhang,Dong Si,Shifeng Wang,Hao Liu,Xiaoming Chen,Haiou Zhou,Mingdi Yang,Guoying Zhang 한국고분자학회 2020 Macromolecular Research Vol.28 No.2

Novel organic/inorganic hybrid star polymer was prepared dually crosslinked within inner-core via divinylbenzene (DVB) and outer-surface via octafunctional polyhedral oligomeric silsesquioxane (POSS). Core cross-linked star polymers bearing dialkynyl-terminated polystyrene arms, (dialkynyl-PS)n-CCL, were synthesized at first by the atom transfer radical polymerization (ATRP) of DVB using α,α-dialkynyl-terminated PS macroinitiator, followed by the subsequent fractionation. Under high dilution conditions, (dialkynyl-PS)n-CCL was subjected to surface cross-linking with octa(3- azidopropyl) polyhedral oligomeric silsesquioxane, POSS-(N3)8, via click reaction, affording POSS-functionalized hybrid polymer doubly cross-linked within core and surface regions, SCL-(PS)n-CCL. FT-IR, 1H NMR, GPC, and elemental analysis results revealed that on average, the obtained hybrid polymer possesses a cross-linked PDVB inner core, ~14 linear PS arms (the MW per arm of 5.1 kDa), and ~4-5 POSS moieties at outer surface. Differential scanning calorimetry (DSC) thermograms and thermogravimetric analysis (TGA) revealed that after surface cross-linking the thermal stability of SCL-(PS)n-CCL is considerably improved. This work provides a proof-of-concept example for the preparation of dually cross-linked hybrid star polymer, which represents a novel category of organic/inorganic composite materials with unique chain architectures.

Double 2-dimensional H₂-evoluting catalyst tipped photocatalyst nanowires: A new avenue for high-efficiency solar to H₂ generation

Zhang, Kan,Qian, Shifeng,Kim, Wanjung,Kim, Jung Kyu,Sheng, Xiaowei,Lee, Jun Young,Park, Jong Hyeok Elsevier 2017 Nano energy Vol.34 No.-

<P><B>Abstract</B></P> <P>Asymmetric or symmetric metal-tipped one-dimensional (1D) semiconductors are promising systems for efficient photocatalytic reactions such as solar-to-fuel conversion because of ultrafast exciton dynamics that arise at the specific heterostructure interface. However, synthesizing such unique nanostructures experiencing colloid growth on noble metal that has faced a formidable challenge in practical application because these synthesis conditions are not suitable to deploy in mass production. Here, we report the gram-scale mass production of symmetric MoS<SUB>2</SUB>-tipped CdS nanowires (S-MtC NWs) via edge-terminated attachment in a binary solvent. The factors influencing the formation of symmetric heterostructures are investigated by varying the types of precursors, initial concentration, and solvent composition. Under visible-light irradiation (λ≥420nm), the S-MtC NWs exhibit superior photocatalytic H<SUB>2</SUB> evolution activity (12.6mmol/g/h) compared to common Pt/CdS NWs (2.6mmol/g/h), corresponding to an apparent quantum yield of 37.6% at 420nm. This impressive photocatalytic ability is ascribed to spatially separated redox-active sites in the S-MtC NWs, in which the reduction and oxidation sites are at the MoS<SUB>2</SUB> tip and the CdS stem, respectively. Additionally, it is found that the length of CdS NWs as higher aspect ratio as possible could get better photocatalyst H<SUB>2</SUB> performance. The symmetry of 2D MoS<SUB>2</SUB> tips and 1D CdS NWs may provide advanced avenues for specific co-catalyst decoration, enabling co-catalysts to be selectively located at reduction or oxidation sites for other targeted solar artificial syntheses.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Symmetric 2D tipped 1D heterostructured nanowires were synthesized. </LI> <LI> The tipped structure exhibited the spatially separated redox-active sites. </LI> <LI> Theoretical simulation demonstrated one order of magnitude decrease in energy barrier. </LI> <LI> The photocatalytic performance of symmetric heterostructure was superior to asymmetric counterpart. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Symmetric 2D tipped 1D heterostructured nanowires synthesized by edge-terminated attachment exhibit efficient spatial charge separation effect and the longer the better photocatalytic activity, resulting in photocatalytic H<SUB>2</SUB> activity of 12.6mmol/h·g with excellent long-term durability (>10h) without additional passivation under visible light (λ≥420nm).</P> <P>[DISPLAY OMISSION]</P>

Let-7c miRNA Inhibits the Proliferation and Migration of Heat-Denatured Dermal Fibroblasts Through Down-Regulating HSP70

Shifeng Wu,Tao Jiang,Xingang Wang,Weiwei Wu,Fan Zhang 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.4

Wound healing is a complex physiological process necessitating the coordinated action of various cell types, signals and microRNAs (miRNAs). However, little is known regarding the role of miRNAs in mediating this process. In the present study, we show that let-7c miRNA is decreased in heat-denatured fibroblasts and that inhibiting let-7c expression leads to the increased proliferation and migration of dermal fibroblasts, whereas the overexpression of let-7c exerts an opposite effect. Further investigation has identified heat shock protein 70 as a direct target of let-7c and has demonstrated that the expression of HSP70 in fibroblasts is negatively correlated with let-7c levels. Moreover, down-regulation of let-7c expression is accompanied by up-regulation of Bcl-2 expression and down-regulation of Bax expression, both of which are the downstream genes of HSP70. Notably, the knockdown of HSP70 by HSP70 siRNA apparently abrogates the stimulatory effect of let-7c inhibitor on heat-denatured fibroblasts proliferation and migration. Overall, we have identified let-7c as a key regulator that inhibits fibroblasts proliferation and migration during wound healing.

Defect-Induced Epitaxial Growth for Efficient Solar Hydrogen Production

Zhang, Kan,Kim, Jung Kyu,Park, Bumsu,Qian, Shifeng,Jin, Bingjun,Sheng, Xiaowei,Zeng, Haibo,Shin, Hyunjung,Oh, Sang Ho,Lee, Chang-Lyoul,Park, Jong Hyeok American Chemical Society 2017 Nano letters Vol.17 No.11

<P>Epitaxial growth suffers from the mismatches in lattice and dangling bonds arising from different crystal structures or unit cell parameters. Here, we demonstrate the epitaxial growth of 2D MoS2 ribbon on 1D CdS nanowires (NWs) via surface and subsurface defects. The interstitial Cd-0 in the (1(2)over-bar10) crystal plane of the [0001]-oriented CdS NWs are found to serve as nucleation sites for interatomically bonded [001]-oriented MoS2, where the perfect lattice match (similar to 99.7%) between the(10(1)over-bar1) plane of CdS and the (002)-faceted in-plane MoS2 result in coaxial MoS2 ribbon/CdS NWs heterojunction. The coaxial but heterotropic epitaxial MoS2 ribbon on the surface of CdS NWs induces delocalized interface states that facilitate charge transport and the reduced surface state. A less than 5-fold ribbon width of MoS2 as hydrogen evolution cocatalyst exhibits a similar to 10-fold H-2 evolution enhancement than state of the art Pt in an acidi electrolyte, and apparent quantum yields of 79.7% at 420 nm 53.1% at 450 nm, and 9.67% at 520 nm, respectively.</P>

An In situ Forming Hydrogel Based on Photo-Induced Hydrogen Bonding

Jingyan Zhang,Shifeng Wang,Zeren Zhao,Dong Si,Haiou Zhou,Mingdi Yang,Xianbiao Wang 한국고분자학회 2020 Macromolecular Research Vol.28 No.12

Stimulus-induced in situ forming hydrogels possess the characteristics of easy management and minimal invasiveness via simple injection at target sites with a liquid and easy forming bulk gels. In the present study, a photoreactive monomer, N'-(2-nitrobenzyl)-N-acryloyl glycinamide (NBNAGA) was introduced to modify polyacrylamide (PAM) hydrogel preparation with stimuli responsiveness. Firstly, poly(acrylamide-co-N'-(2-nitrobenzyl)-N-acryloyl glycinamide), P(AM-co-NBNAGA), copolymer solution was prepared via reversible addition fragmentation chain transfer (RAFT) polymerization using the monomers mixture of AM, NBNAGA, and N,N'- methylene bis-acrylamide (BIS). The obtained polymer solution with viscous, transparent, and flowable appearance contained weak single hydrogen bonding and slight chemical crosslinking in a microscopic perspective. Secondly, without further purification, after irradiation using UV light at 365 nm, poly(acrylamide-co-N-acryloyl glycinamide) (P(AM-co-NAGA)) hydrogel conveniently in situ formed due to the cleavage of o-nitrobenzyl groups and the corresponding emergency of dual hydrogen bonding among “uncaged” dual amide moieties. P(AM-co-NAGA) hydrogel depicted both favorable temperature sensitivity and self-healing properties, then the heating induced in vitro release profiles of doxorubicin (DOX) was analyzed.

Curcumin represses lipid accumulation through inhibiting ERK1/2-PPAR-γ signaling pathway and triggering apoptosis in porcine subcutaneous preadipocytes

Pan, Shifeng,Chen, Yongfang,Zhang, Lin,Liu, Zhuang,Xu, Xingyu,Xing, Hua Asian Australasian Association of Animal Productio 2022 Animal Bioscience Vol.35 No.5

Objective: Excessive lipid accumulation in adipocytes results in prevalence of obesity and metabolic syndrome. Curcumin (CUR), a naturally phenolic active ingredient, has been shown to have lipid-lowering effects. However, its underlying mechanisms have remained largely unknown. Therefore, the study aims to determine the effect of CUR on cellular lipid accumulation in porcine subcutaneous preadipocytes (PSPA) and to clarify novel mechanisms. Methods: The PSPA were cultured and treated with or without CUR. Both cell counting Kit-8 and lactate dehydrogenase release assays were used to examine cytotoxicity. Intracellular lipid contents were measured by oil-red-o staining extraction and triglyceride quantification. Apoptosis was determined by flow cytometry and the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling assay. Adipogenic and apoptosis genes were analyzed by quantitative polymerase chain reaction and Western blot. Results: The CUR dose-dependently reduced the proliferation and lipid accumulation of PSPA. Noncytotoxic doses of CUR (10 to 20 μM) significantly inhibited extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation and expression of adipogenic genes peroxisome proliferation-activity receptor-γ (PPAR-γ), CCAAT/enhancer binding protein-α, sterol regulatory element-binding protein-1c, adipocyte protein-2, glucose transporter-4 as well as key lipogenic enzymes fatty acid synthase and acetyl-CoA carboxylase, while ERK1/2 activation significantly reversed CUR-reduced lipid accumulation by increasing PPAR-γ. Furthermore, compared with differentiation induced media treated cells, higher dose of CUR (30 μM) significantly decreased the expression of AKT and B-cell lymphoma-2 (BCL-2), while increased the expression of BCL-2-associated X (BAX) and the BAX/BCL-2 expression ratio, suggesting triggered apoptosis by inactivating AKT and increasing BAX/BCL-2 ratio and Caspase-3 expression. Moreover, AKT activation significantly rescued CUR inhibiting lipid accumulation via repressing apoptosis. Conclusion: These results demonstrate that CUR is capable of suppressing differentiation by inhibiting ERK1/2-PPAR-γ signaling pathway and triggering apoptosis via decreasing AKT and subsequently increasing BAX/BCL-2 ratio and Caspase-3, suggesting that CUR provides an important method for the reduction of porcine body fat, as well as the prevention and treatment of human obesity.

C-Band Synthetic Aperture Microwave Radiometer and Its Imaging Experiments

Shengwei, Zhang,Xiaolong, Dong,Ji, Wu,Jingshan, Jiang,Shifeng, Zheng 대한원격탐사학회 2001 International Symposium on Remote Sensing Vol.17 No.1

The principles of the interferometric synthetic aperture was been briefly introduced, the system design of a C-band microwave interferometric synthetic aperture radiometer is presented. This systemhas a 6-element thinned array with 11 analog complex correlator to obtain the 11 visibility function spatial- frequency terns, which result in 22 digitally synthesizedspatial resolution cell. So the synthetic apertureradiometer have a spatial resolution as high as one 22nd of that corresponding to one antenna element. Thesynthetic radiometer has a real aperture resolution cell in the along-hack direction and has 22 synthetic apertureresolution cells in the cross-track direction. Finally, the results of imaging experiments are given.

The Structure-preserving Doubling Numerical Algorithm of the Continuous Coupled Algebraic Riccati Equation

Juan Zhang,Shifeng Li 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.7

In the paper, we apply a structure-preserving doubling algorithm to solve the continuous coupled algebraic Riccati equation (CCARE). Using the existence and uniqueness of the CCARE, we show that the iteration solution of the CCARE are positive semi-definite, symmetric, and unique. Further, we discuss the convergent analysis of the structure-preserving doubling algorithm. Moreover, we present two modified structure-preserving doubling algorithms. Finally, we offer corresponding numerical examples to illustrate the effectiveness of the derived numerical algorithms.

Physicochemical properties enhancements of soy protein isolate-based films by the hybrid tri-functional aziridines and carboxylated styrene-butadiene rubber latex

Haijiao KANG,Shifeng ZHANG 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8