의약품중 잔류유기용매 시험법 확립에 관한 연구

고용석,강찬순,최보경,김혜수,홍정희,최명신,김상현,장성재,김길수 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

Headspace-GC(딘5-GC)를 이용하여 ICH 잔류웅매 가이드라인 class 1, 2에 속하는 28종의 잔류유기웅매 분석방법을 연군한 결과 SPB-5, BB-WAX column을 동시 이용하여 각각을 확인, 정량할 수 있었다.또한 염의 참가효과, 평형시간,평형온도가 headspace분석에 미치는 영향을 검토한 결과 평형 시간 30분,평형온도 85℃,Na₂SO₄Ig을 첨가하여 시험할 때 최적 부넉조건을 나타내었다. 회수율은 1,1-achloroethene(68.3%)를 제외하고 90.9 ~ 114.5%로 패체적으로 양호하였다. 따로 HS-GCJECD, DB-524 column을 이용하여 9종의 잔류유기용매에 대하여 각각의 검랸선을 작성하고 검출한계를 구하였다. 실제 의약품 원료 10종및 제제 5종에 적용시킨 결과 모두 불검출되었으며 본 시험법은 의약품중의 잔류유기용매 분석에 적용할 수 있는 방법이라고 판단된다. The headspace-fC/FID(HS -GC/FID) method was performed for test method developnlent of residu;31 organic solvents in pfrarmaceuticals. Using SPB-S and BB-WAXcolumn, 28 kinds of solvents iri ICH residual sof)·ents guidetine class 1, 2 couBd be individuallt-identified and quantitated. The foIBowing residoal soIYents were not detected by the headspaceinjection condition : N,N-Cmethylacetamide, N,N-dimeth)rlformamide, ethyfeneglycol, formamide,2-methoryethanot, N-rnethylpyrrolidone, suBforane. The effects of the addition of salts,equilibration time, and equilibration temperature on headspace analt·sis were invesugated. Theoptimum conditions weiFe obtained with addtion of Ifa2s04 19 as a salt, simultaneously. thetime and temperature of equitibration were 30min and 8i'f, respectivety. The recovery havefound between 90.9 and 114.5% except 1,1-dichloroethene(fi8.3%). Using DB-624 column &HS-GC/:ECD method, 9 kinds of residual solvents could be individually identified andfuantitated. This HS-GC method can be applied to test the residua3 organic solvent in thepharmaceuticals.

Facial Redness in Atopic Dermatitis Patients Treated With Dupilumab: A Case Series

Seung Hui Seok,Ji Hae An,Jung U Shin,Hee Jung Lee,Dong Hyun Kim,Moon Soo Yoon,Hyun Jung Kim 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.6

Analysis of Vulvar Dermatoses Presenting as Vulvar Pruritus: A Single-Center Retrospective Study

( Seung Hui Seok ),( Jung U Shin ),( Dong Hyun Kim ),( Moon Soo Yoon ),( Hee Jung Lee ) 대한피부과학회 2021 大韓皮膚科學會誌 Vol.59 No.7

Background: Vulvar pruritus is a common complaint among women presenting to dermatologists. However, few studies have analyzed the dermatologic conditions that cause it. Objective: This retrospective study aims to evaluate the clinical features and causes of pruritic skin lesions of the female external genitalia. Methods: This study included 161 female patients with vulvar pruritus between 2008 and 2018 at CHA Bundang Medical Center. Data were collected by reviewing the electronic medical records retrospectively. The age, diagnosis, and histopathologic findings of the patients were reviewed. Results: The patients’ mean age was 49 years. On physical examination, 71.4% of patients (n=115) had definite skin lesions, and 28.6% (n=46) had ‘vulvar pruritus without skin rash’. The most common diagnostic category, confirmed by skin biopsy, was inflammatory dermatoses (53.4%, n=86), including lichen sclerosus et atrophicus, lichen simplex chronicus, nonatopic eczema, atopic eczema, and psoriasis. Moreover, 7.5% of patients (n=12) were diagnosed with infectious diseases, including candidiasis, herpes simplex virus, syphilis, and scabies; 5.6% (n=9) were diagnosed with neoplastic diseases, including vulvar intraepithelial neoplasia, squamous cell carcinoma, extramammary Paget’s disease, and Bowen’s disease. Conclusion: The causes of vulvar itch are vast, and often, multiple causes coexist simultaneously. Therefore, it requires a systemic approach to establish the correct diagnosis. Dermatologists should actively participate in the diagnosis and treatment. (Korean J Dermatol 2021;59(7):527∼533)

Analysis of vulvar dermatoses presenting as vulvar pruritus: A single-center retrospective study

( Seung Hui Seok ),( Go Woon Choi ),( Ji Hae An ),( Seon Gu Lee ),( Jung U Shin ),( Hyun Jung Kim ),( Dong Hyun Kim ),( Moon Soo Yoon ),( Hee Jung Lee ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2

Background: Vulvar pruritus is a common complaint among women presenting to gynecologists and dermatologists. Nonetheless there have been few studies analyzing dermatologic conditions which caused vulvar pruritus. Objectives: This study aimed to evaluate the clinical features and causes of pruritic skin lesions of female external genitalia. Methods: In this study, 161 patients with vulvar pruritus between October 2008 and September 2018 at Bundang CHA hospital were included. Data were collected by reviewing the electronic medical records retrospectively. The age, diagnosis, histopathologic findings, and treatments of the patients were reviewed. Results: In total, 161 patients over a 10-years period were included. Patients ranged in age from 3 to 89 years (mean age: 49±19.27 years) old. On physical examination, 74.5% of patients (n=120) had definite skin lesions and 25.5% of patients (n=41) were classified as true pruritus vulvae (pruritus without skin rash). The most common diagnostic category confirmed by skin biopsy was inflammatory dermatoses (56.5%, N=91). 7.5% of patients (n=12) were diagnosed with infectious diseases and 5.6% of patients (n=9) were diagnosed with neoplastic diseases. Conclusion: Causes of vulvar itch are vast and may be inflammatory, environmental, neoplastic, or infectious, often with several causes coexisting simultaneously. So it requires a systemic approach to establish the correct diagnosis. Dermatologists should actively participate in the diagnosis and treatment.

A case of sarcoidosis associated with nontuberculous mycobacteria infection

( Seung Hui Seok ),( Go Woon Choi ),( Ji Hae An ),( Seon Gu Lee ),( Jung U Shin ),( Hyun Jung Kim ),( Hee Jung Lee ),( Moon Soo Yoon ),( Dong Hyun Kim ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.1

Sarcoidosis is a multisystem granulomatous disease of unknown cause. The lung is the most commonly affected organ, but the skin is frequently involved. Its etiology is unknown, though a range of factors including tuberculous infection, beryllium exposure and cold climate have been implicated in the pathogenesis of the condition. Both Mycobacterium tuberculosis and nontuberculous mycobacteria (NTM) have been implicated in sarcoidosis pathogenesis. Despite this, the role of mycobacteria in sarcoidosis remains unclear. A 45-year-old female presented with a 4-month history of an asymptomatic eye lid swelling and multiple erythematous papules on the neck. Computed tomography image showed a hypertrophy in bilateral lacrimal glands. Histological examination of the neck, orbital soft tissue and lacrimal gland revealed non-necrotizing granuloma consistent with sarcoidosis. Zielh-Neelsen stains and polymerase chain reactions (PCRs) for tuberculosis (TB) in skin biopsy specimens were negative. But NTM PCR was positive in the orbital soft tissue specimen. The patient was referred to rheumatologist and was finally diagnosed with sarcoidosis associated with NTM. Treatment with systemic steroid and hydroxychloroquine was started, resulting in an improvement of skin lesions. We herein report a case of sarcoidosis associated with NTM infection with review of the literature, as little is known regarding the role of mycobacteria in sarcoidosis.

Lupus vulgaris mimicking tinea corporis

( Seung Hui Seok ),( Moon Soo Yoon ),( Dong Hyun Kim ) 대한피부과학회 2021 대한피부과학회 학술발표대회집 Vol.72 No.2

A case of primary inoculation tuberculosis

( Seung Hui Seok ),( Go Woon Choi ),( Ji Hae An ),( Seon Gu Lee ),( Jung U Shin ),( Hyun Jung Kim ),( Hee Jung Lee ),( Dong Hyun Kim ),( Moon Soo Yoon ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.1

Primary inoculation tuberculosis (TB) is a rare form of cutaneous TB resulting from the direct inoculation of Mycobacterium tuberculosis (M. tuberculosis) into the skin or mucosa of a previously uninfected person or non-immune person. The pathogenesis requires that the skin be damaged by abrasions or injuries that allow penetration of the tubercle bacilli. A 61-year-old female presented with a 1-month history of multiple erythematous nodules on her left arm and dorsum of hand. There was no history of trauma in the vicinity of the lesion. Histologic findings showed granulomatous inflammatory infiltration and acid-fast bacilli. M. tuberculosis was identified by polymerase chain reaction (PCR), but a culture showed no growth. The diagnosis of primary inoculation TB was made based on clinical, histological features. Six months after the initiation of anti-tuberculosis medication, skin lesions were improved, and there was no evidence of recurrence clinically or histologically. We herein report a case of primary inoculation TB that emphasizes the importance of clinical suspicion of tuberculosis and the importance of biopsy confirmation even if there is no specific history.

Molluscum contagiosum on the sole

( Seung Hui Seok ),( Moon Soo Yoon ),( Jung U Shin ) 대한피부과학회 2021 대한피부과학회 학술발표대회집 Vol.72 No.2

A case of solitary mycosis fungoides on the scrotum

( Seung Hui Seok ),( Go Woon Choi ),( Ji Hae An ),( Seon Gu Lee ),( Jung U Shin ),( Hyun Jung Kim ),( Hee Jung Lee ),( Dong Hyun Kim ),( Moon Soo Yoon ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.1

Mycosis fungoides (MF) is the most common cutaneous T-cell lymphoma (CTCL), accounting for almost 50% of all primary cutaneous lymphomas. The occurrence of solitary lesions, which are clinically and histopathologically indistinguishable from classic MF has been described. Clinically these solitary lesions present as a single, erythematous scaly patch, plaque, or nodule that vary in size and location. The lesions are usually found in body regions typical for classic MF, mainly none exposed areas. To our knowledge, only few cases have been reported as solitary MF in the genitalia. A 54-year-old man visited our clinic with a 5-6mm sized skin colored nodule on the scrotum which had found 2 months ago. There were no other skin lesions. A punch biopsy specimen revealed a prominent infiltrate of atypical lymphocytes in dermis with some epidermotropism and lining up of atypical lymphocytes along the junctional zone. Molecular analysis revealed a monoclonal rearrangement of the T-cell receptor (TCR) genes. The patient was diagnosed with solitary MF and referred to another hospital. We herein report a case of solitary MF on the scrotum which is an unusual area with review of the literature.

New Normal for Dermatology in AC (After COVID-19) Era

( Seung Hui Seok ),( Sekyoo Jeong ),( Hyun Jung Kim ) 한국피부장벽학회 2020 한국피부장벽학회지 Vol.22 No.1

Coronavirus disease 2019 (COVID-19) is currently in progress, affecting healthcare systems, education, and the economy as a whole, which have not been experienced around the world, and the dermatology and cosmetic industry is rapidly entering a new paradigm. In this review, we will examine what COVID-19, which has been basically regarded as a symptom of respiratory and fever infections to date, has various clinical manifestation of dermatology. In order to control the rapidly growing number of patients, all medical staff in the epidemic area are actively equipped with full PPE or sometimes continue to take care even with only gloves and a mask in a poor environment, which causes various skin damage and skin diseases. In the age of New Normal, everyone wearing masks and washing their hands also has faced to the skin diseases that non-medical people will face every day. This review also will cover up skin disease related to the face mask, gloves and hand washing. All the medical resources must be shared for COVID-19 crisis and dermatologist must reassign the previous process for our patients in new normal. Those strategies are the important issues to be covered in this review. Ultimately, we will talk about the effects of these unexpected viral infections on the future of dermatology in various aspects.