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Ha, Nguyen Thi Hoang,Ha, Nguyen Thi,Nga, Tran Thi Huyen,Minh, Nguyen Ngoc,Anh, Bui Thi Kim,Hang, Nguyen Thi An,Duc, Nguyen Anh,Nhuan, Mai Trong,Kim, Kyoung-Woong Elsevier 2019 Applied geochemistry Vol.108 No.-
<P><B>Abstract</B></P> <P>Phytoremediation is a plant-based, environment-friendly, and cost-effective technology that can be potentially used to remediate contaminated media. This study was conducted to evaluate the phytoextraction and phytostabilization potential, two common techniques of phytoremediation, of 21 plant species growing naturally at the largest tungsten (W) mine in Vietnam—the second largest production of W in the world. The average concentrations in different soil fractions were in the following order: residue > organically complexed, Fe- and Mn oxide-bound > carbonate bound > exchangeable. The total concentrations of arsenic (As), cadmium (Cd), copper (Cu), and zinc (Zn) in the soil varied by 34–3390, 4.87–81.6, 14.3–2080, and 21.9–370 mg/kg, respectively. The concentrations of As, Cd, Cu, and Zn in the shoots ranged 0.71–2400, 0.05–5.55, 4.81–249, and 13.3–380 mg/kg-DW, respectively. The concentrations of As and Cd in all soil samples and Cu in 70% of soil samples collected around the mine fluctuated within 2–227, 3–54, and up to 21-folds higher than the maximum allowable limit for agricultural soils in Vietnam (QCVN 03-MT:2015/BTNMT). The results indicated that hyperaccumulation levels (mg/kg-DW) were obtained for only As in <I>Pityrogramma calomelanos</I> (2400) and <I>Pteris vittata</I> L. (1860). Based on the recorded hyperaccumulation levels, translocation and accumulation factors, biomass, and fast growth of these plants, <I>P. calomelanos</I> and <I>P. vittata</I> were considered to be promising native plants for the phytoextraction of As contaminated soils. <I>Bidens pilosa</I> L. also has great potential for phytostabilization of mining soils contaminated with As and heavy metals.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Soils near multi-metal (W–F–Cu–Au–Bi) mine were contaminated with As, Cd, Cu. </LI> <LI> Arsenic in shoots were in order of Fern > Monocotyledonae > Dicotyledonae. </LI> <LI> <I>Pityrogramma calomelanos</I> and <I>Pteris vittata</I> L. suggested for phytoextraction of As. </LI> <LI> <I>Bidens pilosa</I> L. suggested for the phytostabilization of mining contaminated soils. </LI> </UL> </P>
Effects of Glycerol and Shikimic Acid on Rapamycin Production in Streptomyces rapamycinicus
( Huyen Thi Huong La ),( Thao Kim Nu Nguyen ),( Hang Thuy Dinh ),( Quyen Minh Huynh Nguyen ),( Minh Hong Nguyen ) 한국미생물 · 생명공학회 2020 한국미생물·생명공학회지 Vol.48 No.3
Rapamycin, derived from Streptomyces rapamycinicus, is an important bioactive compound having a therapeutic value in managing Parkinson’s disease, rheumatoid arthritis, cancer, and AIDS. Because of its pharmaceutical activity, studies over the past decade have focused on the biosynthesis of rapamycin to enhance its yield. In this study, the effect of rapG on rapamycin production was investigated. The rapG expression vector was constructed by utilizing the integration vector pSET152 under the control of the erythromycin resistance gene (ermE*), a strong constitutive promoter. The rapamycin yield of wild type (WT) and WT/ rapG overexpression mutant strains, under fermentation conditions, was analyzed by high-performance liquid chromatography (HPLC). Our results revealed that overexpression of rapG increased rapamycin production by approximately 4.9-fold (211.4 mg/l) in MD1 containing 15 g/l of glycerol, compared to that of the WT strain. It was also found that Illicium verum powder (10 g/l), containing shikimic acid, enhanced rapamycin production in both WT and WT/rapG strains. Moreover, the amount of rapamycin produced by the WT/rapG strain was statistically higher than that produced by the WT strain. In conclusion, the addition 15 g/l glycerol and 15 g/l I. verum powder produced the optimal conditions for rapamycin production by WT and WT/rapG strains.
Nguyen, Thi Huyen,Nguyen, Thi Minh Hien,Kang, Boyoun,Cho, Beongki,Park, Yeonju,Jung, Young Mee,Yang, In-Sang Elsevier 2018 Journal of molecular structure Vol.1165 No.-
<P><B>Abstract</B></P> <P>2D correlation analysis (2DCOS) and principal component analysis (PCA) have been performed on temperature-dependent Raman spectra from 12 K to 300 K of SmB<SUB>6</SUB> single crystals grown from Boron with a purity of 99.9% (3N) or 99.9999% (6N). Significant differences in the Raman spectra of SmB<SUB>6</SUB>(3N) and SmB<SUB>6</SUB>(6N) are observed. The Raman active T<SUB>2g</SUB>, E<SUB>g</SUB>, and A<SUB>1g</SUB> modes of SmB<SUB>6</SUB>(3N) are broader and shift to higher wavenumbers compared with those of SmB<SUB>6</SUB>(6N). The results of 2DCOS and PCA on temperature-dependent Raman spectra of SmB<SUB>6</SUB>(3N) and SmB<SUB>6</SUB>(6N) give us further information. We find that all the T<SUB>2g</SUB>, E<SUB>g</SUB>, and A<SUB>1g</SUB> modes of SmB<SUB>6</SUB>(6N) are single bands, while those of SmB<SUB>6</SUB>(3N) show all doublet features. The appearance of multiple bands in Raman vibrational modes of SmB<SUB>6</SUB>(3N) sample implies that the structure of boron octahedra of the SmB<SUB>6</SUB>(3N) has lower symmetry than that of SmB<SUB>6</SUB>(6N). Our findings in 2DCOS and PCA of the Raman spectra strongly support the differences in the behavior of SmB<SUB>6</SUB>(3N) and SmB<SUB>6</SUB>(6N).</P> <P><B>Highlights</B></P> <P> <UL> <LI> The symmetry of the boron octahedra in SmB<SUB>6</SUB> is found to be lowered by small amount of impurities in boron ingredient. </LI> <LI> Raman spectra of SmB<SUB>6</SUB> single crystals strongly depend on the Boron purity. </LI> <LI> 2DCOS and PCA show the multiple bands in the Raman modes of SmB6 made of 99.9% (3N) boron. </LI> </UL> </P>
Nguyen, Thanh Huong,Nguyen, Trung Kien,Kim, Bao Giang,Hoang, Van Minh,Phan, Thi Hai,Doan, Thu Huyen,Luong, Ngoc Khue,Nguyen, Thuy Linh,Nguyen, Tuan Lam,Pham, Thi Quynh Nga Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1
Studies have shown that smoking is a learnt behavior, often initiated during adolescence. This paper aims to describe tobacco-related knowledge, attitude and associations among school adolescents aged 13-15 with exposure to anti-smoking information. Using data from the Global Youth Tobacco Survey (GYTS) in Viet Nam, 2014, knowledge was measured through 4 questions about tobacco use, and attitude was assessed through 3 questions on personal, social and environmental aspects. Students giving most anti-tobacco responses to all questions were considered as having correct knowledge or appropriate attitude or both. Access to anti-smoking information was determined by exposure to any media messages on tobacco control during the past 30 days and teaching in school about the danger of tobacco use during the past 12 months. A substantial percentage of students thought that being near others who smoke might be harmful to them and smoking is harmful to health (89.4% and 89.6% respectively). However, only 46.4% reported that it is definitely difficult to quit smoking and 66.9% thought that smoking for only 1 or 2 years, once stopped, is harmful to health. Slightly more than half of the respondents reported appropriate attitude that young smokers have fewer friends than others and smoking makes them less attractive and less comfortable at social events. Noticing anti-smoking messages in the media together with having lessons in school about the dangers of tobacco substantially increased the likelihood of having correct knowledge, appropriate attitude and both. Despite relatively high awareness about smoking harms, effective educational communication is still highly needed to improve the level of comprehensive knowledge and an appropriate attitude regarding tobacco use.
Le, Thi Huong,Nga, Thi Thu Vu,Nguyen, Ngoc Dung,Le, Thi Thanh Xuan,Kim, Bao Giang,Phan, Thi Hai,Doan, Thu Huyen,Luong, Ngoc Khue,Nguyen, Tuan Lam,Hoang, Van Minh,Pham, Thi Quynh Nga Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1
The aim of this paper is to report the rate of current and ever cigarette smoking and explore correlates of current cigarette smoking among adolescents aged 13-15 in Viet Nam. This analysis was derived from GYTS survey, which comprised of 3,430 adolescents aged 13-15, conducted in 2014 in 13 cities and provinces of Viet Nam. We calculated the weighted rates of current and ever cigarette smoking and reported patterns of smoking behavior. We also performed logistic regression to explore correlates of current cigarette smoking behavior. The weighted rate of ever cigarette smoking was 9.5% (95% confidence interval (CI): 8.5 %-10.5%), in which the weighted rate among males (15.4%; 95% CI: 13.6%-17.0%) was higher than that among females (4.2%; 95% CI: 3.3%-5.1%). The weighted rate of current cigarette smoking was relatively low at 2.5% (95%CI: 2.0%-3.0%) with higher weighted rate among males (4.9%; 95% CI: 3.8%-5.9%) compared to the corresponding figure among females (0.2%; 95% CI: 0.0 %-0.5%). Current cigarette smoking was significantly higher among males than females, in students aged 15 versus 13 years old, and in students who had several or all close friends smoking and students with daily observation of smoking at school. For greater smoking reduction outcomes, we recommend that tobacco interventions for adolescents should consider targeting more male students at older ages, establish stricter adherence to school-based banning of cigarette smoking, engage both smoking and nonsmoking adolescents and empower adolescents to resist peer smoking influence as well as changing their norms or beliefs towards smoking benefits.
Le, Thi Kim Anh,Bui, Thi Tu Quyen,Hoang, Van Minh,Kim, Bao Giang,Phan, Thi Hai,Doan, Thu Huyen,Luong, Ngoc Khue,Nguyen, Tuan Lam,Pham, Thi Quynh Nga Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1
Efforts to reduce tobacco use among school children need to be based on understanding of access to cigarettes by these subjects because previous studies indicated that enforcement of laws for controlling tobacco sales seems to not affect teen/school children because they can obtain cigarettes from different sources. This paper aims to describe access to and availability of cigarettes among school students (aged 13-15 years old) according to the data from GYTS Vietnam 2014. In GYTS, a national school-based survey of students of grades 8-10, our findings showed that about 15% school children are current smokers who smoke at home, and that they could easily buy cigarettes from stores (63.2%), or someone else (27.8%), or street vendors (9%). Notably, over 85% of school children answered that they were not refused because of their age. This high percentage was nearly the same in the North (85.7%), the Centre (92.5%), and the South (89.7%) of Viet Nam. These findings show that it is quite easy for school children to obtain cigarettes and this is a crucial challenge for policy makers aiming to reduce tobacco use among youth in general and school-age students in particular.