디스크 레이저를 이용한 순 타이타늄의 비드용접시 비드색에 따른 용접특성

김지성(Ji-Sung Kim1),박수한(Su-Han Park),송무근(Moo-Keun Song),김종도(Jong-Do Kim) 한국마린엔지니어링학회 2018 한국마린엔지니어링학회지 Vol.42 No.7

요추 수술 예정 환자에서 실시한 선택적 신경근 차단술의 5년 추시 결과

심대무(Dae Moo Shim),김태균(Tae Kyun Kim),오성균(Sung Kyun Oh),최윤홍(Yun Hong Choi),이석중(Suk Jung Lee) 대한정형외과학회 2009 대한정형외과학회지 Vol.44 No.1

목적: 하지 방사통을 호소하는 환자의 수술 준비 과정에서 선택적 척추 신경근 차단술을 실시하고, 그 효과가 얼마나 오래 유지되는지를 알아 보고자 5년 이상 추시된 환자를 대상으로 분석하여 그 결과를 보고 하고자 한다. 대상 및 방법: 2000년 1월에서 2000년 12월까지 하지 방사통으로 타 병원 혹은 본원에서 수술적 치료를 권유받은 환자 중 수술 준비 과정에서 진단 및 치료 목적으로 선택적 척추 신경근 차단술을 시행한 환자를 대상으로 하였으며, 이중 5년 이상 추시가 가능하였던 69예를 대상으로 하였다. 결과분석은 진료기록부와 전화 인터뷰를 통해 하였으며. 신경차단술 시행군과 수술을 시행한 군의 치료결과 분석은 Kim's criteria (김의 평가기준), VAS(Visual analog scale) score를 이용하였다. 결과: 연구 대상 환자 69예 중 37예에서는 차단술 후에 증상 호전이 없어 수술을 시행하였고, 32예에서는 신경차단술 만으로 증상호전 되었다. 젊은 사람(60세 미만)에서 보존적 요법보다는 수술을 하는 경향이 많았으며, 이환 기간이 길수록(6개월 이상) 신경근 차단술 보다는 수술하는 경우가 높았다. 차단술 만으로도 호전된 32예의 환자 추시 결과상 다른 치료 없이 증상 호전이 지속되었던 환자는 17예(24.6%)였고 증상이 재발하여 추가 치료를 필요로 한 환자는 15예이었다. 이중 4예는 수술적 치료를 시행하였다. 결론: 수술이 예정된 하지 방사통 환자를 대상으로 수술 준비 기간 동안에 신경근 차단술을 시행하여 그 중 일부에서는 장기 추시에서도 증상 호전이 지속 되었다. 따라서 신경차단술은 수술 전에 한번 시도해 볼 만한 방법 중의 하나로 사료된다. Purpose: To evaluate the effectiveness of a selective nerve root block (SNRB) in patients with sciatica before surgery through a more than 5 year follow up. Materials and Methods: Between January 2000 and December 2000, patients with sciatica, who were recommended to undergo surgery to diagnose and treat sciatica by other clinics or at our hospital, were selected to undergo SNRB before surgery. Among them, 69 patients were followed up for 5 years. The analysis was based on the patients' medical records and telephone interviews, and the treatment results in the SNRB group and operation group were analyzed using Kim's criteria and the Visual Analog Scale score. Results: Among the 69 patients, there was no improvement in symptoms in 37 patients after SNRB. Therefore, they underwent surgery. The symptoms of the remaining 32 patients were improved by SNRB. Overall, younger patients and those with a longer symptom duration required surgery. Conclusion: SNRB was performed on patients with sciatica who were scheduled to undergo surgery. Some patients showed improvement in their symptoms in the long term. Therefore, SNRB should be considered as a treatment option prior to surgery.

Effect of Hydride Reorientation on Delayed Hydride Cracking In Zr-2.5Nb Tubes

Yun Yeo Bum,Kim Young Suk,Im Kyung Soo,Cheong Yong Moo,Kim Sung Soo Korean Nuclear Society 2003 Nuclear Engineering and Technology Vol.35 No.6

The objective of this study is to investigate the reorientation of hydrides with applied stress intensity factor, the peak temperature and the time when to apply the stress intensity factor in a Zr-2.5Nb pressure tube during its thermal cycle treatment. Cantilever beam (CB) specimens with a notch of 0.5 mm in depth made from the Zr-2.5Nb tube were subjected to electrolytic hydrogen charging to contain 60 ppm H and then to a thermal cycle involving heating to the peak temperature of either 310 or $380^{\circ}C$, holding there for 50 h and then cooling to the test temperature of $250^{\circ}C$. The stress intensity factor of either 6.13 or $18.4\;MPa\sqrt{m}$ was applied at the beginning of the thermal cycle, at the end of the hold at the peak temperatures and after cooling to the test temperature, respectively. The reorientation of hydrides in the Zr-2.5Nb tube was enhanced with the increased peak temperature and applied stress intensity factor. Furthermore, when the CB specimens were subjected to $18.4\;MPa\sqrt{m}$ from the beginning of the thermal cycle, the reoriented hydrides occurred almost all over the Zr-2.5Nb tube, surprisingly suppressing the growth of a DHC crack. In contrast, when the CB specimens were subjected to the stress intensity factor at the test temperature, little reorientation of hydrides was observed except the notch region, leading the Zr-2.5Nb to grow a large DHC crack. Based on the correlation between the reorientation of hydrides and the DHC crack growth, a governing factor for DHC is discussed along with the feasibility of the Kim's DHC model.

Physical and chemical Effects on the sonication Treatment of chitosan solution

Lee, Keun Tai,Park, Seong Min,Park, Chan Kyu,Kim, Sang Moo 한국키틴키토산학회 1997 한국키틴키토산학회지 Vol.2 No.2

물리적 방법을 이용한 키토산 올리고당의 제조에 관한 기초 자료를 제시하기 위하여 키토산용액을 20kHz의 초음파로 처리하였으며, 초음파 처리 효과에 미치는 처리 조건의 영향을 조사하였다. 키토산 용액의 고유점도는 초음파 처리 5분까지는 급속하게 감소하였고 그 이후로는 서서히 감소하였다. 키토산 용액의 부피가 작을수록 초음파 처리효과는 컸으며, 키토산 용액의 온도는 초음파 처리에 별다른 효과가 없는 것으로 나타났다. 5분간 초음파 처리 후의 점도는 용매의 종류에 따라 큰 차이가 없었으나, acetate buffer의 경우 초음파 처리효과가 가장 컸다. pH가 높을수록 초음파 처리효과는 크게 나타났으며, 이온강도와 염의 종류에 따른 효과는 나타나지 않았다. 이상의 결과에서, 용액의 부피는 10~20ml, 온도는 20~30℃, 용액의 pH는 4.5, 그리고 용매의 종류는 acetate buffer로 하는 것이 최적 초음파 처리조건으로 추정된다. As the first step of studies related to production of chitooligosaccharides by physical methods, chitosan solution were sonicated with 20kHz and various treatment effects were examined to present fundamental data of sonicated chitosan solution. Intrinsic viscosity of chitosan solution sharply decreased from 3.76dl/g to 2.90dl/g until 5 minutes of sonication and then slowly decreased. With low volume of chitosan solution, sonication was very effective and temperature of chitosan solution slightly affected the efficiency of sonication. In case of changing the solvent, no significant differences were observed on the effect of sonication, however, acetate buffer had highest sonication effect among various solvents. The sonication effect was increased as the increasement of the value of pH, on the contrary, ionic strength and type of counterions showed no effect on sonication, With these results, we assumed that optimal sonication treatment would be as follows, solution volume was 10 ~20ml, temperature range was 20~30℃ , pH value of solution was 4.5 and type of solvent was acetate buffer

PURIFICATION OF PHOSPHOLIPASE C HYDROLYZING PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE FROM CYTOSOLIC PRACTION OF SOYBEAN SPROUTS

Kim, Kee-Hong,Bae, Dong-Soon,Kim, Woe-Yeon,Kim, Jae-Won,Shin, Yong-Chul,Cho, Moo-Je,Lee, Sang-Yeol Gyeongsang National University Chinju, Korea 1992 Plant molecular biology and biotechnology research Vol.1992 No.

Effect of Lyoprotectant on the Solubility and Structure of Silk Sericin

( Moo Kon Kim ),( Hyo Won Kwak ),( Jeong Yun Lee ),( Hae Sung Yun ),( Min Hwa Kim ),( Ki Hoon Lee ) 한국잠사학회 2012 International Journal of Industrial Entomology Vol.25 No.1

To increase the solubility of lyophilized sericin, we added three types of lyoprotectant: sucrose, trehaloseand dextran. The addition of lyoprotectant increased the solubility of lyophilized sericin especially when 1.0% of sucrose, 1.0% and 1.5% trehalose are added. The secondary structure of lyophilized sericin showed that the content of β-sheet or aggregated structure reduced in the presence of lyoprotectant. The morphology of lyophilized was also affected by the addition of lyoprotectant. Whereas flake structure was obtained in the case of pure sericin, a scattered andrelatively small flake structure was formed in the presence of lyoprotectant. These finding shows that the presence of lyoprotectant prevents aggregation of sericin molecules and increases the solubility of lyophilized sericin.

Antioxidant Effect of Leaf, Stem, and Root Extracts of Zingiber officinale as Cosmetic Materials

Sang Moo Lee,Chun Dug Kim 한국피부과학연구원 2021 아시안뷰티화장품학술지 Vol.19 No.1

목적: 최본 연구에서는 생강 잎, 줄기, 뿌리 추출물을 응용하여 화장품 소재로서의 가능성을 평가하고자 한다. 방법: 생강 잎, 줄기, 뿌리 추출물의 화장품 소재로의 가능성을 확인하기 위해 항산화효과를 확인하기 위해 DPPH 라디컬 소거능, 총 폴리페놀 및 플라 보노이드 함량, ABTS 라디칼 소거능, SOD 유사활성을 측정하였다. 그리고 H2O2에 의해 유발되는 산화적 스트레스에 대한 HaCaT 세포 보호효과를 확인하였으며, 마지막으로 모유두 세포 증식효과를 확인하였다. 결과: 첫째, 항산화 효과 실험 결과, DPPH 라디 칼 소거능을 측정한 결과 400 μg/mL의 농도에서 생강 잎 추출물은 88.78%, 줄기 추출물은 70.12%, 뿌리 추출물은 65.52%으로 확 인되었으며, 총 폴리페놀 함량은 생강 잎, 줄기, 뿌리 추출물은 각각 170.22, 120.27, 146 μg/mL으로 확인되었으며, 총 플라보노 이드는 각각 98.52, 70.26, 46.12 μg/mL의 함량이 확인되었다. ABTS radical 소거능을 측정한 결과 400 μg/mL의 농도에서 생강 잎, 줄기, 뿌리 추출물은 각각 88.26, 70.73, 64.13%으로 확인되었으며, SOD 유사활성 측정결과 생강 잎, 줄기, 뿌리 추출물은 각 각65.22, 57.53, 50.21%의 소거능이 확인되었다. 둘째, 과산화수소로 유도된 HaCaT 세포의 산화적 손상에 대한 보호효과는 100 μg/mL 농도에서 생강 잎 추출물은 82.18%, 줄기 추출물은 78.98%, 뿌리 추출물은 70.27%의 생존율이 확인되었다. 실험 결과 생 강 잎 추출물이 32%의 증가율을 나타냄으로써 과산화수소로 유도된 세포 손상에 대해 보호효과가 높게 확인되었다. 셋째, 모유두 세포 증식효과는 생강 잎, 줄기, 뿌리 추출물을 72시간 배양한 결과 100 μg/mL 농도에서 생강 잎 추출물은 158.63%, 줄기 추출물 은 140.41%, 뿌리 추출물은 132.40%까지 세포 증식효과를 확인할 수 있었다. 결론: 이상의 연구 결과 생강 부위별 추출물 중 잎 추 출물이 항산화효과, HaCaT 세포 보호효과, 모유두 세포 증식효과가 가장 우수하여 화장품 소재 개발로 충분한 가능성이 있음을 확 인하였다. Purpose: In this study, we used Zingiber officinale (Z. officinale ) leaf, stem, and root extracts to evaluate their potential as cosmetic materials. Methods: We measured DPPH radical-scavenging activity, total polyphenolic and flavonoid contents, ABTS radical scavenging activity, and SOD-like physiological activity to confirm the antioxidant effect to assess the potential of Z. officinale leaf, stem, and root extracts as cosmetic materials. Furthermore, the protective effect on oxidative stress caused by H2O2 in HaCaT cells and proliferative effect in dermal papilla cells were assessed. Results: As a result of the antioxidant effect of Z. officinale leaf, stem, and root extracts, DPPH radical-scavenging activity was measured at a concentration of 400 μg/mL, and it was 88.78% in Z. officinale leaf extract, 70.12% in stem extract, and 65.52% in root extract. Total polyphenolic content was 170.22 μg/mL (leaf), 120.27 μg/mL (stem), and 146 μg/mL (root), whereas total flavonoid content was 98.52 μg/ mL (leaf), 70.26 μg/mL (stem), and 46.12 μg/mL (root). ABTS radical scavenging activity was measured at a concentration of 400 μg/mL, and it was 88.26% (leaf), 70.73% (stem), and 64.13% (root). Further, SOD-like physiological activity was 65.22% (leaf), 57.53% (stem), and 50.21% (root). The protective effect on oxidative stress caused by H2O2 in HaCaT cells was 82.18% (leaf), 78.98% (stem), and 70.27% (root) at a concentration of 100 μg/mL. Our results confirmed that Z. officinale leaf extract has high protective effects on cell damage caused by H2O2, showing a 32% increase. Moreover, the proliferative effect in dermal papilla cells was 158.63% (leaf), 140.41% (stem), and 132.40% (root) when cells were cultured for 72 h at a concentration of 100 μg/mL. Conclusion: Thus, Z. officinale leaf extract has the highest antioxidant effect, HaCaT cell protective effect, and proliferative effect in dermal papilla cells, indicating a possibility to be developed as a cosmetic material.

Effects of Black Sugar^® and Mineral^® Supplementation on Growth performance and Meat Quality of Hamwoo Steers in Fattening Period

Kim, Kwan Sik,Lee, Sang Moo The Korean Society of Grassland and Forage Science 2014 한국초지조사료학회지 Vol.34 No.3

This study was carried out investigate the effects of dietary addition of mineral and sugar on the dry matter intake, daily gain, yield grade and quality grade of Hanwoo (Bos taurus coreanae) steers. Three diets fed to steers included a control diet (concentrate mix and rice straw : C) and two treatments diet (control diet + black sugar 100 g + mineral 100 g : T1, and control diet + black sugar 150 g + mineral 50 g : T2). The results are summarized as follows; total feeding intake, body weight gain and daily gain did not show significant differences among the three treatments. Cold carcass weight was significantly (p<0.05) higher in T2 than in the other two treatments (C and T1). There was no significant difference in yield traits of back fat thickness, longissimus muscle area and yield grade among the three treatments (C, T1 and T2). Marbling score showed significantly (p<0.05) higher in order of T2 (5.67) > T1 (4.67) > C (3.67). Meat color, fat color, texture and maturity were no significant difference. Quality grade was higher in T2 than in the other two treatments (C and T1), but there was no significant difference. The results show that marbling score and quality grade of Hanwoo can be increased by high dry matter intake with feeding addition of mineral and sugar.

[Poster] Pancreatosplenectomy in left-sided pancreatic cancer : is splenectomy still performed without grounds

Sung Hoon Kim,Ho Kyoung Hwang,Chang Moo Kang,Woo Jung Lee 대한외과학회 2011 대한외과학회 학술대회 초록집 Vol.2011 No.11

ISOLATION AND CHARACTERIZATION OF SOYBEAN PROTEIN KINASE GENES CONTAINING ACIDIC DOMAIN AT THE C-TERMINUS

Kim, Min-Chul,Yoon, Hae-Won,Shin, Pyung-Gyun,Hwang, In-Hwan,Hong, Jong-Chan,Bahk, Jeong-Dong,Lee, Sang-Yeol,Cho, Moo- Je Plant Molecular Biology & Biotechnology Research C 1994 Plant molecular biology and biotechnology research Vol.1994 No.

Four different cDNA clones (SPK-1, SPK-2, SPK-3 and SPK-4) encoding protein serine/threonine kinase were isolated from a soybean (Glycine max L. cv. Williams) cDNA library, and their nucleotide sequences were determined. Both SPK-1 and SPK-2 contained 339 amino acid residues and they were 94% identical at the amino acid sequence level. In contrast, SPK-3 and SPK-4 encoded 351 and 349 amino acid residues, respectively and shared 92% identity in the deduced amino acid sequences. Especilly, SPK-3 and SPK-4 contain stretches of acidic amino acid residues (aspartic acid or glutamic acid) at their carboxy terminal regions. We expressed SPK-2 SPK-4 cDNA in E.coli using a pRSET A vector, and antibody was raised against the expressed recombinant SPK-4 protein in the rabbit. Overexpressed SPK-2 and SPK-4 protein in E.coli showed different phosphorylation patterns. This study showed that cDNA clones isolated from soybean encoded functional protein kinases and their substrates were different. From the Western blot analysis by using SPK-4 polyclonal antibody, we can suppose that the homologous protein kinases to SPK-4 protein containing highly acidic amino acid regions exist in many other plant species and they perform novel regulatory roles in plant cells.