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( Mochamad Yusuf ),( Mochamad Thaha ),( Nilamsari Wenny ),( Mochamad Amin ),( Dokter Pranawa ),( Yasuhiko Tomino ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: A symmetric dimethylarginine (ADMA) is a potent endogenous inhibitor of nitric oxide synthases (NOS) which could reduce nitric oxide (NO) level and causesendothelial dysfunction, vasoconstriction, elevation of blood pressure, and as a potent predictor of cardiovascular event in end-stage kidney disease (ESKD) patients as well. Experimental evidence has convincingly shown that inhibition of NOS by ADMA leads to enhanced norepinephrine release from sympathetic nerves in rats and humans, whereas sympathetic activation impairs endothelium-dependent, nitric-oxide-mediated vasodilatation in healthy humans and ESKD patients. ADMA is formed through the catalytic activity of protein arginine methyltransferases (PRMTs). Thus, type 1 PRMT (PRMT-1) catalyzes the asymmetric dimethylation of arginine residues. Moreover, there are several pathways lead to the increased ADMA level, where PRMT-1 gene polymorphism has been one of the hypothesis. Methods: A total of 56 patients (total sampling) from a private hospital were enrolled in this study. Venous blood samples were taken just before hemodialysis. ADMA was measured using an enzyme-linked immuno- sorbent assay. All samples went through processes of DNA extraction, Polymerase Chain Reaction, and Electrophoresis. Thirteen out of 56 samples (consecutive) were further undergone DNA isolation and purifi cation, labelling, precipitation and sequencing. Genotyping of the polymorphisms was performed using PCR-based SNP detection methods (Applied Bio- systems, Carlsbad, USA)Conclusions: It appears that PRMT-1 gene polymorphism may partially explain the ADMA increasement in hemodialysis patients.
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Muhammad Yusuf Abduh,Mochamad Hirza Nadia,Syaripudin,Robert Manurung,Ramadhani Eka Putra 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.3
A systematic study on the use of Philippine tung (Reutealis trisperma) seed as a substrate for the cultivation of black soldier fly larvae (Hermetia illucens) was performed. The characteristics of Reutealis trisperma seed from two different locations: West Java and Papua, were determined. The seed has a relatively high oil (37.6–39.2%, dry weight) and protein content (14.9–28.2%, dry weight). The effect of cake content in the substrate (0–20%, wet weight), moisture content in the substrate (50–70%, wet weight), feeding rate (50–100 mg/larva/d), lighting condition (dark-light) and substrate depth in a rearing container (4–10 cm) was performed. An optimum prepupal biomass productivity of 123.4 g/m2/d was obtained (20%, wet weight of cake content in the substrate, 60%, wet weight of moisture content in the substrate, 100 mg/larva/d, dark, 6 cm substrate depth). The protein and oil content of the biomass were also determined to evaluate the effect of Reutealis trisperma seed as a substrate for the cultivation of black soldier fly larvae to produce protein and oil-rich biomass. The oil content in the biomass was also extracted and the fatty acid composition was identified. The prepupal biomass has a relatively high amount of protein (45%, dry weight) and oil content (26.6%, dry weight) and is suitable for cattle feed application.
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Potential of White Jack Bean (Canavalia ensiformis L. DC) Kefir as a Microencapsulated Antioxidant
Dandy Yusuf,Risa Kholifaturrohmah,Mochamad Nurcholis,R. Haryo Bimo Setiarto,Lutfi Anggadhania,Sulistian 한국식품영양과학회 2023 Preventive Nutrition and Food Science Vol.28 No.4
Oxidative stress plays a major role in the pathogenesis and progression of noncommunicable diseases. Kefir is a fermented food that has been reported to repress oxidative stress. This study aimed to assess the antioxidant activity, bioactive composition, and encapsulation efficiency of white jack bean (WJB) kefir. The following procedures were conducted: WJB was prepared and converted into juice using water solvent. The sterilized WJB juice was then fermented with kefir grain (10%) for 24∼72 h. Every 24 h, the kefir was evaluated for antioxidant activity, and the dominant bioactive component suspected to be the source of the antioxidant activity was identified. The final stage was the encapsulation process. WJB kefir showed high antioxidant activity, inhibiting DPPH radicals by 90.51±4.73% and ABTS radicals by 86.63±2.34% after 72 h of fermentation. WJB kefir contained 0.35±0.01 mg GAE/g total phenolics and 0.08 mg/g total flavonoids. The LC/MS identification suggested that the bioactive antioxidant components of the WJB kefir were from the alkaloid, saponin, phenolic, and flavonoid groups. The encapsulation with maltodextrin using freeze drying resulted in microencapsulation of WJB kefir with a particle size of 6.42±0.13 m. The encapsulation efficiency was 79.61%, and the IC50 value was 32.62 ppm. The encapsulation method was able to maintain the antioxidant stability of the kefir and extend its shelf life. WJB kefir, a nondairy, lactose-free kefir, can be used as an antioxidant functional food.
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HADIPUTRAWAN, I PUTU WIRA,PUTRA, MAHASENA,IRFAN, MOCHAMAD,YUSUF, MUHAMMAD The Korean Astronomical Society 2015 天文學論叢 Vol.30 No.2
We present the results of visual double stars speckle observations from 2013 using a Zeiss Double Refractor 60 cm with visual focal length f = 1,078 cm, and CCD SBIG ST-402 MEA. A Bessel V filter with ${\lambda}=550nm$ was placed in front of the CCD camera to reduce the chromatic aberration of the objective lens. The objects selected for this observation were calibration candidates and program stars with separations ranging from 0.9-6 arc second, and were located in both the northern and southern hemispheres. Seeing at Bosscha Observatory is generally 1-2 arc second, imposing a limit on visual double star separation below which the system cannot be resolved by long exposure imaging (longer than ~50 ms). Speckle interferometry methods are used to resolve double stars with separations below the typical size of seeing effects. A series of images were captured in fast short-time exposures (~50 ms) using a CCD camera. The result of our experiment shows that our system can be used to measure separations of 0.9 arc second (for systems with small ${\Delta}m$) and ${\Delta}m{\approx}3.7$ (for wide systems).
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