Expression profiles and bioinformatic analysis of microRNAs in myocardium of diabetic cardiomyopathy mice

Wang Ting,Yuan Lingling,Chen Yanxia,Wang Jing,Li Na,Zhou Hong 한국유전학회 2023 Genes & Genomics Vol.45 No.8

Background MicroRNAs (miRNAs) can regulate expression of target genes at post transcriptional level, and mediate the pathophysiological process of many diseases. Objective The study will illuminate the miRNA expression profiles of diabetic cardiomyopathy (DCM), seeking probable biomarkers of DCM at early stage and determining a target for the treatment of DCM. Methods Db/db mice were used as an animal model of type 2 diabetes mellitus. At 22 weeks of age, cardiac function was evaluated by echocardiography, and the structural changes in myocardium were evaluated by HE staining and TEM. The miRNA expression profiles were detected using miRNA sequencing and differentially expressed miRNAs were validated by real-time PCR. Bioinformatic analysis was used to analyze target genes of these miRNAs and relevant pathways in DCM. Results The results showed that 40 miRNAs were differentially expressed, including 28 upregulated miRNAs and 12 downregulated miRNAs. GO and KEGG pathway analysis showed that the target genes of up-regulated miRNAs were involved in 66 pathways, including Wnt, p53 and calcium signaling pathways, as well as FOXO and apoptosis signaling pathways, etc. The target genes of down-regulated miRNAs were involved in 68 pathways, including mitophagy, Ras and MAPK signaling pathways, etc. Moreover, some differentially expressed miRNAs were found in myocardium of DCM for the first time, such as miR-7225-5p, miR-696, miR-3470a, miR-3470b, miR-6240, miR-6538, miR-5128, miR-1195, miR-203-3p and miR-330-5p. Conclusions It is hoped that a few novel molecular pathways or targets of treatment for DCM would be found through understanding the expression features of miRNAs in diabetic myocardium.

AN EFFICIENT METHOD TO SIMULATE JET IN WAVE ENVIROMENT

LINGLING WANG,NA ZHONG,SHENGYI ZHENG 한국수자원학회 2005 한국수자원학회 학술대회 Vol.2005 No.1

Eco-friendly Grafting of Chitosan as a Biopolymer onto Wool Fabrics Using Horseradish Peroxidase

Lingling Xu,Nan Zhang,Qiang Wang,Jiugang Yuan,Yuanyuan Yu,Ping Wang,Xuerong Fan 한국섬유공학회 2019 Fibers and polymers Vol.20 No.2

Chitosan and enzymes have been extensively used in modification of wool, due to their non-toxic and eco-friendly characteristics, as well as to reduce pollution in textile production. In the present work, chitosan was grafted onto wool fabrics using horseradish peroxidase (HRP) in order to endow wool with outstanding properties. The reaction mechanism, grafting percentage, and properties of chitosan-grafted pretreated wool fabric including wettability, dyeability, shrink resistance, and antibacterial activity were studied. P-hydroxyphenylacetamide (PHAD), as a model compound for tyrosine residues in wool,was used to study the mechanism of HRP-mediated grafting of chitosan onto wool. UV and FTIR analyses indicated that quinones, which are intermediate of HRP/H2O2-catalyzed PHAD, reacted with the amino groups of chitosan by Schiff base or Michael addition reactions. Scanning electron microscopy (SEM) showed that dichloroisocyanuric acid pretreatment had an etching effect and some substances were deposited onto the wool fiber. SEM and Fourier transform infrared (FTIR) spectroscopy further confirmed the covalent grafting of chitosan onto wool. Compared with chitosan-grafted unpretreatedwool, there was an obvious improvement in grafting efficiency of chitosan onto wool fabric, physical and mechanical properties after pretreatment and chitosan grafting on the wool. For chitosan-grafted pretreated wool fabrics, there was no observable change in the thermal behavior, while the hydrophilicity, dyeability, shrinkage resistance, and antibacterial activity were remarkably improved compared to that of untreated wool. The present work offers a new ecologically acceptable process of grafting chitosan on the wool.

Effect of Sc Content on the Microstructure and Properties of Al–Mg–Sc Alloys Deposited by Wire Arc Additive Manufacturing

Lingling Ren,Huimin Gu,Wei Wang,Shuai Wang,Chengde Li,Zhenbiao Wang,Yuchun Zhai,Peihua Ma 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.1

Despite showing considerable potential, wire arc additive manufacturing (WAAM) has been limited to producing Al–Mgalloys. As the addition of Sc can improve the mechanical properties of Al–Mg alloys, in this study, Al–Mg–Sc alloy depositswith different Sc contents were prepared by a WAAM process. The porosity, microstructure, and mechanical properties ofthe deposits were studied. At Sc contents less than 0.15%, Sc was completely dissolved in the Al matrix without grain refinement,and the mechanical properties were slightly improved. At a Sc content of 0.3%, the primary Al3Scphase precipitatedout of the as-deposited body, resulting in an abrupt change in the microstructure, significant grain refinement, significantlyincreased tensile strength and yield strength, with a tensile strength, yield strength, and elongation of 372 MPa, 270 MPa,and 22.5%, respectively. Following heat treatment at 350 °C for 1 h, the grain boundaries were refined and the secondaryAl3Scphase was precipitated. Furthermore, the tensile strength and yield strength were significantly increased to 415 MPaand 279 MPa, respectively, and elongation decreased to 18.5%. At a Sc content of 0.45%, due to the aggregated precipitatedphase, the coarsened grain boundaries and the limited solid solution amount of Sc in the α(Al) matrix (~ 0.25%), themechanical properties were not further improved compared with the deposit with Sc content of 0.3%. Thus, based on economicconsiderations, the optimal Sc content was 0.3%. Owing to their enhanced mechanical properties, WAAM-producedAl–Mg–Sc alloys are expected to have a wide range of applications in aviation, aerospace.

大学英语课程中的语篇分析与教学策略研究

王玲玲(Lingling Wang) YIXIN 출판사 2023 教育教学研究论丛 Vol.1 No.3

Optimal type and ratio of foaming agent, foaming stabilizer and fluxing agent of foam glass fabricated with red mud and coal gangue

Jina Wang, Kaidong Xu,Zhixin Li,Yilong Yang,Qingxiao Li,Yun Bao,Huan Yang,Lingling Ding,Ruixin Zhang,Yuanyuan Wang,Lan Yao 한양대학교 세라믹연구소 2022 Journal of Ceramic Processing Research Vol.23 No.1

In order to effectively reuse red mud and coal gangue, foam glass was successfully prepared by sintering. The foaming of foamglass is ensured by adding foaming agent, foam stabilizer and fluxing agent to the raw materials including red mud, coalgangue and waste glass. Furthermore, the defects in the foam glass are also reduced. The obtained powder was dried at 105ºC and characterized by XRF, DSC-TG analysis, etc. According to cellular structure, apparent density and water absorption,the optimal type and ratio of foaming agent, foaming stabilizer and fluxing agent were revealed. The results indicate that theoptimized formula of foaming agent, foaming stabilizer and fluxing agent is 3% MnO2-2% Na3PO4-2% borax. The foam glassprepared under optimized conditions exhibits uniform porous distribution and suitable pore size with an apparent density of0.575 g/cm3 and water absorption of 4.61%. The research in this work could establish the technical foundation of red mudbasedporous material for industrial production.

Transcriptome profiling of indole-3-butyric acid-induced adventitious root formation in softwood cuttings of the Catalpa bungei variety ‘YU-1’ at different developmental stages

Peng Wang,Ya Li,Lingling Ma,Shu’an Wang,Linfang Li,Rutong Yang,Yuzhu Ma,Qing Wang 한국유전학회 2016 Genes & Genomics Vol.38 No.2

Catalpa bungei is a deciduous tree native to China. It is characterized as fast growing, being highly adaptable, and having excellent wood qualities. To better understand potential mechanisms involved in adventitious root (AR) formation, we performed transcriptome analysis of softwood cuttings of C. bungei ‘Yu-1’ at three stages of AR formation using the Illumina sequencing method. Following de novo assembly, 62,955 unigenes were obtained, 31,646 (50.26 %) of which were annotated. A total of 11,100 differentially expressed genes (DEGs), including 10,200 unique and 900 common, were identified in four comparisons. Based on the all GO enrichment networks, 46 common and 7 unique GO categories were identified. Cytoskeleton was only significantly enriched in the activation period, while DNA metabolic process was only significantly enriched in the callus formation. Functional annotation analysis revealed that many of these genes were involved in phenylpropanoid biosynthesis, glycolysis, and plant hormone metabolism, suggesting potential contributions to AR formation. Interestingly, the number of DEGs involved in glycolysis decreased while the number of DEGs involved in phenylpropanoid biosynthesis increased following the AR formative process. Overall, our comprehensive transcriptional overview will prove useful, not only in the understanding of molecular networks that regulate AR formation in C. bungei, but also for exploring genes that may improve rooting rates of other trees.

Transcriptome analysis reveals sunflower cytochrome P450 CYP93A1 responses to high salinity treatment at the seedling stage

Peng Wang,Lingling Ma,Ya Li,Shu’an Wang,Linfang Li,Rutong Yang 한국유전학회 2017 Genes & Genomics Vol.39 No.6

Sunflower (Helianthus annuus) is an important global source of plant lipids and protein for food and other industries. Salinity is an abiotic stress that affects sunflower yield. Although recent studies have revealed that the cytochrome P450 (CYP) 93 family is involved in biotic and abiotic stresses, the relationship between the CYP93A gene and salt stress is not well understood in sunflower. In the present study, we performed transcriptome analysis of a relatively salt-tolerant sunflower variety ‘Xinkui 10’ at the seedling stage under high salinity treatment. Following de novo assembly, 66,426 unigenes were obtained, 45,724 (68.83%) of which were annotated. 5,852 differentially expressed genes (DEGs) were identified 11 distinct clusters in which three main clusters (K1–K3) accounted for 94.39% of DEGs. Mapman analysis showed that CYP450 genes were greatly enriched in cluster K3 and one continuously up-regulated gene, HaCYP93A1, was identified among 248 common DEGs in the transcriptome of ‘Xinkui 10’. Real-time quantitative RT-PCR (qRT-PCR) analysis indicated that transcript levels of HaCYP93A1 were induced by high salinity and jasmonic acid (JA) in roots. Meanwhile, three JA-biosynthesis genes (allene oxide cyclase 3 [AOC3], lipoxygenase 3 [LOX3], and allene oxide synthase [AOS]) in roots were up-regulated under salinity treatment. Moreover, correlation analysis indicated that the expression of HaCYP93A1 was markedly related with the JA-biosynthesis genes. Our results suggested that HaCYP93A1 might be involved in the salt tolerance pathway by regulating JA signaling in sunflower.

Comprehensive transcriptome analysis discovers novel candidate genes related to leaf color in a Lagerstroemia indica yellow leaf mutant

Ya Li,Zhenyu Zhang,Peng Wang,Shu’an Wang,Lingling Ma,Linfang Li,Rutong Yang,Yuzhu Ma,Qing Wang 한국유전학회 2015 Genes & Genomics Vol.37 No.10

Lagerstroemia indica is a popular woody ornamental plant throughout the world. However, relatively little is known about the molecular processes regulating leaf color in L. indica compared with other ornamental plants. Although yellow leaf mutants from various organisms have been well characterized, L. indica yellow leaf mutant has not yet been reported. In this study, a L. indica yellow leaf mutant, named YL03, was characterized and its leaf transcriptome was sequenced. A total of 30,712,752 reads were generated and assembled de novo into 45,308 unigenes with an average length of 987.51 bp. Among these unigenes, 21,339 (47.10 %) were identified as putative homologs of annotated sequences in public databases. A total of 79 unigenes involved in chlorophyll biosynthesis and degradation, photosynthesis and chloroplast development were identified. The expression levels of those genes were detected using quantitative real-time PCR in this study. Among those genes, 11 unigenes showed highly significant difference in the mutant compared to wild type plants. Conclusively, the leaf color formation is greatly affected by the activity of chloroplast development and chlorophyll metabolism. And the possible formation pathway of yellow leaf mutant is deduced based on our results.

Drug Reaction with Eosinophilia and Systemic Symptoms Associated with Reactivation of Epstein-Barr Virus and/or Cytomegalovirus Leading to Hemophagocytic Syndrome in One of Two Patients

( Jianhua Liang ),( Hui Qu ),( Xiaowen Wang ),( Aiping Wang ),( Lingling Liu ),( Ping Tu ),( Ruoyu Li ),( Mingyue Wang ) 대한피부과학회 2018 Annals of Dermatology Vol.30 No.1

Drug reaction with eosinophilia and systemic symptoms (DRESS) is a hypersensitivity reaction characterized by maculopapular rash, exfoliative dermatitis, lymphadenopathy, fever, eosinophilia, and involvement of internal organs. Evidence for reactivation of herpes family viruses has been observed in some DRESS patients, and activated CD8+ T lymphocytes are largely directed against Epstein-Barr virus. Here, we report two cases complicated with this infection. Both patients received antibiotics and non-steroidal anti-inflammatory drugs. These patients manifested clinically with high fever, facial edema, diffuse pruritic erythroderma and maculopapules over the entire body, purpuric rashes in both lower limbs and lymphadenopathy of cervical and inguinal nodes. Laboratory tests revealed abnormal liver function, blood eosinophils, and ferritin levels. The patients recovered completely; however, the female patient developed hemophagocytic syndrome on the 15th day of illness. She developed new itchy rash, and laboratory tests rapidly worsened with fibrinogen levels dramatically reduced to 0.61 g/L. Bone marrow aspiration revealed an increased number of macrophages with hemophagocytosis and a reversed CD4/CD8 ratio of 0.45. These cases suggest that human herpes virus and coagulation function evaluations are necessary in DRESS patients. (Ann Dermatol 30(1) 71∼74, 2018)