论地方政府不正当竞争行为及其法律规制

Ge Fang-lin 제주대학교 법과정책연구원 2022 국제법무 Vol.14 No.2

The unfair competition behavior of local government is a form of government failure in the process of economic and social transformation in China、which has went through the administrative monopoly the fiscal competition and low human rights competition stages. Administrative monopoly refers to the intervention of local governments and their authorized organizations in the market for local interests, and the use of public power to set obstacles or give preferential treatment to foreign enterprises, so that they can obtain a dominant position in the market competition. Fiscal and tax competition refers to the government behavior that local governments attract foreign capital through low tax rate or other effective ways to reduce tax burden. “Low human rights” competition means that local governments often adopt a “low human rights” competition strategy in attracting investment. By reducing the level of human rights protection of residents in their jurisdiction and reducing the social welfare they should enjoy, they form a low-cost bottomland to attract capital inflows. The unfair competition implemented by local governments has eroded he overall interests of the society, reduced the effectiveness of economic intervention power, and reduced the degree of social members’ recognition of it. The reform of fiscal and tax decentralization has made the local government obtain the motive of carrying out improper competition, and the performance appraisal system has made the local government officials form the motive of carrying out improper competition, which is the external cause of the local government’s unfair competition. The external problem of economic law itself is the internal reason of local government unfair competition. Therefore, we must regulate the local government unfair competition act, and construct the competition order between local governments. Firstly, we should improve some basic legal systems, such as, establishing the judicial review system, to distinguish and rectify the local government unfair competition act、maintaining a unified, free and fair market competition order and protecting citizens’ basic rights. And then, we should reform the fiscal and taxation system, and adjust the structure of the interest of the local government, to eliminate the root of the local government unfair competition act. establishing a local tax system with property tax as the mainstay, reforming the value-added tax and consumption tax systems, introducing an environmental tax, and improve the transfer payment system.

Development of a novel peptide microarray for large-scale epitope mapping of food allergens

Lin, Jing,Bardina, Ludmilla,Shreffler, Wayne G.,Andreae, Doerthe A.,Ge, Yongchao,Wang, Julie,Bruni, Francesca M.,Fu, Zhiyan,Han, Youngshin,Sampson, Hugh A. Elsevier 2009 The Journal of allergy and clinical immunology Vol.124 No.2

<P><B>Background</B></P><P>The peptide microarray is a novel assay that facilitates high-throughput screening of peptides with a small quantity of sample.</P><P><B>Objective</B></P><P>We sought to use overlapping peptides of milk allergenic proteins as a model system to establish a reliable and sensitive peptide microarray-based immunoassay for large-scale epitope mapping of food allergens.</P><P><B>Methods</B></P><P>A milk peptide microarray was developed by using commercially synthesized peptides (20-mers, 3 offset) covering the primary sequences of α<SUB>s1</SUB>-casein, α<SUB>s2</SUB>-casein, β-casein, κ-casein, and β-lactoglobulin. Conditions for printing and immunolabeling were optimized using a serum pool of 5 patients with milk allergy. Reproducibility of the milk peptide microarray was evaluated using replicate arrays immunolabeled with the serum pool, whereas specificity and sensitivity were assessed by using serial dilution of the serum pool and a peptide inhibition assay.</P><P><B>Results</B></P><P>Our results show that epitopes identified by the peptide microarray were mostly consistent with those identified previously by SPOT membrane technology, but with specific binding to a few newly identified epitopes of milk allergens. Data from replicate arrays were reproducible (<I>r</I> ≥ 0.92) regardless of printing lots, immunolabeling, and serum pool batches. Using the serially diluted serum pool, we confirmed that IgE antibody binding detected in the array was specific. Peptide inhibition of IgE binding to the same peptide and overlapping peptides further confirmed the specificity of the array.</P><P><B>Conclusion</B></P><P>A reliable peptide microarray was established for large-scale IgE epitope mapping of milk allergens, and this robust technology could be applied for epitope mapping of other food allergens.</P>

Heterologous Expression and Characterization of a Thermostable α-L-Rhamnosidase from Thermoclostridium stercorarium subsp. thermolacticum DSM 2910 and Its Application in

Ge Lin,Liu Yingying,Zhou Fangming,Zhan Lingling,Zhao Linguo 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.11

An α-L-rhamnosidase gene from Thermoclostridium. stercorarium subsp. thermolacticum DSM 2910 (TstRhaA) was cloned and expressed. The maximum TstRhaA activity of the protein reached 25.2 U/ml, and the molecular mass was approximately 106.6 kDa. The protein was purified 8.0-fold by Ni-TED affinity with an overall recovery of 16.6% and a specific activity of 187.9 U/mg. TstRhaA activity was the highest at 65°C and pH 6.5. In addition, it exhibited excellent thermal stability, better pH stability, good tolerance to low concentrations of organic reagents, and high catalytic activity for pnitrophenyl-α-L-rhamnopyranoside (pNPR). Substrate specificity studies showed that TstRhaA exhibited a high specific activity for rutin. At 60°C, pH 6.5, and 0.3 U/ml enzyme dosage, 60 g/l rutin was converted to 45.55 g/l isoquercitrin within 150 min. The molar conversion rate of rutin and the yield of isoquercitrin were 99.8% and 12.22 g/l/h, respectively. The results suggested that TstRhaA could be used for mass production of isoquercitrin.

On the Infinity of Thoughts of Folding: The Case of Chen Chieh-Jen’s Realm of Reverberations

Ge-Sheng LIN 한국비평이론학회 2016 한국비평이론학회 학술대회 발표집 Vol.2016 No.봄

Penicilazaphilone C, a new antineoplastic and antibacterial azaphilone from the Marine Fungus Penicillium sclerotiorum

Song-lin Zhou,Min Wang,Huan-ge Zhao,Yong-hao Huang,Ying-ying Lin,Guang-hong Tan,Shung-lin Chen 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.12

Two azaphilonidal derivatives [penicilazaphilonesB (1) and C (2)], have been isolated from thefermented products of marine fungus strain Penicilliumsclerotiorum M-22, penicilazaphilones C was a new compound. The compound’s structures were identified by theanalysis of spectroscopic data including 1D and 2D NMRtechniques (1H-NMR, 13C-NMR, COSY, HMQC, andHMBC). Biological evaluation revealed that penicilazaphilonesB and C showed selective cytotoxicity againstmelanoma cells B-16 and human gastric cancer cells SGC-7901 with IC50 values of 0.291, 0.449 and 0.065,0.720 mM, respectively, while exhibiting no significanttoxicity to normal mammary epithelial cells M10 at thesame concentration. Moreover, penicilazaphilones C alsoexhibited strong antibacterial activity against Staphylococcusaureus, Pseudomonas aeruginosa, Klebsiellapneumonia and Escherichia coli with MIC values0.037–0.150 mM, while penicilazaphilones B’s bacteriostaticaction was weaker.

rs10505474 and rs7837328 at 8q24 Cumulatively Confer Risk of Prostate Cancer in Northern Han Chinese

Zhang, Lin-Lin,Sun, Liang,Zhu, Xiao-Quan,Xu, Yong,Yang, Kuo,Yang, Fan,Yang, Yi-Ge,Chen, Guo-Qiang,Fu, Ji-Cheng,Zheng, Chen-Guang,Li, Ying,Mu, Xiao-Qiu,Shi, Xiao-Hong,Zhao, Fan,Wang, Fei,Yang, Ze,Wang, Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Aims: Genome-wide association studies (GWAS) have identified several risk variants for prostate cancer (pCa) mainly in Europeans, which need to be further verified in other racial groups. We selected six previously identified variants as candidates and to define the association with PCa in Northern Han Chinese. Methods: 749 subjects from Beijing and Tianjin in Northern China were included. Six variants (rs10505474, rs7837328, rs4242384, rs7813, rs486907 and rs1058205) were genotyped by high resolution melting (HRM) assays. The individual and cumulative contribution for of the risk of PCa and clinical covariates were analyzed. Results: Among the six candidate variants, onlyrs10505474, and rs7837328, both locating at 8q24 region, were associated with PCa in our population.rs10505474 (A) was associated with PCa ($OR_{recessive}=1.56$, p=0.006); and rs7837328 (A) was associated with PCa ($OR_{dominant}=1.38$, p=0.042/$OR_{recessive}=1.99$, p=0.003). Moreover, we observed a cumulative effects between them ($p_{trend}=2.58{\times}10^{-5}$). The joint population attributable risk showed the two variants might account for 71.85% of PCa risk. In addition, we found the homozygotes of rs10505474 (A) and rs7837328 (A) were associated with PCa clinical covariants (age at onset, tumor stage, respectively) ($p_{age}=0.046$, $P_{tumorstage}=0.048$). Conclusion: rs10505474 (A) and rs7387328 (A) at 8q24 are associated with PCa and cumulatively confer risk, suggesting the two variations could determine susceptibility to PCa in the Northern Chinese Han population.

Identification and Mapping of a Thermo-Sensitive Genic Self-Incompatibility Gene in Maize

Xin Ge Lin,Hui Ling Xie,Zhang Ying Xi,Yan Min Hu,Guang Yuan Zhao,Liu Jing Duan,Zong You Hao,Zong Hua Liu,Ji Hua Tang 한국유전학회 2009 Genes & Genomics Vol.31 No.3

In this study, we describe a novel ecological self-incompatibility (SI) line HE97 in maize. The main environmental factors influencing the inbred line characteristics were identified through field sowing trials during a two-year study period (2001 and 2002). The results showed that daily minimum temperature had the greatest effect on floral morphology and breeding system of the SI line. In staminate floret differentiation, when the daily minimum temperature exceeded 24℃, the line exhibited complete self-compatibility; however SI was observed when the daily minimum temperature was below 20℃. Therefore, we characterized the line as exhibiting thermo-sensitive genic self-incompatibility (TGSI). A set of F2 and F2:3 populations, derived from the inbred lines HE97 and Z58, were evaluated for two years to elucidate the TGSI line patterns of inheritance. Classical genetic analyses and QTL mapping results revealed that HE97 self-incompatibility was governed by a single allele, named here as tgsi1. The tgsi1 gene was mapped to chromosome 2 between SSR markers nc131 and bnlg1633, with a distance of 2.40 cM from nc131 and 2.44 cM from bnlg1633.

Toxicarioside N induces apoptosis in human gastric cancer SGC- 7901 cell by activating the p38MAPK pathway

Huan-ge Zhao,Song-lin Zhou,Ying-ying Lin,Hao Fu Dai,Feng-Ying Huang 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.1

Natural plant compounds with potent proliferationinhibition and apoptosis induction properties havebeen screened as novel anticancer drugs. Toxicarioside N(Tox N) was isolated from the seeds of the tropical plantAntiaris toxicaria in Hainan province, China. To ourknowledge, the effects that Tox N has on the apoptosis ofSGC-7901 cells and its potential mechanism have neverbeen investigated. In this study, we detected the anticanceractivities of Tox N and explored the potential mechanismin the human gastrointestinal cancer cell line SGC-7901. Here, we found that Tox N inhibited SGC-7901 cell growthin a dose- and time-dependent manner and induced apoptosisin cells based on cell morphology and flow cytometryanalyses. Additionally, the SGC-7901 cell treated with ToxN up-regulated the expression level of cleaved caspase-3/9and PARP, increased the Bax/Bcl-2 ratio, and led to therelease of cytochrome c into the cytoplasm. In addition,Tox N treatment led to the phosphorylation of p38MAPK. SB203580, a p38MAPK inhibitor, partially attenuated ToxN induced apoptosis by preventing the activation of caspase-3/9 and PARP. Our results indicated for the first timethat Tox N can induce SGC-7901 cells apoptosis by activatingthe p38MAPK pathway.

Autophagy plays a protective role against apoptosis induced by toxicarioside N via the Akt/mTOR pathway in human gastric cancer SGC-7901 cells

Huan-ge Zhao,Song-lin Zhou,Ying-ying Lin,Hua Wang,Hao Fu Dai,Feng-Ying Huang 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.10

Toxicarioside N (Tox N), a natural product extract from Antiaris toxicaria, has been reported to induce apoptosis in human gastric cancer cells. However, the mechanism and actual role of autophagy in Tox N-induced apoptosis of human gastric cancer cells remains poorly understood. In the current study, we demonstrated that Tox N could induce autophagy by inhibiting the Akt/mTOR signaling pathway in SGC-7901 cells. Moreover, we found that the inhibition of autophagy by 3-methyladenine, an autophagy inhibitor, enhanced Tox N-induced apoptotic cell death. However, the stimulation of autophagy by rapamycin, an autophagy activator, remarkably suppressed Tox N-induced apoptosis, suggesting that autophagy plays a protective role in Tox N-induced apoptosis. Thus, the results from this study suggested that Tox N combination with an autophagy inhibitor might be a promising strategy to enhance the anticancer activity of Tox N for the treatment of human gastric cancer.

Aerodynamic and aero-elastic performances of super-large cooling towers

Lin Zhao,Xu Chen,Shitang Ke,Yaojun Ge 한국풍공학회 2014 Wind and Structures, An International Journal (WAS Vol.19 No.4

Hyperbolic thin-shell cooling towers have complicated vibration modes, and are very sensitive to the effects of group towers and wind-induced vibrations. Traditional aero-elastic models of cooling towers are usually designed based on the method of stiffness simulation by continuous medium thin shell materials. However, the method has some shortages in actual engineering applications, so the so-called “equivalent beam-net design method” of aero-elastic models of cooling towers is proposed in the paper and an aero-elastic model with a proportion of 1: 200 based on the method above with integrated pressure measurements and vibration measurements has been designed and carried out in TJ-3 wind tunnel of Tongji university. According to the wind tunnel test, this paper discusses the impacts of self-excited force effect on the surface wind pressure of a large-scale cooling tower and the results show that the impact of self-excited force on the distribution characteristics of average surface wind pressure is very small, but the impact on the form of distribution and numerical value of fluctuating wind pressure is relatively large. Combing with the Complete Quadratic Combination method (hereafter referred to as CQC method), the paper further studies the numerical sizes and distribution characteristics of background components, resonant components, cross-term components and total fluctuating wind-induced vibration responses of some typical nodes which indicate that the resonance response is dominant in the fluctuating wind-induced vibration response and cross-term components are not negligible for wind-induced vibration responses of super-large cooling towers.