QTL Analysis of Floral Traits of Rice (Oryza Sativa L.) under Well-Watered and Drought Stress Conditions

Song Ping Hu,Ying Zhou,Lin Zhang,Xu Dong Zhu,Zheng Gong Wang,Lin Li,Li Jun Luo,Qing Ming Zhou 한국유전학회 2009 Genes & Genomics Vol.31 No.2

Three floral traits, spikelet number per panicle (SNP), percentage of single exserted stigma (PSES) and dual exserted stigma (PDES) of a RI population with 185 lines under water stress and non-stress conditions for two years were investigated in a drought tolerance screening facility. ANOVA results showed high significance between years, lines, and water stress treatments, together with interactions among them in pairs. High phenotypic correlation was found between PSES and PDES (r=0.5424***). Based on a linkage map of 203 SSR markers, when under well-watered condition, six QTLs (qSNP-3b, qSNP-4, qSNP-11 qSNP-2, qSNP-5 and qSNP-9) were detected for SNP. Half of them had significant Q×E interactions. Three QTLs (qPSES-1, qPSES-2, qPSES-5) were found to influence PSES, including one locus (qPSES-2) having Q×E interaction. And three QTLs (qPDES-2, qPDES-5 and qPDES-8) were also detected to influence PDES. qPDES-5 was found to have Q×E interaction. The contribution rate of a single QTL varied from 0.80% to 8.83% for additive effect, and 1.86% to 15.25% for Q×E interactions. Under drought stress, six QTLs (qSNP-3a, qSNP-4, qSNP-7a, qSNP-7b, qSNP-8 and qSNP-9) were associated with SNP, including qSNP-3a and qSNP-4 with Q×E interaction. Three QTLs (qPSES-1, qPSES-10 and qPSES-12) were located on rice chromosome 1, 10 and 12 for PSES. Four QTLs (qPDES-1a, qPDES-1b, qPDES-4 and qPDES-9) were detected for PDES, including qPDES-9 with Q×E interaction. The additive effect of single QTL can only explain 1.16% to 5.84% of total variance while Q×E interaction of four loci can explain 4.25% to 11.54% of total variance for each locus. There were one to nine pairs of epistatic QTLs influencing SNP and stigma exsertion. The contribution rates of additive and epistatic effects seemed to be in a low magnitude for most cases (0.76%~9.92%) while a few QTLs or QTL pairs explained more than 10% of total variance. Some main effect QTL and epistasis were commonly detected among PSES and PDES, explaining the high positive correlation between them. Few QTLs were detected under both water stress and non-stress conditions, indicating that drought had severe impact on the genetic behaviors of both spikelet number and stigma exsertion.

Penicilazaphilone C, a new antineoplastic and antibacterial azaphilone from the Marine Fungus Penicillium sclerotiorum

Song-lin Zhou,Min Wang,Huan-ge Zhao,Yong-hao Huang,Ying-ying Lin,Guang-hong Tan,Shung-lin Chen 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.12

Two azaphilonidal derivatives [penicilazaphilonesB (1) and C (2)], have been isolated from thefermented products of marine fungus strain Penicilliumsclerotiorum M-22, penicilazaphilones C was a new compound. The compound’s structures were identified by theanalysis of spectroscopic data including 1D and 2D NMRtechniques (1H-NMR, 13C-NMR, COSY, HMQC, andHMBC). Biological evaluation revealed that penicilazaphilonesB and C showed selective cytotoxicity againstmelanoma cells B-16 and human gastric cancer cells SGC-7901 with IC50 values of 0.291, 0.449 and 0.065,0.720 mM, respectively, while exhibiting no significanttoxicity to normal mammary epithelial cells M10 at thesame concentration. Moreover, penicilazaphilones C alsoexhibited strong antibacterial activity against Staphylococcusaureus, Pseudomonas aeruginosa, Klebsiellapneumonia and Escherichia coli with MIC values0.037–0.150 mM, while penicilazaphilones B’s bacteriostaticaction was weaker.

Effect of Heat Treatments on Magnetic Properties and Thermal Expansion Behavior in Two Distinct Types of Fe-Ni Invar Alloys

Lin Huang,Tingwen Guo,Yongjian Zhou,Dong Han,Yu Gu,Cheng Song,Feng Pan 한국자기학회 2022 Journal of Magnetics Vol.27 No.4

The casting process of Fe-Ni Invar alloy could drastically affect magnetic properties and thermal expansion. We have observed analogous trends of saturation magnetization and thermal expansion coefficient in two distinct types of casted samples, as we perform post-annealing at different temperatures. X-ray diffraction measurements show that all the alloys remain in the same face-centered cubic structure after different heat treatments without phase transformation. With the help of X-ray diffraction and differential thermal analysis, we have inferred that these changes in properties might be induced by magnetic lattice transitions and analyzed the possible reason for property differences in samples. This work delivers a perspective on the relationships between thermal expansion, magnetic properties, and heat treatments, which could help to improve the industrial assembly line design.

The Relationship between HIF-2α and VEGF with Radiographic Severity in the Primary Osteoarthritic Knee

Zhou Jian-lin,Fang Hong-song,Peng Hao,Deng Shuang,Chen Shen,Li Jian-ping,Qiu Bo,Weng Jin-qing,Liu Feng 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.3

Purpose: The aim of this study was to determine the relationship of hypoxia-inducible factor-2 (HIF-2α) and vascular endothelial growth factor (VEGF) with radiographic severity in primary osteoarthritis (OA) of the knee. Expression of these two factors in cartilagesamples from OA knee joints was examined at mRNA and protein levels. Materials and Methods: Knee joints were examined using plain radiographs, and OA severity was assessed using the Kellgren and Lawrence (KL) grading system. Specimens were collected from 29 patients (31 knees) who underwent total knee replacementbecause of severe medial OA of the knee (KL grades 3 and 4), 16 patients who underwent knee arthroscopy (KL grade 2), and 5 patients with traumatic knees (KL grade 0). HIF-2α and VEGF expression was quantified by real-time polymerase chain reactionand western blotting. Results: Cartilage degeneration correlated with the radiographic severity grade. OA severity, determined using the Mankin scale, correlated positively with the KL grade (r=0.8790, p<0.01), and HIF-2α and VEGF levels with the radiographic severity of knee OA (r=0.7001, p<0.05; r=0.6647, p<0.05). Conclusion: In OA cartilage, HIF-2α and VEGF mRNA and protein levels were significantly and positively correlated. The expressionof both factors correlated positively with the KL grade. HIF-2α and VEGF, therefore, may serve as biochemical markers as well as potential therapeutic targets in knee OA.

A glass fibre membrane platform for ultra-sensitive detection of cardiac troponin T

Song, Keum-Soo,Nimse, Satish Balasaheb,Sonawane, Mukesh Digambar,Lin, Yahui,Zhou,Kim, Taisun The Royal Society of Chemistry 2017 The Analyst Vol.142 No.20

<P>A glass fibre membrane platform that allows quantification of circulating cTnT with a LoD of 0.87 pg mL<SUP>−1</SUP> is described. The proposed platform uses a glass fibre membrane, DNA-guided detection method, and antibody-conjugated fluorescent beads for the quantification of cTnT in the analytical detection range of 1-120 pg mL<SUP>−1</SUP> at room temperature in 30 min. Glass fibre membranes were chemically modified to immobilize the oligonucleotide probes that catch a biomolecular complex (FB-dAB-cTnT-cAB-DNA) containing complementary oligonucleotides. There were no interferences from human cTnI, cTnC, skTnT, biotin, and hemoglobin (each 1 μg mL<SUP>−1</SUP>). The linearity in the serial dilution test of plasma samples indicates that this platform is highly applicable for regular health check-up to assess the risk of AMI and HF.</P>

Prognostic Values of Various Clinical Factors and Genetic Subtypes for Diffuse Large B-cell lymphoma Patients: A Retrospective Analysis of 227 Cases

Zhou, De,Xie, Wan-Zhuo,Hu, Ke-Yue,Huang, Wei-Jia,Wei, Guo-Qing,He, Jing-Song,Shi, Ji-Min,Luo, Yi,Li, Li,Zhu, Jing-Jing,Zhang, Jie,Lin, Mao-Fang,Ye, Xiu-Jin,Cai, Zhen,Huang, He Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Aim: To analyze the significance of different clinical factors for prognostic prediction in diffuse large B-cell lymphoma (DLBCL) patients. Methods: Two hundred and twenty-seven DLBCL patients were retrospectively reviewed. Patients were managed with cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) regimen or rituximab plus the CHOP (RCHOP) regimen. Results: Lactate dehydrogenase (LDH), ${\beta}2$-microglobulin (${\beta}2$-M), B symptoms, Ann Arbor stage and genetic subtypes were statistically relevant in predicting the prognosis of the overall survival (OS). In the CHOP group, the OS in patients with germinal center B-cell-like (GCB)(76.2%) was significantly higher than that of the non-GCB group (51.9%, P=0.032). With RCHOP management, there was no statistical difference in OS between the GCB (88.4%) and non-GCB groups (81.9%, P=0.288). Conclusion: Elevated LDH and ${\beta}2$-M levels, positive B symptoms, Ann Arbor stage III/IV, and primary nodal lymphoma indicate an unfavorable prognosis of DLBCL patients. Patients with GCB-like DLBCL have a better prognosis than those with non-GCB when treated with the CHOP regimen. The RCHOP treatment with the addition of rituximab can improve the prognosis of patients with DLBCL.

SmGDB: genome database of Salvia miltiorrhiza, an important TCM Plant

Zhou Changhao,Lin Caicai,Xing Piyi,Li Xingfeng,Song Zhenqiao 한국유전학회 2022 Genes & Genomics Vol.44 No.6

Background: Salvia miltiorrhiza is an important traditional Chinese medicinal (TCM) plant and a model plant in the genetic study of TCM. A series of omics related to Danshen have been published. Integrating, managing, storing, and sharing data has become an urgent problem to be solved in S. miltiorrhiza genetic studies. Objectives: The genome database is the link for the exchange, acquisition, and use of different omics data between data producers and users, maximizing value and utilization of data. Methods: The genome database included DSS3 genome and five RNA-Seq data. The back-end performs data search and retrieval through the LAMP (Linux, Apache, MySQL, PHP) framework. Results: Here, we present SmGDB (S. miltiorrhiza genome database; http://8.140.162.85/ ), which houses the latest version of genome sequence and annotation data for S. miltiorrhiza, combining three unpublished RNA-Seq data from our group and two released RNA-Seq data. We also identified a novel gene cluster including seven CYP71D genes involved in the tanshinone synthesis pathway based on genome sequences and expression data. Besides, SmGDB provides user-friendly web interfaces for querying and browsing gene annotation, structure, location, and expression profiles for concerned genes. Popular bioinformatics tools such as 'BLAST', 'Search', 'Heatmap', 'JBrowse', etc., were also provided in SmGDB. Conclusions: SmGDB will provide utility for characterizing the structure of the S. miltiorrhiza genome and better understanding gene functions and biological processes underlying complex secondary metabolism in Danshen.

Comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non‑heading Chinese cabbage (Brassica rapa ssp. chinensis) line WS24‑3A

Liping Song,Xia Li,Feng Zu,Changbin Gao,Bincai Wang,Chufa Lin,Jinxing Tu,Aihua Wang,Guolin Zhou 한국유전학회 2019 Genes & Genomics Vol.41 No.12

Background WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. Objective To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. Methods Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. Results Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. Conclusion Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage.

Service Scheduling in Cloud Computing based on Queuing Game Model

( Fuhong Lin ),( Xianwei Zhou ),( Daochao Huang ),( Wei Song ),( Dongsheng Han ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.5

Cloud Computing allows application providers seamlessly scaling their services and enables users scaling their usage according to their needs. In this paper, using queuing game model, we present service scheduling schemes which are used in software as a service (SaaS). The object is maximizing the Cloud Computing platform`s (CCP`s) payoff via controlling the service requests whether to join or balk, and controlling the value of CCP`s admission fee. Firstly, we treat the CCP as one virtual machine (VM) and analyze the optimal queue length with a fixed admission fee distribution. If the position number of a new service request is bigger than the optimal queue length, it balks. Otherwise, it joins in. Under this scheme, the CCP`s payoff can be maximized. Secondly, we extend this achievement to the multiple VMs situation. A big difference between single VM and multiple VMs is that the latter one needs to decide which VM the service requests turn to for service. We use a corresponding algorithm solve it. Simulation results demonstrate the good performance of our schemes.

Alum sludge conditioning with ferrous iron/peroxymonosulfate oxidation: Characterization and mechanism

Xu Zhou,Wenbiao Jin,Lan Wang,Lin Che,Chuan Chen,Shao-feng Li,Xue-Ting Wang,Renjie Tu,Song-fang Han,Xiaochi Feng,Nan-Qi Ren 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.4

Alum sludge produced by drinking water plants needs to be conditioned and dewatered before final disposal. In this study, a novel ferrous iron/peroxymonosulfate (PMS) oxidation process was employed to enhance alum sludge dewaterability. The effect of oxidative sulfate radicals generated by Fe2+ activated HSO5  on alum sludge was studied. The results showed that the optimal conditioning conditions for addition of Fe2+ and PMS were 0.5 g/g and 0.1 g/g TSS, respectively. Meanwhile, the capillary suction time (CST) and specific resistance to filtration (SRF) of alum sludge was reduced by 66% and 88%. Also found was that the absolute value of the zeta potential increased and the particle size decreased in alum sludge after Fe2+-PMS conditioning, which indicated that oxidative sulfate radicals destroyed the floc structure of alum sludge and smaller particles were generated. At the same time, the water contained in sludge flocs was released and enhanced sludge dewaterability, while leaching of aluminum ions also characterized decomposition of alum sludge.