디자인 사례를 통해서 본 지역 문화 콘텐츠의 시각화 방법 연구 -한・중 관광 스탬프를 대상으로-

장징원(Jingyun. Zhang),이연우,안병진 한국멀티미디어학회 2023 멀티미디어학회논문지 Vol.26 No.1

Regional culture generally refers to the unique cultural tradition of a specific region, and is an expression of civilization such as ecology, folklore, tradition, and habits of a specific region. It is a mental and cultural asset unique to the region created by the combination of local culture and environment. Therefore, regional cultural development can not only increase the cultural influence of the region but also bring economic effects. The delivery of local culture can include several aspects of research value and can be done in various ways. However, among them, a method of extracting and visualizing the characteristics of local culture is widely used at this stage. Therefore, among the difficult ways to deepen the mental assets of local culture, it is necessary to study the transmission of local information in a visual manner. The tourism stamp itself shows the great achievements of visualization of local culture. The tourism seal is a kind of architecture, landscape or famous works aimed at the area. It strengthens the connection with the public through the connection with the environment and with the production and activities. Therefore, in order to convey local culture well, this paper used the tourism stamps of four cities in both Korea and China as the starting point of the study and summarized the method of visualizing culture through example research. Five visualization methods have been obtained through the study, and we intend to provide new ideas and methods for cultural visualization in each region in the future.

Assessment of the Cytotoxic and Apoptotic Effects of Chaetominine in a Human Leukemia Cell Line

Yao, Jingyun,Jiao, Ruihua,Liu, Changqing,Zhang, Yupeng,Yu, Wanguo,Lu, Yanhua,Tan, Renxiang The Korean Society of Applied Pharmacology 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.2

Chaetominine is a quinazoline alkaloid originating from the endophytic fungus Aspergillus fumigatus CY018. In this study, we showed evidence that chaetominine has cytotoxic and apoptotic effects on human leukemia K562 cells and investigated the pathway involved in chaetominine-induced apoptosis in detail. Chaetominine inhibited K562 cell growth, with an $IC_{50}$ value of 35 nM, but showed little inhibitory effect on the growth of human peripheral blood mononuclear cells. The high apoptosis rates, morphological apoptotic features, and DNA fragmentation caused by chaetominine indicated that the cytotoxicity was partially caused by its pro-apoptotic effect. Under chaetominine treatment, the Bax/Bcl-2 ratio was upregulated (from 0.3 to 8), which was followed by a decrease in mitochondrial membrane potential, release of cytochrome c from mitochondria into the cytosol, and stimulation of Apaf-1. Furthermore, activation of caspase-9 and caspase-3, which are the main executers of the apoptotic process, was observed. These results demonstrated that chaetominine induced cell apoptosis via the mitochondrial pathway. Chaetominine inhibited K562 cell growth and induced apoptotic cell death through the intrinsic pathway, which suggests that chaetominine might be a promising therapeutic for leukemia.

Arachidonic Acid 15-Lipoxygenase: Effects of Its Expression, Metabolites, and Genetic and Epigenetic Variations on Airway Inflammation

Xu Xu,Li Jingyun,Zhang Yuan,Zhang Luo 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.5

Arachidonic acid 15-lipoxygenase (ALOX15) is an enzyme that can oxidize polyunsaturated fatty acids. ALOX15 is strongly expressed in airway epithelial cells, where it catalyzes the conversion of arachidonic acid to 15-hydroxyeicosatetraenoic acid (15-HETE) involved in various airway inflammatory diseases. Interleukin (IL)-4 and IL-13 induce ALOX15 expression by activating Jak2 and Tyk2 kinases as well as signal transducers and activators of transcription (STATs) 1/3/5/6. ALOX15 up-regulation and subsequent association with phosphatidylethanolamine-binding protein 1 (PEBP1) activate the mitogen-activated extracellular signal-regulated kinase (MEK)-extracellular signal-regulated kinase (ERK) pathway, thus inducing eosinophil-mediated airway inflammation. In addition, ALOX15 plays a significant role in promoting the migration of immune cells, such as immature dendritic cells, activated T cells, and mast cells, and airway remodeling, including goblet cell differentiation. Genome-wide association studies have revealed multiple ALOX15 variants and their significant correlation with the risk of developing airway diseases. The epigenetic modifications of the ALOX15 gene, such as DNA methylation and histone modifications, have been shown to closely relate with airway inflammation. This review summarizes the role of ALOX15 in different phenotypes of asthma, chronic obstructive pulmonary disease, chronic rhinosinusitis, aspirin-exacerbated respiratory disease, and nasal polyps, suggesting new treatment strategies for these airway inflammatory diseases with complex etiology and poor treatment response.

Molecular characterization and phylogenetic analysis of pseudorabies virus variants isolated from Guangdong province of southern China during 2013–2014

Jindai Fan,Xiduo Zeng,Guanqun Zhang,Qiwen Wu,Jianqiang Niu,Baoli Sun,Qingmei Xie,Jingyun Ma 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.3

Outbreaks of pseudorabies (PR) have occurred in southern China since late 2011, resulting in significant economic impacts on the swine industry. To identify the cause of PR outbreaks, especially among vaccinated pigs, 11 pseudorabies virus (PRV) field strains were isolated from Guangdong province during 2013–2014. Their major viral genes (gE, TK, gI, PK, gD, 11K, and 28K) were analyzed in this study. Insertions or deletions were observed in gD, gE, gI and PK genes compared with other PRV isolates from all over the world. Furthermore, sequence alignment showed that insertions in gD and gE were unique molecular characteristics of the new prevalent PRV strains in China. Phylogenetic analysis showed that our isolates were clustered in an independent branch together with other strains isolated from China in recent years, and that they showed a closer genetic relationship with earlier isolates from Asia. Our results suggest that these isolates are novel PRV variants with unique molecular signatures.

Genetic diversity and phylogenetic analysis of porcine reproductive and respiratory syndrome virus in southern China from 2007 to 2014

Qiwen Wu,Zhili Li,Guanqun Zhang,Jianqiang Niu,Xiduo Zeng,Baoli Sun,Jingyun Ma 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.3

Porcine reproductive and respiratory syndrome virus (PRRSV) has a high degree of genetic variation. In this study, we characterized the genetic variation and evolutionary relationships among circulating PRRSV strains in southern China. We analyzed 29 NSP2 strains and 150 ORF5 strains from clinical samples collected in southern China during 2007–2014. The alignment results showed that the nucleotide identity similarities of the two genes among these strains were 80.5%–99.7% and 80.9%–100%, respectively. Phylogenetic analysis based on the NSP2 gene showed that highly pathogenic (HP)-PRRSV was still the dominant virus in southern China from 2013 to 2014. Compared with reference strains CH-1a and VR-2332, the field strain 131101-GD-SHC, which shared high homology with JXA1-P170, had a novel 12 amino acid deletion at position 499-510. Phylogenetic analysis based on the ORF5 gene showed that HP-PRRSV, VR2332-like strains, and QYYZ-like strains were simultaneously circulating in southern China from 2007 to 2014, suggesting that, in recent years, the type 2 PRRSV was more diverse in southern China. In conclusion, mutations in the decoy epitope and primary neutralizing epitope could be markers of viral evolution and used to study evolutionary relationships among PRRSV strains in China.

Sequence analysis of the spike gene of Porcine epidemic diarrhea virus isolated from South China during 2011–2015

Xiaoya Zhao,Zhili Li,Xiduo Zeng,Guanqun Zhang,Jianqiang Niu,Baoli Sun,Jingyun Ma 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.2

The spike gene of porcine epidemic diarrhea virus (PEDV) was sequenced from 55 South China field strains isolated from pigs with symptoms of diarrhea. The sequences were compared within the set of field strains as well as with reference strains available in GenBank. Within the 55 South China PEDV field strains, the deduced amino acid sequence identities ranged from 93.8% to 99.9 % and ranged from 90.7% to 99.5% when compared with the foreign reference strains in GenBank. Our phylogenetic analysis showed that 10 of the 55 South China PEDV strains belonged to G1b and 45 belonged to G2b.

Genetic Analysis and Serological Detection of Novel O-Antigen Gene Clusters of Plesiomonas shigelloides

( Xiaochen Wang ),( Daoyi Xi ),( Yuehua Li ),( Junxiang Yan ),( Jingyun Zhang ),( Xi Guo ),( Boyang Cao ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.4

Plesiomonas shigelloides, a member of the family Vibrionaceae, is a gram-negative, rod-shaped, facultative anaerobic bacterium with flagella. P. shigelloides has been isolated from such sources as freshwater, surface water, and many wild and domestic animals. P. shigelloides contains 102 Oantigens and 51 H-antigens. The diversity of O-antigen gene clusters is relatively poorly understood. In addition to O1 and O17 reported by other laboratories, and the 12 O serogroups (O2, O10, O12, O23, O25, O26, O32, O33, O34, O66, O75, and O76) reported previously by us, in the present study, nine new P. shigelloides serogroups (O8, O17, O18, O37, O38, O39, O44, O45, and O61) were sequenced and annotated. The genes for the O-antigens of these nine groups are clustered together in the chromosome between rep and aqpZ. Only O38 possesses the wzm and wzt genes for the synthesis and translocation of O-antigens via the ATP-binding cassette (ABC) transporter pathway; the other eight use the Wzx/Wzy pathway. Phylogenetic analysis using wzx and wzy showed that both genes are diversified. Among the nine new P. shigelloides serogroups, eight use wzx/wzy genes as targets. In addition, we developed an O-antigen-specific PCR assay to detect these nine distinct serogroups with no cross reactions among them.

Effect of Nanoparticle Size on the Interface Bond Energy in KTa0.5Nb0.5O3 / Polyimide Composites

Chuntian Chen,Juan Wang,Wenlong Yang,Xiaokang Li,Jingyun Zhang 보안공학연구지원센터 2014 International Journal of Hybrid Information Techno Vol.7 No.6

The effects of particle size on the interface bond energy in KTa0.5Nb0.5O3/ polyimide composites were investigated using the molecular dynamics method. The composites of polyimide and the KTa0.5Nb0.5O3 nanoparticles with different size (0.4 nm, 0.5 nm, 0.6 nm, 0.8 nm, 1.2 nm, and 1.4 nm) have been established and simulated using the Forcite program package of Materials Studio software. The calculated results show that the interface bond energy is 38~150 Kcal/mol, which indicate the Van der Waals force exist between the nanoparticle and polyimide matrix. The interface bond energy of the smaller nanoparticle is greater than the lager nanaparticle due to the size effect. The introduction of the smaller nanoparticle could generate the stronger intermolecular bonding interaction between the nanoparticle and polyimide matrix.