Impact of Low Versus Conventional Doses of Chemotherapy During Transcatheter Arterial Chemo-embolization on Serum Fibrosis Indicators and Survival of Liver Cancer Patients

Kong, Wei-Dong,Cao, Jian-Ming,Xu, Jian,Chen, Bo,Yang, Tao,Xu, Tan-Tan,Lu, Guang-Ming,Li, Jun,Huang, Xin-En Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Objectives: To explore the impact of low- vs conventional-dose chemotherapy via transcatheter arterial chemo-embolization (TACE) on serum fibrosis indicators and treatment efficacy of hepatocellular cancer patients (HCC). Materials and Methods: Patients fulfilling the eligibility criteria were assigned to TACE in Group A (with low-dose chemotherapy) or Group B (conventional-dose chemotherapy). Four serum fibrosis related indicators, hyaluronic acid(HA), human pro-collagen type-III (hPC-III), laminin (LN), and collagen type-IV(IV-C) before TACE were compared with the values 7 days after TACE. The response rate and survival time were also compared between the two groups. Results: Fifty patients with HCC were enrolled in this study, including 25 in Group A and 25 in Group B. No significant differences were detected between the two groups in the four indicators before TACE. After TACE, the value of the four serum indicators increased significantly in Group B. However, no significant differences regarding these four indicators were found in Group A after TACE. Significant differences were demonstrated between the two groups after TACE, but median survival time and 1 or 2 year overall survival rates did not differ (P>0.05). Conclusions: Low-, compared with conventional-dose chemotherapy exerts the same impact on the variation of fibrosis related indicators and has no influence on median survival time and survival rate after TACE in HCC patients.

Poor Prognosis Significance of Pretreatment Thrombocytosis in Patients with Colorectal Cancer: a Meta-Analysis

Zhao, Jian-Meng,Wang, Yong-Hong,Yao, Nan,Wei, Kong-Kong,Jiang, Lei,Hanif, Shahbaz,Wang, Zi-Xia Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.9

Background: Recently, several studies have reported that elevated platelet counts may be associated with the poor prognosis of colorectal cancer. However, conclusions remain controversial. This meta-analysis was therefore designed to analyze and evaluate the prognostic role of preoperative or pretreatment thrombocytosis in patients with colorectal cancer. Materials and Methods: We searched PubMed, EMBASE, the Cochrane Library and Web of Science to March 29th, 2015. The citation lists of included studies were also hand-searched to identify further relevant trials. To investigate the association between thrombocytosis and prognosis of colorectal cancer, the 1-year, 3-year and 5-year survival of each studies were obtained. The odds ratio (OR) with its 95% confidence interval (CI) was used to evaluate the relation of overall survival (OS) between thrombocytosis and normal platelet counts (PLT). Likewise, disease free survival (DFS) was obtained and evaluated. The analysis was performed and assessed using Review Manager 5.2. Results: A total of 14 studies (N=5,566 participants, 11 including 4,468 for OS, 6 including 1,533 for DFS) were included in this meta-analysis, of which seven (N=3810) defined thrombocytosis as a platelet count ${\geq}400{\times}10^9/L$, and 375 (9.8%) patients exhibited pretreatment thrombocytosis. Thrombocytosis have a close relationship with the poor OS of colorectal cancer compared with normal PLT, with the pooled ORs of 1-year, 3-year and 5-year survival being 0.41 [95% CI 0.34-0.51; P<0.001], 0.28 [95% CI 0.21-0.38; P<0.001] and 0.26 [95% CI 0.20-0.34; P<0.001], respectively. For DFS, the same results were showed as the pooled ORs of 1-year, 3-year and 5-year survival respectively being 0.34 [95% CI 0.24-0.50; P<0.001], 0.31 [95% CI 0.23-0.43; P<0.001] and 0.25 [95% CI 0.18-0.34; P<0.001]. Conclusions: This meta-analysis indicated that thrombocytosis may predict poor prognosis for patients with colorectal cancer, and platelet counts may be a cost-effective and noninvasive marker.

자폐스펙트럼장애(ASD)에 대한 뇌과학적 접근

정민영(Minyoung Jung),Jian Kong(Jian Kong ),Hirotaka Kosaka(Hirotaka Kosaka ) 한국특수교육학회 2019 한국특수교육학회 학술대회 Vol.2019 No.-

연구목적: 본 연구의 목적은 자폐스펙트럼장애아동의 생물학특징(Biomarker)을 뇌과학적 접근으로 설명하고 교육현장과 임상현장에서 응용 가능성을 밝히는 것이다. 연구방법: 미국·일본·중국의 7-15세의 Autism Spectrum Disorder (ASD) 남아123명, Attention-Deficit Hyperactivity Disorder (ADHD) 남아124명, ASDA·DHD와의 연령과IQ를 일치시킨 전형발달 남아 189 명을 대상으로 자기 공명 영상 (Magnetic Resonance Imaging : MRI)을 촬영하여 뇌기능·구조분석이 통계처리 되었다. 연구결과: ASD는 동일한 나이와 IQ를 가진 전형발달남아에 비하여 후두엽 (lateral occipital cortex)의 발달에 유의한 차이를 나타내었다. 또한 ASD는 동일한 나이와 IQ를 가진 ADHD에 비교하여 섭엽(insula)의 발달에 유의한 차이를 나타내었다. 결론: 본 연구는 교육현장, 임상현장에서도 활용가능한 생물학적 진단기준과 특징을 명확히 하였으며, ASD와 ADHD에 대한 생물학적 이해를 제공하였다.

Tetrazolium Violet Induced Apoptosis and Cell Cycle Arrest in Human Lung Cancer A549 Cells

( Jian Mei Lu ),( Xiao Hong Zhang ),( Nan Zhang ),( Qing Zhong Kong ),( Yun Feng Zhao ) 한국응용약물학회 2012 Biomolecules & Therapeutics(구 응용약물학회지) Vol.20 No.2

Tetrazolium violet is a tetrazolium salt and has been proposed as an antitumor agent. In this study, we reported for the fl rst time that tetrazolium violet not only inhibited human lung cancer A549 cell proliferation but also induced apoptosis and blocked cell cycle progression in the G1 phase. The results showed that tetrazolium violet signifl cantly decreased the viability of A549 cells at 5-15 M. Tetrazolium violet -induced apoptosis in A549 cells was confl rmed by H33258 staining assay. In A549, tetrazolium violet blocked the progression of the cell cycle at G1 phase by inducing p53 expression and further up-regulating p21/WAF1 expression. In addition, an enhancement in Fas/APO-1 and its two forms of ligands, membrane-bound Fas ligand (mFasL) and soluble Fas ligand (sFasL), as well as caspase, were responsible for the apoptotic effect induced by tetrazolium violet. The conclusion of this study is that tetrazolium violet induced p53 expression which caused cell cycle arrest and apoptosis. These fl ndings suggest that tetrazolium violet has strong potential for development as an agent for treatment lung cancer.

Detection of the expression of a Bombyx mori Atypical Protein Kinase C in BmPLV-Infected Larval Midgut

( Jian Cao ),( Yuan Qing He ),( Guo Hui Li ),( Ke Ping Chen ),( Jie Kong ),( Feng Hua Wang ),( Jing Shi ),( Qin Yao ) 한국잠사학회 2011 International Journal of Industrial Entomology Vol.22 No.2

Protein kinase C (PKC) is involved in many cellular signaling pathways, it participates in many physiological processes, such as cell cycle, growth, proliferation, differentiation and apoptosis. To investigate the effect of PKC on the silkworm midgut tissue infection of Bombyx mori parvo-like virus (BmPLV), a B. mori atypical protein kinase C (BmaPKC) gene was cloned from larval midgut tissue, expressed in E. coli and purified. Additionally, the BmPLV susceptible silkworm strain and resistant silkworm strain were used to test the effect of the B. mori infection on BmPLV. The result showed that BmaPKC encodes a predicted 586 amino acid protein, which contains a C-terminal kinase domain and an N-terminal regulatory domain. The maximum expression amount of the soluble (His)6-tagged fusion protein was detected after 0.8 mmol/L IPTG was added and cultured at 21˚C. The (His) 6-tagged fusion protein revealed about 73 kDa molecular weight which confirmed by western blot and mass spectrography. Furthermore BmaPKC protein were detected at 0-72 h post-infection in BmPLVinfected larval midgut tissue, western blot showed that as time went on, the expression of BmaPKC increased gradually in susceptible strain, the expression quantity on 72 h is 5 times of 0 h. However, in resistant strain, the expression quantity is slightly lower than susceptible strain. But no significant change in resistant strain was observed as time went on. The available data suggest that BmaPKC may involve in the regulation of BmPLV proliferation.

Empirical Analysis on the Influence Factors among RCEP Members in a Gravity Model

Jian Zhang,Lingming Kong,Yeong-Gil Kim 동아시아무역학회 2021 Journal of East Asian Trade(JEAT) Vol.3 No.1

Purpose - The purpose of this article is exploring the effect of the RCEP to jointly act against trade protectionism as the international public goods for trade cooperation among countries in the Pacific Rim. Design/Methodology/Approach - Based on the trade gravity model, this article studies the enhancement effect of RCEP on members’ trade potential. It also analyzes on current development needs of RCEP members with indices such as national competitiveness index and international business environment index and analyzes the influence of external environment for trade on the construction of RCEP based on the trade outlook indictor. Findings - This article illustrates the internal necessity of cooperation between RCEP members and analyzes the urgent influence of the deterioration of external trade environment on the construction of RCEP. The results of empirical analysis confirm that trade potential, among member economies increased notably after RCEP was proposed, exhibiting a trend to high trade potential from trade potential exploiting. Research Implications - This study has significance in developing deeper, practical and formal trade cooperation among RCEP members. It also provides some suggestions for China to actively participate in the construction of the RCEP.

Detection of the expression of a Bombyx mori Atypical Protein Kinase C in BmPLV-Infected Larval Midgut

Cao, Jian,He, Yuanqing,Li, Guohui,Chen, Keping,Kong, Jie,Wang, Fenghua,Shi, Jing,Yao, Qin Korean Society of Sericultural Science 2011 International Journal of Industrial Entomology Vol.22 No.2

Protein kinase C (PKC) is involved in many cellular signaling pathways, it participates in many physiological processes, such as cell cycle, growth, proliferation, differentiation and apoptosis. To investigate the effect of PKC on the silkworm midgut tissue infection of Bombyx mori parvo-like virus (BmPLV), a B. mori atypical protein kinase C (BmaPKC) gene was cloned from larval midgut tissue, expressed in E. coli and purified. Additionally, the BmPLV susceptible silkworm strain and resistant silkworm strain were used to test the effect of the B. mori infection on BmPLV. The result showed that BmaPKC encodes a predicted 586 amino acid protein, which contains a C-terminal kinase domain and an N-terminal regulatory domain. The maximum expression amount of the soluble (His)6-tagged fusion protein was detected after 0.8 mmol/L IPTG was added and cultured at $21^{\circ}C$. The (His) 6-tagged fusion protein revealed about 73 kDa molecular weight which confirmed by western blot and mass spectrography. Furthermore BmaPKC protein were detected at 0-72 h post-infection in BmPLVinfected larval midgut tissue, western blot showed that as time went on, the expression of BmaPKC increased gradually in susceptible strain, the expression quantity on 72 h is 5 times of 0 h. However, in resistant strain, the expression quantity is slightly lower than susceptible strain. But no significant change in resistant strain was observed as time went on. The available data suggest that BmaPKC may involve in the regulation of BmPLV proliferation.

Relationships of uPA and VEGF Expression in Esophageal Cancer and Microvascular Density with Tumorous Invasion and Metastasis

Jiang, Jian-Tao,Zhang, Lan-Fang,Zhou, Bin,Zhang, Shun-Qun,Li, Shao-Min,Zhang, Wei,Zhang, Jin,Qiao, Zhe,Kong, Ran-Ran,Ma, Yue-Feng,Chen, Sheng Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Objective: To investigate uPA and VEGF expression in esophageal cancer and relations with tumorous invasion and metastasis. Methods: Immunohistochemistry was used to detect uPA and VEGF expression in the normal epithelial tissue of esophageal mucosa and cancer tissue and detect CD34 labeled micrangium and analyze the relationships with clinical pathological features and tumor angiogenesis. Results: Positive rates for uPA and VEGF protein expression were significantly greater in esophageal cancer than normal epithelial tissue (P < 0.05), the two being linked (P <0.05). In addition, uPA and VEGF protein expression of the high microvessel density (MVD) group was significantly lower than in the low MVD group (P < 0.05), with relation to clinical pathological staging, differentiation and lymph node metastasis (P < 0.05). Conclusion: In esophageal cancer tissue, uPA and VEGF proteins are overexpressed and promote tumor angiogenesis, indicative of a poor prognosis.

A Novel Image Segmentation Method Based on Improved Intuitionistic Fuzzy C-Means Clustering Algorithm

( Jun Kong ),( Jian Hou ),( Min Jiang ),( Jinhua Sun ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.6

Segmentation plays an important role in the field of image processing and computer vision. Intuitionistic fuzzy C-means (IFCM) clustering algorithm emerged as an effective technique for image segmentation in recent years. However, standard fuzzy C-means (FCM) and IFCM algorithms are sensitive to noise and initial cluster centers, and they ignore the spatial relationship of pixels. In view of these shortcomings, an improved algorithm based on IFCM is proposed in this paper. Firstly, we propose a modified non-membership function to generate intuitionistic fuzzy set and a method of determining initial clustering centers based on grayscale features, they highlight the effect of uncertainty in intuitionistic fuzzy set and improve the robustness to noise. Secondly, an improved nonlinear kernel function is proposed to map data into kernel space to measure the distance between data and the cluster centers more accurately. Thirdly, the local spatial-gray information measure is introduced, which considers membership degree, gray features and spatial position information at the same time. Finally, we propose a new measure of intuitionistic fuzzy entropy, it takes into account fuzziness and intuition of intuitionistic fuzzy set. The experimental results show that compared with other IFCM based algorithms, the proposed algorithm has better segmentation and clustering performance.

Pinning Synchronization of Complex Dynamical Networks on Time Scales

Fangdi Kong,Jian-Ping Sun 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.2

The purpose of this paper is to investigate the synchronization problem of complex dynamical networks on time scales, which includes the synchronization problem of continuous-time and discrete-time complex dynamical networks as special cases. A pinning control strategy is designed to achieve synchronization of complex dynamical networks on time scales. Based on the theory of calculus on time scales and the Lyapunov method, pinning synchronization criteria for complex dynamical networks on time scales are established. Moreover, a numerical example is given to verify the effectiveness of theoretical results.