심한 골병변으로 발현된 기능성 낭종성 부갑상선 선종

전숙,김영희,박지영,고관표,박철영,김덕윤,우정택,김성운,김진우,김영설,고석환 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.2

낭종성 부갑상선 선종과 심한 골병변을 동반한 부갑상선 기능항진증은 매우 드문 질환으로서, 저자들은 양측 고관절의 통증을 초기 주소로 내원한 환자에서 고칼슘혈증과 부갑상선 호르몬 증가, 골병변의 방사선적 소견을 통해 부갑상선 기능항진증을 진단하고, 경부 초음파와 컴퓨터 단층 촬영, 부갑상선 스캔검사 및 수술중 부갑상선 낭종액 검사 등을 통해 기능성 부갑상선 낭종의 한 종류인 낭종성 부갑상선 선종을 진단하고 수술적 제거를 통하여 정상화된 1예를 경험하였다. A cystic parathyroid adenoma is rare. A case of primary hyperparathyroidism, with the cystic formation of a parathyroid adenoma and a severe bony lesion, is reported. A 52-year-old male was admitted due to pain in both hips and for evaluation of hypercalcemia. The plasma level of the intact parathyroid hormone (iPTH) was elevated to 1424 pg/mL. Ultrasonography and the computed tomography revealed a parathyroid cyst on the left thyroid lower pole. Parathyroid scintigraphy detected a parathyroid adenoma. A radiograph showed a subperiosteal bone resorption on the phalanges, and a brown tumor (osteitis fibrosa cystica) on the femur shaft was noted. A surgical excision of the parathyroid adenoma was performed. The PTH level in the cystic fluid was increased. A histological examination confirmed a cystic parathyroid adenoma. The PTH level was normalized after the operation (J Kor SOC Endocrinol 18:214-220, 2003).

세미모노코크 구조의 라멘解析

김천욱,전흥재,김지홍 연세대학교 산업기술연구소 1989 논문집 Vol.21 No.1

In this study a semimonocoque element which is composed of rigid frames and a plate is investigated. The structural behavior of the plate subjected to shear is substituted by two equivalent diagonal bars. Applying the equivalent structural elements the semimonocoque structure is formulated into the equivalent rigid frame structure. Numerical analyses of the two types of car-body-structure are performed with the rigid frame program. The numerical results of the present study are compared with those of SAP V-2 program and they are in good agreement within 1% and 2% for the displacements and the bending moments, respectively. The displacements at the load application points and the bending moments of this study agreed with those of experiments in the range of 8% and 7%, respectively.

車體構造解析硏究

김지홍,김천욱,이신영 연세대학교 산업기술연구소 1988 논문집 Vol.20 No.1

An analysis on the load-bearing capacity of floor frame and body structure is investigated by the three dimensional rigid frame analysis. The load-bearing capacity of the body structure is estimated on the basis of comparison to the floor-frame-only structure. The location and magnitude of the maximum displacement are also determined and show good agreement with those of a previous study.In the present study the load-bearing capacity of the floor frame of van-type structures is about 80 percent of the total loadings.

통계적 실험계획에 의한 PECVD로 증착된 실리콘 산화막의 특성 분석

李晟準,金洸範,崔鑛泉,漢秀一,尹知原,金劉邏,朴宰賢,洪尙眞 명지대학교 산업기술연구소 2006 産業技術硏究所論文集 Vol.25 No.-

According as the current needs of low temperature in semiconductor manufacturing process, PECVD using low temperature and high deposition rate is becoming more of a concern. However, PECVD equipment has the defect possibility on the interface between substrates and gate oxide layer, because ions or electrons with much high energy clash the interface. In this study, we embarked on the experiment with Statistical Design of Experiment and then also analyze which parameters influence on the characteristic of silicon dioxide filems. We finally made a constructive proposal for process optimization conditions.

SELDI-TOF MS를 이용한 탄저 치사독소 처리에 의한 생쥐 재식세포주의 단백질체 분석

정경화,서귀문,김성주,김지천,채영규 한양대학교 이학기술연구소 2007 이학기술논문지 Vol.10 No.-

탄저는 탄저균이 동물에 감염되어 발병하는 인수 공통 질병이다. 탄저균의 아포가 감염되면 식균작용에 의해 대식세포 내로 들어가게 된다. 대식세포 내에서 아포는 발아하며 대식세포에서 숙주의 체내로 들어가게 된다. 치사독소는 방어항원과 치사요소로 구성되어있다. 방어항원은 세포의 탄저독소 수용체에 결합하여 방어항원에 결합한 치사요소를 세포내로 운반한다. 운반된 치사요소는 mitogen-activated protein kinase kinase (MAPKK)의 N-말단 부위를 전달하는 것으로 알려졌으나, 이 기작은 세포 사멸에 직접적인 영향을 주지 않는다. 탄저 치사독소에 의한 세포 사멸에 대한 많은 연구가 이루어지고 있으나, 그 기전이 아직까지 명확하게 규명이 되지 않고 있는 실정이다. 본 연구에서는 탄저 치사독소 처리에 의한 생쥐 대식세포주 (RAW 264.7)의 단백질의 발현양상을 조사하여 탄저 치사독소의 세포내 작용기작을 규명하고자 하였다. 단백질 발현 양상을 SELDI-TOF MS을 이용하여 분석하였다. 치사독소를 처리한 대식세포주에서 3,973 Da 단백질의 발현은 90분과 180분에 2배 정도 증가하였다. 단백질 6,066 Da의 발현은 60분에 두 배 증가하고 시간이 지남에 따라 점점 증가하였다. 단백질7,520 Da은 발현이 감소하고, 단백질 8,375 Da과 16,769 Da의 발현은 점점 감소하여 180분에는 절반으로 감소하는 경향을 보였다. MAPKKs가 절단되는 60분이 기준으로 점차 변화가 일어나는 이들 단백질은 치사독소의 절단된 MAPKKs에 의해 발현이 변화하는 것으로 추정되었다. Anthrax is an infectious disease caused by Bacillus anthracis. The spores of B. anthracis are accessed into the body and germinated in macrophages. B. anthracis secretes three virulence factors (such as, protective antigen : PA, lethal factor : LF, and edema factor : EF), and escapes destruction and lyse the macrophages by an unknown mechanism. Anthrax toxins play a central role in pathogenesis of anthrax. Lethal toxin (LeTx) is a mixture of PA and LF. LF is a zinc-dependent endoproteinase that cleaves the amino terminus of mitogen-activated protein kinase kinases (MAPKKs). The role of LeTx in mediating these effects is unknown, largely due to the difficulty in identifying and assigning function to individual proteins. To analyze the cytosolic protein profile of murine macrophages treated with LeTx, we have performed surface enhanced laser desorption/ionization time-of-flight mass spectrometer. The protein peak of 3,973 Da was increased 2 fold at 90 min and 180 min in murine macrophages treated with the LeTx. The protein peak of 6,066 Da was increased 2 fold at 60 min and gradually increased at 90 min and 180min. Protein peak 8,973 Da and 16,769 Da was decreased 2 fold in 180 min. Our results suggest that these proteomic approaches are a useful tool to study gene and protein expression in intoxicated macrophages.

A Study for improvement of identification test in The Korean Pharmacopoeia

Ji Won Kim,Kwang Moon Lee,Younhyoung Lim,Jongpill Lee,Young Hoon shim,Minjung Cho,Sun Hee Kim,Jaesuk Yun,Peol A Kim,Lan Choi,Ho Kim,Nam Hee Kim,Jung Hyun Kim,Chong Ryun Chon,Shin Jung Kang,Bo Kyung Ch 한국에프디시규제과학회(구 한국에프디시법제학회) 2013 한국에프디시법제학회 학술대회 Vol.2013 No.1

A library of TAL effector nucleases spanning the human genome

Kim, Yongsub,Kweon, Jiyeon,Kim, Annie,Chon, Jae Kyung,Yoo, Ji Yeon,Kim, Hye Joo,Kim, Sojung,Lee, Choongil,Jeong, Euihwan,Chung, Eugene,Kim, Doyoung,Lee, Mi Seon,Go, Eun Mi,Song, Hye Jung,Kim, Hwangbeo Nature Publishing Group, a division of Macmillan P 2013 Nature biotechnology Vol.31 No.3

Transcription activator–like (TAL) effector nucleases (TALENs) can be readily engineered to bind specific genomic loci, enabling the introduction of precise genetic modifications such as gene knockouts and additions. Here we present a genome-scale collection of TALENs for efficient and scalable gene targeting in human cells. We chose target sites that did not have highly similar sequences elsewhere in the genome to avoid off-target mutations and assembled TALEN plasmids for 18,740 protein-coding genes using a high-throughput Golden-Gate cloning system. A pilot test involving 124 genes showed that all TALENs were active and disrupted their target genes at high frequencies, although two of these TALENs became active only after their target sites were partially demethylated using an inhibitor of DNA methyltransferase. We used our TALEN library to generate single- and double-gene-knockout cells in which NF-κB signaling pathways were disrupted. Compared with cells treated with short interfering RNAs, these cells showed unambiguous suppression of signal transduction.

Manufacture of Functional Koumiss supplemented with Cichorium intybus L. (chicory) Extract - Preliminary Study

Kim, Dong-Hyeon,Jeong, Dana,Oh, Yong-Taek,Kim, Hong-Seok,Kim, Yun-Gyeong,Song, Kwang-Young,Kang, Il-Byung,Kim, Young-Ji,Park, Jin-Hyeong,Chang, Ho-Seok,Lim, Hyon-Woo,Chon, Jung-Whan,Kim, Hyunsook,Jeon Korean Society of Dairy Science and Biotechnology 2017 한국유가공기술과학회지 Vol.35 No.1

Made using a natural mixed starter of lactic acid bacteria and yeast, Koumiss is a slightly alcoholic fermented mare's milk beverage, and a traditional drink of the nomadic populations of Central Asia. Cichorium intybus L. (chicory) is a sedative with potential cardioactive properties, and its oligosaccharides are beneficial in maintaining healthy gastrointestinal flora. Hence, in this study, we have generated a functional Koumiss containing two different concentrations of chicory. After fermentation of the Koumiss premix, the TA increased to 0.85~0.88%, and the pH decreased to ~4.3. The addition of either concentration of chicory had no significant effect on pH and TA. However, the taste, flavor, color, texture, and overall acceptability decreased in proportion to the added amount of chicory. This study has provided the first data on Koumiss supplemented with chicory. The results could be useful in developing high-quality Koumiss with functional activity using chicory, and allowing large-scale industrial production. Further studies are needed to determine if chicory root extract is beneficial for lifestyle-related diseases.

Improvement of Karmali Agar by Supplementation with Tazobactam for Detecting Campylobacter in Raw Poultry

KIM, YOUNG-JI,WHAN, CHON-JUNG,KIM, HONG-SEOK,KIM, KWANG-YEOP,YIM, JIN-HYEOK,CHO, SEUNG-HAK,SEO, KUN-HO ational Association for Food Protection 2016 Journal of food protection Vol.79 No.11

<B>ABSTRACT</B><P>In this study, Karmali agar was modified by adding tazobactam (T-Karmali agar) to suppress the growth of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli, which frequently contaminates raw poultry meat. By inoculating 30 Campylobacter spp. strains and 25 ESBL-producing E. coli strains onto Karmali agar and T-Karmali agar containing various concentrations of the antibacterial agent, we determined the optimum concentration of tazobactam to be 4 mg/liter. The Campylobacter spp. isolation rate on T-Karmali agar (13.3%) was higher than that on Karmali agar (8.3%), although the difference was not significant (P &gt; 0.05). However, T-Karmali agar showed a significantly greater selectivity than Karmali agar, as evaluated by comparing the numbers of contaminated agar plates (20.8 versus 82.5%; P &lt; 0.05) and the growth indexes (1.36 versus 2.83) of competing flora. The predominant competing flora on Karmali and T-Karmali agar were identified as ESBL-producing E. coli. Thus, T-Karmali agar might be effective for determining the real prevalence of Campylobacter in raw poultry and, especially, contamination with ESBL-producing E. coli.</P>

New colorimetric aptasensor for rapid on-site detection of <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> in chicken carcass samples

Kim, Young-Ji,Kim, Hong-Seok,Chon, Jung-Whan,Kim, Dong-Hyeon,Hyeon, Ji-Yeon,Seo, Kun-Ho Elsevier 2018 Analytica chimica acta Vol.1029 No.-

<P><B>Abstract</B></P> <P>Campylobacter is the most common cause of infectious intestinal disease, with nearly all cases caused by two species: <I>C. jejuni</I> and <I>C. coli</I>. We recently reported a gold nanoparticle-based two-stage aptasensing platform, which was improved in the present study for the rapid and on-site detection of both <I>C. jejuni</I> and <I>C. coli</I> in food samples. Compared to the previous platform, the improved platform yielded a more obvious colour change from red to purple due to the aggregation of gold nanoparticles, and does not require additional time or a pH optimization step for the aptamers to be adsorbed onto the gold nanoparticles. Using a highly specific aptamer that binds to live <I>C. jejuni</I> and <I>C. coli</I>, the improved aptasensor was highly effective for testing pure culture samples. The accuracy of the newly developed platform was comparable (<I>p</I> = 0.688) to that of the gold-standard detection method of tazobactam-supplemented culture, whereas it was superior to the official agar-based detection method (<I>p</I> = 0.016) in a validation study with 50 naturally contaminated chicken carcass samples. This is the first study on a colorimetric sensor that targets both live <I>C. coli</I> and <I>C. jejuni</I> in naturally contaminated samples. In addition, we provide the first evidence that both morphological status and the amount of <I>Campylobacter</I> present play key roles in the effectiveness of colorimetric detection. Thus, suitable selection of an antibody or aptamer with consideration of the morphological status of pathogens in samples is essential for direct detection without enrichment. Our data suggest that the sensor developed in this study can provide an excellent screening method, with a reduction in the detection time from 48 h to 30 min after enrichment, thus saving time, labour, and cost.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A 2-stage label-free aptasensing platform was used to detect <I>C.</I> jejuni and <I>C.</I> coli. </LI> <LI> The colorimetric method detected live <I>C.</I> jejuni and <I>C.</I> coli in a chicken meat rinse. </LI> <LI> The complete assay requires only 30 min or less after sample enrichment. </LI> <LI> Sensitivity and specificity were comparable to those of the gold-standard method. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>