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Sang-Cheol Kim,Soo-Young Park,Jae-Hee Hyoun,Hee-Yeong Cho,Young-Jae Lee,Ji-Hoon Kang,Young-Ki Lee,Doek-Bae Park,Eun-Sook Yoo,Hee-Kyoung Kang 한국독성학회 2004 Toxicological Research Vol.20 No.4
The present study was undertaken to investigate the effects of mistletoe (Viscum album var. coloratum) growing on Carpinus laxiflora BL. on proliferation and differentiation of HL-60 acute promyelocytic leukemia cells. Aqueous extract and its (NH₄)₂SO₄ saturated fractions of the mistletoe exhibited potent anti-proliferation activity against HL-60 cells. Moreover, when HL-60 cells were treated with 0~30% and 30~70% (NH₄)₂SO₄ saturated fractions of the mistletoe, HL-60 expressed CD 66b or CD 14 cell surface antigens and showed activity to reduce nitroblue tetrazolium, indicating that mistletoe induces the differentiation of HL-60 into granulocytes or monocytes. To understand how mistletoe induces the differentiation, we investigated the expression of molecules for modulating the proliferation and differentiation of leukemia cells, such as c-Myc and myeloblastin. The 0~30% (NH₄)₂SO₄ saturated fraction of the mistletoe reduced the mRNA levels of c-Myc and myeloblastin in a time-dependent manner. The results indicate that the mistletoe induces the differentiation of HL-60 cells via the decrease of c-Myc and myeloblastin expressions. Thus, it is suggested that mistletoe has a therapeutic potential for the treatment of acute promyelocytic leukemia.
Kim, Sang-Cheol,Park, Soo-Young,Hyoun, Jae-Hee,Cho, Hee-Yeong,Lee, Young-Jae,Kang, Ji-Hoon,Lee, Young-Ki,Park, Doek-Bae,Yoo, Eun-Sook,Kang, Hee-Kyoung Korean Society of ToxicologyKorea Environmental Mu 2004 Toxicological Research Vol.20 No.4
The present study was undertaken to investigate the effects of mistletoe (Viscum album var. coloratum) growing on Carpinus laxiflora BL. on proliferation and differentiation of HL-60 acute promyelocytic leukemia cells. Aqueous extract and its $(NH_2)_2SO_4$ saturated fractions of the mistletoe exhibited potent anti-proliferation activity against HL-60 cells. Moreover, when HL-60 cells were treated with 0~30% and 30~70% $(NH_2)_2SO_4$ saturated fractions of the mistletoe, HL-60 expressed CD 66b or CD 14 cell surface antigens and showed activity to reduce nitroblue tetrazolium, indicating that mistletoe induces the differentiation of HL-60 into granulocytes or monocytes. To understand how mistletoe induces the differentiation, we investigated the expression of molecules for modulating the proliferation and differentiation of leukemia cells, such as c-Myc and myeloblastin. The 0~30% $(NH_2)_2SO_4$ saturated fraction of the mistletoe reduced the mRNA levels of c-Myc and myeloblastin in a time-dependent manner. The results indicate that the mistletoe induces the differentiation of HL-60 cells via the decrease of c-Myc and myeloblastin expressions. Thus, it is suggested that mistletoe has a therapeutic potential for the treatment of acute promyelocytic leukemia.
Development of the Bovine Corneal Opacity Test in Korea
( Hyoun Kyoung Lee ),( Kyoung Jin Noh ),( Seung Hyeok Seok ),( Min Won Baek ),( Hui Young Lee ),( Dong Jae Kim ),( Yi Rang Na ),( Sung Hoon Park ),( Dutta Noton Kumar ),( Byoung Hee Lee ),( Bae Hwan K 한국동물실험대체법학회 2007 한국동물실험대체법학회 학술대회집 Vol.2007 No.1
The Bovine Corneal Opacity and Permeability (BCOP) assay have been proposed as reliable alternatives to the Draize test. Wider use of the method requires extensive international harmonization study for the validation of the BCOP assay as alternatives. The aim of this work is to establish the BCOP assay in Korea for the international harmonization. In this study, six chemicals and three cosmetics were selected for the test. The eye irritation of the chemicals has already evaluated by the ECVAM(European Centre for the Validation of Alternative Methods). We evaluate the eye irritation of them by the only bovine corneal opacity in the BCOP assay. The Draize test was also preformed for comparative purpose in three chemicals and three cosmetics. For opacity alone, values obtained for three chemicals showed a correlation of r=0.994 (p=0.001) with our Draize score. A correlation coefficient between our Draize score and the ECVAM`s Draize score was 0.982 (p=0.003). The bovine corneal opacity values of additional three chemicals showed a correlation of r=0.968 (p<0.001) with ECVAM`s Draize score. All of three cosmetics were evaluated as non-irritants by the bovine corneal opacity values and our Draize score. These results indicate the international harmonization of the BCOP assay is possible. In further study, the international harmonization study has to be organized with more chemicals and products in more countries.