Ginsenoside fractions regulate the action of monocytes and their differentiation into dendritic cells

Yeo Jin Lee,Young Min Son,Min Jeong Gu,Ki-Duk Song,Sung-Moo Park,Hyo Jin Song,Jae Sung Kang,Jong Soo Woo,Jee Hyung Jung,Deok-Chun Yang,Seung Hyun Han,Cheol-Heui Yun 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.1

Background: Panax ginseng (i.e., ginseng) root is extensively used in traditional oriental medicine. It is a modern pharmaceutical reagent for preventing various human diseases such as cancer. Ginsenosidesd-the major active components of ginsengdexhibit immunomodulatory effects. However, the mechanism and function underlying such effects are not fully elucidated, especially in human monocytes and dendritic cells (DCs). Methods: We investigated the immunomodulatory effect of ginsenosides from Panax ginseng root on CD14⁺ monocytes purified from human adult peripheral blood mononuclear cells (PBMCs) and on their differentiation into DCs that affect CD4⁺ T cell activity. Results: After treatment with ginsenoside fractions, monocyte levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-10 increased through phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and c-Jun N-terminal kinase (JNK), but not p38 mitogen-activated protein kinase (MAPK). After treatment with ginsenoside fractions, TNF-α production and phosphorylation of ERK1/2 and JNK decreased in lipopolysaccharide (LPS)-sensitized monocytes.We confirmed that DCs derived from CD14⁺ monocytes in the presence of ginsenoside fractions (Gin-DCs) contained decreased levels of the costimulatory molecules CD80 and CD86. The expression of these costimulatory molecules decreased in LPS-treated DCs exposed to ginsenoside fractions, compared to their expression in LPS-treated DCs in the absence of ginsenoside fractions. Furthermore, LPS-treated Gin-DCs could not induce proliferation and interferon gamma (IFN-γ) production by CD4⁺ T cells with the coculture of Gin-DCs with CD4⁺ T cells. Conclusion: These results suggest that ginsenoside fractions from the ginseng root suppress cytokine production and maturation of LPS-treated DCs and downregulate CD4⁺ T cells.

Ginsenoside fractions regulate the action of monocytes and their differentiation into dendritic cells

Yeo Jin Lee,Young Min Son,Min Jeong Gu,Ki-Duk Song,Sung-Moo Park,Hyo Jin Song,Jae Sung Kang,Jong Soo Woo,Jee Hyung Jung,Deok-Chun Yang,Seung Hyun Han,Cheol-Heui Yun 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.3

Background: Panax ginseng (i.e., ginseng) root is extensively used in traditional oriental medicine. It is a modern pharmaceutical reagent for preventing various human diseases such as cancer. Ginsenosidesdthe major active components of ginsengdexhibit immunomodulatory effects. However, the mechanism and function underlying such effects are not fully elucidated, especially in human monocytes and dendritic cells (DCs). Methods: We investigated the immunomodulatory effect of ginsenosides from Panax ginseng root on CD14⁺ monocytes purified from human adult peripheral blood mononuclear cells (PBMCs) and on their differentiation into DCs that affect CD4⁺ T cell activity. Results: After treatment with ginsenoside fractions, monocyte levels of tumor necrosis factor (TNF)-a, interleukin (IL)-6, and IL-10 increased through phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and c-Jun N-terminal kinase (JNK), but not p38 mitogen-activated protein kinase (MAPK). After treatment with ginsenoside fractions, TNF-α production and phosphorylation of ERK1/2 and JNK decreased in lipopolysaccharide (LPS)-sensitized monocytes.We confirmed that DCs derived from CD14⁺ monocytes in the presence of ginsenoside fractions (Gin-DCs) contained decreased levels of the costimulatory molecules CD80 and CD86. The expression of these costimulatory molecules decreased in LPS-treated DCs exposed to ginsenoside fractions, compared to their expression in LPS-treated DCs in the absence of ginsenoside fractions. Furthermore, LPS-treated Gin-DCs could not induce proliferation and interferon gamma (IFN-γ) production by CD4⁺ T cells with the coculture of Gin-DCs with CD4⁺ T cells. Conclusion: These results suggest that ginsenoside fractions from the ginseng root suppress cytokine production and maturation of LPS-treated DCs and downregulate CD4⁺ T cells.

이미지 메이킹 교육프로그램 실시 전과 후의 인식 변화분석 -경기,광주지역 남녀대학생을 중심으로-

진형여 ( Hyung Yeo Jin ) 대한미용문화예술학회 2013 대한미용문화예술학회지 Vol.2 No.2

The purpose of the study is to investigate the awareness, need, and importance of image making education for 20s people who are going to have an interview, find a job, or lead a social life in modern society. In particular, it is meaningful to know which parts of image making education are crucial and what parts of image making education are needed for them. This study firstly analyzes changes in perception of before and after image making education and suggests desirable and numberous contents for developing image making education programs. This study was done with the 20s men and women university students of Kyeonggi and Gwangju and the subjects had no experience of image making education before. The research period of this study was from March 10th, 2011 to June 20th, 2013 and the data used for this study were collected by total 142 students. The collected data were processed by SPSS (Statistical Package for the Social Science) WIN 18.0 program for analysis. As the analysis technique, frequency analysis and percentages were used to identify the general characteristics of participants and cross analysis was carried out to compare before the image making education and after the image making education. The results obtained from the study are as follows. First, when looking at the result after the image making education, 97.2% of students cognize the definition of the image making education than they actually have the image making education before. 100 % of students think that it definitely needs for their social life, interviews, or finding a job which is significantly high frequency. Also, 99.3% of them say they are willing to participate in the image making education if they have a chance. 98.6% of students show positive attitude about the image making education which is helpful for their social life, interviews, and finding a job. Second, when exploring the preference on the need of the image making education after the image making education, this result showed as the following ranks: 1. General manner education (greeting, pose, and gesture) 2. Facial expression education 3. Business manner education (handshake, business card, and a place of honor) 4. Social image education (amicable human relations and teamwork), inner image education (positive thoughts and overcoming sense of inferiority). 5. Telephone manner education (importances and characteristics of response esponse by telephone) Self-expression and acting ability education Solution and an effective plan for own complex 6. Fashion strategies and coordination education 7. Diagnosis of personal colors and hair styling education 8. Makeup and props directing education. Based on the results, it appeared that general manner education such as greeting, pose, and gesture and facial expression education are highly meaningful in the image making education of the participants. All things considered, significances of the research is to identify the awareness of the image making education of 20s university students who are facing with interviewing, seeking a job, and their social life in modern world. These facts and results from this study could suggest the upcoming basic data research and could be helpful.

녹색생활 실천에 따른 가정부문의 이산화탄소 감축잠재량 및 경제적 효과 분석

진형아(Hyung Ah Jin),여소영(So Young Yeo),윤소원(So Won Yoon),김대곤(Dai Gon Kim),서정현(Jeong Hyeon Seo),홍유덕(Yoo Deog Hong),한진석(Jin Seok Han) 한국대기환경학회 2013 한국대기환경학회지 Vol.29 No.5

Ginsenoside fractions regulate the action of monocytes and their differentiation into dendritic cells

Lee, Yeo Jin,Son, Young Min,Gu, Min Jeong,Song, Ki-Duk,Park, Sung-Moo,Song, Hyo Jin,Kang, Jae Sung,Woo, Jong Soo,Jung, Jee Hyung,Yang, Deok-Chun,Han, Seung Hyun,Yun, Cheol-Heui The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.1

Background: Panax ginseng (i.e., ginseng) root is extensively used in traditional oriental medicine. It is a modern pharmaceutical reagent for preventing various human diseases such as cancer. Ginsenosidesd-the major active components of ginsengd-exhibit immunomodulatory effects. However, the mechanism and function underlying such effects are not fully elucidated, especially in human monocytes and dendritic cells (DCs). Methods: We investigated the immunomodulatory effect of ginsenosides from Panax ginseng root on $CD14^+$ monocytes purified from human adult peripheral blood mononuclear cells (PBMCs) and on their differentiation into DCs that affect $CD4^+$ T cell activity. Results: After treatment with ginsenoside fractions, monocyte levels of tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-6, and IL-10 increased through phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and c-Jun N-terminal kinase (JNK), but not p38 mitogen-activated protein kinase (MAPK). After treatment with ginsenoside fractions, TNF-${\alpha}$ production and phosphorylation of ERK1/2 and JNK decreased in lipopolysaccharide (LPS)-sensitized monocytes.We confirmed that DCs derived from $CD14^+$ monocytes in the presence of ginsenoside fractions (Gin-DCs) contained decreased levels of the costimulatory molecules CD80 and CD86. The expression of these costimulatory molecules decreased in LPS-treated DCs exposed to ginsenoside fractions, compared to their expression in LPS-treated DCs in the absence of ginsenoside fractions. Furthermore, LPS-treated Gin-DCs could not induce proliferation and interferon gamma (IFN-${\gamma}$) production by $CD4^+$ T cells with the coculture of Gin-DCs with $CD4^+$ T cells. Conclusion: These results suggest that ginsenoside fractions from the ginseng root suppress cytokine production and maturation of LPS-treated DCs and downregulate $CD4^+$ T cells.

우리나라 여성 20대 전후의 피부색 차이

진형여 ( Hyung Yeo Jin ) 한국미용학회 2013 한국미용학회지 Vol.19 No.2

This study investigated the skin color of 500 Korean women in their twenties with the CIE color system using a color difference meter, Minolta CM-700. Analyzing the measured basic data of the skin color, this study used the independent sample t-test to know the skin color difference of Korean women whose ages are before twenty and whose ages are after twenty. Based on the analysis of the data, this study attempts to seek to build the personal color system of Korean women in their twenties, and to be beneficial for the industrial development of cosmetics. The results of this study are as follows. First, as the result of analyzing the differences of brightness(L) for the skin color of women whose ages are before twenty and whose ages are after twenty, it showed significant difference in the central jaw(L) and the left side of neck(L). Still, there was no significant difference in the other parts such as the central forehead (L), the central forehead(L), the left cheekbone(L), the right cheekbone(L), the left of the central cheek(L), the right of the central chee (L), the central lower jaw(L), the right side of neck(L), the medial right upper arm(L), and the medial right forearm(L) for the skin color of women in their twenties. Second, when looking at the results from analyzing the redness(A) for the skin color of women whose ages are before twenty and whose ages after twenty, there were a few things that we should consider. The result showed statistically significant difference in the left of the central cheek(A), the right of the central cheek(A), the central jaw(A), the left side of neck (A), the medial right forearm(A), and the medial right upper arm(A). However, there was no big difference in the central forehead(A), middle of the forehead(A), the left cheekbone(A), the right cheekbone(A), the central jaw(A), the central lower jaw(A), the right side of neck(A) among women in their twenties. Third, considering the results from analyzing the yellowness(B) for the skin color of women in their twenties, we could find out the facts depending on the body parts. Statically significant difference was found in the central forehead(B), the left cheekbone(B), the right cheekbone(B), the left of the central cheek(B), the right of the central cheek(B), the central jaw(B), the left side of neck(B), the right side of neck(B), the medial right forearm(B), and the medial right upper arm(B). Yet, there was no significant difference in middle of the forehead(B) and the central lower jaw(B). All things considered, this study could be used for building the foundation of a personal color system for women in their twenties as the basic data, and also the data regarding the skin color of women in their twenties can be helpful in making the most of the fashion and beauty industries.

20대 여성의 면접메이크업에 관한 인식도 및 실태조사

진형여 ( Hyung Yeo Jin ),김정희 ( Jeong Hee Kim ) 한국미용학회 2010 한국미용학회지 Vol.16 No.2

This research is focused on image make-up which is considered as an important strategy for a successful job interview in modern society. The result from a job interview make-up survey which targeted 300 Korean women at the age of around 20`s women so as to find out the reality of job interview make-up as well as their understanding about this was summarized as follows; As a result, 52% of the participants answered that the main reason for wearing make-up to their job interviews was to create an attractive and better impression at the first time; thus, it was possible to conclude that the majority of Korean women has realized the importance of the job interview make-up. They also thought that wearing make-up could bringthem better results for their job interviews. Furthermore, most answers from 95.2% of the participants showed that people actually wore make-up before their job interviews and if they did not have enough skills for that, they would be willing to learn or improve their abilities with professionals. In fact, their job interview make-up was usually done with natural bright skin tones, even for eye shadow, blusher and lip techniques. Overall, as a matter of fact, the majority of Korean women agreed with the significance of the job interview make-up style. There were also many positive opinions about wearing make-up for the job interviews. For example, a large number of people will spend their time on developing their job interview make-up abilities if there is a good opportunity. Additionally, to get basic levels. female job interviewees should only practice twice. Finally, the researcher hopes that this survey will help a further research on job interview make-up and can be a good model of study for the evolution of their future job interview makeup service and education.

도시 및 농촌 노인의 한국판 MMSE 점수에 사회인구학적 변인이 미치는 영향에 관한 연구

여형용(Hyung Yong Yeo),이강욱(Kang Yk Lee),이군희(Gun Hee Lee),주진형(Jin Hyung Jhoo),김기웅(Ki Woong Kim),이정희(Jung Hie Lee),하규섭(Kyoo Seob Ha),정도언(Do Un Jeong),김용식(Yong Sik Kim),우종인(Jong Inn Woo) 한국정신병리진단분류학회 1998 精神病理學 Vol.7 No.1

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Transduced PEP-1-Grb7 Fusion Protein Suppressed LPS-induced COX-2 Expression

An, Jae-Jin,Kim, So-Young,Lee, Sun-Hwa,Kim, Dae-Won,Ryu, Hea-Jin,Yeo, Seung-Il,Jang, Sang-Ho,Kwon, Hyung-Joo,Kim, Tae-Yoon,Lee, Sang-Chul,Poo, Ha-Ryoung,Cho, Sung-Woo,Lee, Kil-Soo,Park, Jin-Seu,Eum, W Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.2

Although the incidence and severity of atopic dermatitis (AD) is steadily increasing at an alarming rate, its pathogenic mechanisms remain poorly understood yet. Recently, we found that the expression of Grb7 protein was markedly decreased in AD patients using proteomic analysis. In the present study, human Grb7 gene was fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-Grb7 fusion protein. The expressed and purified PEP-1-Grb7 fusion proteins transduced efficiently into skin cells in a time- and dose-dependent manner when added exogenously in culture media. Once inside the cells, the transduced PEP-1-Grb7 protein was stable for 48 h. In addition, transduced PEP-1-Grb7 fusion protein markedly increased cell viability in macrophage RAW 264.7 cells treated with LPS by inhibition of the COX-2 expression level. These results suggest that the PEP-1-Grb7 fusion protein can be used in protein therapy for inflammatory skin disorders, including AD.

Genome-wide identification of chemosensitive single nucleotide polymorphism markers in gastric cancer.

Ha, Ye Jin,Yoon, Sang Nam,Jeon, Yeo Jin,Cho, Dong Hyung,Roh, Seon Ae,Kim, Byung Sik,Kim, Hee Jin,Kim, Seon Young,Kim, Yong Sung,Kim, Jin Cheon Potamitis Press 2011 Anticancer research Vol.31 No.12

<P>A chemosensitive single nucleotide polymorphism (SNP) discovery schema is presented that utilizes (i) genome-wide SNP screening, with a human SNP array and an in vitro chemosensitivity assay, in 93 patients with gastric cancer (GC), and (ii) biological utility assessment using cell viability assays of transfected GC cells. Cytotoxicity analysis showed that most of the MKN1 and SNU638 clones transfected with the G allele of Deoxyribonuclease II beta (DNASE2B) rs3738573 were more sensitive to docetaxel than those with the C allele (p0.001-0.029) and most of the AGS and SNU638 clones transfected with the T allele of 5-hydroxytryptamine receptor IE (HTRIE) rs3828741 were more sensitive to paclitaxel than those with the C allele (p0.001-0.019). Our findings show that the two novel markers, DNASE2B rs3738573 and HTR1E rs3828741, have potential for improving the prediction of chemosensitivity of GC patients.</P>