석이에서 분리한 GE974의 α-Glucosidase 저해효과

최혁재,김남재,김동현 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

This study was conducted to search for the α-glucosidase inhibitor from the natural products. In the previous study, the water extract of Gyrophora esculenta exhibited a potent inhibitory effect on α-glucosidase activities. Then, by bioassay-guided fractionation followed by chromatographic seperation of the water extract of Gyrophora esculenta, α-glucosidase inhibitor was isolated as GE974. GE974 showed significant inhibitory activities on some kinds of α-glucosidases in vitro. Its inhibitory mechanism seemed to be competitive for disaccharides. Also, it markedly inhibited α-glucosidases of intestine separated from both nondiabetics and diabetics.

석이에서 분리한GE974의 혈당상승억제효과

최혁재,김남재,김동현 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

GE974, isolated from Gyrophora esculenta, showed significant inhibitory effect on several α-glucosidases in vitro in previous study. In the present study, GE974 showed significant inhibitory effect on blood glucose elevation in mice loaded with maltose, sucrose, starch and lactose. Also, it exhibited similar effect in alloxan and streptozotocin induced diabetic mice, and genetic diabetic mice(db/db mice) loaded with maltose in dose dependent manner. These results suggest that GE974 may possess hypoglycemic effect that inhibit competitively α-glucosidase activities.

석이중 오르시놀 정량 및 급성독

최혁재,김남재,김동현 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2001 No.-

In previous study, Gyrophora esculenta showed significant inhibitory effect on α-glucosidases in vitro and blood glucose elevation in vivo In the isolating process of active substance, orcinol was separated from Gyrophora esculenta. Orcinol is known to be toxic, therefore, in this study, it was analyzed by the TLC densitometry method for quantitative determination from Gyrophora esculenta. The average amount of orcinol of Gyrophora esculenta was 0.2%. For the purpose of removing orcinol, the water extract of Gyrophora esculenta was sequentially fractionated by organic solvents, and the acute toxicity of each fraction was assessed in mice. Among them, the LD50 of butanol fraction was 1.19 g/kg(p.o.) and the weight increase of mice in that group was somewhat retarded.

한약수치에 관한 연구(제 9보) : 炒甘草 제법의 표준화 및 규격화(1) Preparing Standardization and Regulation of Stir-Frying Glycyrrhzia root(1)

최혁재,이우정,박성환,송보완,김동현,김남재 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

In this study, We carried out the preparing standardization and regulation of processed Glycyrrhizae Radix(PGR) which have been widely used in oriental medicines. Glycyrrhizae Radix(GR) have been generally prepared by the stir-frying, or mix-frying with honey for purpose of decreasing sweetness and augmenting vitality. Firstly, we tried to standardize PGR prepared by the stir-frying. We purchased 14 kinds of PGR and non-processed GR(NPGR) at oriental physician's offices and oriental pharmacies on a nation scale, respectively. The amounts of dry on loss, water extract, diluted ethanol extract, ether extract, total ash, acid insoluble ash, glycyrrhizin(GL), glycyrrhetic acid(GA) and liquiritin(LQ) of them were examined. The amounts of dray on loss, GL and LQ in commercial PGRs showed remarkable decrease, while GA showed increase as compared with NPGR. In order to standardize preparing method of PGR, the effect of heating time on physico-chemical parameters and biological activities were examined. Physico-chemical parameters such as dry on loss, extract amount, GL and LQ contents in PGRs showed decrease, however, GA was increased with heating time as compared with NPGR. Also, GA obtained form heat-treated GR, was found as an artifact in PGRs. PGR was more effective than NPGR in vitro test of DPPH scavenging effect and TBA-Rs reducing effect. PGR and NPGR showed potent hepatoprotective effect on CCl_(1)-intoxicated rats. Especially, PGR prepared by 80 min of heating was the most effective. Considering these results, the optimal condition for PGR preparation was 150℃ for 80min.

주택건설현장 폐 콘크리트 발생 저감 식스시그마 프로젝트 추진 사례 : a Six Sigma Project for Decreasing Waste Concrete in a Housing Construction Site

권혁무,김정택,최준호 한국품질경영학회 2000 품질혁신 Vol.1 No.1

본 사례는 국내 APT 건설 현장에서 수행된 식스시그마 프로젝트에 대한 것으로 페콘크리트 발생 저감 및 잔량발생에 영향을 미치는 주요 인자들을 파악하고 개선안을 도출하는 과정을 보였다. 프로젝트는 식스시그마의 방법론을 따라 정의, 측정, 분석, 개선, 관리의 절차에 따라 주요 변수를 규명하고 개선안을 찾아내는 식으로 진행되었다. 분석결과 페콘크리트 발생을 줄이기 위해서는 첫째, 거푸집 시공 및 타설 프로세스를 개선하고 둘째, 압송관 및 펌프카 내 잔여 레미콘을 최소한으로 감소시키며 셋째, 최종적으로 남은 레미콘을 재활용할 수 있도록 하는 세가지로 요약되었다.

교통사고 발생 추이에 관한 연구

양혁준,최봉근,윤태영,최중명,박순영,유동준 慶熙大學校 醫科大學 1997 慶熙 醫大 論文集 Vol.22 No.1

북한이탈주민의 생활실태 조사 : 경기도 부천시 원미구 춘의임대아파트 거주자를 중심으로

오봉욱, 최종복, 권혁철 가톨릭대학교 사회복지연구소 2010 사회복지리뷰 Vol.15 No.-

Fe_(73.5)Cu₁Nb₃Si_(13.5)B_(9) 초미세 결정립 합금의 분말코아 특성

노태환,최혁열 대한금속재료학회 2004 대한금속·재료학회지 Vol.42 No.6

The melt-spun Fe_(73.5)Cu₁Nb₃Si_(13.5)B_(9) ribbons so called Finemet alloy were annealed in the temperature range of 490 to 610℃ for 1 h and milled by using a planetary ball mill into the powders with the size of 250~850 sm. Then the powder cores were produced through the blending of the magnetic powders with the 5 wt% of ceramic insulator and cold compaction. Further the annealing responses of magnetic, electrical properties and microstructure were investigated. At the annealing temperature of 550℃, the microstructure was transformed from amorphous to crystalline a-Fe phase with the grain size of 10.7 nm, and the electrical resistivity of 117 μΩ-㎝ was obtained. At the same time, the effective permeability and quality factor reached 120 and 50, respectively, and the permeability persisted up to about 500 kHz. Moreover the core loss at the frequency of 50 kHz and the magnetic induction of 0.1 T was about 300 mW/cm³. These magnetic properties were comparable to the conventional powder core materials such as MPP(molybdenum permalloy powder), Sendust etc. However the dc bias characteristics represented by the permeability stability upon dc magnetic field superposition were found to be unsatisfactory. (Received February 16, 2004)

MIC구형 공진기를 이용한 주파수 스캐닝 안테나

박한규,최평석,양혁재 연세대학교 산업기술연구소 1980 논문집 Vol.12 No.2

마이크로스트립 單一共振器를 直列로 연결한 周波數스캐닝 안테나의 製作 및 動作原理에 대하여 고찰하였다. 마이크로스프립 基板의 두께가 1/32inch(0.8mm)이고 比誘電率 εr=2.45를 사용하였을 경우 8.7㎓에서 9.25㎓까지 周波數를 변환시켰을 때 ±30°로 스캐닝 하였으며 이때 共振器 자체의 反射波에 의한 位相偏移가 30%까지 차지하였다. In this paper, the principles and fabrication of the frequency scanning antenna formed by the cascaded array of single resonators are described. As the microstrip substrate, the depth of 1/32 inch and ?? of 2.45 substrate is used and the resultant beam scan angle is 30° from 8.7 ㎓ to 9.25 ㎓ range. It is showed that this scanning is due to the phase shift in a resonator as well as the transmission line and the resonator phase shift responsible for 30% of the total phase shift. Experimental results shows a little discrepancy from the theoretical value and this considered to come from the mis-matching of each resonators.

한약수치에 관한 연구(제 8보) : 오수유 탕포법 The Changes of Physico-chemical Parameter and Biological Activities by Processing of Evodia Fruit

박성환,이우정,최혁재,송보완,김동현,김남재 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

We have studied the physico-chemical change and pharmacological transformation of traditional herbal medicines with processing. Evodia fruit (EF) has processed for the purpose of detoxification and reduction of its bitter taste. There are various methods of processing EF in Oriental medicinal references. Among them, we processed EF according to the method of Sang-han theory, the most famous medicinal reference. We processed EF with washing in hot water, and then dried. Processed EFs(PEFs) were prepared according to the above procedure through repetition of 1,3,5 and 7 times respectively. The contents of weight loss, water extract, diluted ethanol extract, ether extract, total ash, acid insoluble ash, alkaloids and limonin in non-processed EF (NPEF) and PEFs were examined. The weight loss, and contents of water extract and dilute ethanol extract in PEF showed decrease in proportion to increase of washing time, while the contents of ether extract, total ash and acid insoluble ash showed little change as compared with those of NPEF. And the content of evodiamine and rutaecarpine was not changed distinctly. However, the content of limonin decreased in the final processed material in proportion to increase of washing times with hot water. And the intense of bitter taste in PEF was also reduced. These results were ascribed to the flowing-out of the water-soluble portion. The biological activities of NPEF and PEF were also investigated. In the test of DPPH scavenging effect, xanthine oxidase inhibition effect and TBA-Rs effect, PEF was more effective than NPEF in vitro. Also, both NPEF and PEF showed potent analgesic and anti-inflammatory effects against in mice. Especially, PEF by 3 times washing with hot water was more effective than other PEFs. From these results, it is considered that PEF should be prepared by 3 times washing with hot water.