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Lee, Myoung Woo,Kim, Dae Seong,Yoo, Keon Hee,Kim, Hye Ryung,Jang, In Keun,Lee, Ji Hyang,Kim, So Yeon,Son, Meong Hi,Lee, Soo Hyun,Jung, Hye Lim,Sung, Ki Woong,Koo, Hong Hoe Korean Society of Hematology; Korean Society of Bl 2013 Blood Research Vol.48 No.2
<P><B>Background</B></P><P>Because of the heterogeneity of human mesenchymal stem cells (MSCs), methods for cell expansion in culture and the effects on gene expression are critical factors that need to be standardized for preparing MSCs. We investigated gene expression patterns of MSCs with different seeding densities and culture times.</P><P><B>Methods</B></P><P>Bone marrow-derived MSCs were plated at densities from 200 cells/cm<SUP>2</SUP> to 5,000 cells/cm<SUP>2</SUP>, and the gene expression patterns were evaluated over time using a reverse-transcription polymerase chain reaction assay.</P><P><B>Results</B></P><P>The mRNA levels of factors that play a critical role in cell migration and tissue regeneration, such as podocalyxin-like protein (PODXL), α4-integrin, α6-integrin, and leukemia inhibitory factor (LIF), were higher in MSCs plated at 200 cells/cm<SUP>2</SUP> than in MSCs plated at 5,000 cells/cm<SUP>2</SUP>. The mRNA levels of these factors gradually increased for 10 days and then decreased by day 15 in culture. MSCs seeded at 200 cells/cm<SUP>2</SUP> that were cultured for 10 days expressed high levels of Oct-4 and Nanog. Indoleamine 2,3-dioxygenase, cyclooxygenase-1, and hepatocyte growth factor expression were upregulated in the presence of the proinflammatory cytokine interferon-γ in these cells.</P><P><B>Conclusion</B></P><P>We found differences in the gene expression patterns of MSCs under different culture conditions. MSCs from 10-day cultures seeded at a low density were efficiently expanded, expressed PODXL, α6-integrin, α4-integrin, and LIF, and maintained properties like stemness and immunomodulation. Therefore, <I>ex vivo</I> expansion of MSCs maintained for an adequate culture time after plating at low cell density can provide an effective regenerative medicinal strategy for cell therapies using MSCs.</P>
How Textual Sources Affect Fashion Design Ideation and Developing Process
( Eui Young Yang ),( Hoe Ryung Lee ),( Su Jin Park ),( Ji Woon Jeong ),( Hye In Park ),( Jisoo Ha ) 한국의류산업학회 2021 한국의류산업학회지 Vol.23 No.1
The research expects that textual sources such as reading texts with additional information in the form of texts can be effective inspiration sources for fashion design ideation and development process. This research analyzes how efficiently textual sources work along with individual internal sources, such as sociocultural influence, design fixation, and during the design process. Six fashion design graduate students shared 2 inspirational experiences under 2 different studies (4 experiences in total); in addition, in-depth interviews were conducted based on individual design sketches. The result shows that textual sources provided a positive effect on all 6 participants with different intensities based on various backgrounds and individual tastes. This result demonstrates individual ‘influence’ (their sociocultural capital such as personal preferences, likings, habits, and past experiences) and ‘inspiration’ mutually work together to make an effect on fashion designers’ ideation and development process for the design, sometimes one working more than the other (or vice versa), respectively. This paper makes important practical contributions by identifying and discussing the design behavior performed (especially in fashion design) by fashion design students during the design process with new sources of inspiration provided such as textual sources. The research revealed how textual sources can be an effective inspiration for fashion design students and provide insight to fashion design educators and professional fashion designers.
Choi Rihwa,Chun Mi Ryung,Park Jisook,이지원,Ju Hee Young,Cho Hee Won,Hyun Ju Kyung,Koo Hong Hoe,Yi Eun Sang,Lee Soo-Youn 대한진단검사의학회 2021 Annals of Laboratory Medicine Vol.41 No.2
Background: We developed an assay to measure DNA-incorporated 6-thioguanine (DNA-TG) and validated its clinical applicability in Korean pediatric patients with acute lymphoblastic leukemia (ALL) in order to improve individualized thiopurine treatment and reduce the life-threatening cytotoxicity. Methods: The DNA-TG assay was developed based on liquid chromatography-tandem mass spectrometry, with isotope-labeled TG-d3 and guanine-d3 as internal standards. This method was applied to 257 samples of pediatric ALL patients. The DNA-TG level was compared with erythrocyte TG nucleotide (RBC-TGN) level in relation to the TPMT and NUDT15 genotypes, which affect thiopurine metabolism, using Spearman’s rank test and repeated measure ANOVA. Results: For DNA-TG quantification, a linearity range of 10.0-5,000.0 fmol TG/μg DNA; bias for accuracy of –10.4% –3.5%; coefficient of variation for intra- and inter-day precision of 3.4% and 5.8% at 80 fmol TG/μg DNA and of 4.9% and 5.3% at 800 fmol TG/μg DNA, respectively; and recovery of 85.7%-116.2% were achieved without matrix effects or carry-over. The median DNA-TG level in the 257 samples was 106.0 fmol TG/μg DNA (interquartile range, 75.8-150.9). There was a strong correlation between DNA-TG and RBC-TGN levels (ρ=0.68, P<0.0001). The DNA-TG/RBC-TGN ratio was significantly higher in NUDT15 intermediate metabolizers (*1/*2 and *1/*3) than in patients with wild-type alleles (P<0.0001). Conclusions: This simple and sensitive method for measuring DNA-TG level can improve therapeutic drug monitoring for thiopurine treatment.