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Disinfectant effect of monopersulfate (MPS) compound to white spot syndrome virus (WSSV) of shrimp
( Cwr Gunasekara ),( Seok-ryel Kim ),( Lgtg Rajapaksha ),( Shmp Wimalasena ),( Hnks Pathirana ),( Gee-wook Shin ) 한국어병학회 2020 한국어병학회지 Vol.33 No.2
This study investigated the disinfection effect of monopersulfate (MPS) compound against white spot syndrome virus (WSSV) by bioassay using kuruma shrimp (Marsupenaeus japonicas). A WSSV stock was prepared with muscle homogenate from WSSV-infected whiteleg shrimp (Penaeus vannamei) and its lethal dose 50% endpoint (LD<sub>50</sub>) and infectious dose 50% endpoint (SID<sub>50</sub>) were respectively determined as 10<sup>-5.63</sup> and 10<sup>-6.79</sup> in bioassay using kuruma shrimp, followed by PCR assays. The disinfective effect of MPS compound (1.2 ppm, 2.4 ppm, 4.8 ppm) was performed by bioassay using about 10-fold higher dilution (10<sup>-4</sup>) of WSSV homogenate. The compound resulted in WSSV inactivation by a concentration-dependent manner. In addition, 4.8 ppm of MPS completely prohibited WSSV infection. To our knowledge, this study is the first report about the usefulness of MPS as a disinfectant to WSSV.